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Drug: Nifedipine

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Total 15 results found since Jan 2013.

Isoproterenol-induced hypertrophy of neonatal cardiac myocytes and H9c2 cell is dependent on TRPC3-regulated CaV1.2 expression.
In this study, we aimed to examine the relationship between these two proteins and characterize their role in neonatal cardiomyocytes. We measured CaV1.2 expression in the hearts of wild-type (WT) and Trpc3-/- mice, and examined the effects of Trpc3 knockdown and overexpression in the rat cell line H9c2. We also compared the hypertrophic responses of neonatal cardiomyocytes cultured from Trpc3-/- mice to a representative hypertrophy-causing drug, isoproterenol (ISO), and measured the activity of nuclear factor of activated T cells 3 (NFAT3) in neonatal cardiomyocytes (NCMCs). We inhibited the L-type current with nifedipine...
Source: Cell Calcium - October 6, 2020 Category: Cytology Authors: Han JW, Kang C, Kim Y, Lee MG, Kim JY Tags: Cell Calcium Source Type: research

Upregulation of Airway Smooth Muscle Calcium-Sensing Receptor by Low Molecular Weight Hyaluronan.
Abstract We investigated the mechanisms involved in the development of airway hyperresponsiveness (AHR) in mice following exposure to halogens. Male mice (C57BL/6; 20-25 g) exposed to either bromine (Br2) or Cl2 (600 or 400 ppm, respectively, for 30 min) developed AHR 24 hours post exposure. Nifedipine (an L-type calcium channel blocker) administered subcutaneously post Br2 or Cl2 exposure (5 mg/kg BW), resulted in higher AHR compared to Br2 or Cl2 alone. In contrast, diltiazem (a non-dihydropyridine L-type calcium channel blocker) decreased AHR to control (air) values. Exposure of immortalized human airway smooth...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 7, 2020 Category: Cytology Authors: Lazrak A, Yu Z, Doran S, Jian M, Creighton J, Laube M, Garantziotis S, Prakash YS, Matalon S Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Cyclic adenosine monophosphate-dependent activation of transient receptor potential vanilloid 4 (TRPV4) channels in osteoblast-like MG-63 cells.
Abstract Parathyroid hormone (PTH) directly interacts with bone remodeling osteoblasts and osteocytes expressing the G-protein coupled receptor PTH receptor 1 (PTH1R), and its osteoanabolic effects mostly involve the cAMP/PKA signaling cascade. Considering that PTH-dependent calcium entry in rat enterocytes is reproduced by the adenylate cyclase agonist forskolin or by cAMP analogues, possible involvement of calcium as a second messenger in PTH-dependent cAMP signaling was investigated in MG-63 cells. First, Ca2+ influx was confirmed in Fluo3-loaded MG-63 cells treated with a cell-permeable cAMP analog. Second, PT...
Source: Cellular Signalling - November 24, 2019 Category: Cytology Authors: Perez AP, Regnier M, Lizotte J, Martineau C, Scorza T, Moreau R Tags: Cell Signal Source Type: research

Investigation of morin-induced insulin secretion in cultured pancreatic cells.
This article is protected by copyright. All rights reserved. PMID: 28699234 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Pharmacology and Physiology - July 12, 2017 Category: Drugs & Pharmacology Authors: Lin MH, Hsu CC, Lin J, Cheng JT, Wu MC Tags: Clin Exp Pharmacol Physiol Source Type: research

Overexpression of Cardiac-Specific Kinase TNNI3K Promotes Mouse Embryonic Stem Cells Differentiation into Cardiomyocytes
Conclusion: our study indicated that TNNI3K overexpression promoted mESC differentiating into beating cardiomyocytes and induced up-regulating expression of cTnT by PKC ε signal pathway, which suggested a modulation of TNNI3K activity as a potential therapeutic approach for ischemic cardiac disease.Cell Physiol Biochem 2017;41:381 –398
Source: Cellular Physiology and Biochemistry - January 27, 2017 Category: Cytology Source Type: research

