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Source: Acta Biochimica et Biophysica Sinica

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Total 83 results found since Jan 2013.

Voltage-gated sodium channel Nav1.5 promotes proliferation, migration and invasion of oral squamous cell carcinoma.
In this study, we found that Nav1.5 was highly expressed in OSCC cell lines compared with normal oral keratinocyte HOK cell line by using western blot analysis. CCK-8 assay results revealed that downregulation of Nav1.5 expression by its specific siRNA reduced proliferation of OSCC HSC-3 cells. Moreover, transwell assay results showed Nav1.5 knockdown significantly inhibited migration and invasion of HSC-3 cells. Meanwhile, qRT-PCR and western blot analysis results showed that epidermal growth factor (EGF) induced Nav1.5 expression in a time- and dose-dependent manner. In addition, EGF promoted proliferation, migration and...
Source: Acta Biochimica et Biophysica Sinica - May 28, 2019 Category: Biochemistry Authors: Zhang J, Mao W, Dai Y, Qian C, Dong Y, Chen Z, Meng L, Jiang Z, Huang T, Hu J, Luo P, Korner H, Jiang Y, Ying S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Downregulation of long non-coding RNA MEG3 promotes proliferation, migration, and invasion of human hepatocellular carcinoma cells by upregulating TGF- β1.
In this study, we aimed to investigate the role of lncRNA maternally expressed gene 3 (MEG3) in the development and progression of hepatocellular carcinoma. Expression of MEG3 in tumor tissues and adjacent healthy tissues of hepatocellular carcinoma patients, as well as the serum of both hepatocellular carcinoma patients and healthy controls, was detected by quantitative reverse transcriptase-polymerase chain reaction. The results showed that expression level of MEG3 was significantly lower in tumor tissues than in adjacent healthy tissues. Serum level of MEG3 was also significantly lower in hepatocellular carcinoma patien...
Source: Acta Biochimica et Biophysica Sinica - May 13, 2019 Category: Biochemistry Authors: Dong H, Zhang Y, Xu Y, Ma R, Liu L, Luo C, Jiang W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Opposite effects of Drosophila C3PO on gene silencing mediated by esi-2.1 and miRNA-bantam.
Abstract Translin/TRAX complex, also named as C3PO, is evolutionarily conserved and participates in diverse cellular processes in different organisms from yeast to human. C3PO plays a critical role in the activation of RNA-induced silencing complexes by promoting the unwinding and degradation of passenger strand of exogenous siRNAs (exo-siRNAs) in Drosophila and human. Moreover, human C3PO (hC3PO) has been found to broadly repress miRNAs by degrading miRNA precursors. However, the effect of Drosophila melanogaster C3PO (dmC3PO) on endogenous siRNA (endo-siRNA) and miRNA pathways remains unknown. Here, we found tha...
Source: Acta Biochimica et Biophysica Sinica - December 20, 2018 Category: Biochemistry Authors: Liu Y, Fang Y, Liu Y, Wang Z, Lyu B, Hu Y, Zhou X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.
Abstract Simultaneous silencing of multiple upregulated genes is an attractive and viable treatment strategy for many incurable diseases including cancer. Herein we used a dual gene-targeted siRNA conjugate composed of VEGF and Survivin siRNA sequences in the same backbone to inhibit proliferation and angiogenesis in two human osteosarcoma cell lines. We synthesized siRNA sequences targeting the VEGF and Survivin genes individually (VEGF siRNA and Survivin siRNA) or simultaneously (one-chain-double-target siRNA: dual siRNA). VEGF and Survivin mRNA and protein expression levels in human osteosarcoma MG-63 and Saos-...
Source: Acta Biochimica et Biophysica Sinica - December 19, 2018 Category: Biochemistry Authors: Gu J, Ji Z, Li D, Dong Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Chronic intermittent hypoxia promotes the development of experimental non-alcoholic steatohepatitis by modulating Treg/Th17 differentiation.
Abstract The present study aims to characterize the effect of chronic intermittent hypoxia and HIF1α on the non-alcoholic steatohepatitis (NASH) process in mice, and to explore the role of the Treg/Th17 balance in the formation of NASH inflammation and fibrosis. To achieve this purpose, simple steatosis was induced in mice by high-fat diet administration. Subsequently, chronic intermittent hypoxia was simulated by intraperitoneally injecting sodium nitrite. The changes of inflammation, fibrosis, and Treg/Th17 balance in the liver were quantified under chronic intermittent hypoxia condition and after tail vein inj...
Source: Acta Biochimica et Biophysica Sinica - October 31, 2018 Category: Biochemistry Authors: Liu J, Li W, Zhu W, He W, Zhao H, Xiang Y, Liu C, Wu W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

