Mitochondrial Transplantation Therapy against Ifosfamide Induced Toxicity on Rat Renal Proximal Tubular Cells
Drug Res (Stuttg) DOI: 10.1055/a-1967-2066Mitochondrial dysfunction is a basic mechanism leading to drug nephrotoxicity.
Replacement of defective mitochondria with freshly isolated mitochondria is
potentially a comprehensive tool to inhibit cytotoxicity induced by ifosfamide
on renal proximal tubular cells (RPTCs). We hypothesize that the direct exposure
of freshly isolated mitochondria into RPTCs affected by ifosfamide might restore
mitochondrial function and reduce cytotoxicity. So, the aim of this study was to
assess the protective effect of freshly isolated mitochondrial transplantation
against ifosfamide-induced cytotoxicity in RPTCs. Therefore, the suspension of
rat RPTCs (106 cells/ml) in Earle’s solution with the
pH of 7.4 at 37°C was incubated for 2 h after ifosfamide
(4 mM) addition. Fresh mitochondria were isolated from the rat kidney
and diluted to the needed concentrations at 4°C. The media containing
suspended RPTCs was replaced with mitochondrial-supplemented media, which was
exposed to cells for 4 hours in flasks-rotating in a water bath at
37°C. Statistical analysis demonstrated that mitochondrial
administration reduced cytotoxicity, lipid peroxidation (LPO), reactive oxygen
species (ROS) production, mitochondrial membrane potential (MMP) collapse,
lysosomal membrane damage, extrac...
Source: Drug Research - Category: Drugs & Pharmacology Authors: Arjmand, Abdollah Mashhadi, Melika Kaveh, Armin Kamranfar, Farzaneh Seydi, Enayatollah Pourahmad, Jalal Tags: Original Article Source Type: research
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