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Efficient siRNA-peptide conjugation for specific targeted delivery into tumor cells
Chem. Commun., 2017, Advance Article DOI: 10.1039/C6CC10287E, CommunicationAlbert Gandioso, Anna Massaguer, Nuria Villegas, Candida Salvans, Dani Sanchez, Isabelle Brun-Heath, Vicente Marchan, Modesto Orozco, Montserrat Terrazas Efficient click siRNA-peptide conjugation for targeted delivery into tumor cells. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - February 20, 2017 Category: Chemistry Authors: Albert Gandioso Source Type: research
Oligomeric S100A4 Is Associated With Monocyte Innate Immune Memory and Bypass of Tolerance to Subsequent Stimulation With Lipopolysaccharides
Conclusion: Bypass of tolerance by DAMPs might be a phenomenon as important as TI, since it could explain how chronic inflammation can be maintained in spite of an environment with multiple TLR2/TLR4-ligands. In RA monocytes, a PRDM8-dependent TI mechanism could be responsible for sustained chemokine/cytokines levels.
Introduction
Monocytes and macrophages play a central role in the pathophysiology of inflammation. For instance, in rheumatoid arthritis (RA), activated monocytes massively infiltrate synovial tissues and produce tumor necrosis factor-α (TNFα) (1–3). Accordingly, therapies aime...
Source: Frontiers in Immunology - April 14, 2019 Category: Allergy & Immunology Source Type: research
Silenced suppressor of cytokine signaling 1 (SOCS1) enhances the maturation and antifungal immunity of dendritic cells in response to Candida albicans in vitro
In conclusion, our findings support the view that SOCS1 protein is a critical inhibitory molecule for controlling cytokine response and antigen presentation by DCs, thereby regulating the magnitude of innate and adaptive immunities by generating IFN-γ-production T cells (Th1)—but not Th17—from naïve CD4+ T cells. Our study demonstrates that SOCS1 siRNA can serve as a useful vehicle to modulate the function of DCs against C. albicans infection.
Source: Immunologic Research - November 9, 2014 Category: Allergy & Immunology Source Type: research
Silencing SOCS1 in dendritic cells promote survival of mice with systemic Candida albicans infection via inducing Th1-cell differentiation.
Abstract
Enhancing the immunity conferred by dendritic cells (DCs) to fungal infection represents a promising strategy in the number of immunocompromised individuals. In a previous study, we demonstrated that suppressor of cytokine signaling 1 (SOCS1) silencing can promote the maturation of DCs and induce an immune response against Candida albicans (C. albicans) in vitro. Herein, the effectiveness of SOCS1 suppression administered by SOCS1-siRNA-treated DCs is further evaluated in systemic candidiasis mouse model. The SOCS1-silenced DCs increase mouse survival and significantly decrease fungal colonization in the ...
Source: Immunology Letters - March 23, 2018 Category: Allergy & Immunology Authors: Shi D, Li D, Wang Q, Kong X, Mei H, Shen Y, Liu W Tags: Immunol Lett Source Type: research
ATF2, {beta}-Glucans, and IL-23 Signal Transduction
Current views on the control of IL-23 production focus on the regulation of il23a, the gene encoding IL-23 p19, by NF-κB in combination with other transcription factors. C/EBP homologous protein (CHOP), X2-Box-binding protein 1 (XBP1), activator protein 1 (AP1), SMAD, CCAAT/enhancer-binding protein (C/EBPβ), and cAMP-response element-binding protein (CREB) have been involved in response to LPS, but no data are available regarding the mechanism triggered by the fungal mimic and β-glucan-containing stimulus zymosan, which produces IL-23 and to a low extent the related cytokine IL-12 p70. Zymosan induced the mobilization o...
