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Source: Journal of Cellular Physiology
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Total 116 results found since Jan 2013.
Effects of small interfering RNA targeting TLR4 on expressions of adipocytokines in obstructive sleep apnea hyponea syndrome with hypertension in a rat model
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Physiology - December 7, 2017 Category: Cytology Authors: Jing ‐Guo Wu, Nan Xun, Li‐Jin Zeng, Zhen‐Yu Li, Yan‐Bing Liang, Hao Tang, Zhong‐Fu Ma Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Glucosamine‐Induced Sp1 O‐GlcNAcylation Ameliorates Hypoxia‐Induced SGLT Dysfunction in Primary Cultured Renal Proximal Tubule Cells
The aim of this study is to determine whether GlcN could recover the endoplasmic reticulum (ER) stress‐induced dysfunction of Na+/glucose cotransporter (SGLT) in renal proximal tubule cells (PTCs) under hypoxia. With the rabbit model, the renal ischemia induced tubulointerstitial abnormalities and decreased SGLTs expression in tubular brush‐border, which were recovered by GlcN. Thus, the protective mechanism of GlcN against renal ischemia was being examined by using PTCs. Hypoxia decreased the level of protein O‐GlcNAc and the expression of O‐GlcNAc transferase (OGT) while increased O‐GlcNAcase (OGA) and these we...
Source: Journal of Cellular Physiology - March 4, 2014 Category: Cytology Authors: Han Na Suh, Yu Jin Lee, Mi Ok Kim, Ho Jae Han Tags: Original Research Article Source Type: research
Interleukin‐17A increases cell migration and MMP‐1 expression in human periodontal ligament fibroblasts via p38 MAPK/NF‐κB ‐dependent pathway
In this study, we report that IL‐17A can increase the migration and expression of matrix metalloproteinase (MMP)‐1 in human periodontal ligament (PDL) fibroblasts but has no effect on PDL fibroblast proliferation. IL‐17A‐induced MMP‐1 expression led to cell migration, which was attenuated by pre‐treatment with IL‐17 receptor neutralizing antibody and small interfering RNA (siRNA) for MMP‐1. The IL‐17A‐induced cell migration was also attenuated by its tissue inhibitor of matrix metalloproteinase (TIMP)‐1. In addition, a p38 mitogen‐activated protein kinase (MAPK) inhibitor (SB203580) inhibited IL‐1...
Source: Journal of Cellular Physiology - August 8, 2013 Category: Cytology Authors: Yan Wu, Lingxin Zhu, Lingshuang Liu, Jie Zhang, Bin Peng Tags: Original Research Article Source Type: research
Interleukin‐17A Stimulates Migration of Periodontal Ligament Fibroblasts Via p38 MAPK/NF‐κB ‐Dependent MMP‐1 Expression
In this study, we report that IL‐17A can increase the migration and expression of matrix metalloproteinase (MMP)‐1 in human periodontal ligament (PDL) fibroblasts but has no effect on PDL fibroblast proliferation. IL‐17A‐induced MMP‐1 expression led to cell migration, which was attenuated by pre‐treatment with IL‐17 receptor neutralizing antibody and small interfering RNA (siRNA) for MMP‐1. The IL‐17A‐induced cell migration was also attenuated by its tissue inhibitor of matrix metalloproteinase (TIMP)‐1. In addition, a p38 mitogen‐activated protein kinase (MAPK) inhibitor (SB203580) inhibited IL‐1...
Source: Journal of Cellular Physiology - November 22, 2013 Category: Cytology Authors: Yan Wu, Lingxin Zhu, Lingshuang Liu, Jie Zhang, Bin Peng Tags: Original Research Article Source Type: research
Snail mediates PDGF‐BB‐induced invasion of rat bone marrow mesenchymal stem cells in 3‐D collagen and chick chorioallantoic membrane
Abstract
We previously reported that membrane type‐1 matrix metalloproteinase (MT1‐MMP) enables mesenchymal stem cells (MSCs) to move through both three‐dimensional (3D) type I collagen and basement membrane barriers; however, its upstream regulating factors were unidentified. Here, we report that PDGF‐BB upregulates both mRNA and protein expression of snail in rat bone marrow MSCs (rBMMSCs). PDGF‐BB enhances rBMMSC invasion in 3D collagen, which is blocked by snail specific siRNA transfection. Snail overexpression induced by plasmid transfection results in increased rBMMSC invasion in 3D collagen. Snail expressi...
