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Source: Am J Physiol Cell Ph...

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Total 155 results found since Jan 2013.

Specific Inhibition of HDAC4 in Cardiac Progenitor Cells Enhances Myocardial Repairs.
Abstract We have recently shown that in vivo inhibition of histone deacetylase (HDAC) stimulates endogenous myocardial regeneration in infarcted hearts. Furthermore, our observation demonstrates that HDAC inhibition promotes cardiogenesis, which is associated with HDAC4 reduction. However, it remains unknown as to whether specific inhibition of HDAC4 modulates cardiac stem cells (CSCs) to facilitate myocardial repair and to preserve cardiac performance. c-kit(+)CSCs were isolated from adult mouse hearts and were transfected with HDAC4siRNA to knockdown HDAC4 of c-kit(+)CSCs. The transfection of HDAC4siRNA caused a...
Source: Am J Physiol Cell Ph... - June 18, 2014 Category: Cytology Authors: Zhang LX, DeNicola M, Qin X, Du J, Ma J, Zhao TY, Zhuang S, Liu PY, Wei L, Qin G, Tang Y, Zhao TC Tags: Am J Physiol Cell Physiol Source Type: research

Kcnj10 is a major type of K+ channel in mouse corneal epithelial cells and plays a role in initiating EGFR signaling.
Abstract We used primary mouse corneal epithelial cells (pMCE) to examine the role of Kcnj10 in determining membrane K(+) conductance and cell membrane potential, and in regulating EGF/TGFA release. Western blot, immunostaining and RT-PCR detected the expression of Kcnj10 in mouse cornea. The single channel recording identified the 20 pS inwardly rectifying K(+) channels in pMCE of WT mice but these channels were absent in Kcnj10(-/-). Moreover, the whole cell recording demonstrates that deletion of Kcnj10 largely abolished the inward K(+) currents and depolarized the cell membrane K(+) reversal potential (an inde...
Source: Am J Physiol Cell Ph... - August 6, 2014 Category: Cytology Authors: Wang L, Zhang C, Su X, Lin D Tags: Am J Physiol Cell Physiol Source Type: research

5-Aminolevulinic Acid Combined with Sodium Ferrous Citrate Ameliorates H2O2-induced Cardiomyocyte Hypertrophy via Activating the MAPK/Nrf2/HO-1 Pathway.
In conclusion, our data suggest that 5-ALA and SFC protect HL-1 cells from H2O2-induced cardiac hypertrophy via activating MAPK/Nrf2/HO-1 signaling pathway. PMID: 25652453 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - February 4, 2015 Category: Cytology Authors: Zhao M, Guo H, Chen J, Wang J, Huang H, Zheng S, Hei M, Li J, Huang S, Li J, Ma XT, Chen Y, Zhao L, Zhuang J, Zhu P Tags: Am J Physiol Cell Physiol Source Type: research

Chronic and selective inhibition of basolateral membrane Na-K-ATPase uniquely regulates brush border membrane Na absorption in intestinal epithelial cells.
Conclusions:Chronic and direct silencing of basolateral Na-K-ATPase uniquely regulates BBM Na absorptive pathways in intestinal epithelial cells. Specifically, while BBM NHE3 is unaffected, SGLT1 is stimulated secondary to enhanced affinity of the co-transporter. PMID: 25652450 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - February 4, 2015 Category: Cytology Authors: Manoharan P, Gayam S, Arthur S, Palaniappan B, Singh S, Dick G, Sundaram U Tags: Am J Physiol Cell Physiol Source Type: research

Caveolin-1 is a checkpoint regulator in hypoxia-induced astrocyte apoptosis via Ras/Raf/ERK pathway.
Abstract Astrocytes, the most numerous cells in the human brain, play a central role in the metabolic homeostasis following hypoxic injury. Caveolin-1 (Cav-1), a transmembrane scaffolding protein, has been shown to converge pro-survival signaling in the central nerve system. The present study aimed to investigate the role of Cav-1 in the hypoxia-induced astrocyte injury. We also examined how Cav-1 alleviates apoptotic astrocyte death. To this end, primary astrocytes were exposed to oxygen-glucose deprivation (OGD) for 6 h and a subsequent 24 h reoxygenation to mimic hypoxic injury. OGD significantly reduced Cav-1 ...
Source: Am J Physiol Cell Ph... - March 22, 2016 Category: Cytology Authors: Xu L, Wang L, Wen Z, Wu L, Jiang Y, Yang L, Xiao L, Xie Y, Ma M, Zhu W, Ye R, Liu X Tags: Am J Physiol Cell Physiol Source Type: research

