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Knockdown of Wip1 Enhances Sensitivity to Radiation in HeLa Cells Through Activation of p38 MAPK.
The objectives of the study were to investigate the functional role and potential mechanism of wild-type p53-induced phosphatase (Wip1) in cervical cancer cell line HeLa cells, along with the effect of knockdown of Wip1 in combination with γ-irradiation on the HeLa cells. Expression of Wip1 was silenced or overexpressed. After transfection, cell viability was determined. Moreover, γ-irradiation and SB203580 were performed to explore the effect of colony formation and cell apoptosis. Likewise, protein expression levels of p38, p-p38, p53, and p-p53 were assessed in the presence or not of SB203580 and overexpression of Wip...
Source: Oncology Research - September 11, 2015 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Doxorubicin Inhibits Proliferation of Osteosarcoma Cells Through Upregulation of the Notch Signaling Pathway.
Authors: Ji P, Yu L, Guo WC, Mei HJ, Wang XJ, Chen H, Fang S, Yang J Abstract Doxorubicin plays a major role in the treatment of osteosarcoma disorders. The Notch signaling pathway exerts various biological functions, including cell proliferation, differentiation, and apoptosis. In the present study, we investigated the effects of different doses of doxorubicin on proliferation and apoptosis of osteosarcoma cells with or without Notch signaling. Results found that cellular viability was downregulated while caspase 3 activity and expression were promoted in osteosarcoma cells following treatment with various doses o...
Source: Oncology Research - September 11, 2015 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Inhibition of Lung Carcinoma A549 Cell Growth by Knockdown of Hexokinase 2 In Situ and In Vivo.
In this study, we explored the functional role of HK2 in lung cancer cell proliferation and tumorigenesis and determine its expression profile in lung cancer. HK2 expression was increased in primary lung cancer tissues of patients. Knocking down HK2 expression by small interfering RNA (siRNA) inhibited cell proliferation in lung cancer cells and nude mice. Thus, HK2 is required for sustained proliferation and survival of tumor cells in vitro and in vivo, and its aberrant expression may contribute to the pathogenesis of lung cancer. Thus, our study provided evidence that HK2 functions as a novel oncogene in lung cancer and ...
Source: Oncology Research - January 24, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells.
In this study, we explored the mechanism that HSP27 was involved in cisplatin resistance of ovarian cancer both in vitro and clinically. HSP27 protein was found to be upregulated and expressed in cisplatin-resistant ovarian cancer cell line C13*, and HSP27 siRNA transfection reversed the chemoresistance of C13*. We found that HSP27 exerted its chemoresistant role by inhibiting p21 transferring from the nucleus to the plasma through the activation of phosphorylated-Akt pathway. These findings have implications for clinical trials aimed at a potential therapeutic target for ovarian tumors that are refractory to conventional ...
Source: Oncology Research - March 6, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Knockdown of Zinc Transporter ZIP5 by RNA Interference Inhibits Esophageal Cancer Growth In Vivo.
In this study, we found that downregulation of SLC39A5 (ZIP5) by interference resulted in a significant reduction in esophageal cancer tumor volume and weight in vivo. COX2 (cyclooxygenase 2) expression was decreased and E-cadherin expression was increased in the KYSE170K xenografts, which was caused by the downregulation of ZIP5. However, we did not find that the downregulation of ZIP5 caused a change in the relative expressions of cyclin D1, VEGF (vascular endothelial growth factor), MMP9 (matrix metalloprotein 9), and Bcl-2 (B-cell lymphoma/leukmia-2) mRNA or an alteration in the average level of zinc in the peripheral ...
Source: Oncology Research - July 29, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Knockdown of PFTK1 Expression by RNAi Inhibits the Proliferation and Invasion of Human Non-Small Lung Adenocarcinoma Cells.
In this study, we aimed to explore the expression and function of PFTK1 in NSCLC cells. Our results showed that PFTK1 was significantly upregulated in human NSCLC cell lines. Silencing the expression of PFTK1 inhibited the proliferation of NSCLC cells. In addition, silencing the expression of PFTK1 endowed NSCLC cells with decreased migration and invasion abilities, as well as epithelial-mesenchymal transition (EMT) progress in A549 cells. A mechanistic study showed that knockdown of PFTK1 inhibited the expression of β-catenin, cyclin D1, and c-Myc in A549 cells. In summary, we report that small interfering RNA (siRNA)-PF...
Source: Oncology Research - July 29, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

C1qTNF related protein 4 (CTRP4) promotes pancreatic ductal adenocarcinoma cell proliferation and migration via activation of EGFR-PI3K-NF- κB pathway.
In this study, the role of C1qTNF related protein 4 (CTRP4) in the progression of pancreatic ductal adenocarcinoma (PDAC) was explored. We found that, compared with the adjacent non-cancerous tissues and normal pancreatic cells, CTRP4 was markedly upregulated in human PDAC tissues and cell lines. CTRP4 siRNA or pcDNA-CTRP4 expression vectors were transfected into PANC-1 PDAC cells. CTRP4 knockdown sharply reduced IL-8 secretion, causing inactivation of EGFR/PI3K/NF-κB, and suppressing the proliferation and migration of PANC-1 cells. In contrast, CTRP4 overexpression increased IL-8 secretion and EGFR/PI3K/NF-κB activation...
Source: Oncology Research - September 21, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

