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Isoproterenol-induced hypertrophy of neonatal cardiac myocytes and H9c2 cell is dependent on TRPC3-regulated CaV1.2 expression.
In this study, we aimed to examine the relationship between these two proteins and characterize their role in neonatal cardiomyocytes. We measured CaV1.2 expression in the hearts of wild-type (WT) and Trpc3-/- mice, and examined the effects of Trpc3 knockdown and overexpression in the rat cell line H9c2. We also compared the hypertrophic responses of neonatal cardiomyocytes cultured from Trpc3-/- mice to a representative hypertrophy-causing drug, isoproterenol (ISO), and measured the activity of nuclear factor of activated T cells 3 (NFAT3) in neonatal cardiomyocytes (NCMCs). We inhibited the L-type current with nifedipine...
Source: Cell Calcium - October 6, 2020 Category: Cytology Authors: Han JW, Kang C, Kim Y, Lee MG, Kim JY Tags: Cell Calcium Source Type: research

Upregulation of Airway Smooth Muscle Calcium-Sensing Receptor by Low Molecular Weight Hyaluronan.
Abstract We investigated the mechanisms involved in the development of airway hyperresponsiveness (AHR) in mice following exposure to halogens. Male mice (C57BL/6; 20-25 g) exposed to either bromine (Br2) or Cl2 (600 or 400 ppm, respectively, for 30 min) developed AHR 24 hours post exposure. Nifedipine (an L-type calcium channel blocker) administered subcutaneously post Br2 or Cl2 exposure (5 mg/kg BW), resulted in higher AHR compared to Br2 or Cl2 alone. In contrast, diltiazem (a non-dihydropyridine L-type calcium channel blocker) decreased AHR to control (air) values. Exposure of immortalized human airway smooth...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 7, 2020 Category: Cytology Authors: Lazrak A, Yu Z, Doran S, Jian M, Creighton J, Laube M, Garantziotis S, Prakash YS, Matalon S Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Cyclic adenosine monophosphate-dependent activation of transient receptor potential vanilloid 4 (TRPV4) channels in osteoblast-like MG-63 cells.
Abstract Parathyroid hormone (PTH) directly interacts with bone remodeling osteoblasts and osteocytes expressing the G-protein coupled receptor PTH receptor 1 (PTH1R), and its osteoanabolic effects mostly involve the cAMP/PKA signaling cascade. Considering that PTH-dependent calcium entry in rat enterocytes is reproduced by the adenylate cyclase agonist forskolin or by cAMP analogues, possible involvement of calcium as a second messenger in PTH-dependent cAMP signaling was investigated in MG-63 cells. First, Ca2+ influx was confirmed in Fluo3-loaded MG-63 cells treated with a cell-permeable cAMP analog. Second, PT...
Source: Cellular Signalling - November 24, 2019 Category: Cytology Authors: Perez AP, Regnier M, Lizotte J, Martineau C, Scorza T, Moreau R Tags: Cell Signal Source Type: research

Overexpression of Cardiac-Specific Kinase TNNI3K Promotes Mouse Embryonic Stem Cells Differentiation into Cardiomyocytes
Conclusion: our study indicated that TNNI3K overexpression promoted mESC differentiating into beating cardiomyocytes and induced up-regulating expression of cTnT by PKC ε signal pathway, which suggested a modulation of TNNI3K activity as a potential therapeutic approach for ischemic cardiac disease.Cell Physiol Biochem 2017;41:381 –398
Source: Cellular Physiology and Biochemistry - January 27, 2017 Category: Cytology Source Type: research

Chronic fluoxetine administration increases expression of the L-channel gene Cav1.2 in astrocytes from the brain of treated mice and in culture and augments K(+)-induced increase in Ca(2+)i.
Abstract We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-mediated stimulation of the ryanodine receptor (RyR) the increase in free cytosolic Ca(2+) [Ca(2+)]i is substantial. The other Cav1 subtype expressed in brain is Cav1.2, which is even expressed in higher density. Although the different primers used for the two genes preclude exact quantitative co...
Source: Cell Calcium - January 22, 2014 Category: Cytology Authors: Du T, Liang C, Li B, Hertz L, Peng L Tags: Cell Calcium Source Type: research

Inhibition of L-type Ca(2+) channels by curcumin requires a novel protein kinase-theta isoform in rat hippocampal neurons.
In this study, we report that curcumin selectively inhibits L-type Ca(2+) channel currents in cultured rat hippocampal neurons. Whole-cell currents were recorded using 10mM barium as a charge carrier. Curcumin reversibly inhibited high-voltage-gated Ca(2+) channel (HVGCC) currents (I(Ba)) in a concentration-dependent manner but had no apparent effects on the cells treated with nifedipine, a specific L-type Ca(2+) channel blocker. Curcumin did not markedly affect the activation of L-type Ca(2+) channels while significantly shifted the inactivation curve in the hyperpolarizing direction. Pretreatment of cells with the classi...
Source: Cell Calcium - December 19, 2012 Category: Cytology Authors: Liu K, Gui B, Sun Y, Shi N, Gu Z, Zhang T, Sun X Tags: Cell Calcium Source Type: research