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Stearic acid methyl ester ​promotes migration of mesenchymal stem cells and accelerates cartilage defect repair
Publication date: Available online 23 October 2019Source: Journal of Orthopaedic TranslationAuthor(s): Yamei Liu, Liangliang Xu, Liuchao Hu, Dongfeng Chen, Lijuan Yu, Xican Li, Hongtai Chen, Junlang Zhu, Chen Chen, Yiwen Luo, Bin Wang, Gang LiAbstractMesenchymal stem cells (MSCs) can be easily expanded without losing the ability of multilineage differentiation, including oesteogenic, chondrogenic and adipogenic differentiation. These characters make MSCs a promising cell resource for cartilage defect repair. MSCs could be recruited by inflammatory stimulation, then home to the injury tissues. However, its capacity of homin...
Source: Journal of Orthopaedic Translation - October 24, 2019 Category: Orthopaedics Source Type: research

Chondrocytes Promote Vascularization in Fracture Healing Through a FOXO1 ‐Dependent Mechanism
We examined the role of chondrocytes in angiogenesis during fracture healing and the role of the transcription factor forkhead box ‐O 1 (FOXO1), which upregulates wound healing in soft tissue. Closed fractures were induced in experimental mice with lineage‐specific FOXO1 deletion by Cre recombinase under the control of a collagen‐2α1 promoter element (Col2α1Cre+FOXO1L/L) and Cre recombinase negative control littermates containing flankingloxP sites (Col2 α1Cre–FOXO1L/L). Experimental mice had significantly reduced CD31+ new vessel formation. Deletion of FOXO1 in chondrocytes in vivo suppressed the expression of ...
Source: Journal of Bone and Mineral Research - March 11, 2019 Category: Orthopaedics Authors: Citong Zhang, Daniel Feinberg, Mohammed Alharbi, Zhenjiang Ding, Chanyi Lu, J Patrick O'Connor, Dana T Graves Tags: Original Article Source Type: research

Efficient, Low-Cost Nucleofection of Passaged Chondrocytes
This study identifies a cost-effective, efficient method of nonviral nucleofection of bovine-passaged chondrocytes using known buffer formulations. Human-passaged chondrocytes could also be successfully nucleofected in 1M buffer. Thus this method should facilitate cost-efficient gene targeting of cells used for articular cartilage repair in a research setting.
Source: Cartilage - February 7, 2016 Category: Orthopaedics Authors: Parreno, J., Delve, E., Andrejevic, K., Paez-Parent, S., Wu, P.-h., Kandel, R. Tags: Articles Source Type: research

Small Players Ruling the Hard Game: siRNA in Bone Regeneration
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - February 1, 2016 Category: Orthopaedics Authors: S Ghadakzadeh, M Mekhail, A Aoude, R Hamdy, M Tabrizian Tags: Review Source Type: research

Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model
In an established steroid-associated osteonecrosis (SAON) rabbit model we found recently that blockage Src by siRNA could improve reconstructive repair of osteonecrosis via enhancing osteogenesis and inhibiting bone resorption. The current study investigated if blocking Src was able to prevent steroid-associated osteoporosis (SAOP) in the same SAON animal model. Rabbits were treated with pulsed lipopolysaccharide (LPS) and corticosteroid methylprednisolone (MPS). At 2, 4, 6weeks after induction, Src siRNA, control siRNA and saline were intramedullary injected into proximal femur, respectively.
Source: Bone - November 17, 2015 Category: Orthopaedics Authors: Li-Zhen Zheng, Xin-Luan Wang, Hui-Juan Cao, Shi-Hui Chen, Le Huang, Ling Qin Tags: Original Full Length Article Source Type: research

