Imipramine prevents Porphyromonas gingivalis lipopolysaccharide-induced microglial neurotoxicity

In this study, we examined whether P. gingivalis-induced brain inflammation could be ameliorated by pharmacotherapy, using in vivo and in vitro studies. In an animal experiment, peripheral administration of LPS-PG induced inflammation in the hippocampus via microglial activation, which was inhibited by pre-treatment with the antidepressant imipramine. Similarly, LPS-PG-induced inflammation in MG-6 cells, a mouse microglial cell line, was inhibited by pre-treatment with imipramine, which caused imipramine-induced inhibition of NF-κB signaling. Culture media obtained from LPS-PG-treated MG-6 cells induced neuronal cell death in Neuro-2A cells, a mouse neuroblastoma cell line, which was prevented by pre-treatment of MG-6 cells with imipramine. These results indicate that imipramine inhibits LPS-PG-induced inflammatory responses in microglia and ameliorates periodontal disease-related neural damage.PMID:36240654 | DOI:10.1016/j.bbrc.2022.09.109

Source: Biochemical and Biophysical Research communications - October 14, 2022 Category: Biochemistry Authors: Yosuke Yamawaki Hiroki So Kana Oue Satoshi Asano Hisako Furusho Mutsumi Miyauchi Kotaro Tanimoto Takashi Kanematsu Source Type: research