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Source: Acta Biochimica et Biophysica Sinica

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Total 83 results found since Jan 2013.

siRNA of DNA polymerase iota inhibits the migration and invasion in the lung cancer cell A549.
Abstract DNA polymerase iota (polɩ) is a member of low-fidelity Y-family of DNA polymerases. Our previous studies have demonstrated that the overexpression of polι is associated with the poorer prognosis in lung cancer patients. Here, we designed the small interfering RNA (siRNA) targeting polɩ gene (POLI) to investigate the effect of polɩ on the proliferation, apoptosis, and invasion of the lung cancer cell line A549 in order to reveal the role of polι in lung cancer progression. Our results showed that siRNA of POLI had no significant effect on the proliferation and apoptosis of the lung cancer cell line A5...
Source: Acta Biochimica et Biophysica Sinica - July 27, 2018 Category: Biochemistry Authors: Li L, Tian H, Cheng C, Li S, Ming L, Qi L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Adenovirus-mediated delivery of siRNA targeting TM4SF4 attenuated liver cancer cell growth in vitro and in vivo.
In this study, we developed an adenoviral vector harboring an effective siRNA targeting TM4SF4 (AdSiTM4SF4) and identified its function in suppression of tumor cell growth. It was confirmed that TM4SF4 was overexpressed in HCC tissues compared with its paired non-tumor tissues by western blot analysis and immunohistochemistry. Remarkably, it was more abundant on the cell surface of HCC cells. The signals of ectopically expressed TM4SF4 in four cell lines dramatically localized in the plasma membrane, slightly in the cytoplasm, and absent in the nucleus, demonstrating that TM4SF4 is a membrane protein. Targeting TM4SF4 by A...
Source: Acta Biochimica et Biophysica Sinica - January 7, 2013 Category: Biochemistry Authors: Wang L, Feng J, Da L, Li Y, Li Z, Zhao M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

siRNA targeting stathmin inhibits invasion and enhances chemotherapy sensitivity of stem cells derived from glioma cell lines.
Abstract Glioma is one of the most highly angiogenic tumors, and glioma stem cells (GSCs) are responsible for resistance to chemotherapy and radiotherapy, as well as recurrence after operation. Stathmin is substantial for mitosis and plays an important role in proliferation and migration of glioma-derived endothelial cells. However, the relationship between stathmin and GSCs is incompletely understood. Here we isolated GSCs from glioma cell lines U87MG and U251, and then used siRNA targeting stathmin for silencing. We showed that silencing of stathmin suppressed the proliferation, increased the apoptosis rate, and...
Source: Acta Biochimica et Biophysica Sinica - October 27, 2014 Category: Biochemistry Authors: Song Y, Mu L, Han X, Liu X, Fu S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.
Abstract Simultaneous silencing of multiple upregulated genes is an attractive and viable treatment strategy for many incurable diseases including cancer. Herein we used a dual gene-targeted siRNA conjugate composed of VEGF and Survivin siRNA sequences in the same backbone to inhibit proliferation and angiogenesis in two human osteosarcoma cell lines. We synthesized siRNA sequences targeting the VEGF and Survivin genes individually (VEGF siRNA and Survivin siRNA) or simultaneously (one-chain-double-target siRNA: dual siRNA). VEGF and Survivin mRNA and protein expression levels in human osteosarcoma MG-63 and Saos-...
Source: Acta Biochimica et Biophysica Sinica - December 19, 2018 Category: Biochemistry Authors: Gu J, Ji Z, Li D, Dong Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

