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GSE69980 Exclusion of non-expressed genes from the Chikungunya RNAi screen hit identification process
Contributors : Alexander Karlas ; Thomas Meyer ; Hans-Joachim MollenkopfSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo allow exclusion of non-expressed genes from the hit identification process, microarray analyses of (i) Allstars transfected and Chikungunya (CHIKV) infected, (ii) Allstars transfected and non-infected, (iii) non-transfected and CHIKV infected and (iv) non-transfected and non-infected 293 cells were performed. Transfection was done by adding 4x10e5 293 cells (in complete DMEM medium) to a mixture of serum-free RPMI medium, HiperFect (Qiagen) and Allstars siRNAs (Qiagen, final siRNA con...
Source: GEO: Gene Expression Omnibus - May 9, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes
Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided by the dsRBPs Loquacious (Loqs-PB, Loqs-PD) and R2D2, respectively. However, this functional specialization has not been investigated in other dipterans. We were unable to detect Loqs-PD in Ae....
Source: Nucleic Acids Research - April 19, 2015 Category: Research Authors: Haac, M. E., Anderson, M. A. E., Eggleston, H., Myles, K. M., Adelman, Z. N. Tags: Molecular Biology Source Type: research

GSE65070 The hub protein Loqs connects the miRNA and endo-siRNA pathways in mosquitos
Contributors : Mary E Haac ; Michelle A Anderson ; Heaher L Eggleston ; Kevin M Myles ; Zach N AdelmanSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Aedes aegyptiAedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided...
Source: GEO: Gene Expression Omnibus - April 8, 2015 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Aedes aegypti Source Type: research

Inflammasome signaling pathways exert antiviral effect against Chikungunya virus in human dermal fibroblasts.
Abstract Arboviruses represent an emerging threat to human. They are transmitted to vertebrates by the bite of infected arthropods. Early transmission to vertebrates is initiated by skin puncture and deposition of virus in this organ. However, events at the bite site remain largely unknown. Here, we report that Chikungunya virus (CHIKV) and West Nile virus (WNV), despite belonging to distinct viral families, elicit a common antiviral signature in primary human dermal fibroblasts, attesting for the up regulation of interferon signalling pathways and leading to an increased expression of IFN-β, interleukins and che...
Source: Infection, Genetics and Evolution - April 3, 2015 Category: Genetics & Stem Cells Authors: Ekchariyawat P, Hamel R, Bernard E, Wichit S, Surasombatpattana P, Talignani L, Thomas F, Choumet V, Yssel H, Desprès P, Briant L, Missé D Tags: Infect Genet Evol Source Type: research

Proteomics Profiling of Chikungunya-Infected Aedes albopictus C6/36 Cells Reveal Important Mosquito Cell Factors in Virus Replication
In this study, the CHIKV-infected C6/36 cells with differential cellular proteins expression were profiled using two-dimensional gel electrophoresis (2DE) coupled with the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). 2DE analysis on CHIKV-infected C6/36 cells has shown 23 mosquito cellular proteins that are differentially regulated, and which are involved diverse biological pathways, such as protein folding and metabolic processes. Among those identified mosquito proteins, spermatogenesis-associated factor, enolase phosphatase e-1 and chaperonin-60kD have been found to...
Source: PLoS Neglected Tropical Diseases - March 4, 2015 Category: Tropical Medicine Authors: Regina Ching Hua Lee et al. Source Type: research

Proteomic Profiling of Chikungunya Virus-Infected Human Muscle Cells: Reveal The Role of Cytoskeleton Network in CHIKV Replication.
Abstract Chikungunya virus (CHIKV) is an arthropod-borne, positive-sense, single-stranded RNA virus belonging to genus Alphavirus and family Togaviridae. The clinical manifestations developed upon CHIKV-infection include fever, myositis, arthralgia and maculopapular rash. Thus, the re-emergence of CHIKV has posed serious health threats worldwide. Due to the fact that myositis is induced upon CHIKV-infection, we sought to understand the dynamic proteomic regulation in SJCRH30, a human rhabdomyosarcoma cell line, to gain insights on CHIKV pathogenesis. Two-dimensional gel electrophoresis (2DE) in combination of matr...
Source: Journal of Proteomics - June 13, 2014 Category: Biochemistry Authors: Issac TH, Lee TE, Chu JJ Tags: J Proteomics Source Type: research

Chikungunya virus nsP3 & nsP4 interacts with HSP-90 to promote virus replication: HSP-90 inhibitors reduce CHIKV infection and inflammation in vivo.
In this study, using biochemical pull-downs, mass-spectrometry, and microscopic imaging techniques, we have identified novel interactions between CHIKV nsP3 or nsP4 proteins with the host stress-pathway chaperone HSP-90 protein. Indeed, silencing of HSP-90 transcripts using siRNA disrupts CHIKV replication in cultured cells. Furthermore, drugs targeting HSP-90, such as commercially available geldanamycin, as well as other specific HSP-90 inhibitor drugs that had been obtained from a purinome mining approach (HS-10 and SNX-2112) showed dramatic reduction in viral titers and reduced inflammation in a CHIKV mouse model of sev...
Source: Antiviral Research - December 31, 2013 Category: Virology Authors: Rathore AP, Haystead T, Das PK, Merits A, Ng ML, Vasudevan SG Tags: Antiviral Res Source Type: research

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