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Source: Cell Biology International

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Total 159 results found since Jan 2013.

Cilostazol inhibits interleukin-1-induced ADAM17 expression through cAMP independent signaling in vascular smooth muscle cells.
Abstract Increased a disintegrin and metalloprotease 17 (ADAM17) expression in vascular smooth muscle cells (VSMC) is implicated in the development of cardiovascular diseases including atherosclerosis and hypertension. Although cilostazol, type phosphodiesterase (PDE?) inhibitor, has recently been found to inhibit VSMC proliferation, the mechanisms remain largely unclear. Here we hypothesized that cilostazol regulates the ADAM17 expression in VSMC. In cultured VSMC, interleukin (IL)-1α and IL-1β significantly increased ADAM17 expression. MEK inhibitor U0126, NF-κB inhibitor BAY-11-7085 and siRNA targeting p65/R...
Source: Cell Biology International - October 30, 2015 Category: Cytology Authors: Takaguri A, Morimoto M, Imai SI, Satoh K Tags: Cell Biol Int Source Type: research

Wnt5A exerts immunomodulatory activity in the human ovarian cancer cell line SKOV-3.
This study revealed a previously unrecognized immunomodulatory role of endogenous Wnt5A in ovarian cancer cells, which could further influence chemotactic cell migration. PMID: 26462870 [PubMed - as supplied by publisher]
Source: Cell Biology International - October 13, 2015 Category: Cytology Authors: Arabzadeh S, Hossein G, Zarnani AH Tags: Cell Biol Int Source Type: research

IRE1α and TRB3 do not contribute to the disruption of proximal insulin signaling caused by palmitate in C2C12 myotubes.
Abstract Endoplasmic reticulum (ER) stress is a central actor in the physiopathology of insulin resistance (IR) in various tissues. The subsequent unfolded protein response (UPR) interacts with insulin signaling through inositol-requiring 1α (IRE1α) activation and tribbles homolog 3 (TRB3) expressions. IRE1α impairs insulin actions through the activation of c-Jun N-terminal kinase (JNK) and TRB3 is a pseudokinase inhibiting Akt. In muscle cells, the link between ER stress and IR has only been demonstrated by using chemical ER stress inducers or overexpression techniques. However, the involvement of ER stress in...
Source: Cell Biology International - September 4, 2015 Category: Cytology Authors: Pierre N, Fernández-Verdejo R, Regnier P, Vanmechelen S, Demeulder B, Francaux M Tags: Cell Biol Int Source Type: research

Gab1 amplifies signalling in response to low-intensity stimulation by HGF.
Abstract The receptor tyrosine kinases EGFR and Met induce phosphorylation of the docking protein Gab1, and there is evidence that Gab1 may have a role in the signalling from these receptors. Studying hepatocytes, we previously found that although Gab1 mechanistically interacted in different ways with EGFR and Met, it was involved in mitogenic signalling induced by both EGF and HGF. It has been reported that in EGFR signalling Gab1 is required particularly at a low dose of EGF. Whether this also applies to HGF/Met signalling has not been investigated. We have studied the role of Gab1 in activation of the Akt and E...
Source: Cell Biology International - July 4, 2015 Category: Cytology Authors: Aasrum M, Ødegård J, Thoresen GH, Brusevold IJ, Sandnes DL, Christoffersen T Tags: Cell Biol Int Source Type: research

Expression and Function of Fatty Acid-binding Protein 4 in Epithelial Cell of Uterine Endometrium.
Abstract The aims of this study were to delineate the expression of fatty-acid binding protein (FABP) 4 in human uterine endometrium and its function in the regulation of proliferation, migration and invasion of epithelial cells. Immunohistochenistry, immunofluorence and Western blotting were used to determine the expression and cellular localization of FABP4 in endometrium and endometrial epithelial cell lines. Interference of small ribonuclear acid (siRNA) and specific FABP4 inhibitor were used to inhibit FABP4. The proliferation, migration and invasion of epithelial cells were evaluated with CCK-8 assay, wound-...
Source: Cell Biology International - January 9, 2015 Category: Cytology Authors: Zhu Q, Jin Y, Wang P, Wang H, Lu B, Wang Z, Dong M Tags: Cell Biol Int Source Type: research

