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Source: Free Radical Biology and Medicine

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Total 199 results found since Jan 2013.

F2-isoprostanes affect macrophage migration and csf-1 signalling.
F2-ISOPROSTANES AFFECT MACROPHAGE MIGRATION AND CSF-1 SIGNALLING. Free Radic Biol Med. 2018 Aug 07;: Authors: Proudfoot JM, Murrey MW, McLean S, Greenland EL, Barden AE, Croft KD, Galano JM, Durand T, Mori TA, Pixley FJ Abstract F2-isoprostanes (F2-IsoP) are formed in vivo via free radical peroxidation of arachidonic acid. Enhanced oxidative stress is implicated in the development of atherosclerosis in humans and F2-IsoP have been detected in atherosclerotic plaque. Colony stimulating factor-1 (CSF-1) is essential to macrophage survival, proliferation and differentiation and has been detected in human...
Source: Free Radical Biology and Medicine - August 7, 2018 Category: Biology Authors: Proudfoot JM, Murrey MW, McLean S, Greenland EL, Barden AE, Croft KD, Galano JM, Durand T, Mori TA, Pixley FJ Tags: Free Radic Biol Med Source Type: research

Caffeic Acid Attenuates Rat Liver Injury after Transplantation Involving PDIA3-Dependent Regulation of NADPH Oxidase.
This study was undertaken to explore the effect of Caffeic acid (CA), a naturally occurring antioxidant, on I/R injury of grafted liver and the mechanisms involved. Male Sprague-Dawley rats underwent orthotopic liver transplantation (LT) in the absence or presence of CA administration. In vitro, HL7702 cells were subjected to hypoxia/reoxygenation. LT led to apparent hepatic I/R injury, manifested by deteriorated liver function, microcirculatory disturbance and increased apoptosis, along with increased PDIA3 expression and nicotinamide adenosine dinucleotide phosphate (NADPH) oxidase activity, and membrane translocation of...
Source: Free Radical Biology and Medicine - September 12, 2018 Category: Biology Authors: Mu HN, Li Q, Fan JY, Pan CS, Liu YY, Yan L, Sun K, Hu BH, Huang DD, Zhao XR, Chang X, Wang CS, He SY, He K, Yang BX, Han JY Tags: Free Radic Biol Med Source Type: research

VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC.
In this study, VAOS exhibited effective inhibitory effects in NSCLC both in cultured cells and in a xenograft mouse model. VAOS was further identified to induce NSCLC cell apoptosis through activating protein kinase B (AKT) to elevate intracellular reactive oxygen species (ROS) levels by increasing in oxygen consumption and impairing the ROS-scavenging system. Neither silencing of PTP1B by siRNA nor transient overexpression of PTP1B had an effect on the AKT phosphorylation triggered by VAOS, indicating that PTP1B inhibition was not involved in VAOS-induced apoptosis. Through phosphorus colorimetric assay, we demonstrated t...
Source: Free Radical Biology and Medicine - September 14, 2018 Category: Biology Authors: Zhou L, Yi Y, Yuan Q, Zhang J, Li Y, Wang P, Xu M, Xie S Tags: Free Radic Biol Med Source Type: research

Cisplatin resistance involves a metabolic reprogramming through ROS and PGC-1 α in NSCLC which can be overcome by OXPHOS inhibition.
CONCLUSION: These results describe a new cisplatin resistance mechanism in NSCLC based on a metabolic reprogramming that is therapeutically exploitable through PGC-1α downregulation or OXPHOS inhibitors. PMID: 30880247 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - March 13, 2019 Category: Biology Authors: Cruz-Bermúdez A, Laza-Briviesca R, Vicente-Blanco RJ, García-Grande A, Coronado MJ, Laine-Menéndez S, Palacios-Zambrano S, Moreno-Villa MR, Ruiz-Valdepeñas AM, Lendinez C, Romero A, Franco F, Calvo V, Alfaro C, Acosta PM, Salas C, Garcia JM, Provencio Tags: Free Radic Biol Med Source Type: research