Source: Journal of Biological Chemistry - August 14, 2014 Category: Chemistry Authors: Rodriguez, M., Domingo, E., Alonso, S., Frade, J. G., Eiros, J., Crespo, M. S., Fernandez, N. Tags: Immunology Source Type: research
Rab14 Regulates Maturation of Macrophage Phagosomes Containing the Fungal Pathogen Candida albicans and Outcome of the Host-Pathogen Interaction Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Avoidance of innate immune defense is an important mechanism contributing to the pathogenicity of microorganisms. The fungal pathogen Candida albicans undergoes morphogenetic switching from the yeast to the filamentous hyphal form following phagocytosis by macrophages, facilitating its escape from the phagosome, which can result in host cell lysis. We show that the intracellular host trafficking GTPase Rab14 plays an important role in protecting macrophages from lysis mediated by C. albicans hyphae. Live-cell imaging of macrophages expressing green fluorescent protein (GFP)-tagged Rab14 or dominant negative Rab14, or with ...
Source: Infection and Immunity - March 17, 2015 Category: Infectious Diseases Authors: Okai, B., Lyall, N., Gow, N. A. R., Bain, J. M., Erwig, L.-P. Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
New signaling pathways govern the host response to C. albicans infection in various niches RESEARCH
Candida albicans, the major invasive fungal pathogen of humans, can cause both debilitating mucosal infections and fatal invasive infections. Understanding the complex nature of the host–pathogen interaction in each of these contexts is essential to developing desperately needed therapies to treat fungal infections. RNA-seq enables a systems-level understanding of infection by facilitating comprehensive analysis of transcriptomes from multiple species (e.g., host and pathogen) simultaneously. We used RNA-seq to characterize the transcriptomes of both C. albicans and human endothelial cells or oral epithelial cells du...
Source: Genome Research - April 9, 2015 Category: Genetics & Stem Cells Authors: Liu, Y., Shetty, A. C., Schwartz, J. A., Bradford, L. L., Xu, W., Phan, Q. T., Kumari, P., Mahurkar, A., Mitchell, A. P., Ravel, J., Fraser, C. M., Filler, S. G., Bruno, V. M. Tags: RESEARCH Source Type: research
Secreted aspartic protease 2 of Candida albicans inactivates factor H and the macrophage factor H-receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18).
In this study, we characterized FH binding to human monocyte-derived macrophages. Inhibition studies with antibodies and siRNA targeting CR3 (CD11b/CD18) and CR4 (CD11c/CD18), as well as analysis of colocalization of FH with these integrins indicated that both function as FH receptors on macrophages. Preincubation of C. albicans yeast cells with FH induced increased production of IL-1β and IL-6 in macrophages. Furthermore, FH enhanced zymosan-induced production of these cytokines. C. albicans Sap2 cleaved FH, diminishing its complement regulatory activity, and Sap2-treatment resulted in less detectable CR3 and CR4 on macr...
Source: Immunology Letters - August 22, 2015 Category: Allergy & Immunology Authors: Svoboda E, Schneider AE, Sándor N, Lermann U, Staib P, Kremlitzka M, Bajtay Z, Barz D, Erdei A, Józsi M Tags: Immunol Lett Source Type: research
Chitinase 3-Like 1 Promotes Candida albicans Killing and Preserves Corneal Structure and Function by Controlling Host Antifungal Responses Fungal and Parasitic Infections
This study sought to elucidate the role of CHI3L1 in augmenting the corneal innate immune response to Candida albicans infection in an animal model of fungal keratitis. Flagellin applied topically 24 h prior to C. albicans inoculation significantly protected the corneal from C. albicans and induced CHI3L1 expression in C57BL/6 mouse corneas. CHI3L1, however, played a detectable but minor role in flagellin-induced protection. While C. albicans keratitis was more severe in the corneas treated with Chi3l1 small interfering RNA (siRNA), corneas treated with recombinant CHI3L1 before C. albicans inoculation had markedly amelior...
