LncRNA HOTAIR mediates TGF- β2-induced cell growth and epithelial-mesenchymal transition in human lens epithelial cells.

In this study, we confirmed that TGF-β2 induces EMT in SRA01/04 cells via the up-regulation of the long non-coding RNA (lncRNA) HOTAIR. To study the effects of HOTAIR on the proliferation, migration and EMT of SRA01/04 cells as well as the underlying mechanism, we used small interfering RNA (siRNA) to specifically attenuate HOTAIR expression in SRA01/04 cells. CCK8 cell-counting kit was used to examine SRA01/04 cell viability; EdU cell proliferation kit was used to examine SRA01/04 cell proliferation; Transwell system and scratch assays were used to observe cell migration; and qPCR and western blot analysis were used to evaluate EMT progression. We found that inhibition of HOTAIR expression repressed SRA01/04 cell viability, proliferation, migration and prevented the TGF-β2-induced changes in cellular processes via modulation of EMT. Ultimately, we found that HOTAIR affected the TGF-β/Smad signaling pathway. In summary, we elucidated that HOTAIR affected the cell viability, proliferation, and migration in the TGF-β2-induced EMT in SRA01/04 cells and suggested that modulation of HOTAIR may be helpful in PCO prevention and therapy.
PMID: 30239553 [PubMed - as supplied by publisher]

Source: Acta Biochimica et Biophysica Sinica - September 20, 2018 Category: Biochemistry Authors: Zhang Z, Zhu H, Liu Y, Quan F, Zhang X, Yu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research