Chlorogenic acid promotes the Nrf2/HO-1 anti-oxidative pathway by activating p21Waf1/Cip1 to resist dexamethasone-induced apoptosis in osteoblastic cells.
Abstract In a previous study, p21Waf1/Cip1 (p21) promoted activation of the nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway, which has an important role in regulating apoptosis triggered by oxidative stress and inhibiting development of osteoporosis. Chlorogenic acid (CGA) has a strong protective effect on osteoporosis, closely related to activating the Nrf2/HO-1 pathway. However, whether CGA can resist apoptosis by regulating p21 and consequently promote activation of the Nrf2/HO-1 pathway needs further investigation. MC3T3-E1 cells were treated with dexamethasone (Dex), with or without ...
Source: Free Radical Biology and Medicine - April 16, 2019 Category: Biology Authors: Han D, Gu X, Gao J, Wang Z, Liu G, Barkema HW, Han B Tags: Free Radic Biol Med Source Type: research

TLR4 activation alters labile heme levels to regulate BACH1 and heme oxygenase-1 expression in macrophages.
In conclusion, alterations of labile heme levels in macrophages following TLR4 stimulation play a crucial role in BACH1-mediated regulation of HO-1 expression. PMID: 31026585 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - April 22, 2019 Category: Biology Authors: Sudan K, Vijayan V, Madyaningrana K, Gueler F, Igarashi K, Foresti R, Motterlini R, Immenschuh S Tags: Free Radic Biol Med Source Type: research

CHK1-CENP B/MAD2 is associated with mild oxidative damage-induced sex chromosome aneuploidy of male mouse embryos during in vitro fertilization.
Abstract A high incidence of aneuploidy is observed in in vitro fertilization (IVF)-derived embryos, but the formation and repair mechanisms are unknown. Here, we investigated the effects of slightly increased reactive oxygen species (ROS) produced by in vitro culture conditions on embryo aneuploidy and the roles of the spindle assembly checkpoint (SAC) protein, mitotic arrest-deficient 2 (MAD2), and the DNA damage response (DDR) protein, checkpoint kinase 1 (CHK1), in aneuploidy repair. By assessing chromosome abnormalities via DAPI staining, karyotype analysis and next-generation sequencing technology, we demons...
Source: Free Radical Biology and Medicine - April 27, 2019 Category: Biology Authors: Huang Y, Ha S, Li Z, Li J, Xiao W Tags: Free Radic Biol Med Source Type: research

S-1-propenylmercaptocysteine protects murine hepatocytes against oxidative stress via persulfidation of Keap1 and activation of Nrf2.
This study provides insights on the potential of CySSPe as an onion-derived dietary agent that modulates redox homeostasis and combats oxidative stress. PMID: 31349040 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - July 22, 2019 Category: Biology Authors: Tocmo R, Parkin K Tags: Free Radic Biol Med Source Type: research

Mitochondrial oxidative stress-induced transcript variants of ATF3 mediate lipotoxic brain microvascular injury.
Conclusions: TL potentiate ROS by mitochondria which activate mitochondrial oxidative stress, decrease ATP production, increase mitochondrial proton leak and glycolysis rate, and mitochondria DNA damage. Additionally, CPT1A1 siRNA knockdown suppresses oxidative stress and prevents mitochondrial dysfunction and vascular inflammation in TL treated HBMECs. TL activates ATF3-MAPKinase, TNF, and NRF2 signaling pathways. Furthermore, the NRF2 signaling pathway which is upstream of the ATF3-MAPKinase signaling pathway, is also regulated by the mitochondrial oxidative stress. We are the first to report differential inflammatory ch...
Source: Free Radical Biology and Medicine - July 25, 2019 Category: Biology Authors: Nyunt T, Britton M, Wanichthanarak K, Budamagunta M, Voss JC, Wilson DW, Rutledge JC, Aung HH Tags: Free Radic Biol Med Source Type: research

Free heme regulates placenta growth factor through NRF2-antioxidant response signaling.
Abstract Free heme activates erythroblasts to express and secrete Placenta Growth Factor (PlGF), an angiogenic peptide of the VEGF family. High circulating levels of PlGF have been associated in experimental animals and in patients with sickle cell disease with echocardiographic markers of pulmonary hypertension, a life-limiting complication associated with more intense hemolysis. We now show that the mechanism of heme regulation of PlGF requires the contribution of the key antioxidant response regulator NRF2. Mimicking the effect of heme, the NRF2 agonist sulforaphane stimulates the PlGF transcript level nearly 3...
Source: Free Radical Biology and Medicine - August 9, 2019 Category: Biology Authors: Kapetanaki MG, Gbotosho OT, Sharma D, Weidert F, Ofori-Acquah SF, Kato GJ Tags: Free Radic Biol Med Source Type: research

