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Source: Acta Biochimica et Biophysica Sinica
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Total 83 results found since Jan 2013.
LncRNA AP000695.2 promotes glycolysis of lung adenocarcinoma via the miR-335-3p/TEAD1 axis
This study aims to verify and explore the mechanism of AP000695.2 in glycolysis of LUAD. Overexpression plasmid and siRNA are used to construct cell models of upregulation and downregulation of AP000695.2, respectively. AP000695.2 is highly expressed in lung cancer cell lines as revealed by qPCR. Western blot analysis, FDG uptake, lactate production assay and ECAR determination results show that high expression of AP000695.2 facilitates glycolysis of LUAD cells. CCK-8, EdU staining, Transwell and wound healing assays show that high expression of AP000695.2 promotes cell growth and migration of LUAD. The relationship betwee...
Source: Acta Biochimica et Biophysica Sinica - September 19, 2023 Category: Biochemistry Authors: Shuoyan Xu Zhiming Cheng Bulin Du Yao Diao Yaming Li Xuena Li Source Type: research
ox-LDL induces autophagy-mediated apoptosis by suppressing secretagogin-regulated autophagic flux in pancreatic β-cells
In this study, we aim to explore the role of secretagogin (SCGN) in lipotoxicity-induced β-cell injury. Our results indicate that ox-LDL treatment leads to autophagic cell death, as evidenced by decreased cell viability, aggravated cell apoptosis, and the accumulation of the p62 protein in MIN6 cells. LysoTracker Red staining, TEM and mRFP-GFP-LC3 assays demonstrate that autophagic flux is blocked in ox-LDL-treated MIN6 cells. Intriguingly, SCGN is significantly decreased in MIN6 cells under lipotoxic conditions. Additionally, siRNA-guided SCGN knockdown blocks autophagic flux triggered by rapamycin, while SCGN restoratio...
Source: Acta Biochimica et Biophysica Sinica - February 15, 2023 Category: Biochemistry Authors: Ying Lv Sijie Xiao Shuhui Ouyang Zhengliang Peng Li Wu Ziqing Tang Weizheng Zhang Renxian Cao Jing Yang Source Type: research
Caveolin-1-deficient fibroblasts promote migration, invasion, and stemness via activating the TGF- β/Smad signaling pathway in breast cancer cells
This study refreshingly points out that CAV-1 deficiency in fibroblasts enhances TGF-β1 secretion and then activates the TGF-β1/Smad signaling pathway of BCCs, thus promoting the metastasis and stemness of BCCs. Collectively, our findings indicate an unexpected role of CAV-1 deficiency in fibroblasts and the tumor microenvironment as a permissive factor, which is regulated by the TGF-β1 signaling pathway in BCCs.PMID:36604141 | DOI:10.3724/abbs.2022150
Source: Acta Biochimica et Biophysica Sinica - January 5, 2023 Category: Biochemistry Authors: Qingyun Huang Longyuan Wu Yi Wang Xinyu Kong Xinhua Xiao Qiyuan Huang Miao Li Yujia Zhai Fuxiu Shi Ruichen Zhao Junpei Zhong Lixia Xiong Source Type: research
Induction of the ER stress response in NRVMs is linked to cardiotoxicity caused by celastrol
In this study, we explore celastrol's cardiotoxicity using the methods of histology and cell biology. The results show that celastrol administration dose-dependently induces cardiac dysfunction in mice as manifested by left ventricular dilation, myocardial interstitial fibrosis, and cardiomyocyte hypertrophy. Exposure to celastrol greatly decreases neonatal rat ventricular myocyte (NRVM) viability and promotes its apoptosis. More importantly, we demonstrate that celastrol exerts its pro-apoptotic effects through endoplasmic reticulum (ER) stress and unfolded protein response. Furthermore, siRNA targeting C/EBP homologous p...
Source: Acta Biochimica et Biophysica Sinica - August 19, 2022 Category: Biochemistry Authors: Zhong Chen Zhong Zhuang Chen Meng Zhonghua Zhu Yin Zhang Zhao Zhang Source Type: research