Source: Journal of Cellular Physiology - March 4, 2013 Category: Cytology Authors: Changlian Lu, Xiaojiao Sun, Lijun Sun, Jianping Sun, Yan Lu, Xin Yu, Lingyun Zhou, Xu Gao Tags: Original Research Article Source Type: research
Snail mediates PDGF‐BB‐induced invasion of rat bone marrow mesenchymal stem cells in 3D collagen and chick chorioallantoic membrane
Abstract
We previously reported that membrane type‐1 matrix metalloproteinase (MT1‐MMP) enables mesenchymal stem cells (MSCs) to move through both three‐dimensional (3D) type I collagen and basement membrane barriers; however, its upstream regulating factors were unidentified. Here, we report that PDGF‐BB upregulates both mRNA and protein expression of snail in rat bone marrow MSCs (rBMMSCs). PDGF‐BB enhances rBMMSC invasion in 3D collagen, which is blocked by snail specific siRNA transfection. Snail overexpression induced by plasmid transfection results in increased rBMMSC invasion in 3D collagen. Snail expressi...
Source: Journal of Cellular Physiology - May 25, 2013 Category: Cytology Authors: Changlian Lu, Xiaojiao Sun, Lijun Sun, Jianping Sun, Yan Lu, Xin Yu, Lingyun Zhou, Xu Gao Tags: Original Research Article Source Type: research
P53 Dependent Mitochondrial Permeability Transition Pore Opening Is Required for Dexamethasone‐Induced Death of Osteoblasts
Prolonged or overdose glucocorticoids (GCs) usage is the common cause of osteoporosis. In the present study, we studied the cellular mechanism of dexamethasone (Dex)‐induce osteoblast cell death by focusing on the role of mitochondrial permeability transition pore (mPTP). In cultured osteoblastic MC3T3‐E1 cells, Dex‐induced mPTP opening, which was demonstrated by mitochondrial membrane potential (MPP) decrease, cyclophilin‐D (CyPD)–adenine nucleotide translocator 1 (ANT‐1) mitochondrial complexation and cytochrome C (cyto‐C) release. The mPTP inhibitor sanglifehrin A (SfA) dramatically inhibited Dex‐induced...
Source: Journal of Cellular Physiology - February 25, 2014 Category: Cytology Authors: Yun‐fang Zhen, Guo‐dong Wang, Lun‐qing Zhu, Shi‐ping Tan, Fu‐yong Zhang, Xiao‐zhong Zhou, Xiao‐dong Wang Tags: Original Research Article Source Type: research
Short‐Term Hypoxia Enhances the Migratory Capability of Dendritic Cell Through HIF‐1α and PI3K/Akt Pathway
In this study we observed an enhanced migratory capability of human monocyte‐derived DC, using in vitro migration assays performed under hypoxic conditions. Such enhancement was independent on either the chemoattractants involved or the maturation level of DC. However, HIF‐1α appeared to be crucial for the migration only of immature DC and not for mature DC under hypoxia, as indicated by HIF‐1α siRNA approaches. Furthermore, we observed that while Akt phosphorylation was enhanced in both immature and mature DC exposed to hypoxia, other signaling pathways, such as p38 and p42/p44 MAPK, were differently affected duri...
Source: Journal of Cellular Physiology - May 12, 2014 Category: Cytology Authors: Irene Filippi, Emilia Morena, Carlo Aldinucci, Fabio Carraro, Silvano Sozzani, Antonella Naldini Tags: Original Research Article Source Type: research
The regulation of TGFβ1 induced fibronectin EDA exon alternative splicing in human renal proximal tubule epithelial cells
The EDA+ splice variant of fibronectin (Fn) is an early and important component of the extracellular matrix in renal fibrosis. In this work, we investigate cellular mechanisms of EDA+ Fn production in human primary proximal tubule epithelial cells (PTECs).TGFβ1‐induced EDA+Fn production was assessed by immunocytochemistry, PCR and Western blotting. SRp40 knockdown was achieved by siRNA. The role of the PI3 kinase‐AKT signalling and splicing regulatory protein SRp40 in the production of EDA+ Fn was studied by using the chemical inhibitor LY294002 and siRNA targeted to SRp40 respectively. Interaction between PI3 kinase...