LncRNA HOTAIR mediates TGF- β2-induced cell growth and epithelial-mesenchymal transition in human lens epithelial cells.
In this study, we confirmed that TGF-β2 induces EMT in SRA01/04 cells via the up-regulation of the long non-coding RNA (lncRNA) HOTAIR. To study the effects of HOTAIR on the proliferation, migration and EMT of SRA01/04 cells as well as the underlying mechanism, we used small interfering RNA (siRNA) to specifically attenuate HOTAIR expression in SRA01/04 cells. CCK8 cell-counting kit was used to examine SRA01/04 cell viability; EdU cell proliferation kit was used to examine SRA01/04 cell proliferation; Transwell system and scratch assays were used to observe cell migration; and qPCR and western blot analysis were used to e...
Source: Acta Biochimica et Biophysica Sinica - September 20, 2018 Category: Biochemistry Authors: Zhang Z, Zhu H, Liu Y, Quan F, Zhang X, Yu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Cell-free DNA derived from cancer cells facilitates tumor malignancy through Toll-like receptor 9 signaling-triggered interleukin-8 secretion in colorectal cancer.
Abstract Circulating cell-free DNA (cfDNA) has become a potential diagnostic and prognostic biomarker for colorectal cancer (CRC). In non-cancerous diseases, it has been confirmed that cfDNA can be recognized by Toll-like receptor 9 (TLR9), leading to a significant biological change. Nevertheless, the biological significance of cfDNA and its relationship with TLR9 in tumor malignancy is still unclear. Therefore, the purpose of this study is to explore the biological role of cfDNA in colorectal cancer (CRC). The expression of TLR9 was measured in different CRC cell lines and cancerous samples by RT-PCR or immunohis...
Source: Acta Biochimica et Biophysica Sinica - September 15, 2018 Category: Biochemistry Authors: Niu Z, Tang W, Liu T, Xu P, Zhu D, Ji M, Huang W, Ren L, Wei Y, Xu J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Knockdown of LGALS12 inhibits porcine adipocyte adipogenesis via PKA-Erk1/2 signaling pathway.
In this study, the effects of LGALS12 on fat deposition were explored with primary culture of porcine SC and IM adipocytes. Analysis of LGALS12 expression across different tissues revealed that LGALS12 was predominantly expressed in adipose tissue. The LGALS12 expression patterns across stages of adipocyte differentiation were also evaluated, with differences observed between SC and IM fat. Small interfering RNA (siRNA) of LGALS12 was designed and transfected into porcine adipocytes derived from SC and IM fat. After transfection, the expression level of LGALS12 was significantly reduced, and the number of lipid droplets wa...
Source: Acta Biochimica et Biophysica Sinica - August 25, 2018 Category: Biochemistry Authors: Wu W, Yin Y, Xu K, Peng Y, Zhang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

LC3-II may mediate ATR-induced mitophagy in dopaminergic neurons through SQSTM1/p62 pathway.
Abstract Atrazine (2-chloro-4-ethylamino-6-isopropylamine-1,3,5-triazine; ATR) has been demonstrated to regulate autophagy- and apoptosis-related proteins in doparminergic neuronal damage. In our study, we investigated the role of LC3-II in ATR-induced degeneration of dopaminergic neurons. In vivo dopaminergic neuron degeneration model was set up with ATR treatment and confirmed by the behavioral responses and pathological analysis. Dopaminergic neuron cells were transfected with LC3-II siRNA and treated with ATR to observe cell survival and reactive oxygen species release. The process of mitochondrial autophagy a...
Source: Acta Biochimica et Biophysica Sinica - August 2, 2018 Category: Biochemistry Authors: Ma K, Wu H, Li P, Li B Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

siRNA of DNA polymerase iota inhibits the migration and invasion in the lung cancer cell A549.
Abstract DNA polymerase iota (polɩ) is a member of low-fidelity Y-family of DNA polymerases. Our previous studies have demonstrated that the overexpression of polι is associated with the poorer prognosis in lung cancer patients. Here, we designed the small interfering RNA (siRNA) targeting polɩ gene (POLI) to investigate the effect of polɩ on the proliferation, apoptosis, and invasion of the lung cancer cell line A549 in order to reveal the role of polι in lung cancer progression. Our results showed that siRNA of POLI had no significant effect on the proliferation and apoptosis of the lung cancer cell line A5...
Source: Acta Biochimica et Biophysica Sinica - July 27, 2018 Category: Biochemistry Authors: Li L, Tian H, Cheng C, Li S, Ming L, Qi L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

