Caffeic Acid Attenuates Rat Liver Injury after Transplantation Involving PDIA3-Dependent Regulation of NADPH Oxidase.

This study was undertaken to explore the effect of Caffeic acid (CA), a naturally occurring antioxidant, on I/R injury of grafted liver and the mechanisms involved. Male Sprague-Dawley rats underwent orthotopic liver transplantation (LT) in the absence or presence of CA administration. In vitro, HL7702 cells were subjected to hypoxia/reoxygenation. LT led to apparent hepatic I/R injury, manifested by deteriorated liver function, microcirculatory disturbance and increased apoptosis, along with increased PDIA3 expression and nicotinamide adenosine dinucleotide phosphate (NADPH) oxidase activity, and membrane translocation of NADPH oxidase subunits. Treatment with CA attenuated the above alterations. siRNA-mediated knockdown of PDIA3 in HL7702 cells and rats played the same role as CA not only in inhibiting ROS production and NADPH oxidase activity, but also in alleviating hepatocytes injury. CA protects transplanted livers from injury, which is likely attributed to its protection of oxidative damage by interfering in PDIA3-dependent activation of NADPH oxidase.
PMID: 30218773 [PubMed - as supplied by publisher]

Source: Free Radical Biology and Medicine - September 12, 2018 Category: Biology Authors: Mu HN, Li Q, Fan JY, Pan CS, Liu YY, Yan L, Sun K, Hu BH, Huang DD, Zhao XR, Chang X, Wang CS, He SY, He K, Yang BX, Han JY Tags: Free Radic Biol Med Source Type: research