Stearic acid methyl ester ​promotes migration of mesenchymal stem cells and accelerates cartilage defect repair

Publication date: Available online 23 October 2019Source: Journal of Orthopaedic TranslationAuthor(s): Yamei Liu, Liangliang Xu, Liuchao Hu, Dongfeng Chen, Lijuan Yu, Xican Li, Hongtai Chen, Junlang Zhu, Chen Chen, Yiwen Luo, Bin Wang, Gang LiAbstractMesenchymal stem cells (MSCs) can be easily expanded without losing the ability of multilineage differentiation, including oesteogenic, chondrogenic and adipogenic differentiation. These characters make MSCs a promising cell resource for cartilage defect repair. MSCs could be recruited by inflammatory stimulation, then home to the injury tissues. However, its capacity of homing is extremely limited. Thus, it has become extremely necessary to develop an agent or a method, which can be used to enhance the efficiency of MSCs homing. In the present study, we found that stearic acid methyl ester (SAME) could promote the migration of MSCs. Interestingly, we found SAME significantly increased the expression levels of Vav1 in MSCs. On the other hand, the enhanced migration ability of MSCs induced by SAME was retarded by Vav1 siRNA and ROCK2 inhibitor. In addition, we also checked the effect of SAME on mobilisation of MSCs in vivo. The results showed that SAME increased the number of MSCs in peripheral blood and enhanced the capacity of colony formation. Finally, using a cartilage defect model in rats, we found SAME could improve cartilage repair. Taken together, our study demonstrates that SAME can enhance MSCs migration ability mainly t...
Source: Journal of Orthopaedic Translation - Category: Orthopaedics Source Type: research