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Silence of bFGF enhances chemosensitivity of glioma cells to temozolomide through the MAPK signal pathway.
In this study, we analyzed the relationship between bFGF and chemotherapy resistance, with the objective of offering new strategy for chemotherapy of glioma patients. Here, siRNA was used to silence the expression of bFGF in glioma cell lines including U87 and U251 followed by chemotherapy of temozolomide (TMZ). Then, the characters of glioma including proliferation, apoptosis, migration, and cell cycle were studied in U87 and U251 cell lines. Our results demonstrated that silencing bFGF enhanced the effect of TMZ by inhibiting proliferation and migration, blocking cell cycle in G0/G1, and promoting apoptosis. In addition,...
Source: Acta Biochimica et Biophysica Sinica - May 16, 2016 Category: Biochemistry Authors: Wang Q, Du J, Xu B, Xu L, Wang X, Liu J, Wang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy.
Abstract Diabetic retinopathy (DR) is the leading cause of new-onset blindness. The roles of microRNAs in diabetic retinopathy are largely unknown. The aim of this study is to investigate the role of miR-20b in DR. Transfection of miR-20b mimic in high glucose (HG)-treated human retinal endothelial cells (HRECs) increased miR-20b expression and decreased the expression level of VEGF mRNA, while transfection of miR-20b inhibitor in control HRECs reduced the miR-20b expression with a corresponding increase of VEGF mRNA. In vitro functional assay showed that transfection of miR-20b mimic prevented HG-induced increase...
Source: Acta Biochimica et Biophysica Sinica - July 13, 2016 Category: Biochemistry Authors: Qin B, Liu J, Liu S, Li B, Ren J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

FoxM1 inhibition enhances chemosensitivity of docetaxel-resistant A549 cells to docetaxel via activation of JNK/mitochondrial pathway.
Abstract Docetaxel is recommended as a second-line chemotherapy agent for the non-small-cell lung cancer (NSCLC); however, drug resistance greatly limits its efficiency. Forkhead box M1 (FoxM1), an oncogenic transcription factor, is believed to be involved in the chemoresistance of various human cancers; whereas the association of FoxM1 with acquired docetaxel-resistance in NSCLC remains unclear. In the present study, we investigated the involvement of FoxM1 in the docetaxel-resistant human lung adenocarcinoma A549 cells (A549/DTX). Our results showed that FoxM1 expression was significantly increased in the A549/D...
Source: Acta Biochimica et Biophysica Sinica - August 11, 2016 Category: Biochemistry Authors: Wang K, Zhu X, Zhang K, Zhu L, Zhou F Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Adenosine monophosphate-activated protein kinase attenuates cardiomyocyte hypertrophy through regulation of FOXO3a/MAFbx signaling pathway.
In this study, we tested the hypothesis that adenosine monophosphate-activated protein kinase (AMPK) activation attenuates cardiomyocyte hypertrophy by regulating FOXO3a/MAFbx signaling pathway and its downstream protein degradation. The results showed that activation of AMPK with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) attenuated cardiomyocyte hypertrophy induced by angiotensin II (Ang II). The antihypertrophic effects of AICAR were blunted by AMPK inhibitor Compound C. In addition, AMPK dramatically increased the activity of transcription factor FOXO3a, up-regulated the expression of its downstream ubiquiti...
Source: Acta Biochimica et Biophysica Sinica - August 11, 2016 Category: Biochemistry Authors: Chen B, Wu Q, Xiong Z, Ma Y, Yu S, Chen D, Huang S, Dong Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Activation of liver X receptors promotes inflammatory cytokine mRNA degradation by upregulation of tristetraprolin.
Abstract Liver X receptors (LXRs) have anti-inflammatory properties. Whether LXRs play a role in post-transcriptional control of inflammatory cytokine expression is not clear. Here, we firstly identified that the synthetic LXR agonist T0901317 promoted IL-1β, IL-6 and TNFα mRNA degradation. Moreover, T0901317 destabilized TNFα mRNA through its 3'-untranslated region. In addition, T0901317 increased the expression of tristetraprolin (TTP), while antagonizing TTP with siRNA abrogated T0901317-mediated inflammatory cytokine mRNA decay. Interestingly, T0901317 repressed LPS-induced phosphorylation of ERK1/2 and p38...
Source: Acta Biochimica et Biophysica Sinica - January 22, 2017 Category: Biochemistry Authors: Xiao J, Chen Q, Tang D, Ou W, Wang J, Mo Z, Tang C, Peng L, Wang D Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

miR-219a-5p inhibits breast cancer cell migration and epithelial-mesenchymal transition by targeting myocardin-related transcription factor A.
In conclusion, our present study revealed the tumor suppressive role of miR-219a-5p in regulating breast cancer migration by targeting MRTF-A, suggesting that miR-219a-5p might be a therapeutic target in breast cancer through regulating EMT. PMID: 29077787 [PubMed - as supplied by publisher]
Source: Acta Biochimica et Biophysica Sinica - October 25, 2017 Category: Biochemistry Authors: Zhuang C, Yuan Y, Song T, Wang H, Huang L, Luo X, He H, Huo L, Zhou H, Wang N, Zhang T Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