Canstatin stimulates migration of rat cardiac fibroblasts via secretion of matrix metalloproteinase-2.
In conclusion, this study revealed a novel function of canstatin for inducing cell migration of adult rat cardiac fibroblasts at least in part by ERK phosphorylation through MMP-2 secretion possibly via actin cytoskeletal change. PMID: 28003226 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - December 20, 2016 Category: Cytology Authors: Okada M, Murata N, Yamawaki H Tags: Am J Physiol Cell Physiol Source Type: research

Integration of TRPC6 and NADPH Oxidase Activation in Lysophosphatidylcholine-induced TRPC5 Externalization.
Abstract Lipid oxidation products, including lysophosphatidylcholine (lysoPC), activate TRPC6, and the subsequent increase in intracellular Ca(2+) leads to TRPC5 activation. The goal of this study is to elucidate the steps in the pathway between TRPC6 activation and TRPC5 externalization. Following TRPC6 activation by lysoPC, extracellular regulated kinase (ERK) is phosphorylated. This leads to phosphorylation of p47(phox) and subsequent NADPH oxidase activation with increased production of reactive oxygen species. ERK activation requires TRPC6 opening and influx of Ca(2+) as evidenced by the failure of lysoPC to ...
Source: Am J Physiol Cell Ph... - August 23, 2017 Category: Cytology Authors: Chaudhuri P, Rosenbaum MA, Birnbaumer L, Graham LM Tags: Am J Physiol Cell Physiol Source Type: research

NF- κB-Dependent Upregulation of (Pro)Renin Receptor Mediates High NaCl-Induced Apoptosis in Mouse Inner Medullary Collecting Duct Cells.
In conclusion, PRR expression is induced by high NaCl through NF-κB and contributes to cell apoptosis under this circumstance. PMID: 29021196 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - October 11, 2017 Category: Cytology Authors: Su J, Liu X, Xu C, Lu X, Wang F, Fang H, Lu A, Qiu Q, Li C, Yang T Tags: Am J Physiol Cell Physiol Source Type: research

The role of microRNA-1 targeting MAPK3 in myocardial ischemia/reperfusion injury in rats undergoing sevoflurane preconditioning via the PI3K/Akt pathway.
Abstract Recent studies have uncovered the vital roles played by microRNAs (miRNAs) in regulating cardiac injury. Amongst them, the cardiac enriched microRNA-1 (miR-1) has been extensively studied and proven to be detrimental to cardiac myocytes. Hence, the current study aims to explore whether miR-1 affects myocardial ischemia/reperfusion injury (MIRI) in rats undergoing sevoflurane preconditioning and the underlying mechanism. After successful model establishment, rats with MIRI were transfected with mimics or inhibitors of miR-1, or siRNA against MAPK3, and then were injected with sevoflurane. A luciferase repo...
Source: Am J Physiol Cell Ph... - May 9, 2018 Category: Cytology Authors: Hao YL, Fang HC, Zhao HL, Li XL, Luo Y, Wu BQ, Fu MJ, Liu W, Liang JJ, Chen XH Tags: Am J Physiol Cell Physiol Source Type: research

Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via Wnt/ β-catenin signaling pathway.
Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via Wnt/β-catenin signaling pathway. Am J Physiol Cell Physiol. 2018 Aug 01;: Authors: Gao D, Chen HQ, Yang YM Abstract Metastatic cutaneous squamous cell carcinoma (CSCC) is a major cause of death associated with non-melanoma skin cancer. The involvement of homeobox B7 (HOXB7) in cancers has been reported. Thus, the current study intends to explore the effect of HOXB7 on CSCC and its relationship with the Wnt/β-catenin signaling pathway. Initially, microarray-based gene expres...
Source: Am J Physiol Cell Ph... - August 1, 2018 Category: Cytology Authors: Gao D, Chen HQ, Yang YM Tags: Am J Physiol Cell Physiol Source Type: research

Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/ β-catenin signaling pathway.
Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway. Am J Physiol Cell Physiol. 2018 Nov 01;315(5):C675-C686 Authors: Gao D, Chen HQ Abstract Metastatic cutaneous squamous cell carcinoma (CSCC) is a major cause of death associated with nonmelanoma skin cancer. The involvement of homeobox B7 ( HOXB7) in cancers has been reported. Thus, the current study intends to explore the effect of HOXB7 on CSCC and its relationship with the Wnt/β-catenin signaling pathway. Initially, microarray-based g...
Source: Am J Physiol Cell Ph... - November 1, 2018 Category: Cytology Authors: Gao D, Chen HQ Tags: Am J Physiol Cell Physiol Source Type: research

MicroRNA-143 inhibits proliferation and promotes apoptosis of thymocyte by targeting CXCL13 in myasthenia gravis mice models.
Abstract Myasthenia gravis (MG) is an autoimmune neuromuscular disorder, affecting the quality of life of millions of people worldwide. The current study aims to determine the relationship between microRNA-143 (miR-143) and CXCL13, and whether it influences the pathogenesis of myasthenia gravis (MG). Thymus specimens were resected from patients with thymic hyperplasia combined MG, and then infused into normal mouse cavities to establish MG mice models. Immunohistochemistry, RT-qPCR, in situ hybridization detection, and Western blot analysis were employed to identify the expression of miR-143 and CXCL13 in MG and n...
Source: Am J Physiol Cell Ph... - November 7, 2018 Category: Cytology Authors: Wu DM, Wen X, Han XR, Wang S, Wang YJ, Shen M, Fan SH, Zhuang J, Zhang ZF, Shan Q, Li MQ, Hu B, Sun CH, Lu J, Zheng YL Tags: Am J Physiol Cell Physiol Source Type: research

Polymerase delta interacting protein 2 activates the RhoGEF Epithelial cell transforming sequence 2 in vascular smooth muscle cells.
In this study, we aimed to better understand how Poldip2 activates Rho family GTPases and the functions of the involved proteins in vascular smooth muscle cells (VSMCs). RhoA is activated by guanine nucleotide exchange factors (GEFs). Using nucleotide-free RhoA (isolated from bacteria) to pulldown active RhoGEFs, we found that the RhoGEF Epithelial cell transforming sequence 2 (Ect2) is activated by Poldip2. Ect2 is a critical RhoGEF for Poldip2-mediated RhoA activation, because siRNA against Ect2 prevented Poldip2-mediated RhoA activity (measured by rhotekin pulldowns). Surprisingly, we were unable to detect a direct inte...
Source: Am J Physiol Cell Ph... - February 6, 2019 Category: Cytology Authors: Huff LP, Kikuchi DS, Faidley E, Forrester SJ, Tsai MZ, Lassègue B, Griendling KK Tags: Am J Physiol Cell Physiol Source Type: research

Neurogranin is expressed in mammalian skeletal muscle and inhibits calcineurin signaling and myoblast fusion.
Abstract Calcineurin is a Ca2+/calmodulin(CaM)-dependent phosphatase that plays a critical role in promoting the slow fiber phenotype and myoblast fusion in skeletal muscle, thereby making calcineurin an attractive cellular target for enhancing fatigue resistance, muscle metabolism, and muscle repair. Neurogranin (Ng) is a CaM-binding protein thought to be expressed solely in brain and neurons, where it inhibits calcineurin signaling by sequestering CaM, thus lowering its cellular availability. Here, we demonstrate for the first time the expression of Ng protein and mRNA in mammalian skeletal muscle. Both protein ...
Source: Am J Physiol Cell Ph... - August 20, 2019 Category: Cytology Authors: Fajardo VA, Watson CJF, Bott KN, Moradi F, Maddalena LA, Bellissimo CA, Turner KD, Peters SJ, LeBlanc PJ, MacNeil AJ, Stuart JA, Tupling AR Tags: Am J Physiol Cell Physiol Source Type: research

Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.
In conclusion, our results showed that the pathophysiological factors in type 2 diabetes, including AGEs, were able to induce centrosome amplification. It is suggested that secreted Wnt6 binds to FZD4 to activate the canonical Wnt6 signaling pathway which is upstream of ROCK1 and 14-3-3σ, which is the cell signaling pathway underlying the diabetes-associated centrosome amplification. PMID: 31618077 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - October 15, 2019 Category: Cytology Authors: He QJ, Wang P, Liu Q, Wu Q, Li YF, Wang J, Lee SC Tags: Am J Physiol Cell Physiol Source Type: research