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miR-210-3p regulates the proliferation and apoptosis of non-small cell lung cancer cells by targeting SIN3A.
Authors: Ren J, Li X, Dong H, Suo L, Zhang J, Zhang L, Zhang J Abstract Previous studies have indicated that microRNA (miR)-210-3p is upregulated in NSCLC, however, the specific mechanism underlying the role of miR-210-3p in NSCLC pathogenesis requires further investigation. The aim of the present study was to explore the roles of miR-210-3p in NSCLC and the associated mechanisms. A total of 30 NSCLC tissues and paired adjacent normal tissues were collected for study. Reverse transcription-quantitative polymerase chain reaction was performed to compare the expression of miR-210-3p in the 30 paired cancerous and adj...
Source: Experimental and Therapeutic Medicine - September 28, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer
AbstractIn order to unveil ubiquitin pathway genes (UPGs) that are essential for non-small cell lung cancer (NSCLC) cell proliferation, we recently conducted a siRNA screening experiment to knockdown the expression of 696 UPGs found in the human genome in A549 and H1975 NSCLC cells. We found that silencing of one of the candidates,RFWD3 that encodes an E3 ubiquitin ligase essential for the repair of DNA interstrand cross-links in response to DNA damage, led to dramatic inhibition of NSCLC cell proliferation with significant Z-scores. Knockdown ofRFWD3 suppressed colony forming activity of NSCLC cells.We further evaluated t...
Source: Frontiers of Medicine - September 27, 2019 Category: General Medicine Source Type: research

Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1.
Authors: Ren A, Wen Z, Zheng L Abstract Tumor protein p53-inducible nuclear protein 1 (TP53INP1) is a tumor suppressor associated with malignant tumor metastasis. In addition, it has been reported that hsa-microRNA (miR)-3934 serves key roles in various types of lung cancer, including small-cell lung carcinomas (SCLC) and non-SCLC (NSCLC). Therefore, the present study aimed to determine the effects of miR-3934-5p on cell proliferation and apoptosis, and on sensitivity to cisplatin (DDP). Reverse transcription-quantitative polymerase chain reaction analysis and western blotting were conducted for the analysis of mRN...
Source: Experimental and Therapeutic Medicine - August 16, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

Tip60-siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway.
Authors: Liang Z, Yu Q, Ji H, Tian D Abstract Lung cancer is a leading cause of cancer-associated mortality and morbidity worldwide. Previous studies have suggested that ATP-binding cassette transporter E1 (ABCE1) acetylation is upregulated in the tissues and cells of lung cancer and is associated with the prognosis of patients with lung cancer. The aim of the present study was to investigate the association between Tat interactive protein 60 kDa (Tip60) expression and ABCE1 acetylation, and the effect of Tip60 on the biological functions of A549 lung carcinoma cells. The expression levels of Tip60 and ABCE1 acetyl...
Source: Experimental and Therapeutic Medicine - April 4, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

MiR-125b-1-3p Exerts Antitumor Functions in Lung Carcinoma Cells by Targeting S1PR1.
Conclusion: Our results suggest that miR-125b-1-3p exerts antitumor functions in NSCLC cells by targeting S1PR1. PMID: 30082521 [PubMed - in process]
Source: Chinese Medical Journal - August 9, 2018 Category: General Medicine Authors: Zhang X, Liu Y, Huang WC, Zheng LC Tags: Chin Med J (Engl) Source Type: research

The action mechanism of lncRNA-HOTAIR on the drug resistance of non-small cell lung cancer by regulating Wnt signaling pathway.
Authors: Guo F, Cao Z, Guo H, Li S Abstract The action mechanism of long non-coding ribonucleic acid-homeobox transcript antisense ribonucleic acid (lncRNA-HOTAIR) in the regulation of the Wnt signaling pathway on the drug resistance of non-small cell lung cancer was investigated. Forty eight patients with non-small cell lung cancer, who were treated with cisplatin (DDP) as neoadjuvant chemotherapy, were selected from the specimen bank of the Department of Pathology of Peking Union Medical College Hospital. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the messenger RNA (mRNA) level of...
Source: Experimental and Therapeutic Medicine - May 30, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

