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Source: Journal of Cellular Physiology

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Total 116 results found since Jan 2013.

Regulation of cellular processes by interleukin‐16 in homeostasis and cancer
This article will review the cellular process for generating IL‐16, the biological activities for both the pro‐ and secreted forms of the protein, and then the mechanism by which these forms contribute to cancer progression. As a soluble cytokine the ability to reduce or eliminate IL‐16 synthesis through siRNA approaches or bioactivity through the use of neutralizing antibody treatment may represent a novel therapeutic approach. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.
Source: Journal of Cellular Physiology - July 29, 2013 Category: Cytology Authors: Jillian Richmond, Marina Tuzova, William Cruikshank, David Center Tags: Mini‐Review Source Type: research

TRPM7 channels regulate proliferation and adipogenesis in 3T3‐L1 preadipocytes
Abstract Transient receptor potential melastatin‐7 (TRPM7) channels are involved in many cellular physiological and pathological processes. The present study was designed to investigate the expression of TRPM7 channels and the potential role in regulating cell proliferation and adipogenesis in 3T3‐L1 preadipocytes with approaches of whole‐cell patch voltage‐clamp, molecular biology, cell proliferation, adipogenesis, etc. We found that a TRPM7‐like current was recorded with Mg2+‐free pipette solution in 3T3‐L1 preadipocytes, and the current was inhibited by intercellular free Mg2+. The TRPM7‐like current was...
Source: Journal of Cellular Physiology - June 14, 2013 Category: Cytology Authors: Kui‐Hao Chen, Xiao‐Hui Xu, Yi Liu, Yan Hu, Man‐Wen Jin, Gui‐Rong Li Tags: Original Research Article Source Type: research

Testosterone increases GLUT4‐dependent glucose uptake in cardiomyocytes
Abstract Testosterone exerts important effects in the heart. Cardiomyocytes are target cells for androgens, and testosterone induces rapid effects via Ca2+ release and protein kinase activation and long‐term effects via cardiomyocyte differentiation and hypertrophy. Furthermore, it stimulates metabolic effects such as increasing glucose uptake in different tissues. Cardiomyocytes preferentially consume fatty acids for ATP production, but under particular circumstances, glucose uptake is increased to optimize energy production. We studied the effects of testosterone on glucose uptake in cardiomyocytes. We found that testo...
Source: Journal of Cellular Physiology - June 11, 2013 Category: Cytology Authors: Carlos Wilson, Ariel Contreras‐Ferrat, Nathaly Venegas, César Osorio‐Fuentealba, Mario Pávez, Katherine Montoya, Javier Durán, Rodrigo Maass, Sergio Lavandero, Manuel Estrada Tags: Original Research Article Source Type: research

Snail mediates PDGF‐BB‐induced invasion of rat bone marrow mesenchymal stem cells in 3D collagen and chick chorioallantoic membrane
Abstract We previously reported that membrane type‐1 matrix metalloproteinase (MT1‐MMP) enables mesenchymal stem cells (MSCs) to move through both three‐dimensional (3D) type I collagen and basement membrane barriers; however, its upstream regulating factors were unidentified. Here, we report that PDGF‐BB upregulates both mRNA and protein expression of snail in rat bone marrow MSCs (rBMMSCs). PDGF‐BB enhances rBMMSC invasion in 3D collagen, which is blocked by snail specific siRNA transfection. Snail overexpression induced by plasmid transfection results in increased rBMMSC invasion in 3D collagen. Snail expressi...
Source: Journal of Cellular Physiology - May 25, 2013 Category: Cytology Authors: Changlian Lu, Xiaojiao Sun, Lijun Sun, Jianping Sun, Yan Lu, Xin Yu, Lingyun Zhou, Xu Gao Tags: Original Research Article Source Type: research

Akt inhibition promotes hexokinase 2 redistribution and glucose uptake in cancer cells
Abstract Hexokinase II (HK2), the enzyme that catalyzes the first committed step of glycolysis, is overexpressed in many cancers, as is the central signaling kinase Akt. Akt activity promotes HK2 association with the mitochondria, as well as glucose uptake by cancer cells. In HeLa cervical cancer cells, Akt inhibitor IV (Ai4) increased nuclear HK2 localization, while in MDA‐MB‐231 breast cancer cells, Ai4 merely induced cytoplasmic redistribution without increased nuclear accumulation. Small interfering RNA (siRNA) directed against Akt confirmed the effect in HeLa cells. Next, we treated the cells with clotrimazole (CT...
Source: Journal of Cellular Physiology - April 30, 2013 Category: Cytology Authors: Catherine L. Neary, John G. Pastorino Tags: Original Research Article Source Type: research

Activation and promotion of adipose stem cells by tumour necrosis factor‐alpha preconditioning for bone regeneration
This study investigated the effect of short‐term (3 days) preconditioning with tumor necrosis factor‐alpha (TNF‐α) on proliferation, mobilization, and differentiation of adipose tissue‐derived mesenchymal stem cells (ASCs). We demonstrated that TNF‐α pre‐conditioning increased proliferation, mobilization, and osteogenic differentiation of ASCs and up‐regulated bone morphogenetic protein‐2 (BMP‐2) protein level. BMP‐2 silencing by siRNA partially inhibited osteogenic differentiation of ASCs induced by TNF‐α; BMP‐2 pre‐conditioning also significantly increased osteogenic differentiation of ASCs b...
Source: Journal of Cellular Physiology - April 18, 2013 Category: Cytology Authors: ZuFu Lu, GuoCheng Wang, Colin R. Dunstan, YongJun Chen, William Yenn‐Ru Lu, Ben Davies, Hala Zreiqat Tags: Original Research Article Source Type: research