Source: Journal of Cellular Physiology - June 24, 2014 Category: Cytology Authors: Mysore Keshavmurthy Phanish, Felicia Heidebrecht, Mohammad E Nabi, Nileshkumar Shah, Ioana Niculescu‐Duvaz, Mark Edward Carl Dockrell Tags: Original Research Article Source Type: research
Interleukin‐17 induces CC chemokine receptor 6 expression and cell migration in colorectal cancer cells
This study aimed to investigate the effect of IL‐17 on the cell migration of CRC cells. Human CRC HCT‐116 cells were used to study the effect of IL‐17 on CCR6 expression and cell migration in CRC cells. IL‐17 treatment induced migration of HCT‐116 cells across the Boyden chamber membrane and increased the expression level of the CCR6. Inhibition of CCR6 by small interfering RNA (siRNA) and neutralizing antibody inhibited IL‐17‐induced cell migration. By using specific inhibitors and short hairpin RNA (shRNA), we demonstrated that the activation of ERK and p38 pathways are critical for IL‐17‐induced CCR6 e...
Source: Journal of Cellular Physiology - September 9, 2014 Category: Cytology Authors: Chih‐Chien Chin, Cheng‐Nan Chen, Hsing‐Chun Kuo, Chung‐Sheng Shi, Meng Chiao Hsieh, Yi‐Hung Kuo, Shui‐Yi Tung, Kam‐Fai Lee, Wen‐Shih Huang Tags: Original Research Article Source Type: research
Regulation of Cigarette Smoke Induction of IL‐8 in Macrophages by AMP‐activated Protein Kinase Signaling
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Source: Journal of Cellular Physiology - December 11, 2014 Category: Cytology Authors: Hsin‐Kuo Ko, Hung‐Fu Lee, An‐Hsuan Lin, Meng‐Han Liu, Ching‐I Liu, Tzong‐Shyuan Lee, Yu Ru Kou Tags: Original Research Article Source Type: research
Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells through TGFβ1‐mediated Upregulation of Periostin Expression
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Source: Journal of Cellular Physiology - February 5, 2015 Category: Cytology Authors: Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Satoshi Monnouchi, Sayuri Hamano, Asuka Yuda, Akifumi Akamine Tags: Original Research Article Source Type: research
The Role of Maternal Gestational Diabetes in Inducing Fetal Endothelial Dysfunction
This study examines the effect of maternal diabetes on fetal endothelial function and gene expression under physiological glucose conditions (5mM). Human umbilical vein endothelial cell (HUVEC) isolated from diabetic mothers (d.HUVEC) grew more slowly than HUVEC isolated from healthy mothers (c.HUVEC) and had delayed doubling time despite increased levels of total vascular endothelial growth factor (VEGF) expression and protein production as determined by real time PCR and ELISA respectively. Using western blot, the levels of antiproliferative VEGF165b isoform were increased in d.HUVEC relative to c.HUVEC. Successful VEGF1...
Source: Journal of Cellular Physiology - March 24, 2015 Category: Cytology Authors: Samar A Sultan, Wanting Liu, Yonghong Peng, W. Roberts, Donald Whitelaw, Anne M Graham Tags: Original Research Article Source Type: research
CD44 Influences Fibroblast Behaviors Via Modulation of Cell‐Cell and Cell‐Matrix Interactions, Affecting Survivin and Hippo Pathways
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Source: Journal of Cellular Physiology - August 6, 2015 Category: Cytology Authors: Masayuki Tsuneki, Joseph A. Madri Tags: Original Research Article Source Type: research
Acetylcholine Attenuates Hypoxia/Reoxygenation Injury by Inducing Mitophagy through PINK1/Parkin Signal Pathway in H9c2 Cells
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Physiology - October 14, 2015 Category: Cytology Authors: Lei Sun, Mei Zhao, Yang Yang, Run‐Qing Xue, Xiao‐Jiang Yu, Jian‐Kang Liu, Wei‐Jin Zang Tags: Original Research Article Source Type: research
