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Total 44 results found since Jan 2013.

Adeno-associated virus type 8 vector-mediated expression of siRNA targeting vascular endothelial growth factor efficiently inhibits neovascularization in a murine choroidal neovascularization model.
CONCLUSIONS: These results suggest that siRNA-VEGF can be expressed across the retina and that long-term suppression of CNV is possible through the use of stable AAV2/8-mediated siRNA-VEGF expression. In vivo gene therapy may thus be a feasible approach to the clinical management of CNV in conditions such as age-related macular degeneration. PMID: 24744609 [PubMed - indexed for MEDLINE]
Source: Molecular Vision - November 16, 2014 Category: Molecular Biology Tags: Mol Vis Source Type: research

A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction
Biomaterials. 2022 Apr 28;285:121541. doi: 10.1016/j.biomaterials.2022.121541. Online ahead of print.ABSTRACTObesity is the major risk factor for metabolic diseases such as fatty liver, hyperlipidemia and insulin resistance. Beige fat has been recognized as a therapeutic target considering its great potential to burn energy. Since the evolutionary discovery of RNA interference and its utilization for gene knockdown in mammalian cells, a remarkable progress has been achieved in siRNA-based therapeutics. However, efficient delivery of siRNA into adipose tissues or differentiated adipocytes is challenging due to high lipid co...
Source: Biomaterials - May 9, 2022 Category: Materials Science Authors: Jin Qiu Qianqian Fan Sainan Xu Dongmei Wang Juntong Chen Sainan Wang Tianhui Hu Xinran Ma Yiyun Cheng Lingyan Xu Source Type: research

Cholesterol modification of SDF-1-specific siRNA enables therapeutic targeting of angiogenesis through Akt pathway inhibition.
Abstract Neovascularization during ocular tissue repair can cause severe visual loss in the optical axis and is therefore an issue of considerable concern to ophthalmologists. Here, we introduced a cholesterol-modified siRNA delivery system targeting stromal cell-derived factor 1 (SDF-1) to treat ocular angiogenesis in vivo. SDF-1 expression was analyzed in rat endothelial progenitor cells (EPCs) and bone marrow mesenchymal stem cells (BMSCs) using quantitative PCR (qPCR). Migration ability of BMSC and HUVEC were assessed through transwell assay. The proliferation effect of chol-siSDF1 on HUVEC was measured by col...
Source: Experimental Eye Research - March 17, 2019 Category: Opthalmology Authors: Chen J, Li F, Xu Y, Zhang W, Hu Y, Fu Y, Xu W, Ge S, Fan X, Lu L Tags: Exp Eye Res Source Type: research

Therapeutic potential of topical administration of siRNAs against HIF-1 α for corneal neovascularization
In conclusion, the topical application of siRNA towards HIF-1α seems to be an effective and reliable method to stop neovascularization.PMID:35367249 | DOI:10.1016/j.exer.2022.109036
Source: Cell Research - April 3, 2022 Category: Cytology Authors: Assumpta Peral Jes ús Mateo Carmen O Dom ínguez-Godínez Gonzalo Carracedo Jose Antonio G ómez Almudena Crooke Jes ús Pintor Source Type: research

Oligonucleotides —A Novel Promising Therapeutic Option for IBD
Conclusions In this review, we focused on recent and past approaches to test the therapeutic efficacy of oligonucleotide based therapies in IBD. The combining mechanistic mode of oligonucleotide based therapeutics is a targeted action on specific pro-inflammatory molecules, which are over activated in IBD patients and contribute significantly to disease pathogenesis. The proposed high selectivity of the agents is derived from its mode of action, that aims to specifically block certain inflammatory molecular patterns, without a general systemic effect on other molecular targets. It would be important for each oligonucleot...
Source: Frontiers in Pharmacology - April 23, 2019 Category: Drugs & Pharmacology Source Type: research

Inhibitory effects of S100A4 gene silencing on alkali burn-induced corneal neovascularization: an in vivo study.
CONCLUSIONS: These findings indicate that S100A4 gene silencing can inhibit alkali burn-induced CNV in rabbits. PMID: 28479848 [PubMed - in process]
Source: Molecular Vision - May 10, 2017 Category: Molecular Biology Tags: Mol Vis Source Type: research