Regulation of BMP2‐induced intracellular calcium increases in osteoblasts
In this study, intracellular Ca2+ concentration ([Ca2+]i) upon BMP2 application, focal adhesion kinase (FAK) and Src activities were measured in the MC3T3‐E1 osteoblast cell line using fluorescence resonance energy transfer‐based biosensors. Increase in [Ca2+]i, FAK, and Src activities were observed during BMP2 stimulation. The removal of extracellular calcium, the application of membrane channel inhibitors streptomycin or nifedipine, the FAK inhibitor PF‐573228 (PF228), and the alkaline phosphatase (ALP) siRNA all blocked the BMP2‐stimulated [Ca2+]i increase, while the Src inhibitor PP1 did not. In contrast, a gen...
Source: Journal of Orthopaedic Research - March 4, 2016 Category: Orthopaedics Authors: Wenfeng Xu, Bo Liu, Xue Liu, Martin Y. M. Chiang, Bo Li, Zichen Xu, Xiaoling Liao Tags: Research Article Source Type: research

The regulation of BMP2‐induced intracellular calcium increases in osteoblasts
This article is protected by copyright. All rights reserved
Source: Journal of Orthopaedic Research - February 1, 2016 Category: Orthopaedics Authors: Wenfeng Xu, Bo Liu, Xue Liu, Martin Chiang, Bo Li, Zichen Xu, Xiaoling Liao Tags: Research Article Source Type: research

AMP-activated protein kinase is involved in perfluorohexanesulfonate -induced apoptosis of neuronal cells.
Abstract Perfluorohexanesulfonate (PFHxS), one of the major perfluoroalkyl compounds (PFCs), has been used in a variety of industrial and consumer applications and detected in serum in the general population. This raised a concern over its possible detrimental health effects, including neurotoxic effects. We have previously shown that PFHxS induced neuronal apoptosis via the NMDA receptor-mediated extracellular signal-regulated kinase (ERK) pathway. Recently, it has been reported that AMP-activated protein kinase (AMPK) acts as a key signal molecule in neuronal excitotoxicity as well as providing a neuroprotective...
Source: Chemosphere - January 27, 2016 Category: Chemistry Authors: Lee YJ, Choi SY, Yang JH Tags: Chemosphere Source Type: research

Voltage-gated calcium channel blockers deregulate macroautophagy in cardiomyocytes.
VOLTAGE-GATED CALCIUM CHANNEL BLOCKERS DEREGULATE MACROAUTOPHAGY IN CARDIOMYOCYTES. Int J Biochem Cell Biol. 2015 Sep 28; Authors: Pushparaj C, Das A, Purroy R, Nàger M, Herreros J, Pamplona R, Cantí C Abstract Voltage-gated calcium channel blockers are widely used for the management of cardiovascular diseases, however little is known about their effects on cardiac cells in vitro. We challenged neonatal ventricular cardiomyocytes (CMs) with therapeutic L-type and T-type Ca(2+) channel blockers (nifedipine and mibefradil, respectively), and measured their effects on cell stress and survival, using fl...
Source: The International Journal of Biochemistry and Cell Biology - September 28, 2015 Category: Biochemistry Authors: Pushparaj C, Das A, Purroy R, Nàger M, Herreros J, Pamplona R, Cantí C Tags: Int J Biochem Cell Biol Source Type: research

Homer proteins mediate the interaction between STIM1 and Cav1.2 channels
Publication date: Available online 21 February 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Natalia Dionisio , Tarik Smani , Geoffrey E. Woodard , Antonio Castellano , Gines M. Salido , Juan A. Rosado STIM1 is an ubiquitous Ca2+ sensor of the intracellular, agonist-sensitive, Ca2+ stores that communicates the filling state of the Ca2+ compartments to plasma membrane store-operated Ca2+ (SOC) channels. STIM1 has been presented as a point of convergence between store-operated and voltage-operated Ca2+ influx, both inducing activation of SOC channels while suppressing Cav1.2 channels....
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 22, 2015 Category: Molecular Biology Source Type: research

