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Source: Am J Physiol Cell Ph...

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Total 155 results found since Jan 2013.

Reactive oxygen species up-regulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells.
K, Sokabe M, Nikawa T Abstract Unloading-mediated muscle atrophy is associated with increased reactive oxygen species (ROS) production. We previously demonstrated that elevated ubiquitin ligase casitas B-lineage lymphoma-b (Cbl-b) resulted in the loss of muscle volume [Nakao R. et al. Mol Cell Biol, 29: 4798-4811, 2009]. However, the pathological role of ROS production associated with unloading-mediated muscle atrophy still remains unknown. Here, we showed the ROS-mediated signal transduction caused by microgravity or its simulation contributes to Cbl-b expression. In L6 myotubes, the assessment of redox status re...
Source: Am J Physiol Cell Ph... - March 7, 2018 Category: Cytology Authors: Uchida T, Sakashita Y, Kitahata K, Yamashita Y, Tomida C, Kimori Y, Komatsu A, Hirasaka K, Ohno A, Nakao R, Higashitani A, Higashibata A, Ishioka N, Shimazu T, Kobayashi T, Okumura Y, Choi I, Oarada M, Mills EM, Teshima-Kondo S, Takeda S, Tanaka E, Tanaka Tags: Am J Physiol Cell Physiol Source Type: research

A2BAR activation attenuates acute lung injury by inhibiting alveolar epithelial cell apoptosis both in vivo and in vitro.
In conclusion, A2BAR activation effectively attenuated OA-induced ALI by inhibiting AEC apoptosis and mitigated H2O2-induced AEC injury by suppressing the p38 and ERK1/2-mediated mitochondrial apoptosis pathway. PMID: 29898376 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - June 13, 2018 Category: Cytology Authors: Xu X, Zhu Q, Niu F, Zhang R, Wang Y, Wang W, Sun D, Wang X, Wang A Tags: Am J Physiol Cell Physiol Source Type: research

Adenosinergic Signaling Inhibits Oxalate Transport by Human Intestinal Caco2-BBE Cells Through the A2B Adenosine Receptor.
Abstract Most kidney stones (KS) are composed of calcium oxalate, and small increases in urine oxalate affect the stone risk. Intestinal oxalate secretion mediated by anion exchanger SLC26A6 (PAT1) plays a crucial role in limiting net absorption of ingested oxalate; thereby preventing hyperoxaluria and related KS, reflecting the importance of understanding regulation of intestinal oxalate transport. We previously showed that ATP and UTP inhibit oxalate transport by human intestinal Caco2-BBE cells (C2). Since ATP is rapidly degraded to adenosine (ADO), we examined whether intestinal oxalate transport is regulated ...
Source: Am J Physiol Cell Ph... - July 18, 2018 Category: Cytology Authors: Jung D, Alshaikh A, Ratakonda S, Bashir M, Amin MR, Jeon S, Stevens J, Sharma S, Ahmed W, Musch M, Hassan H Tags: Am J Physiol Cell Physiol Source Type: research

USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway.
In conclusion, these results suggest that USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway. PMID: 30281322 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - October 3, 2018 Category: Cytology Authors: Chen Q, Hang Y, Zhang T, Tan L, Li S, Jin Y Tags: Am J Physiol Cell Physiol Source Type: research

Coagulation factor XI induces Ca2+ response and accelerates cell migration in vascular smooth muscle cells via proteinase-activated receptor 1.
In conclusion, FXIa mainly elicits Ca2+ signal via the PAR1/CaV1.2-mediated Ca2+ influx and accelerates the migration in vascular smooth muscle cells. The present study provides the first evidence that FXIa exerts a direct cellular effect on vascular smooth muscle. PMID: 30566391 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - December 19, 2018 Category: Cytology Authors: Liu W, Hashimoto T, Yamashita T, Hirano K Tags: Am J Physiol Cell Physiol Source Type: research

