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Complement C5b-9 and Cancer: Mechanisms of Cell Damage, Cancer Counteractions, and Approaches for Intervention
In conclusion, osmotic burst of inflated complement-damaged cells may occur, but these bursts are most likely a consequence of metabolic collapse of the cell rather than the cause of cell death. The Complement Cell Death Mediator: A Concerted Action of Toxic Moieties Membrane pores caused by complement were first visualized by electron microscopy on red blood cell membranes as large ring structures (22). Similar lesions were viewed on E. coli cell walls (23). Over the years, ample information on the fine ultrastructure of the MAC that can activate cell death has been gathered (24) and has been recently further examined (...
Source: Frontiers in Immunology - April 9, 2019 Category: Allergy & Immunology Source Type: research

German Cockroach Extract Induces Matrix Metalloproteinase-1 Expression, Leading to Tight Junction Disruption in Human Airway Epithelial Cells.
CONCLUSION: GCE treatment increases MMP1 expression, leading to tight junction disruption, which is transcriptionally regulated and influenced by the ERK/MAPK pathway in airway epithelial cells. These findings may contribute to developing novel therapeutic strategies for airway diseases. PMID: 30450857 [PubMed - in process]
Source: Yonsei Medical Journal - November 21, 2018 Category: Universities & Medical Training Authors: Lee KE, Jee HM, Hong JY, Kim MN, Oh MS, Kim YS, Kim KW, Kim KE, Sohn MH Tags: Yonsei Med J Source Type: research

Identifying a Murine Xenograft Model Relevant to Light-Chain Specific Approaches to Human Ig Light-Chain Diseases
CONCLUSIONS: The ALMC-1, RPMI 8226 and H929 IP models did not meet the criteria we required in order to effectively test specific interventions on circulating LC (Table 1). The NSG JJN3 IP model had a short latency period for LC, β2M and FLUX measurements, and had significant correlations among these measurements. The NSG JJN3 model met our criteria. We are currently evaluating soluble BCMA levels in this model also, These measurements will allow investigators to distinguish the impact of interventions on LC specifically, independent of tumor cells. We have used this model successfully to test an RNAi approach to redu...
Source: Blood - November 21, 2018 Category: Hematology Authors: Kugelmass, A., Zhou, P., Ma, X., Toskic, D., Godara, A., Warner, M., Lee, L. X., Fogaren, T., Varga, C., Comenzo, R. L. Tags: 652. Myeloma: Pathophysiology and Pre-Clinical Studies, excluding Therapy Source Type: research

Silencing of telomere-binding protein adrenocortical dysplasia homolog (ACD) enhances radiosensitivity in glioblastoma cells
This study investigated the expression profile of ACD in human gliomas and its role in radioresistance of glioma cells. The expression of ACD was analyzed in sixty-two different grade of glioma tissues and correlated with prognosis. A radioresistant cell line was generated from U87MG cells. For mechanistic studies, ACD was inhibited by small interfering RNA (siRNA) targeting ACD and the effect on cell radioresistance, telomerase activity, cyclinD1, caspase-3, hTERT, and BIRC1 was evaluated.
Source: Translational Research - July 19, 2018 Category: Research Authors: Francesca Polito, Maria Cucinotta, Rosaria V. Abbritti, Anna Brogna, Stefano Pergolizzi, Chiara Tomasello, Flavio F. Angileri, Rosa Maria Di Giorgio, Alfredo Conti, Domenico La Torre, Antonino German ò, M'hammed Aguennouz Source Type: research

Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies
Conclusions and general significanceThese basic associations are equivalent to those observed in D. melanogaster, implying that they have been evolutionary conserved from at least cockroach to flies, and possibly stem from the last common ancestor of extant insects.
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

GSE77621 Multiplex bisulfite sequencing identifies differentially methylated regions in target genes in embryonic cardiomyocytes following knockdown of DNMT3a or DNMT3b expression
This study aims to identify the differentially methylated regions in target genes in embryonic cardiomyocytes following the knockdown of DNMT3a or DNMT3b expression. Methods: Primary cardiomyocytes were isolated from mouse embryonic day (E) 13.5 ventricles, and cultured for 48 h at 37 °C to reach 70-80% confluency. Cells were transfected with either Qiagen FlexiTube GeneSolution (with 4 siRNAs) DNMT3a siRNA (#GS13435), DNMT3b siRNAs (#GS13436), or AllStars negative control siRNA (#1027281) at 0.012 pmol using lipofectamine RNAiMAX (n=3 per treatment). At 72 h after transfection, cells were released with Accutase (Innovati...
Source: GEO: Gene Expression Omnibus - September 8, 2017 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Mus musculus Source Type: research

GSE77514 RNA sequencing identifies differentially expressed genes in embryonic cardiomyocytes following knockdown of DNMT3a or DNMT3b expression
This study aims to identify the differentially expressed genes in embryonic cardiomyocytes following the knockdown of DNMT3a or DNMT3b expression. Methods: Primary cardiomyocytes were isolated from mouse embryonic day (E) 13.5 ventricles, and cultured for 48 h at 37 °C to reach 70-80% confluency. Cells were transfected with either Qiagen FlexiTube GeneSolution (with 4 siRNAs) DNMT3a siRNA (#GS13435), DNMT3b siRNAs (#GS13436), or AllStars negative control siRNA (#1027281) at 0.012 pmol using lipofectamine RNAiMAX (n=3 per treatment). At 72 h after transfection, cells were released with Accutase (Innovative Cell Technologie...
Source: GEO: Gene Expression Omnibus - September 8, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

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