CLIC1 induces drug resistance in human choriocarcinoma through positive regulation of MRP1.
In conclusion, our results suggest that CLIC1 may serve as a critical mediator of chemoresistance in human choriocarcinoma JeG3 cells. The CLIC1-mediated chemoresistance is achieved through positive regulation of MRP1. Depletion of either CLIC1 or its downstream MRP1 may be a promising therapeutic strategy concerning reversing the chemo-resistance in human choriocarcinoma JeG3 cells. PMID: 27983917 [PubMed - as supplied by publisher]
Source: Oncology Research - December 18, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Gastrin enhances autophagy and promotes gastric carcinoma proliferation via inducing AMPK α.
In conclusion, these findings indicate that gastrin plays a tumorigenic role by promoting autophagy in gastric cancer, and may provide a novel therapeutic target for gastric cancer treatment. PMID: 28059052 [PubMed - as supplied by publisher]
Source: Oncology Research - January 9, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

miR-940 Upregulation Suppresses Cell Proliferation and Induces Apoptosis by Targeting PKC- δ in Ovarian Cancer OVCAR3 Cells.
This study investigated the pivotal role of miR-940 in the progression of ovarian cancer and to reveal the possible molecular mechanism of its action. Ovarian cancer OVCAR3 cells were transfected with the miR-940 vector, miR-940 inhibitor, and/or small interfering RNA (siRNA) targeting PKC-δ (si-PKC-δ), respectively. After transfection, cell viability and cell apoptosis were analyzed, as well as cell proliferation and apoptosis-related protein expression. Compared to the control, miR-940 upregulation suppressed cell viability but induced cell apoptosis. miR-940 upregulation increased the expression of p27, Hes1, survivin...
Source: Oncology Research - January 15, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Inhibition of Beclin-1-Mediated Autophagy by MicroRNA-17-5p Enhanced the Radiosensitivity of Glioma Cells.
Authors: Hou W, Song L, Zhao Y, Liu Q, Zhang S Abstract The role of miRNAs in the radiosensitivity of glioma cells and the underlying mechanism is still far from clear. In the present study, we detected six downregulated and seven upregulated miRNAs in the serum after radiotherapy compared with paired serum samples before radiotherapy via miRNA panel PCR. Among these, miR-17-5p was highly reduced (fold change = -4.21). Further, we validated the levels of miR-17-5p in all serum samples with qRT-PCR. In addition, statistical analysis suggested that a reduced miR-17-5P level was positively associated with advanced...
Source: Oncology Research - January 15, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line SAOS-2 Via Targeting Caspase-8.
In conclusion, overexpression of miR-21 suppressed SAOS-2 cells apoptosis via directly targeting caspase-8. PMID: 28109080 [PubMed - as supplied by publisher]
Source: Oncology Research - January 22, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Knockdown of DDX5 inhibits the proliferation and tumorigenesis in esophageal cancer.
In conclusion, our findings provide the first evidence that siRNA-DDX5 inhibited the proliferation and invasion of EC cells through suppressing the Wnt/β-catenin signaling pathway. Therefore, DDX5 may be a novel potential therapeutic target for the prevention and treatment of EC. PMID: 28244855 [PubMed - as supplied by publisher]
Source: Oncology Research - March 2, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Gamma Irradiation Upregulates B-cell Translocation Gene 2 to Attenuate Cell Proliferation of Lung Cancer Cells Through JNK and NF κB Pathways.
In this study, we aimed to explore the role of γ-ray treatment and correlation with BTG2 in cell proliferation, apoptosis and cell cycle arrest regulation in lung cancer cell line. A549 cell viability, apoptosis rate, and cell cycle were investigated after γ-ray treatment. Then we used siRNA for BTG2 to detect the effect of BTG2 knockdown on the progress of γ-ray treated lung cancer cell. Finally, we investigated the singling pathway that γ-ray might regulate BTG2. We found that γ-ray inhibited A549 cell viability, promoted apoptosis and cell cycle arrest. While the BTG2 knockdown could relieve the effect caused by γ...
Source: Oncology Research - March 3, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

CXCL5 Plays a Promoting Role in Osteosarcoma Cell Migration and Invasion in Autocrine- and Paracrine-Dependent Manners.
Authors: Dang H, Wu W, Wang B, Cui C, Niu J, Chen J, Chen Z, Liu Y Abstract CXCL5, a CXC-type chemokine, is an important attractant for granulocytic immune cells by binding to its receptor CXCR2. Recently, CXCL5/CXCR2 has been found to play an oncogenic role in many human cancers. However, the exact role of CXCL5 in osteosarcoma cell migration and invasion has not been revealed. Here we found that the protein expression of CXCL5 was significantly increased in osteosarcoma tissues compared with that in matched adjacent nontumor tissues. Moreover, the expression of CXCL5 was significantly associated with advanced cli...
Source: Oncology Research - March 10, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

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