High-mobility group box 1 protein contributes to the immunogenicity of rTcdB-treated CT26 cells.
In this study, HMGB1 was found to be released from the rTcdB-treated CT26 cells. HMGB1 knockdown by using specific siRNA weakened the capacity of the BMDCs loaded with the rTcdB-treated CT26 cells to prime T cells in vitro and in vivo. The released HMGB1 from CT26 cells could interact with the receptor TLR4, which is closely related to DC activation and immune responses. The knockdown of HMGB1 also affected the phagocytosis of the rTcdB-treated CT26 cells by DCs in vitro. Furthermore, HMGB1 weakened the antitumor immunity of the rTcdB-treated CT26 cells, which protects mice from rechallenge of the live CT26 cells. Taken to...
Source: Acta Biochimica et Biophysica Sinica - July 20, 2018 Category: Biochemistry Authors: Gu H, Liu J, Chen S, Qi H, Shi K, Li S, Ma Y, Wang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

PIM1 overexpression in T-cell lymphomas protects tumor cells from apoptosis and confers doxorubicin resistance by upregulating c-myc expression.
In this study, we studied the roles of PIM1 in established T-lymphoma cell lines Jurkat and HUT-78. CCK-8 assay was conducted to evaluate cell survival and flow cytometry was performed to evaluate cell death of TCL cells. siRNAs were used to knockdown the expression of PIM1 and c-myc. qRT-PCR was used to evaluate the mRNA expression levels of c-myc and PIM1. Western blot analysis was used to evaluate the protein expression levels of PIM1, c-myc, STAT3, and phospho-STAT3. Doxorubicin was used to determine the effect of PIM1 on apoptosis. Our results showed that PIM1 expression was markedly enhanced and induced c-myc express...
Source: Acta Biochimica et Biophysica Sinica - July 17, 2018 Category: Biochemistry Authors: Xiang X, Yuan D, Liu Y, Li J, Wen Q, Kong P, Gao L, Zhang C, Gao L, Peng X, Zhang X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Prox1 is essential for oligodendrocyte survival and regulates oligodendrocyte apoptosis via the regulation of NOXA.
Abstract Demyelinating diseases, such as multiple sclerosis, are known to result from acute or chronic injury to the myelin sheath and inadequate remyelination. Its underlying molecular mechanisms, however, remain unclear. The transcription factor prospero homeobox 1 (Prox1) plays an essential role during embryonic development of the central nervous system and cell differentiation. Thus, we aimed to investigate the role of Prox1 in the survival and differentiation of oligodendrocytes. Cell viability was measured by MTT assay. Flow cytometry was conducted to analyze cell apoptosis. Ectopic-Prox1 and shProx1 were us...
Source: Acta Biochimica et Biophysica Sinica - June 20, 2018 Category: Biochemistry Authors: Chang W, Teng J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

RNA interference targeting CD147 inhibits metastasis and invasion of human breast cancer MCF-7 cells by downregulating MMP-9/VEGF expression.
In this study, we investigated the role of CD147 in human breast cancer metastasis and invasion, and examined its underlying molecular mechanisms. Immunohistochemistry results revealed high expression of CD147 in human breast tumor tissues, which was positively correlated with the malignancy of breast cancer. MCF-7 cells were transfected with CD147 siRNA eukaryotic expression vector, which resulted in significant knockdown of CD147. We found that CD147 siRNA dramatically inhibited cell proliferation, metastasis, and invasion. Furthermore, our results demonstrated that CD147 siRNA inhibited the synthesis of matrix metallopr...
Source: Acta Biochimica et Biophysica Sinica - June 12, 2018 Category: Biochemistry Authors: Li F, Zhang J, Guo J, Jia Y, Han Y, Wang Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Integrin β3 inhibits hypoxia-induced apoptosis in cardiomyocytes.
Abstract Hypoxia-induced apoptosis plays an important role in cardiovascular diseases. Integrin β3 is one of the main integrin heterodimer receptors on the surface of cardiac myocytes. However, despite the important role that integrin β3 plays in the cardiovascular disease, its exact role in the hypoxia response remains unclear. Hence, in the present investigation we aimed to study the role of integrin β3 in hypoxia-induced apoptosis in H9C2 cells and primary rat myocardial cells. MTT assay, flow cytometry and TUNEL assay results showed that hypoxia inhibited cardiomyocyte proliferation and induced cardiomyocyt...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2018 Category: Biochemistry Authors: Su Y, Tian H, Wei L, Fu G, Sun T Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research