Epigenetic silence of HOTAIR contributes to the metastasis of pancreatic cancer via targeting miR-138.

Epigenetic silence of HOTAIR contributes to the metastasis of pancreatic cancer via targeting miR-138. Oncol Res. 2017 Dec 21;: Authors: Nie H, Zhang Y, Xing R, Li M, Mou Y Abstract Pancreatic cancer (PC) is one of the most common malignant tumors in digestive tract. The expression of HOX transcriptantisense RNA (HOTAIR) is positively correlated with TNM staging of PC. Our study aims to investigate the effect and the related mechanism of HOTAIR on the invasion and migration ability of PC cells. We found that the expression of HOTAIR was significantly increased in PC tissues and four PC cell lines (PANC-1, SW1990, CAPAN-1 aand JF305) compared with normal tissues and human pancreatic HPDE6-c7 cells. HOTAIR was then silenced by transfecting siRNA into PCNA-1 cells. The decreased invasive cell number with reduced wound closing rate was detected in PANC-1 cells transfected with HOTAIR-siRNA. Besides, microRNA (miR)-138 was predicted and convinced a direct target of HOTAIR. The expression of miR-138 was decreased in PC tissues and cell lines compared with adjacent normal tissues and cells. In addition, the elevated migration, invasion and growth abilities of PC were restrained adding HOTAIR-siRNA in PCNA-1 cells transfected with miR-138 inhibitor. Finally, the in vivo experiment revealed that HOTAIR-siRNA strongly reduced the tumor growth, volume and expression of metastasis-related proteins. Taken together, our research indicated a HOTAIR...
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research