PKC inhibition of sotrastaurin has antitumor activity in diffuse large B-cell lymphoma via regulating the expression of MCT-1.
In this study, we found that the mRNA and protein expression levels of MCT-1 were visibly decreased after knocking down PKC by siRNA in SUDHL-4 and OCI-LY8 DLBCL cell lines. A selective PKC inhibitor, sotrastaurin, effectively inhibited cell proliferation and induced cell apoptosis in a dose- and time-dependent manner. Meanwhile, we also observed that the cell cycle was arrested in the G1 phase in sotrastaurin-treated cells. In addition, MCT-1 was down-regulated in the sotrastaurin treatment group in vivo. Furthermore, we demonstrated that the PKC inhibitor sotrastaurin induced cell apoptosis and cell cycle arrest in DLBCL...
Source: Acta Biochimica et Biophysica Sinica - March 9, 2018 Category: Biochemistry Authors: Chang G, Zheng J, Xiao W, Chang S, Wei Q, Wu H, Tao Y, Yang G, Xie B, Lan X, Wang Y, Yu D, Hu L, Xie Y, Bu W, Kong Y, Dai B, Hou J, Shi J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Integrin β3 inhibits hypoxia-induced apoptosis in cardiomyocytes.
Abstract Hypoxia-induced apoptosis plays an important role in cardiovascular diseases. Integrin β3 is one of the main integrin heterodimer receptors on the surface of cardiac myocytes. However, despite the important role that integrin β3 plays in the cardiovascular disease, its exact role in the hypoxia response remains unclear. Hence, in the present investigation we aimed to study the role of integrin β3 in hypoxia-induced apoptosis in H9C2 cells and primary rat myocardial cells. MTT assay, flow cytometry and TUNEL assay results showed that hypoxia inhibited cardiomyocyte proliferation and induced cardiomyocyt...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2018 Category: Biochemistry Authors: Su Y, Tian H, Wei L, Fu G, Sun T Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Prox1 is essential for oligodendrocyte survival and regulates oligodendrocyte apoptosis via the regulation of NOXA.
Abstract Demyelinating diseases, such as multiple sclerosis, are known to result from acute or chronic injury to the myelin sheath and inadequate remyelination. Its underlying molecular mechanisms, however, remain unclear. The transcription factor prospero homeobox 1 (Prox1) plays an essential role during embryonic development of the central nervous system and cell differentiation. Thus, we aimed to investigate the role of Prox1 in the survival and differentiation of oligodendrocytes. Cell viability was measured by MTT assay. Flow cytometry was conducted to analyze cell apoptosis. Ectopic-Prox1 and shProx1 were us...
Source: Acta Biochimica et Biophysica Sinica - June 20, 2018 Category: Biochemistry Authors: Chang W, Teng J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

PIM1 overexpression in T-cell lymphomas protects tumor cells from apoptosis and confers doxorubicin resistance by upregulating c-myc expression.
In this study, we studied the roles of PIM1 in established T-lymphoma cell lines Jurkat and HUT-78. CCK-8 assay was conducted to evaluate cell survival and flow cytometry was performed to evaluate cell death of TCL cells. siRNAs were used to knockdown the expression of PIM1 and c-myc. qRT-PCR was used to evaluate the mRNA expression levels of c-myc and PIM1. Western blot analysis was used to evaluate the protein expression levels of PIM1, c-myc, STAT3, and phospho-STAT3. Doxorubicin was used to determine the effect of PIM1 on apoptosis. Our results showed that PIM1 expression was markedly enhanced and induced c-myc express...
Source: Acta Biochimica et Biophysica Sinica - July 17, 2018 Category: Biochemistry Authors: Xiang X, Yuan D, Liu Y, Li J, Wen Q, Kong P, Gao L, Zhang C, Gao L, Peng X, Zhang X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