TRPV2 channels mediate insulin secretion induced by cell swelling in mouse pancreatic β-cells.
Abstract β-Cell swelling induces membrane depolarization, which has been suggested to be caused at least partly by the activation of cation channels. Here, we show the identification of the cation channels. In isolated mouse pancreatic β-cells, the exposure to 30% hypotonic solution elicited an increase in cytosolic Ca2+ concentration ([Ca2+]c). The [Ca2+]c elevation was partially inhibited by ruthenium red, a blocker of several Ca2+-permeable channels including transient receptor potential vanilloid receptors (TRPV), and by nicardipine, but not by the depletion of intracellular Ca2+ stores with thapsigargin and...
Source: Am J Physiol Cell Ph... - January 16, 2019 Category: Cytology Authors: Sawatani T, Kaneko YK, Doutsu I, Ogawa A, Ishikawa T Tags: Am J Physiol Cell Physiol Source Type: research

Mechanisms of Niemann Pick type C1 Like 1 Protein Degradation in Intestinal Epithelial Cells.
In conclusion, our results showed that basal level of intestinal cholesterol transporter NPC1L1 protein is modulated by both ubiquitin-proteasome and lysosome-dependent degradation as well as by ERK1/2-dependent pathway. The modulation of these pathways may provide novel clues for therapeutic intervention to inhibit cholesterol absorption and lower plasma cholesterol. PMID: 30789754 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - February 21, 2019 Category: Cytology Authors: Malhotra P, Soni V, Yamanashi Y, Takada T, Suzuki H, Gill RK, Saksena S, Dudeja PK, Alrefai WA Tags: Am J Physiol Cell Physiol Source Type: research

Identification of transmembrane protein 237 as a novel interactor with the intestinal riboflavin transporter-3 (RFVT-3): role in functionality and cell biology.
Abstract The apically localized riboflavin (RF) transporter-3 (RFVT-3) is involved in intestinal absorption of vitamin B2. Previous studies have characterized different physiological/biological aspects of the RFVT-3, but there is a lack of knowledge regarding possible existence of interacting partner(s) and consequence of interaction (s) on its function/cell biology. To address the latter, we performed yeast two-hybrid (Y2H) screening of a human colonic cDNA library and have identified transmembrane protein 237 (TMEM237) as a putative interactor with the human (h)RFVT-3; the interaction was further confirmed via "...
Source: Am J Physiol Cell Ph... - March 19, 2019 Category: Cytology Authors: Sabui S, Subramanian VS, Pham Q, Said HM Tags: Am J Physiol Cell Physiol Source Type: research

Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1.
Abstract Defective tight junction (TJ) barrier is a key pathogenic factor for Inflammatory Bowel Disease. Previously, we have shown that autophagy, a cell survival mechanism, enhances intestinal epithelial TJ barrier function. Autophagy related protein ATG6/beclin 1, a key protein in the autophagy pathway, also plays a role in the endocytic pathway. The constitutive role of beclin 1 in intestinal TJ barrier is not known. In Caco-2 cells, beclin 1 was found to be co-immunoprecipitated with the TJ protein occludin and co-localized with occludin on the membrane. Treatment of Caco-2 cells with beclin 1 peptide (Tat-be...
Source: Am J Physiol Cell Ph... - March 19, 2019 Category: Cytology Authors: Wong M, Ganapathy AS, Suchanec E, Laidler L, Ma T, Nighot PK Tags: Am J Physiol Cell Physiol Source Type: research

Upregulated MicroRNA-141 Suppresses Epithelial-Mesenchymal Transition and Lymph Node Metastasis via HOXC6-Dependent TGF- β Signaling Pathway in Laryngeal Cancer.
This study investigated the roles of microRNA-141 (miR-141), Homeobox C6 (HOXC6), and the transforming growth factor-β (TGF-β) signaling pathway in epithelial-mesenchymal transition (EMT) and lymph node metastasis in laryngeal cancer. Initially, differentially expressed genes related to laryngeal cancer were retrieved, from which HOXC6 was identified. Next, HOXC6 was determined to be a target gene of miR-141. The regulatory relationship of miR-141, HOXC6 and the TGF-β signaling pathway were then explored accordingly. To further investigate the associated underlying mechanism, Hep-2 cells were transfected with miR-141 mi...
Source: Am J Physiol Cell Ph... - April 23, 2019 Category: Cytology Authors: Sun DZ, Wang XH, Fu YG, Yang PZ, Lv HQ, Yin YW, Zhang XY Tags: Am J Physiol Cell Physiol Source Type: research

