F2-isoprostanes affect macrophage migration and csf-1 signalling.

F2-ISOPROSTANES AFFECT MACROPHAGE MIGRATION AND CSF-1 SIGNALLING.
Free Radic Biol Med. 2018 Aug 07;:
Authors: Proudfoot JM, Murrey MW, McLean S, Greenland EL, Barden AE, Croft KD, Galano JM, Durand T, Mori TA, Pixley FJ
Abstract
F2-isoprostanes (F2-IsoP) are formed in vivo via free radical peroxidation of arachidonic acid. Enhanced oxidative stress is implicated in the development of atherosclerosis in humans and F2-IsoP have been detected in atherosclerotic plaque. Colony stimulating factor-1 (CSF-1) is essential to macrophage survival, proliferation and differentiation and has been detected in human atherosclerotic plaques. Accumulation of macrophages within the vascular wall is an important component of atherosclerosis but little is known about the effect of F2-IsoP on the migration of these cells. Our aim was to examine the effect of free and lipid-bound 15-F2t-isoprostane (15-F2t-IsoP) on macrophage migration and investigate the signalling pathways involved. Mouse macrophages (cell line BAC1.2F5) were pre-incubated with 15-F2t-IsoP (free, bound to cholesterol or monoacylglycerol or within oxidized phospholipid) and cell migration was assessed using chemotaxis towards CSF-1 in Boyden chambers. Migration was also measured using the wound healing assay with human monocytes (cell line THP-1) differentiated into macrophages and primary mouse bone marrow derived macrophages. We showed that 15-F2t-IsoP dose-dependently inhibited BAC1.2...

Source: Free Radical Biology and Medicine - August 7, 2018 Category: Biology Authors: Proudfoot JM, Murrey MW, McLean S, Greenland EL, Barden AE, Croft KD, Galano JM, Durand T, Mori TA, Pixley FJ Tags: Free Radic Biol Med Source Type: research