High-mobility group box 1 protein contributes to the immunogenicity of rTcdB-treated CT26 cells.
In this study, HMGB1 was found to be released from the rTcdB-treated CT26 cells. HMGB1 knockdown by using specific siRNA weakened the capacity of the BMDCs loaded with the rTcdB-treated CT26 cells to prime T cells in vitro and in vivo. The released HMGB1 from CT26 cells could interact with the receptor TLR4, which is closely related to DC activation and immune responses. The knockdown of HMGB1 also affected the phagocytosis of the rTcdB-treated CT26 cells by DCs in vitro. Furthermore, HMGB1 weakened the antitumor immunity of the rTcdB-treated CT26 cells, which protects mice from rechallenge of the live CT26 cells. Taken to...
Source: Acta Biochimica et Biophysica Sinica - July 20, 2018 Category: Biochemistry Authors: Gu H, Liu J, Chen S, Qi H, Shi K, Li S, Ma Y, Wang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Knockdown of LGALS12 inhibits porcine adipocyte adipogenesis via PKA-Erk1/2 signaling pathway.
In this study, the effects of LGALS12 on fat deposition were explored with primary culture of porcine SC and IM adipocytes. Analysis of LGALS12 expression across different tissues revealed that LGALS12 was predominantly expressed in adipose tissue. The LGALS12 expression patterns across stages of adipocyte differentiation were also evaluated, with differences observed between SC and IM fat. Small interfering RNA (siRNA) of LGALS12 was designed and transfected into porcine adipocytes derived from SC and IM fat. After transfection, the expression level of LGALS12 was significantly reduced, and the number of lipid droplets wa...
Source: Acta Biochimica et Biophysica Sinica - August 25, 2018 Category: Biochemistry Authors: Wu W, Yin Y, Xu K, Peng Y, Zhang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

LncRNA HOTAIR mediates TGF- β2-induced cell growth and epithelial-mesenchymal transition in human lens epithelial cells.
In this study, we confirmed that TGF-β2 induces EMT in SRA01/04 cells via the up-regulation of the long non-coding RNA (lncRNA) HOTAIR. To study the effects of HOTAIR on the proliferation, migration and EMT of SRA01/04 cells as well as the underlying mechanism, we used small interfering RNA (siRNA) to specifically attenuate HOTAIR expression in SRA01/04 cells. CCK8 cell-counting kit was used to examine SRA01/04 cell viability; EdU cell proliferation kit was used to examine SRA01/04 cell proliferation; Transwell system and scratch assays were used to observe cell migration; and qPCR and western blot analysis were used to e...
Source: Acta Biochimica et Biophysica Sinica - September 20, 2018 Category: Biochemistry Authors: Zhang Z, Zhu H, Liu Y, Quan F, Zhang X, Yu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Cell-free DNA derived from cancer cells facilitates tumor malignancy through Toll-like receptor 9 signaling-triggered interleukin-8 secretion in colorectal cancer.
Abstract Circulating cell-free DNA (cfDNA) has become a potential diagnostic and prognostic biomarker for colorectal cancer (CRC). In non-cancerous diseases, it has been confirmed that cfDNA can be recognized by Toll-like receptor 9 (TLR9), leading to a significant biological change. Nevertheless, the biological significance of cfDNA and its relationship with TLR9 in tumor malignancy is still unclear. Therefore, the purpose of this study is to explore the biological role of cfDNA in colorectal cancer (CRC). The expression of TLR9 was measured in different CRC cell lines and cancerous samples by RT-PCR or immunohis...
Source: Acta Biochimica et Biophysica Sinica - September 15, 2018 Category: Biochemistry Authors: Niu Z, Tang W, Liu T, Xu P, Zhu D, Ji M, Huang W, Ren L, Wei Y, Xu J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

YAP mediates the positive regulation of hnRNPK on the lung adenocarcinoma H1299 cell growth.
In this study, we observed that the expression pattern of hnRNPK in H1299 lung adenocarcinoma cells varied depending on the cell density in culture. Moreover, hnRNPK stimulated the ability of proliferation and colony formation of H1299 cells, which is important for the multilayered cell growth in culture. We further investigated whether there is an association between hnRNPK and the elements involved in the cell contact inhibition pathway. By using quantitative reverse transcriptase-polymerase chain reaction assay and a YAP activity reporter system, we found that hnRNPK upregulated the mRNA and protein levels and transcrip...
Source: Acta Biochimica et Biophysica Sinica - June 11, 2019 Category: Biochemistry Authors: Xu L, Zhang T, Huang W, Liu X, Lu J, Gao X, Zhang YF, Liu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

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