Zinc deficiency up-regulates ET-1 mediated secretion and endothelial cell migration through nuclear HIF-1 translocation.
CONCLUSION: Altogether, the results suggest that zinc deficiency up-regulates ET-1 signaling through HIF-1 activation and stimulates endothelial cell migration suggesting an important role of zinc in the vascular consequences of IH and OSA mediated by the HIF-1-ET- signaling. PMID: 31116583 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - May 21, 2019 Category: Cytology Authors: Morand J, Briancon-Marjollet A, Lemarié E, Gonthier B, Arnaud J, Korichneva I, Godin-Ribuot D Tags: Am J Physiol Cell Physiol Source Type: research

The N6-Methyladenosine (m6A)-Forming Enzyme METTL3 Facilitates M1 Macrophage Polarization through the Methylation of STAT1 mRNA.
In conclusion, METTL3 drives M1 macrophage polarization by directly methylating STAT1 mRNA, potentially serving as an anti-inflammatory target. PMID: 31365297 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - July 30, 2019 Category: Cytology Authors: Liu Y, Liu Z, Tang H, Shen Y, Gong Z, Xie N, Zhang X, Wang W, Kong W, Zhou Y, Fu Y Tags: Am J Physiol Cell Physiol Source Type: research

SMCHD1 terminates the first embryonic genome activation event in mouse two-cell embryos and contributes to a transcriptionally repressive state.
Abstract Embryonic genome activation (EGA) in mammals begins with transient expression of a large group of genes (EGA1). Importantly, entry into and exit from the 2C/EGA state is essential for viability. Duxfamily member genes play an integral role in EGA1 by activating other EGA marker genes such as Zscan4family members. We previously reported that Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (Smchd1) is expressed at the mRNA and protein levels in mouse oocytes and early embryos, and that elimination of Smchd1expression inhibits inner cell mass formation, blastocyst formation a...
Source: Am J Physiol Cell Ph... - July 30, 2019 Category: Cytology Authors: Ruebel ML, Vincent KA, Schall PZ, Wang K, Latham KE Tags: Am J Physiol Cell Physiol Source Type: research

Novel Interaction of Antioxidant-1 with TRAF4: Role in Inflammatory Responses in Endothelial Cells.
We reported that in proinflammatory cytokine TNFa-stimulated endothelial cells (ECs), cytosolic Cu chaperone Atox1 functions as a Cu-dependent transcription factor for NOX organizer p47phox, thereby increasing ROS-dependent inflammatory gene expression. However, the role and mechanism of Atox1 nuclear translocation in inflamed ECs remain unclear. Using enface staining and nuclear fractionation, here we show that Atox1 was localized in the nucleus in inflamed aortas from ApoE-/- mice with Angiotensin II infusion on a high fat diet, while it was found in cytosol in those from control mice. In cultured human ECs, TNFα stimul...
Source: Am J Physiol Cell Ph... - September 24, 2019 Category: Cytology Authors: Das A, Sudhahar V, Ushio-Fukai M, Fukai T Tags: Am J Physiol Cell Physiol Source Type: research

Down-regulation of ATAD2 restrains cancer stem cell properties in esophageal squamous cell carcinoma via blockade of the Hedgehog signaling pathway.
Abstract Esophageal squamous cell carcinoma (ESCC) ranks among the five most common cancers in China, and has a five-year survival rate of less than 15%. The transcription factor ATAD2 has potential as a therapeutic target in various tumors, and microarray-based gene expression profiling reveals dysregulation of ATAD2 specifically in ESCC. Here we investigated whether ATAD2 could mediate a regulation of cancer stem cell (CSC) biological functions in ESCC. Immunohistochemical staining, reverse transcription quantitative polymerase chain reaction, and Western blot assays all revealed up-regulation of ATAD2 in ESCC t...
Source: Am J Physiol Cell Ph... - November 19, 2019 Category: Cytology Authors: Li N, Yu Y, Wang B Tags: Am J Physiol Cell Physiol Source Type: research