RNA interference targeting CD147 inhibits metastasis and invasion of human breast cancer MCF-7 cells by downregulating MMP-9/VEGF expression.
In this study, we investigated the role of CD147 in human breast cancer metastasis and invasion, and examined its underlying molecular mechanisms. Immunohistochemistry results revealed high expression of CD147 in human breast tumor tissues, which was positively correlated with the malignancy of breast cancer. MCF-7 cells were transfected with CD147 siRNA eukaryotic expression vector, which resulted in significant knockdown of CD147. We found that CD147 siRNA dramatically inhibited cell proliferation, metastasis, and invasion. Furthermore, our results demonstrated that CD147 siRNA inhibited the synthesis of matrix metallopr...
Source: Acta Biochimica et Biophysica Sinica - June 12, 2018 Category: Biochemistry Authors: Li F, Zhang J, Guo J, Jia Y, Han Y, Wang Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

PTPα-mediated Src activation by EGF in human breast cancer cells.
Abstract Protein tyrosine phosphatase alpha (PTPα) functions as an activator of Src by dephosphorylating Tyr527/530, a critical negative regulatory site. The increase of PTPα catalytic activity requires its phosphorylation at Ser180 and/or Ser204 and its dissociation from PTPα/Grb2 complex. Here, we show that epidermal growth factor (EGF) stimulation increases the ability of PTPα to activate Src by dephosphorylating Tyr530 in BT-20 and SKBR3 breast cancer cell lines. Treatment of these cells with EGF transiently decreased the association of PTPα with Grb2 and enhanced PTPα catalytic activity via Ser180 and S...
Source: Acta Biochimica et Biophysica Sinica - April 1, 2013 Category: Biochemistry Authors: Wang J, Yu L, Zheng X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-κB pathway.
Abstract Metastatic melanoma is one of the most deadly and evasive cancers. Collagen I in the extracellular matrix promotes the migration and invasion of tumor cells through the production of matrix metalloproteinase (MMP) 2 and 9. Discoidin domain receptor (DDR) 2 is a collagen receptor that is implicated in several cancer types including breast and prostate cancers. However, the role of DDR2 in the migration and invasion of murine melanoma cells is less studied. In the present study, we investigated the effects and underlying mechanisms of DDR2 in migration and invasion of B16BL6 melanoma cells in response to co...
Source: Acta Biochimica et Biophysica Sinica - March 1, 2015 Category: Biochemistry Authors: Poudel B, Lee YM, Kim DK Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Hypoxia promotes bone marrow-derived mesenchymal stem cell proliferation through apelin/APJ/autophagy pathway.
Abstract Bone marrow-derived mesenchymal stem cells (BMSCs) are a population of multipotent progenitors that have the capacity of proliferation and differentiation into mesenchymal lineage cells. The regulatory peptide apelin is the endogenous ligand for the G protein-coupled receptor APJ. Apelin, which can enhance BMSC proliferation, has mitogenic effects on a wide variety of cell types. We hypothesized that the increased apelin/APJ might be involved in the occurrence and development of hypoxia-induced BMSC proliferation. BMSCs from the bone marrow of 8- to 10-week-old C57BL/6J mice were cultured under either nor...
Source: Acta Biochimica et Biophysica Sinica - March 2, 2015 Category: Biochemistry Authors: Li L, Li L, Zhang Z, Jiang Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