Source: Infection and Immunity - September 10, 2015 Category: Infectious Diseases Authors: Gao, N., Yu, F.-S. X. Tags: Fungal and Parasitic Infections Source Type: research
C.glabrata induced infection of rat tracheal epithelial cells is mediated by TLR-2 induced activation of NF-κB
Publication date: Available online 23 October 2015 Source:Microbial Pathogenesis Author(s): Xue Zhang, Jian Bai, Cheng-Lin Wu, Ye Wu, Ying Zhao, Wen-Ming Song, Xue-Ping Luo An increasing number of reports identified Candida glabrata (C.glabrata) as the important causative agent of invasive pulmonary fungal infection. However, little is known about immune responses to C.glabrata in rat tracheal epithelial cell (RTEC). Here, the effect of C.glabrata on RTEC and the role of TLR-2 and NF-κB in the immune response were investigated by treatment withTLR-2 siRNA and NF-κB inhibitor pyrrolidinedithiocarbamate (PDTC), re...
Source: Microbial Pathogenesis - October 24, 2015 Category: Infectious Diseases Source Type: research
Candida glabrata induced infection of rat tracheal epithelial cells is mediated by TLR-2 induced activation of NF-κB
Publication date: February 2016 Source:Microbial Pathogenesis, Volume 91 Author(s): Xue Zhang, Jian Bai, Cheng-Lin Wu, Ye Wu, Ying Zhao, Wen-Ming Song, Xue-Ping Luo An increasing number of reports identified Candida glabrata (C. glabrata) as the important causative agent of invasive pulmonary fungal infection. However, little is known about immune responses to C. glabrata in rat tracheal epithelial cell (RTEC). Here, the effect of C. glabrata on RTEC and the role of TLR-2 and NF-κB in the immune response were investigated by treatment with TLR-2 siRNA and NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC), re...
Source: Microbial Pathogenesis - December 25, 2015 Category: Infectious Diseases Source Type: research
Efficient siRNA-peptide conjugation for specific targeted delivery to tumor cells
Chem. Commun., 2017, Accepted Manuscript DOI: 10.1039/C6CC10287E, CommunicationAlbert Gandioso, Anna Massaguer, Nuria Villegas, Candida Salvans, Dani Sanchez, Isabelle Brun-Heath, Vicente Marchan, Modesto Orozco, Montserrat Terrazas Despite the broad applicability of the Huisgen cycloaddition reaction, the click functionalization of RNAs with peptides remains still a challenge. Here we describe a straightforward method for the click functionalization... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - February 13, 2017 Category: Chemistry Authors: Albert Gandioso Source Type: research
Candida albicans {beta}-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway Host Response and Inflammation
Oral keratinocytes provide the first line of host defense against oral candidiasis. We speculated that interactions of fungal cell wall components with oral keratinocytes regulate the stress response against Candida infection and examined the expression of genes induced by heat-killed Candida albicans in oral immortalized keratinocytes using a cDNA microarray technique. Of 24,000 genes revealed by that analysis, we focused on HO-1, a stress-inducible gene, as its expression was increased by both heat-killed and live C. albicans. In histological findings, HO-1 expression in the superficial layers of the oral epithelium foll...
Source: Infection and Immunity - March 22, 2018 Category: Infectious Diseases Authors: Ishida, Y., Ohta, K., Naruse, T., Kato, H., Fukui, A., Shigeishi, H., Nishi, H., Tobiume, K., Takechi, M. Tags: Host Response and Inflammation Source Type: research
TLR-2, IL-10 and IL-17-mediated immunity in experimental chemotherapy murine model of systemic candidiasis; cyclophosphamides' impact and roles
In this study, following the establishment of infection and immunosuppression in Balb/c mice model, the mRNA/protein levels of TLR-2, IL-10, IL-17, and Myeloperoxidase (MPO) in serum/kidney were measured using Real-time PCR and ELISA respectively. The survival of mice was checked daily and organ fungal burden was calculated and the histology samples were prepared. Results indicated that the mRNA and protein levels of IL-10, IL-17 and MPO were significantly elevated in immunosuppressed-infected mice (P < 0.05). Conversely, the mRNA level of TLR-2 in this mice were significantly decreased (P < 0.05). We...
Source: Microbial Pathogenesis - May 5, 2018 Category: Infectious Diseases Source Type: research
