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Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide- α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway
Acta Biochim Biophys Sin (Shanghai). 2021 Sep 8:gmab115. doi: 10.1093/abbs/gmab115. Online ahead of print.ABSTRACTPhotodynamic therapy (PDT), which is a new method for treating tumors, has been used in the treatment of cancer. In-depth research has shown that PDT cannot completely kill tumor cells, indicating that tumor cells are resistant to PDT. Glucose regulatory protein 78 (GRP78), which is a key regulator of endoplasmic reticulum stress, has been confirmed to be related to tumor resistance and recurrence, but there are relatively few studies on the further mechanism of GRP78 in PDT. Our experiment aimed to observe the...
Source: Acta Biochimica et Biophysica Sinica - September 8, 2021 Category: Biochemistry Authors: Qiang Zuo Yunsheng Ou Shenxi Zhong Haoyang Yu Fangbiao Zhan Muzi Zhang Source Type: research

Elevated ZIPK is required for TNF- α-induced cell adhesion molecule expression and leucocyte adhesion in endothelial cells
Acta Biochim Biophys Sin (Shanghai). 2021 Mar 12:gmab019. doi: 10.1093/abbs/gmab019. Online ahead of print.ABSTRACTLeucocyte adhesion to the vascular endothelium is a critical event in the early inflammatory response to infection and injury. This process is primarily regulated by the expression of cell adhesion molecules (CAMs) in endothelial cells. It has been well documented that tumor necrosis factor alpha (TNF-α) is a key regulator of CAM expression within this process, but its regulatory mechanism remains controversial. To investigate the scenario within this process, we assessed the role of zipper-interacting protei...
Source: Acta Biochimica et Biophysica Sinica - March 12, 2021 Category: Biochemistry Authors: Weiwei Zeng Zhiyuan Sun Tengxiang Ma Xiaobin Song Shuai Li Qianqian Zhang Wen Yuan Jing Li Li Liu Minsheng Zhu Huaqun Chen Source Type: research

Inhibition of lipogenesis and induction of apoptosis by valproic acid in prostate cancer cells via the C/EBP α/SREBP-1 pathway.
In this study, we aimed to investigate the effects and mechanisms of VPA on cell viability, lipogenesis, and apoptosis in human prostate cancer PC-3 and LNCaP cells. The results showed that VPA significantly reduced lipid accumulation and induced apoptosis of PC-3 and LNCaP cells. Moreover, the expression of CCAAT/enhancer-binding protein α (C/EBPα), as well as sterol regulatory element-binding protein 1 (SREBP-1) and its downstream effectors, including fatty acid synthase (FASN), acetyl CoA carboxylase 1 (ACC1), and anti-apoptotic B-cell lymphoma 2 (Bcl-2), was markedly decreased in PC-3 and LNCaP cells after VPA admini...
Source: Acta Biochimica et Biophysica Sinica - January 20, 2021 Category: Biochemistry Authors: Pang BO, Zhang J, Zhang XI, Yuan J, Shi Y, Qiao L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

A novel secretagogin/ATF4 pathway is involved in oxidized LDL-induced endoplasmic reticulum stress and islet β-cell apoptosis.
In this study, MIN6 cells were treated with oxidized low-density lipoprotein (ox-LDL) for 24 h, and then intracellular lipid droplets and cell apoptosis were quantified, and SCGN and ER stress markers were identified by western blot analysis. Furthermore, small interfer RNA (siRNA)-mediated SCGN knockdown and recombinant plasmid-mediated SCGN restoration experiments were performed to confirm the role of SCGN in ER stress and associated cell apoptosis. Finally, the interaction of SCGN with ATF4 was computationally predicted and then validated by a co-immunoprecipitation assay. We found that ox-LDL treatment increased the le...
Source: Acta Biochimica et Biophysica Sinica - December 8, 2020 Category: Biochemistry Authors: Wu L, Lv Y, Lv Y, Xiang S, Zhao Z, Tang Z, Ou L, Yan B, Xiao X, Wen G, Cao R, Yang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Long non-coding RNA LINC-PINT attenuates paclitaxel resistance in triple-negative breast cancer cells via targeting the RNA-binding protein NONO.
Abstract The treatment of triple-negative breast cancer (TNBC) relies largely on chemotherapies. However, it is frequent that TNBC patients develop resistance to the chemotherapy drugs. Generation of drug-resistant cell lines facilitates the identification of drug resistance. Here, we established two paclitaxel (PTX)-resistant TNBC cancer cell lines using an intermittent and stepwise method and found that long non-coding RNA long intergenic non-protein-coding RNA p53-induced transcript (LINC-PINT) was significantly decreased in PTX-resistant cancer cells. Ectopic expression of LINC-PINT sensitized both PTX-resista...
Source: Acta Biochimica et Biophysica Sinica - July 6, 2020 Category: Biochemistry Authors: Chen J, Zhu M, Zou L, Xia J, Huang J, Deng Q, Xu R Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