TRAP1 Regulates Autophagy in Lung Cancer Cells
Conclusions: Our results show that even though TRAP1 depletion affects both normal MRC‐5 and tumor A549 cell proliferation, inhibition of autophagy per se led to a decrease in tumor cell mass, while having a reduced effect on the normal cell line. The strategy of targeting TRAP1 in NSCLC shows future potential therapeutic applications. This article is protected by copyright. All rights reserved.
Source: European Journal of Clinical Investigation - January 31, 2018 Category: General Medicine Authors: Ines A. Barbosa, Ignacio Vega ‐Naredo, Rute Loureiro, Ana F. Branco, Rita Garcia, Patricia M. Scott, Paulo J. Oliveira Tags: Original Article Source Type: research

MUC1 downregulation inhibits non-small cell lung cancer progression in human cell lines.
In conclusion, the findings indicated that silencing of MUC1 gene may inhibit the development of NSCLC cells. PMID: 29104655 [PubMed]
Source: Experimental and Therapeutic Medicine - November 7, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research

Identification of microRNAs involved in gefitinib resistance of non-small-cell lung cancer through the insulin-like growth factor receptor 1 signaling pathway.
Authors: Ma W, Kang Y, Ning L, Tan J, Wang H, Ying Y Abstract Multiple clinical and experimental studies have suggested that epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) may be effective at treating advanced non-small cell lung cancer (NSCLC), however, the molecular basis of primary resistance to EGFR-TKIs in NSCLC remains unclear. In the current study, the insulin-like growth factor 1 receptor (IGF-1R) gene in the gefitinib-resistant human lung adenocarcinoma epithelial cell line A549 (A549/GR) was silenced using small interfering RNA (siRNA) in order to determine the role of microRNA (m...
Source: Experimental and Therapeutic Medicine - September 17, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research

CD44 silencing decreases the expression of stem cell-related factors induced by transforming growth factor β1 and tumor necrosis factor α in lung cancer: Preliminary findings.
Authors: Nurwidya F, Takahashi F, Kato M, Baskoro H, Hidayat M, Wirawan A, Takahashi K Abstract The mechanism underlying increased concentrations of cancer stem cells (CSCs)-associated factors in non-small cell lung cancer (NSCLC) cells treated with transforming growth factor β1 (TGFβ1) and tumor necrosis factor α (TNFα), is still not clear. The purpose of this study was to investigate the possible role of CD44 in the regulation of CSC-associated genes, by analyzing the effect of CD44 knockdown on the expression of CSC-associated genes. A549, a NSCLC cell line that expresses CD44 antigen, was treated with TGFβ...
Source: Bosnian Journal of Basic Medical Sciences - April 29, 2017 Category: Journals (General) Tags: Bosn J Basic Med Sci Source Type: research

Over-expression of small ubiquitin-like modifier proteases 1 predicts chemo-sensitivity and poor survival in non-small cell lung cancer.
CONCLUSIONS: SENP1 may be a promising predictor of survival, a predictive factor of chemo-sensitivity for NSCLC patients, and potentially a desirable drug target for lung carcinoma target therapy. PMID: 25430449 [PubMed - in process]
Source: Chinese Medical Journal - December 1, 2014 Category: Journals (General) Authors: Mu J, Zuo Y, Yang W, Chen Z, Liu Z, Tu J, Li Y, Yuan Z, Cheng J, He J Tags: Chin Med J (Engl) Source Type: research

SLUG is activated by nuclear factor kappa B and confers human alveolar epithelial A549 cells resistance to tumor necrosis factor-alpha-induced apoptosis
Conclusions: The findings suggested that TNF-alpha treatment promoted apoptosis via the NF-kappaB-dependent PUMA pathway. The anti-apoptotic role of TNF-alpha was via NF-kappaB-dependent SLUG and Bcl-2 pathway at a later time.
Source: BioMed Central - January 22, 2013 Category: Journals (General) Authors: Yaopeng WangBin YueXuyi YuZhan WangMingzhao Wang Source Type: research