MicroRNA‐195 chemosensitizes colon cancer cells to the chemotherapeutic drug doxorubicin by targeting the first binding site of BCL2L2 mRNA
In this study, we utilized microRNA array and real‐time PCR to verify that some microRNAs including miR‐127, miR‐195, miR‐22, miR‐137 were significantly down‐regulated, while miR‐21, miR‐592 were up‐regulated in both HT29/DOX and LOVO/DOX cell lines. In vitro cell viability assay showed that knockdown of miR‐195 in HT29 and LOVO cells caused a marked inhibition of Dox‐induced cytotoxicity. Moreover, we explored that miR‐195 is involved in repression of BCL2L2 expression through targeting its 3'‐untranslated region, especially the first binding site within its mRNA. Furthermore, down‐regulation o...
Source: Journal of Cellular Physiology - March 22, 2013 Category: Cytology Authors: Juan Qu, Liang Zhao, Pengzhi Zhang, Juan Wang, Ning Xu, Wenjuan Mi, Xingwang Jiang, Changming Zhang, Juan Qu Tags: Original Research Article Source Type: research

CCL20 induces migration and proliferation on breast epithelial cells
Abstract The communication between the tumour cells and the surrounding cells helps drive the process of tumour progression. Since the microenvironment of breast cancer includes CCL20 chemokine, the purpose of this study was to determine whether CCL20 modulates the physiology of healthy breast epithelial cells in areas adjacent to the tumour. Therefore, primary cultures of mammary cells taken from normal peritumoral areas were used. We assessed that breast cells expressed CCR6 CCL20 receptor. Using molecular (siRNA) and pharmacological (inhibitors) techniques we found multiple signalling kinases to be activated by CCR6 and...
Source: Journal of Cellular Physiology - March 4, 2013 Category: Cytology Authors: Santo Marsigliante, Carla Vetrugno, Antonella Muscella Tags: Original Research Article Source Type: research

Snail mediates PDGF‐BB‐induced invasion of rat bone marrow mesenchymal stem cells in 3‐D collagen and chick chorioallantoic membrane
Abstract We previously reported that membrane type‐1 matrix metalloproteinase (MT1‐MMP) enables mesenchymal stem cells (MSCs) to move through both three‐dimensional (3D) type I collagen and basement membrane barriers; however, its upstream regulating factors were unidentified. Here, we report that PDGF‐BB upregulates both mRNA and protein expression of snail in rat bone marrow MSCs (rBMMSCs). PDGF‐BB enhances rBMMSC invasion in 3D collagen, which is blocked by snail specific siRNA transfection. Snail overexpression induced by plasmid transfection results in increased rBMMSC invasion in 3D collagen. Snail expressi...
Source: Journal of Cellular Physiology - March 4, 2013 Category: Cytology Authors: Changlian Lu, Xiaojiao Sun, Lijun Sun, Jianping Sun, Yan Lu, Xin Yu, Lingyun Zhou, Xu Gao Tags: Original Research Article Source Type: research

Omega‐3 PUFA ethanolamides DHEA and EPEA induce autophagy through PPARγ activation in MCF‐7 breast cancer cells
Abstract The omega‐3 long chain polyunsaturated fatty acids, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), elicit anti‐proliferative effects in cancer cell lines and in animal models. Dietary DHA and EPA can be converted to their ethanolamide derivatives, docosahexaenoyl ethanolamine (DHEA), and eicosapentaenoyl ethanolamine (EPEA), respectively; however, few studies are reported on their anti‐cancer activities. Here, we demonstrated that DHEA and EPEA were able to reduce cell viability in MCF‐7 breast cancer cells whereas they did not elicit any effects in MCF‐10A non‐tumorigenic breast epitheli...
Source: Journal of Cellular Physiology - February 25, 2013 Category: Cytology Authors: Daniela Rovito, Cinzia Giordano, Donatella Vizza, Pierluigi Plastina, Ines Barone, Ivan Casaburi, Marilena Lanzino, Francesca De Amicis, Diego Sisci, Loredana Mauro, Saveria Aquila, Stefania Catalano, Daniela Bonofiglio, Sebastiano Andò Tags: Original Research Article Source Type: research

Activation and promotion of adipose tissue‐derived mesenchymal stem cells by tumour necrosis factor‐alpha preconditioning for bone tissue engineering
This study investigated the effect of short‐term (three days) preconditioning with tumour necrosis factor‐alpha (TNF‐α) on proliferation, mobilization and differentiation of adipose tissue‐derived mesenchymal stem cells (ASCs). We demonstrated that TNF‐α pre‐conditioning increased proliferation, mobilization, and osteogenic differentiation of ASCs and up‐regulated bone morphogenetic protein‐2 (BMP‐2) protein levels. BMP‐2 silencing by siRNA partially inhibited osteogenic differentiation of ASCs induced by TNF‐α; BMP‐2 pre‐conditioning also significantly increased osteogenic differentiation of A...
Source: Journal of Cellular Physiology - January 28, 2013 Category: Cytology Authors: ZuFu Lu, GuoCheng Wang, Colin R Dunstan, YongJun Chen, William Yenn‐Ru Lu, Ben Davies, Hala Zreiqat Tags: Original Research Article Source Type: research