Silencing of lncRNA-CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo Via Targeting miR-203.

Silencing of lncRNA-CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo Via Targeting miR-203. Oncol Res. 2017 Jun 09;: Authors: Wang S, Hui Y, Li X, Jia Q Abstract Gliomas are the most common primary brain tumors with high mortality. The treatment for gliomas is largely limited dueto the uncomprehending pathological mechanism. Here we aimed to investigate the effect of long non-coding RNA (lncRNA) coiled-coil domain-containing 26 (CCDC26) in gliomas progression. In our study, the expression of CCDC26 was found up-regulated in gliomas tissues and cell lines compared with normal tissues and cell lines. Further exploration detected decreased cell proliferation and increased cell apoptosis in U-251 and M059J cells transfected with CCDC26-siRNA. Besides that, the silence of CCDC26 strongly reduced the would closing rate and the number of invasive cells compared with scramble group. Simultaneously, the expression of miR-203 was found suppressed in gliomas tissues and cells lines. Suppressed level of miR-203 was then elevated in U-251 and M059J cells transfected with CCDC26-siRNA. The result of luciferase activity assay also showed that the luciferase activity was strongly strengthened by adding miR-203 inhibitor into CCDC26 WT group. Moreover, CDCC26-siRNA counteracted the effect of miR-203 inhibitor on facilitating cell viability and mobility in U-251 cells. The in vivo experiment also revealed that CCDC26-siRN...
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research