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Source: Oncology Research

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Total 108 results found since Jan 2013.

SiRNA-Mediated Flotillin-2 (Flot2) Downregulation Inhibits Cell Proliferation, Migration, and Invasion in Gastric Carcinoma Cells.
In conclusion, the present study suggests that the Flot2 protein expression is significantly correlated with cancer progression and poor prognosis in gastric carcinomas, probably due to its role in the regulation of cell proliferation, migration, and invasion in gastric carcinoma cells. PMID: 24854103 [PubMed - in process]
Source: Oncology Research - May 28, 2014 Category: Cancer & Oncology Authors: Cao K, Xie D, Cao P, Zou Q, Lu C, Xiao S, Zhou J, Peng X Tags: Oncol Res Source Type: research

Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth.
Authors: Yin D, Li Y, Guo B, Liu Z, Xu Y, Wang X, Du Y, Xu L, Meng Y, Zhao X, Zhang L Abstract RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG) was successfully synthetized and then used to deliver plasmid-based Stat3 siRNA. The carrier can readily bind plasmid with high transfection efficiency. Moreover, molecular biology studies reveal that Stat3-related gene and pr...
Source: Oncology Research - April 23, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Knockdown of Long Noncoding RNA uc.338 by siRNA Inhibits Cellular Migration and Invasion in Human Lung Cancer Cells.
Authors: Gao X, Gao X, Li C, Zhang Y, Gao L Abstract Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our data showed that the expression of uc.338 in lung cancer was remarkably increased in vivo and in vitro. Depletion of uc.338 with specific siRNA interference retarded the cell proliferative rate in lung cancer cell lines NCI-H929 and H1688. Furthermo...
Source: Oncology Research - October 11, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

siRNA Suppression of NEDD9 Inhibits Proliferation and Enhances Apoptosis in Renal Cell Carcinoma.
In this study, we demonstrate for the first time that NEDD9 was upregulated in RCC tissue and cell lines. Immunohistochemical analysis and quantitative RT-PCR analysis showed low expression of NEDD9 in normal renal tissues and high expression in RCC tissues. In addition, in vitro experiments show that expression of NEDD9 was upregulated in RCC cell lines. Through MTT assay, we observed that NEDD9 knockdown inhibited cell proliferation. Furthermore, flow cytometry analysis showed that NEDD9 downregulation induced apoptosis. Together, our data suggest that abnormal NEDD9 protein expression may be a marker for RCC, and NEDD9 ...
Source: Oncology Research - September 11, 2015 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Ultrasound-mediated vascular endothelial growth factor C (VEGF-C) gene microbubble transfection inhibits growth of MCF-7 breast cancer cells.
Abstract We evaluated the effects of ultrasound-mediated microbubble transfection of VEGF-C siRNA on breast cancer cells in vitro and in vivo. MCF-7 cells were transfected with VEGF-C siRNA and the protein and mRNA expression of VEGF-C was tested using Western blot and qRT-PCR. Twenty nude mice tumors were established by injecting with MCF-7 cells, and were randomized into four groups when palpable tumors reached 190 mm3. The length and width of MCF-7 tumors in mice were measured every 3 days. After 20 days, all mice were killed and the expression of VEGF-C in tumor tissue was also detected by Western blot and qRT...
Source: Oncology Research - July 28, 2013 Category: Cancer & Oncology Authors: Xu Q, Sun T, Tian H, Wang C, Zhou H Tags: Oncol Res Source Type: research

Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells.
Authors: Miao Y, Lu M, Yan Q, Li S, Feng Y Abstract Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP) into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype of PK, has been proposed as a new tumor marker with high expression in various tumor tissues. Here we aimed to explore the effects of siRNA-PKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, in which PKM2 was notably expressed. PKM2 gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected SKOV3 and OVCAR3 cells were evaluated for cell proliferatio...
Source: Oncology Research - March 12, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Silencing of lncRNA-CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo Via Targeting miR-203.
Authors: Wang S, Hui Y, Li X, Jia Q Abstract Gliomas are the most common primary brain tumors with high mortality. The treatment for gliomas is largely limited dueto the uncomprehending pathological mechanism. Here we aimed to investigate the effect of long non-coding RNA (lncRNA) coiled-coil domain-containing 26 (CCDC26) in gliomas progression. In our study, the expression of CCDC26 was found up-regulated in gliomas tissues and cell lines compared with normal tissues and cell lines. Further exploration detected decreased cell proliferation and increased cell apoptosis in U-251 and M059J cells transfected with CCDC...
Source: Oncology Research - June 11, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Overexpression of Glutathione S-transferase P1 Inhibits the Viability and Motility of Prostate Cancer via Targeting MYC and Inactivating the MEK/ERK1/2 Pathways.
Authors: Wang XX, Jia HT, Yang H, Luo MH, Sun T Abstract Prostate cancer (PC) is one of the most common malignancies of men. Glutathione S-transferase P1 (GSTP1) has been suggested to play a protective role in the prostate. The proto-oncogene MYC has been extensively proved to be a key regulator of tumor transformation from early stage to malignant. Our study aims to investigate the mechanism of GSTP1 in the biological behavior of PC. Compared with normal prostate tissues, the expression of GSTP1 was decreased in PC tissues. Conversely, the level of MYC was increased in PC tissues compared with normal tissues. MYC ...
Source: Oncology Research - June 28, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Epigenetic silence of HOTAIR contributes to the metastasis of pancreatic cancer via targeting miR-138.
Authors: Nie H, Zhang Y, Xing R, Li M, Mou Y Abstract Pancreatic cancer (PC) is one of the most common malignant tumors in digestive tract. The expression of HOX transcriptantisense RNA (HOTAIR) is positively correlated with TNM staging of PC. Our study aims to investigate the effect and the related mechanism of HOTAIR on the invasion and migration ability of PC cells. We found that the expression of HOTAIR was significantly increased in PC tissues and four PC cell lines (PANC-1, SW1990, CAPAN-1 aand JF305) compared with normal tissues and human pancreatic HPDE6-c7 cells. HOTAIR was then silenced by transfecting si...
Source: Oncology Research - December 23, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Knockdown of Peripheral Myelin Protein 22 Inhibits the Progression of Chronic Myeloid Leukemia.
Authors: Liu H, Cao HQ, Ta JB, Zhang W, Liu YH Abstract We aimed to explore the underlying mechanism of peripheral myelin protein 22 (PMP22) in the development of chronic myeloid leukemia (CML). The level of PMP22 expression in CD34(+) cells isolated from CML patients' bone marrow samples (BMMCs) and peripheral blood samples (PBMCs) was determined by RT-PCR. In addition, PMP22-siRNA and scrambled control siRNA were transfected into human CML cell line K562 with Lipofectamine 2000 reagent. Cell viability and apoptosis were, respectively, determined by MTT assay and flow cytometry. Besides, the level of caspase 3 and...
Source: Oncology Research - December 5, 2015 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells.
In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OC...
Source: Oncology Research - March 6, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer By Sponging MiR-135a.
This study is aimed to investigate the role of urothelial carcinoma associated 1(UCA1) in the progression of PC. Our results revealed that long non-coding RNAs (lncRNAs)-UCA1 was overexpressed in PC tissues compared with adjacent histologically normal tissues. Down-regulated level of UCA1 was also detected in five human pancreatic cancer cell lines (SW1990，BxPC-3, MiaPaCa-2, PANC-1, CAPAN-1) compared with normal pancreatic duct epithelial HPDE cells. The proliferation of PC cells was inhibited after UCA1 was suppressed by a lentiviral vector. Cell apoptosis rate was largely promoted by down-regulating UCA1. Further resea...
Source: Oncology Research - March 23, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Silencing of NADPH oxidase 4 attenuates hypoxia resistance in neuroblastoma cells SHSY-5Y by inhibiting PI3K/Akt-dependent glycolysis.
Authors: Yu T, Li L, Liu W, Ya B, Cheng H, Xin Q Abstract Hypoxia-induced chemoresistance is a major obstacle in the development of effective cancer therapy. In our study, the reversal abilities of NADPH oxidase 4 (NOX4) silence on the hypoxia resistance and the potential mechanism were investigated. Our data showed that the expression of NOX4 was up-regulated in human neuroblastoma cells SHSY-5Y under hypoxia condition time-dependently. Knockdown of NOX4 expression by siRNA inhibited glycolysis induced by hypoxia through decreasing the expression of glycolysis related proteins (HIF-1α, LDHA, PDK1), decreasing glu...
Source: Oncology Research - February 11, 2018 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Zinc Finger X-Chromosomal Protein (ZFX) Promotes Solid Agar Colony Growth of Osteosarcoma Cells.
Abstract Zinc finger X-chromosomal protein (ZFX) is a member of the zinc finger family of proteins. The importance of ZFX in several cancer types, including prostate cancer, laryngeal squamous cell carcinoma, and glioma, has been addressed. However, the role of ZFX in human osteosarcoma remains unknown. Here we investigated the phenotype of ZFX knockdown on cell proliferation and in vitro tumorigenesis using lentivirus-mediated loss-of-function strategy. The results demonstrated that the proliferation and colony formation ability of human osteosarcoma Saos-2 and MG63 cells was impaired by ZFX small interfering RNA...
Source: Oncology Research - October 23, 2013 Category: Cancer & Oncology Authors: Jiang R, Wang JC, Sun M, Zhang XY, Wu H Tags: Oncol Res Source Type: research

MiR-138 Induces Renal Carcinoma Cell Senescence by Targeting EZH2 and Is Downregulated in Human Clear Cell Renal Cell Carcinoma.
Abstract MiR-138 has been shown to be downregulated in various cancers, including head and neck squamous cell carcinoma (HNSCC) and clear cell renal carcinoma (ccRCC). In the present study, we aimed to reveal the mechanism of miR-138 induction of senescence in renal carcinoma cells and identify its specific target genes. We used qRT-PCR to analyze miR-138 expression levels in renal carcinoma cell lines and ccRCC samples. The activity of β-galactosidase was measured for functional analysis after miR-138 mimic transfection. To identify the targets of miR-138, we used three types of target prediction software to det...
Source: Oncology Research - January 15, 2014 Category: Cancer & Oncology Authors: Liang J, Zhang Y, Jiang G, Liu Z, Xiang W, Chen X, Chen Z, Zhao J Tags: Oncol Res Source Type: research