Oxidized base 8-oxoguanine, a product of DNA repair processes, contributes to dendritic cell activation.
Abstract A growing body of evidence suggests that elevated levels of reactive oxygen species (ROS) in the airways caused by exposure to gas phase pollutants or particulate matter are able to activate dendritic cells (DCs); however, the exact mechanisms are still unclear. When present in excess, ROS can modify macromolecules including DNA. One of the most abundant DNA base lesions is 7,8-dihydro-8-oxoguanine (8-oxoG), which is repaired by the 8-oxoguanine DNA glycosylase 1 (OGG1)-initiated base excision repair (BER) pathway. Studies have also demonstrated that in addition to its role in repairing oxidized purines, ...
Source: Free Radical Biology and Medicine - August 9, 2019 Category: Biology Authors: Pázmándi K, Sütő M, Fekete T, Varga A, Boldizsár E, Boldogh I, Bácsi A Tags: Free Radic Biol Med Source Type: research

Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.
This study investigated the role of cristae remodelling in palmitate (PA)-induced neonatal rat cardiomyocyte hypertrophy and explored the beneficial effects of ACh. We found loose, fragmented and even lysed cristae in PA-treated neonatal cardiomyocytes along with declines in mitochondrial network and complex expression and overproduction of mitochondrial reactive oxygen species (ROS); these changes ultimately resulted in increased myocardial size. Overexpression of mitofilin by adenoviral infection partly improved cristae shape, mitochondrial network, and ATP content and attenuated cell hypertrophy. Interestingly, siRNA-me...
Source: Free Radical Biology and Medicine - September 21, 2019 Category: Biology Authors: Xue RQ, Zhao M, Wu Q, Yang S, Cui YL, Yu XJ, Liu JK, Zang WJ Tags: Free Radic Biol Med Source Type: research

The uricosuric benzbromarone disturbs the mitochondrial redox homeostasis and activates the NRF2 signaling pathway in HepG2 cells.
In conclusion, benzbromarone increases mitochondrial O2•- accumulation and activates the NRF2 signaling pathway in HepG2 cells, thereby strengthening the cytosolic and mitochondrial antioxidative defense. Impaired antioxidative defense may represent a risk factor for benzbromarone-induced hepatotoxicity. PMID: 32198009 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - March 16, 2020 Category: Biology Authors: Roos NJ, Duthaler U, Bouitbir J, Krähenbühl S Tags: Free Radic Biol Med Source Type: research

Down regulation of glutathione and glutamate cysteine ligase in the inflammatory response of macrophages.
In conclusion, these data suggest that GCLC down-regulation in the inflammatory response of macrophages is mediated through both increased mRNA decay and caspase-5-mediated GCLC protein degradation, and γ-GC is an efficient agent to restore GSH and regulate the inflammatory response. PMID: 32682927 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - July 15, 2020 Category: Biology Authors: Zhang H, Zhang SJ, Lyn N, Florentino A, Li A, Davies KJA, Forman HJ Tags: Free Radic Biol Med Source Type: research

Angelica dahurica promoted angiogenesis and accelerated wound healing in db/db mice via the HIF-1 α/PDGF-β signaling pathway.
In this study, we found that Angelica dahurica (A. dahurica) stimulated angiogenesis and benefited wound healing in genetic mouse models of diabetes. In HUVECs, A. dahurica promoted cell proliferation and tube formation, which was accompanied by increased nuclear translocation of HIF-1α under hypoxic conditions and led to elevated PDGF-β protein expression. A. dahurica activated the PI3K/AKT signaling pathway in human umbilical vein endothelial cells (HUVECs), which was abrogated by the PI3K inhibitor LY294002. Furthermore, the cellular expression of PDGF-β decreased significantly after treatment with a HIF-1α-siRNA, a...
Source: Free Radical Biology and Medicine - August 23, 2020 Category: Biology Authors: Guo J, Hu Z, Yan F, Lei S, Li T, Li X, Xu C, Sun B, Pan C, Chen L Tags: Free Radic Biol Med Source Type: research