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Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models
Neural Plast. 2022 Feb 21;2022:3172861. doi: 10.1155/2022/3172861. eCollection 2022.ABSTRACTRecently, we showed that DNA double-strand breaks (DSBs) are increased by the Aβ 42-amyloid peptide and decreased by all-trans retinoic acid (RA) in SH-SY5Y cells and C57BL/6J mice. The present work was aimed at investigating DSBs in cells and murine models of Alzheimer's disease carrying the preseniline-1 (PS1) P117L mutation. We observed that DSBs could hardly decrease following RA treatment in the mutated cells compared to the wild-type cells. The activation of the amyloidogenic pathway is proposed in the former case as Aβ 42- ...
Source: Neural Plasticity - March 3, 2022 Category: Neurology Authors: Marcella M Authiat Emmanuelle Gruz-Gibelli Julien Colas Estelle Bianchi Marta Garcia-Arauzo Pascale Marin Fran çois R Herrmann Armand Savioz Source Type: research

The small molecule inhibitor PR-619 protects retinal ganglion cells against glutamate excitotoxicity
This study was designed to investigate the role of PR-619 in regulating mitophagy of RGCs under glutamate excitotoxicity. Primary cultured RGCs were incubated with PR-619 or vehicle control in the excitotoxicity model of 100 µM glutamate treatment. Mitochondrial membrane potential was assessed by JC-1 assay. Cytotoxicity of RGCs was measured by LDH activity. Proteins levels of parkin, optineurin, LAMP1, Bax, Bcl-2 and the LC3-II/I ratio were analyzed by western blot. The distribution and morphology of mitochondria in RGCs was stained by MitoTracker and antibody against mitochondria membrane protein, and examined by confoc...
Source: NeuroReport - October 6, 2020 Category: Neurology Tags: Degeneration and Repair Source Type: research

bFGF promotes neurological recovery from neonatal hypoxic –ischemic encephalopathy by IL‐1β signaling pathway‐mediated axon regeneration
ConclusionsWe showed that IL ‐1β‐mediated axon regeneration underlie the mechanism of bFGF for the treatment of HIBD in neonatal rats. Results from this study would provide insights and molecular basis for future therapeutics in treating HIBD.
Source: Brain and Behavior - June 10, 2020 Category: Neurology Authors: Zheng Ma, Fang Wang, Lu ‐Lu Xue, Ying‐Jie Niu, Yue Hu, Zhang‐Yu Su, Jin Huang, Rui‐Ze Niu, Ting‐Hua Wang, Ying‐Chun Ba, Liu‐Lin Xiong, Xue Bai Tags: ORIGINAL RESEARCH Source Type: research

Steroid receptor RNA activator affects the development of poststroke depression by regulating the peroxisome proliferator-activated receptor γ signaling pathway
The long noncoding RNA, steroid receptor RNA activator (SRA), has been reported to be involved in the development of many types of disease in humans. The aim of this study was to evaluate whether SRA was associated with poststroke depression (PSD). A PSD rat model was established, and depression-like behaviors and sucrose consumption in rats with PSD were analyzed. Reverse transcription-quantitative PCR (RT-PCR), western blot and luciferase dual reporter assay analyses were performed to detect the expression of peroxisome proliferator-activated receptor γ (PPARγ) expression following SRA small interfering RNA (siRNA) tre...
Source: NeuroReport - December 5, 2019 Category: Neurology Tags: Degeneration and Repair Source Type: research

Identification of the biological affection of long noncoding RNA BC200 in Alzheimer’s disease
BC200 is a long noncoding RNA expressed at high levels in the Alzheimer’s disease (AD), and blocking of BC200 by siRNA is assumed to be an effective method for various disease therapy. We have established an AD cell model overexpressing amyloid β-peptide (Aβ)1-42 to observe the effects of BC200 on the cell viability and apoptosis, and to investigate the associated underlying mechanisms. Efficient knockdown and overexpression of BC200 were established using BC200 siRNA and BC200 mimics, respectively. Cell viability following BC200 knockdown and overexpression was assessed by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenylte...
Source: NeuroReport - August 3, 2018 Category: Neurology Tags: Degeneration and Repair Source Type: research

Translesion Synthesis DNA Polymerase Kappa Is Indispensable for DNA Repair Synthesis in Cisplatin Exposed Dorsal Root Ganglion Neurons
AbstractIn the peripheral nervous system (PNS) in the absence of tight blood barrier, neurons are at increased risk of DNA damage, yet the question of how effectively PNS neurons manage DNA damage remains largely unanswered. Genotoxins in systemic circulation include chemotherapeutic drugs that reach peripheral neurons and damage their DNA. Because neurotoxicity of platinum-based class of chemotherapeutic drugs has been implicated in PNS neuropathies, we utilized an in vitro model of Dorsal Root Ganglia (DRGs) to investigate how peripheral neurons respond to cisplatin that forms intra- and interstrand crosslinks with their...
Source: Molecular Neurobiology - April 8, 2017 Category: Neurology Source Type: research

C-Terminal Binding Protein is Involved in Promoting to the Carcinogenesis of Human Glioma
AbstractC-terminal binding protein (CtBP) is responsible for regulating the pathogenesis of a lot of cancer types. However, whether CtBP1/2 is involved in regulating the growth and development of human glioma is still obscure. In the present study presented here, our results firstly reveal that CtBP1/2 deficiency, induced by siRNA interference, disrupts the functional integrity of the MRN complex that is responsible for DNA repair in human glioma cells. The dysfunction of the MRN complex further contributes to the up-regulation of ATM and Rad3-related kinase (ATR) and Chk1 signaling pathway, which inhibits cell cycle progr...
Source: Molecular Neurobiology - October 2, 2016 Category: Neurology Source Type: research

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