Knockdown of tumor protein D52-like 2 induces cell growth inhibition and apoptosis in oral squamous cell carcinoma.
Abstract Tumor protein D52-like 2 (TPD52L2) and its family members form homo- and heteromeric complexes essential for cell proliferation in multiple human cancers. TPD52L2 is involved in cell migration and attachment in oral squamous cell carcinoma (OSCC). To confirm the role of TPD52L2 in OSCC, we employed the lentivirusdelivered small interfering RNA (siRNA) technique to knock down TPD52L2 expression in 2 OSCC cell lines, CAL27 and KB. Knockdown of TPD52L2 by RNA interference markedly suppressed cell proliferation and colony formation. Cell cycle analysis showed that depletion of TPD52L2 led to CAL27 cells arres...
Source: Cell Biology International - September 27, 2014 Category: Cytology Authors: He Y, Chen F, Cai Y, Chen S Tags: Cell Biol Int Source Type: research

MKP-1 is a target of miR-210 and mediate the negative regulation of miR-210 inhibitor on hypoxic hPASMC proliferation.
Abstract Chonic hypoxia, smooth muscle cell (SMC) proliferation and vascular remodeling are hallmark features of pathogenic pulmonary artery hypertension. MicroRNAs (miRNAs), endogenously expressed small noncoding RNAs, regulate gene expression at the post-transcriptional level. MiR-210 is considered a "master miRNA" in the control of diverse functions in hypoxic cells and tissues and has a cytoprotective function in pulmonary artery SMCs during hypoxic stress. MiR-210 is also upregulated in lung tissue of chonically hypoxic mice suffering from pulmonary hypertension. Yi et al. (XXXX) showed that mice deficient in...
Source: Cell Biology International - July 15, 2014 Category: Cytology Authors: Jin Y, Pang T, Nelin LD, Wang W, Wang Y, Yan J, Zhao C Tags: Cell Biol Int Source Type: research

Bcl-2 down-regulation by small interfering RNA induces Beclin1-dependent autophagy in human SGC-7901 cells.
Abstract While Bcl-2 protein is involved in the regulation of apoptosis, recent research showed that Beclin1, described as the essential autophagy effector and haploinsufficent tumour suppressor, was originally isolated as a Bcl-2 interacting protein. Beclin1 interacts with Bcl-2 through a BH3 domain; nevertheless, the function of the anti-apoptotic gene, Bcl-2, in autophagy is not well understood. We explored the role of Bcl-2 in autophagy in human SGC-7901 cells in which Bcl-2 is overexpressed. Knockdown of Bcl-2 by small interfering RNA in human SGC-7901 cells downregulated Bcl-2 protein levels ∼82% and induc...
Source: Cell Biology International - July 7, 2014 Category: Cytology Authors: Du Y, Ji X Tags: Cell Biol Int Source Type: research

Gene expression profile analysis of SUDHL6 cells with siRNA-mediated BCL11 A downregulation.
Abstract Our previous study has shown that downregulation of B-cell chronic lymphocytic leukemia (CLL)/lymphoma11A (BCL11A) gene by small interfering RNA (siRNA) results in the growth inhibition and apoptosis of B cell lymphoma cell line SUDHL6. To gain further insight into the molecular mechanisms of this process and identify the differentially expressed genes in SUDHL6 cells after BCL11A downregulation, the global gene expression profile was identified and analyzed using the Affymetrix HG-U133 Plus 2.0 array.Twenty-one differentially expressed genes were validated and analyzed from the BCL11 A siRNA-treated SUDH...
Source: Cell Biology International - July 7, 2014 Category: Cytology Authors: Wu H, Li Y Tags: Cell Biol Int Source Type: research

IL-8 enhances angiogenesis of human bone marrow mesenchymal stem cells by increasing vascular endothelial growth factor.
In conclusion, IL-8 stimulates VEGF production in hBM-MSCs in part via the PI3K/Akt and MAPK/ERK signal transduction pathways and that administration of IL-8-treated hBM-MSCs increases angiogenesis after stroke. This approach may be used to optimize MSC-based therapies for numerous diseases including stroke, myocardial ischemia, and spinal cord injury. PMID: 24797366 [PubMed - as supplied by publisher]
Source: Cell Biology International - May 5, 2014 Category: Cytology Authors: Hou Y, Ryu CH, Jun JA, Kim SM, Jeong CH, Jeun SS Tags: Cell Biol Int Source Type: research