Blockage of Src by Specific siRNA as a Novel Therapeutic Strategy to Prevent Destructive Repair in Steroid‐Associated Osteonecrosis in Rabbits
This study was designed to prove our hypothesis that blocking VEGF‐Src signaling pathway by specific Src siRNA was able to prevent destructive repair in a SAON rabbit model. Destructive repair in SAON was induced in rabbits. At 2, 4, 6 weeks after SAON induction, VEGF, anti‐VEGF, Src siRNA, Src siRNA + VEGF, control siRNA and saline were intramedullary injected into proximal femora for each group, respectively. Vascularization and permeability were quantified by dynamic contrast‐enhanced (DCE) MRI. At week 6 after SAON induction, proximal femora were dissected for micro‐CT‐based trabecular architecture with f...
Source: Journal of Bone and Mineral Research - April 27, 2015 Category: Orthopaedics Authors: Li‐zhen Zheng, Hui‐juan Cao, Shi‐hui Chen, Tao Tang, Wei‐min Fu, Le Huang, Dick Ho Kiu Chow, Yi‐xiang Wang, James Francis Griffith, Wei He, Hong Zhou, De‐wei Zhao, Ge Zhang, Xin‐luan Wang, Ling Qin Tags: Original Article Source Type: research

Src blockage by siRNA inhibits VEGF-induced vascular hyperpemeability and osteoclast activity – an mechanism study for preventing destructive repair of osteonecrosis
Destructive repair is the pathological feature of ONFH characterized with the elevated vascular permeability and persistent bone resorption, which is associated with higher VEGF expression, activated c-Src, and vascular leakage. Activated c-Src also participates in mediating endothelial permeability and osteoclasts activity. However, the molecular mechanism of the VEGF and c-Src contributing to the destructive repair process remains unknown. The purpose of this study is to delineate the role of VEGF and c-Src in triggering destructive repair of osteonecrosis in vitro, as well as to elucidate if VEGF mediating vascular perm...
Source: Bone - December 29, 2014 Category: Orthopaedics Authors: Hui-Juan Cao, Li-Zhen Zheng, Nan Wang, Lin-Ying Wang, Ye Li, Dan Li, Yu-Xiao Lai, Xin-Luan Wang, Ling Qin Tags: Original Full Length Article Source Type: research

Src blockage by siRNA inhibits VEGF-induced vascular hyperpemeability and osteoclast activity– an mechanism study for preventing destructive repair of osteonecrosis
Destructive repair is the pathological feature of ONFH characterized with the elevated vascular permeability and persistent bone resorption, which is associated with higher VEGF expression, activated c-Src, and vascular leakage. Activated c-Src also participates in mediating endothelial permeability and osteoclasts activity. However, the molecular mechanism of the VEGF and c-Src contributing to the destructive repair process remains unknown. The purpose of this study is to delineate the role of VEGF and c-Src in triggering destructive repair of osteonecrosis in vitro, as well as to elucidate if VEGF mediating vascular perm...
Source: Bone - December 29, 2014 Category: Orthopaedics Authors: Hui-Juan Cao, Li-Zhen Zheng, Nan Wang, Lin-Ying Wang, Ye Li, Dan Li, Yu-Xiao Lai, Xin-Luan Wang, Ling Qin Source Type: research

Involvement of ERCC1 in the pathogenesis of osteoarthritis through the modulation of apoptosis and cellular senescence
In this study we investigated the function of ERCC1 in chondrocytes and its association with the pathophysiology of OA. ERCC1 expression in normal and osteoarthritic cartilage was assessed, as were changes in ERCC1 expression in chondrocytes under catabolic stress. Inhibiting ERCC1 in chondrocytes under interleukin‐1β stimulation using small interfering RNA (siRNA) was also evaluated. Finally, cellular senescence and apoptosis were examined in relation to ERCC1 function. ERCC1 expression was decreased in OA cartilage and increased within 4 h of exposure to interleukin (IL)‐1β, but decreased after 12 h. The inhibi...
Source: Journal of Orthopaedic Research - June 25, 2014 Category: Orthopaedics Authors: Koji Takayama, Yohei Kawakami, Sahnghoon Lee, Nick Greco, Mitra Lavasani, Yutaka Mifune, James H. Cummins, Takashi Yurube, Ryosuke Kuroda, Masahiro Kurosaka, Freddie H. Fu, Johnny Huard Tags: Research Article Source Type: research