miR-487a performs oncogenic functions in osteosarcoma by targeting BTG2 mRNA.
In this study, we elucidated the involvement of miR-487a in OS and the underlying molecular mechanisms. We found that miR-487a was upregulated in OS clinical samples and cell lines. Knockdown of miR-487a suppressed OS cell growth and invasion and induced apoptosis; however, overexpression of miR-487a promoted OS cell growth and invasion. Accordingly, downregulation of miR-487a significantly suppressed tumor growth of OS xenografts in vivo. Furthermore, B-cell translocation gene 2 (BTG2) mRNA was found to be a novel target of miR-487a. Knockdown of BTG2 using small interfering RNA (siRNA) recapitulated the oncogenic effects...
Source: Acta Biochimica et Biophysica Sinica - May 12, 2020 Category: Biochemistry Authors: Gu Z, Wu S, Xu G, Wu W, Mao B, Zhao S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Nrf2/ARE pathway activation is involved in negatively regulating heat-induced apoptosis in non-small cell lung cancer cells.
In this study, using immunocytochemistry and western blot analysis, we demonstrated that heat stress induced Nrf2/ARE activation through the nuclear translocation of Nrf2 in non-small cell lung cancer cells. Luciferase activity was also increased. Additionally, antioxidant enzymes were increased through Nrf2 activation after heat stress. Transfection of lung cancer cells with siRNA directed against Nrf2 increased heat cytotoxicity and cell apoptosis. Heat stress could induce reactive oxygen species (ROS) accumulation, while the antioxidant NAC obviously reduced cell apoptosis ratio, indicating that heat stress induced cell...
Source: Acta Biochimica et Biophysica Sinica - April 6, 2020 Category: Biochemistry Authors: Xie W, Tan B, Yang Z, Yu X, Chen L, Ran D, Xu Q, Zhou X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

FoxM1 affects adhesive, migratory, and invasive abilities of human retinoblastoma Y-79 cells by targeting matrix metalloproteinase 2.
In this study, we aimed to investigate the correlation between FoxM1 and retinoblastoma (Rb) metastasis and to explore the detailed mechanism. Wound healing, cell adhesion, and invasion assays showed that FoxM1 overexpression induced epithelial-mesenchymal transition in Y-79 cells and inhibited adhesion and subsequently promoted metastasis of Y-79 cells, while FoxM1 knockdown showed the opposite effects. A luciferase reporter assay and chromatin immunoprecipitation assay provided evidence that FoxM1 promoted matrix metalloproteinase 2 (MMP2) transcription by directly binding to and promoting MMP2 promoter. MMP2 knockdown b...
Source: Acta Biochimica et Biophysica Sinica - March 9, 2020 Category: Biochemistry Authors: Zhu X, Yu M, Wang K, Zou W, Zhu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Resveratrol attenuates diabetes-associated cell centrosome amplification via inhibiting the PKC α-p38 to c-myc/c-jun pathway.
In conclusion, our results suggest that PKCα-p38 to c-myc/c-jun is the signaling pathway of the diabetes-associated centrosome amplification, and resveratrol attenuates the centrosome amplification by inhibiting this signaling pathway. PMID: 31844893 [PubMed - as supplied by publisher]
Source: Acta Biochimica et Biophysica Sinica - December 15, 2019 Category: Biochemistry Authors: Wu Q, Chen X, He Q, Lang L, Xu P, Wang P, Lee SC Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

MiR-122-5p suppresses the proliferation, migration, and invasion of gastric cancer cells by targeting LYN.
In this study, we found that miR-122-5p was low-expressed in GC tissues and cell lines by using qRT-PCR. Overexpression of miR-122-5p inhibited the proliferation, migration, and invasion of GC cells by using CCK-8 and transwell assays. On the contrary, downregulation of miR-122-5p promoted the proliferation, migration, and invasion of GC cells. In addition, we found that the expression of LYN, an Src family tyrosine kinase, was inversely correlated with miR-122-5p expression in GC tissues by using western blot analysis, immunohistochemistry, and qRT-PCR assays. Meanwhile, luciferase assay results indicated that LYN is a di...
Source: Acta Biochimica et Biophysica Sinica - December 10, 2019 Category: Biochemistry Authors: Meng L, Chen Z, Jiang Z, Huang T, Hu J, Luo P, Zhang H, Huang M, Huang L, Chen Y, Lu M, Xu AM, Ying S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Quercetin improves endothelial insulin sensitivity in obese mice by inhibiting Drp1 phosphorylation at serine 616 and mitochondrial fragmentation.
Abstract Studies have shown that endothelial insulin resistance induced by oxidative stress contributes to vascular dysfunction in metabolic disorders. Quercetin, a natural antioxidant, has been recently shown to exert protective effects on endothelial function. However, the effects of quercetin on endothelial insulin resistance and its underlying mechanism are unclear. Here, we found that chronic oral treatment of obese mice with quercetin increased vascular endothelial insulin sensitivity, accompanied by alleviated mitochondrial fragmentation as revealed by confocal imaging. In addition, western blot analysis sh...
Source: Acta Biochimica et Biophysica Sinica - November 27, 2019 Category: Biochemistry Authors: Chen C, Huang J, Shen J, Bai Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

YAP mediates the positive regulation of hnRNPK on the lung adenocarcinoma H1299 cell growth.
In this study, we observed that the expression pattern of hnRNPK in H1299 lung adenocarcinoma cells varied depending on the cell density in culture. Moreover, hnRNPK stimulated the ability of proliferation and colony formation of H1299 cells, which is important for the multilayered cell growth in culture. We further investigated whether there is an association between hnRNPK and the elements involved in the cell contact inhibition pathway. By using quantitative reverse transcriptase-polymerase chain reaction assay and a YAP activity reporter system, we found that hnRNPK upregulated the mRNA and protein levels and transcrip...
Source: Acta Biochimica et Biophysica Sinica - June 11, 2019 Category: Biochemistry Authors: Xu L, Zhang T, Huang W, Liu X, Lu J, Gao X, Zhang YF, Liu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

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