The histone acetylation mediated by Gcn5 regulates the Hoxc11 gene expression in MEFs.
In this study, we propose to investigate the role of Gcn5, a histone acetyltransferase, in the regulation of Hoxc11 expression in MEFs. We showed that the H3 lysine 9 acetylation (H3K9ac) status has the same correlation with Hoxc11 expression and reported that Gcn5 is associated with the upregulation of Hoxc11 expression through H3K9ac in Akt1-/- MEFs. Since Hoxc11 was upregulated through histone acetylation in Akt1-/- MEFs, a functional role of Gcn5 on Hoxc11 expression was analyzed in Akt1-/- MEFs treated with Gcn5 specific inhibitor or transfected with Gcn5-small interfering RNA (Gcn5-siRNA). When the expression of Hoxc...
Source: Acta Biochimica et Biophysica Sinica - May 24, 2017 Category: Biochemistry Authors: Oh JH, Lee JY, Kong KA, Kim JM, Kim MH Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Opposite effects of Drosophila C3PO on gene silencing mediated by esi-2.1 and miRNA-bantam.
Abstract Translin/TRAX complex, also named as C3PO, is evolutionarily conserved and participates in diverse cellular processes in different organisms from yeast to human. C3PO plays a critical role in the activation of RNA-induced silencing complexes by promoting the unwinding and degradation of passenger strand of exogenous siRNAs (exo-siRNAs) in Drosophila and human. Moreover, human C3PO (hC3PO) has been found to broadly repress miRNAs by degrading miRNA precursors. However, the effect of Drosophila melanogaster C3PO (dmC3PO) on endogenous siRNA (endo-siRNA) and miRNA pathways remains unknown. Here, we found tha...
Source: Acta Biochimica et Biophysica Sinica - December 20, 2018 Category: Biochemistry Authors: Liu Y, Fang Y, Liu Y, Wang Z, Lyu B, Hu Y, Zhou X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide- α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway
Acta Biochim Biophys Sin (Shanghai). 2021 Sep 8:gmab115. doi: 10.1093/abbs/gmab115. Online ahead of print.ABSTRACTPhotodynamic therapy (PDT), which is a new method for treating tumors, has been used in the treatment of cancer. In-depth research has shown that PDT cannot completely kill tumor cells, indicating that tumor cells are resistant to PDT. Glucose regulatory protein 78 (GRP78), which is a key regulator of endoplasmic reticulum stress, has been confirmed to be related to tumor resistance and recurrence, but there are relatively few studies on the further mechanism of GRP78 in PDT. Our experiment aimed to observe the...
Source: Acta Biochimica et Biophysica Sinica - September 8, 2021 Category: Biochemistry Authors: Qiang Zuo Yunsheng Ou Shenxi Zhong Haoyang Yu Fangbiao Zhan Muzi Zhang Source Type: research

Macrophage-activating lipopeptide-2 downregulates the expression of ATP-binding cassette transporter A1 by activating the TLR2/NF-кB/ZNF202 pathway in THP-1 macrophages.
Abstract Macrophage-activating lipopeptide-2 (MALP-2) has been shown to promote the development of atherosclerosis. ATP-binding cassette transporter A1 (ABCA1), a transmembrane protein, plays a critical role in mediating cholesterol export from macrophages to apolipoprotein A-I (apoA-I). However, whether MALP-2 can regulate the expression of ABCA1 is still largely unknown. The aim of this study was to explore the effects of MALP-2 on ABCA1 expression in THP-1 macrophages and the underlying mechanisms. Our results showed that the treatment of cells with MALP-2 decreased ABCA1 level and suppressed cholesterol efflux...
Source: Acta Biochimica et Biophysica Sinica - February 27, 2016 Category: Biochemistry Authors: Peng L, Zhang Z, Zhang M, Yu X, Yao F, Tan Y, Liu D, Gong D, Chong H, Liu X, Zheng X, Tian G, Tang C Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Mixed lineage kinase domain-like protein induces RGC-5 necroptosis following elevated hydrostatic pressure.
Abstract Receptor-interacting protein 3 (RIP3) is an essential component of the necroptosis signaling pathway. Phosphorylation of its downstream target, mixed lineage kinase domain-like protein (MLKL), has been proposed to induce necroptosis by initiating Ca2+ influx. Our previous studies have shown that RGC-5 retinal ganglion cells undergo RIP3-mediated necroptosis following elevated hydrostatic pressure (EHP). However, the molecular mechanism underlying necroptosis induction downstream of RIP3 is still not well understood. Here, we investigated the effects of MLKL during EHP-induced necroptosis, and primarily ex...
Source: Acta Biochimica et Biophysica Sinica - October 1, 2017 Category: Biochemistry Authors: Liao L, Shang L, Li N, Wang S, Wang M, Huang Y, Chen D, Huang J, Xiong K Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research