Regulation of chondrocyte proliferation through GIT1-Rac1-mediated ERK1/2 pathway by PDGF.
Abstract There are many growth factors contributing to fracture healing after bone fractures. Platelet-derived growth factor (PDGF) released from platelets is a factor promoting cell division and proliferation, and first appears around the sites of fractures. Culture of chondrocytes in vitro are stimulated by PDGF to proliferation, its presence being upregulated in the extracellular matrix of cartilage; the main components include aggrecan and type Ⅱ collagen. PDGF induces the expression of G the protein-coupled receptor kinase interacting protein 1 (GIT1), promoting Rac1 and ERK1/2 phosphorylation. Both knockin...
Source: Cell Biology International - January 13, 2014 Category: Cytology Authors: Xiao J, Chen X, Xu L, Zhang Y, Yin Q, Wang F Tags: Cell Biol Int Source Type: research

Fibronectin promotes migration and invasion of ovarian cancer cells through up-regulation of FAK-PI3K/Akt pathway.
This study investigates the mechanism of fibronectin (Fn) in stimulating ovarian cancer cell migration and invasion through up-regulation of FAK pathway. Human ovarian cancer cells (OVCAR-3, A2780/CP70) were cultured and treated with fibronectin (10 µg/ml). Trans-well plates were used to conduct the migration assay, real-time RT-PCR for FAK mRNA expression, and FAK siRNA for blocking FAK expression. Western blots were used for P-FAK, P-PI3K and P-Akt analysis. Fibronectin treated OVCAR-3, A2780/CP70 cells have increased ability to migrate and invade. It significantly promoted this behaviour through the phosphorylation o...
Source: Cell Biology International - September 21, 2013 Category: Cytology Authors: Yousif NG Tags: Cell Biol Int Source Type: research

Inhibition of mTOR signaling potentiates the effects of trichostatin A in human gastric cancer cell lines by promoting histone acetylation.
Abstract Deregulation of the mammalian target of rapamycin pathway (mTOR pathway) is associated with human cancer. The relationship between mTOR pathway and histone acetylation is still unclear in gastric cancer. Immunohistochemistry was used to examine the phosphorylation of mTOR and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) in gastric cancer (GC) tissues. MKN45 and SGC7901 cells were treated with the mTOR inhibitor rapamycin (RAPA) alone or in combination with the phosphatidylinositol 3-kinase inhibitor LY294002 and the histone deacetylase (HDAC) inhibitor trichostatin A (TSA). Small...
Source: Cell Biology International - September 13, 2013 Category: Cytology Authors: Sun DF, Zhang YJ, Tian XQ, Chen YX, Fang JY Tags: Cell Biol Int Source Type: research

Calpains are required for invasive and metastatic potentials of human HCC cells.
Abstract Calpains are a conserved family of calcium-dependent cysteine proteinases involved in various cellular functions. Two ubiquitous isoforms, µ- and m-calpain, are key members of the calpain family that play essential roles in regulating cell migration and invasion. However, it remains unclear whether they are involved in the progression of hepatocellular carcinoma (HCC). Here, we investigated the functions of µ- and m-calpain in the invasive and metastatic processes of human hepatoma cells. Our results indicated that the expression levels of calpains were elevated in HCC cells compared with those in norma...
Source: Cell Biology International - June 7, 2013 Category: Cytology Authors: Chen B, Tang J, Guo YS, Li Y, Chen ZN, Jiang JL Tags: Cell Biol Int Source Type: research

Aurora B kinase is required for cytokinesis through effecting spindle structure.
Abstract The spindle, composed of microtubules and associated proteins, serves as major machinery for cell division. Aurora B is a chromosome passenger protein that has important functions in mitosis. Its dynamic distribution at mitosis goes along with spindle structure and dynamics. We used the siRNA technique to knockdown protein expression and immunofluorescence technique to follow Aurora B during mitosis. Aurora B regulates microtubule plus ends as mitosis progresses, including both kinetochore and polar microtubules. Interactions between Aurora B and polar microtubule plus ends lead to failure in cytokinesis ...
Source: Cell Biology International - May 1, 2013 Category: Cytology Authors: Wu Q, Zhang W, Mu T, Song T, Li D Tags: Cell Biol Int Source Type: research