The association of ribosomal protein L18 with Newcastle disease virus matrix protein enhances viral translation and replication

In this study, we found that the expression and transcription levels of RPL18 was reduced early in NDV infection but increased later in NDV infection. In addition, the presence of cytoplasmic NDV matrix (M) protein was responsible for the increased expression of RPL18 in both virus-infected cells and plasmid-transfected cells. Moreover, cytoplasmic M protein increased RPL18 expression in a dose-dependent manner, even though they did not interact with each other. Furthermore, siRNA-mediated knockdown of RPL18 or overexpression of RPL18 dramatically reduced or enhanced NDV replication by decreasing or increasing viral protein translation rather than viral RNA synthesis and transcription. Taken together, these results suggested that the increased expression of RPL18 might be associated with the physical clumping together of the M protein, which in turn promoted viral protein biosynthesis and NDV replication, thus revealing for the first time the association of RPL18 with NDV M protein was important for viral translation and replication.PMID:34859725 | DOI:10.1080/03079457.2021.2013435

Source: Avian Pathology - December 3, 2021 Category: Pathology Authors: Zhiqiang Duan Hong Tang Yanbi Wang Caiqin Zhao Lei Zhou Yifan Han Source Type: research