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siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research

The association of ribosomal protein L18 with Newcastle disease virus matrix protein enhances viral translation and replication
In this study, we found that the expression and transcription levels of RPL18 was reduced early in NDV infection but increased later in NDV infection. In addition, the presence of cytoplasmic NDV matrix (M) protein was responsible for the increased expression of RPL18 in both virus-infected cells and plasmid-transfected cells. Moreover, cytoplasmic M protein increased RPL18 expression in a dose-dependent manner, even though they did not interact with each other. Furthermore, siRNA-mediated knockdown of RPL18 or overexpression of RPL18 dramatically reduced or enhanced NDV replication by decreasing or increasing viral protei...
Source: Avian Pathology - December 3, 2021 Category: Pathology Authors: Zhiqiang Duan Hong Tang Yanbi Wang Caiqin Zhao Lei Zhou Yifan Han Source Type: research

H7N9 Influenza A Virus Exhibits Importin- α7–Mediated Replication in the Mammalian Respiratory Tract
The acute respiratory distress syndrome (ARDS) is the leading cause of death in influenza A virus (IAV) –infected patients. Hereby, the cellular importin-α7 gene plays a major role. It promotes viral replication in the lung, thereby increasing the risk for the development of pneumonia complicated by ARDS. Herein, we analyzed whether the recently emerged H7N9 avian IAV has already adapted to human i mportin-α7 use, which is associated with high-level virus replication in the mammalian lung. Using a cell-based viral polymerase activity assay, we could detect a decreased H7N9 IAV polymerase activity when importin-α7 was silenced by siRNA.
Source: American Journal of Pathology - February 8, 2017 Category: Pathology Authors: Stephanie Bertram, Swantje Thiele, Carola Dreier, Patricia Resa-Infante, Annette Preu ß, Debby van Riel, Chris K.P. Mok, Folker Schwalm, Joseph S.M. Peiris, Hans-Dieter Klenk, Gülsah Gabriel Tags: Regular Article Source Type: research

H7N9 Influenza A Virus Exhibits Importin- α7-Mediated Replication in the Mammalian Respiratory Tract.
H7N9 Influenza A Virus Exhibits Importin-α7-Mediated Replication in the Mammalian Respiratory Tract. Am J Pathol. 2017 Feb 08;: Authors: Bertram S, Thiele S, Dreier C, Resa-Infante P, Preuß A, van Riel D, Mok CK, Schwalm F, Peiris JS, Klenk HD, Gabriel G Abstract The acute respiratory distress syndrome (ARDS) is the leading cause of death in influenza A virus (IAV)-infected patients. Hereby, the cellular importin-α7 gene plays a major role. It promotes viral replication in the lung, thereby increasing the risk for the development of pneumonia complicated by ARDS. Herein, we analyzed whether the rec...
Source: The American Journal of Pathology - February 7, 2017 Category: Pathology Authors: Bertram S, Thiele S, Dreier C, Resa-Infante P, Preuß A, van Riel D, Mok CK, Schwalm F, Peiris JS, Klenk HD, Gabriel G Tags: Am J Pathol Source Type: research