Nifedipine inhibits ox-LDL-induced lipid accumulation in human blood-derived macrophages.
In this study, we examined the effects of nifedipine on some ox-LDL-associated changes in human blood-derived macrophages. We isolated monocytes from normal human blood and differentiated them into macrophages. We then treated these human macrophages with ox-LDL and/or nifedipine, and examined lipid accumulation and expression levels of two scavenge receptors CD36 and SR-A as well as a protein kinase PKC-θ. Nifedipine treatment substantially reduced lipid accumulation and the expression of CD36, SR-A, and protein kinase C (PKC)-θ in human macrophages treated with ox-LDL. Silencing of PKC-θ using siRNA also reduced the e...
Source: Biochemical and Biophysical Research communications - January 12, 2015 Category: Biochemistry Authors: Zhang Q, Sha Ma AZ, Wang C, Tang WQ, Song ZY Tags: Biochem Biophys Res Commun Source Type: research

Chronic fluoxetine administration increases expression of the L-channel gene Cav1.2 in astrocytes from the brain of treated mice and in culture and augments K(+)-induced increase in Ca(2+)i.
Abstract We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-mediated stimulation of the ryanodine receptor (RyR) the increase in free cytosolic Ca(2+) [Ca(2+)]i is substantial. The other Cav1 subtype expressed in brain is Cav1.2, which is even expressed in higher density. Although the different primers used for the two genes preclude exact quantitative co...
Source: Cell Calcium - January 22, 2014 Category: Cytology Authors: Du T, Liang C, Li B, Hertz L, Peng L Tags: Cell Calcium Source Type: research

Dihydropyridine Ca2+ Channel Blockers Increase Cytosolic Ca2+ by Activating Ca2+-sensing Receptors in Pulmonary Arterial Smooth Muscle Cells.
Conclusions: The dihydropyridine derivatives increase [Ca(2+)](cyt) by potentiating the activity of CaSR in PASMC independently of their blocking (or activating) effect on Ca(2+) channels; therefore, it is possible that use the dihydropyridine Ca(2+) channel blockers (e.g., nifedipine) to treat IPAH patients with upregulated CaSR in PASMC may exacerbate pulmonary hypertension. PMID: 23300272 [PubMed - as supplied by publisher]
Source: Circulation Research - January 8, 2013 Category: Cardiology Authors: Yamamura A, Yamamura H, Guo Q, Zimnicka AM, Wang J, Ko EA, Smith KA, Pohl NM, Song S, Zeifman A, Makino A, Yuan JX Tags: Circ Res Source Type: research

Inhibition of L-type Ca(2+) channels by curcumin requires a novel protein kinase-theta isoform in rat hippocampal neurons.
In this study, we report that curcumin selectively inhibits L-type Ca(2+) channel currents in cultured rat hippocampal neurons. Whole-cell currents were recorded using 10mM barium as a charge carrier. Curcumin reversibly inhibited high-voltage-gated Ca(2+) channel (HVGCC) currents (I(Ba)) in a concentration-dependent manner but had no apparent effects on the cells treated with nifedipine, a specific L-type Ca(2+) channel blocker. Curcumin did not markedly affect the activation of L-type Ca(2+) channels while significantly shifted the inactivation curve in the hyperpolarizing direction. Pretreatment of cells with the classi...
Source: Cell Calcium - December 19, 2012 Category: Cytology Authors: Liu K, Gui B, Sun Y, Shi N, Gu Z, Zhang T, Sun X Tags: Cell Calcium Source Type: research

Electrical field stimulation induces cardiac fibroblast proliferation through the calcineurin-NFAT pathway.
Abstract Most cardiac diseases are associated with fibrosis. Calcineurin (CaN) is regulated by Ca(2+)/calmodulin (CaM). The CaN-NFAT (nuclear factor of activated T cell) pathway is involved in the process of cardiac diseases, such as cardiac hypertrophy, but its effect on myocardial fibrosis remains unclear. The present study investigates whether the CaN-NFAT pathway is involved in cardiac fibroblast (CF) proliferation induced by electrical field stimulation (EFS), which recently became a popular treatment for heart failure and cardiac tissue engineering. CF proliferation was evaluated by a cell survival assay (MT...
Source: Canadian Journal of Physiology and Pharmacology - December 1, 2012 Category: Drugs & Pharmacology Authors: Chen QQ, Zhang W, Chen XF, Bao YJ, Wang J, Zhu WZ Tags: Can J Physiol Pharmacol Source Type: research