Role of Homeodomain-Interacting Protein Kinase 2 in the Pathogenesis of Tissue Fibrosis in Keloid-Derived Keratinocytes
Abstract: Epithelial-mesenchymal transition (EMT) plays a critical role in fibrotic keloid formation, which is characterized by excessive collagen and extracellular matrix synthesis and deposition. Growing evidence suggests that the serine/threonine kinase homeodomain-interacting protein kinase 2 (HIPK2) acts upstream of several major fibrosis signaling pathways; however, the role of HIPK2 in the keloid fibrogenesis remains unknown. In the current study, we investigated the roles of HIPK2 in the pathogenesis of keloids. Primary normal skin and keloid keratinocytes were cultured and pretreated with transforming growth facto...
Source: Annals of Plastic Surgery - November 11, 2017 Category: Cosmetic Surgery Tags: Burn Surgery and Research Source Type: research

Inhibition of Notch Intracellular Domain Suppresses Cell Activation and Fibrotic Factors Production in Hypertrophic Scar Fibroblasts Versus Normal Skin Fibroblasts
Conclusions Conclusively, the study demonstrates a potential role for Notch signaling in HS progression, and targeting this pathway may provide a novel strategy for treatment of HS.
Source: Annals of Plastic Surgery - March 25, 2021 Category: Cosmetic Surgery Tags: Burn Surgery and Research Source Type: research

Sphingomyelin synthase 1 activity is regulated by the BCR-ABL oncogene Research Articles
Sphingomyelin synthase (SMS) produces sphingomyelin while consuming ceramide (a negative regulator of cell proliferation) and forming diacylglycerol (DAG) (a mitogenic factor). Therefore, enhanced SMS activity could favor cell proliferation. To examine if dysregulated SMS contributes to leukemogenesis, we measured SMS activity in several leukemic cell lines and found that it is highly elevated in K562 chronic myelogenous leukemia (CML) cells. The increased SMS in K562 cells was caused by the presence of Bcr-abl, a hallmark of CML; stable expression of Bcr-abl elevated SMS activity in HL-60 cells while inhibition of the tyr...
Source: The Journal of Lipid Research - February 12, 2013 Category: Lipidology Authors: Burns, T. A., Subathra, M., Signorelli, P., Choi, Y., Yang, X., Wang, Y., Villani, M., Bhalla, K., Zhou, D., Luberto, C. Tags: Research Articles Source Type: research

Reliability of the Mouse Model of Choroidal Neovascularization Induced by Laser Photocoagulation Retina
Conclusions. We present our recommended methods for conducting experiments with the mouse laser-induced CNV model to enhance reproducibility and minimize investigator bias.
Source: Investigative Ophthalmology - October 16, 2014 Category: Opthalmology Authors: Poor, S. H., Qiu, Y., Fassbender, E. S., Shen, S., Woolfenden, A., Delpero, A., Kim, Y., Buchanan, N., Gebuhr, T. C., Hanks, S. M., Meredith, E. L., Jaffee, B. D., Dryja, T. P. Tags: Retina Source Type: research

Nestin-Mediated Cytoskeleton Remodeling in Endothelial Cells: a Novel Mechanistic Insight into VEGF-Induced Cell Migration in Angiogenesis.
Abstract Although nestin is highly expressed in poorly differentiated or newly formed proliferating ECs (endothelial cells), the role of this protein in angiogenesis remains unknown. In addition, the cytoskeleton and associated cytoskeleton-binding proteins mediate the migration of vascular endothelial cells. Thus, the aim of the present study was to determine whether VEGF regulates the cytoskeleton or other associated proteins to promote the migration of VECs.Nestin co-expression with CD31 during angiogenesis in alkali-burned rats corneas was examined through immunohistochemical analysis. Western blot analyses re...
Source: American Journal of Physiology. Cell Physiology - December 10, 2014 Category: Cytology Authors: Liang ZW, Wang Z, Chen H, Li C, Zhou T, Yang Z, Yang X, Yang Y, Gao G, Cai W Tags: Am J Physiol Cell Physiol Source Type: research

Nestin-mediated cytoskeletal remodeling in endothelial cells: novel mechanistic insight into VEGF-induced cell migration in angiogenesis
Nestin is highly expressed in poorly differentiated and newly formed proliferating endothelial cells (ECs); however, the role of this protein in angiogenesis remains unknown. Additionally, the cytoskeleton and associated cytoskeleton-binding proteins mediate the migration of vascular ECs. Therefore, the aim of the present study was to determine whether VEGF regulates the cytoskeleton, as well as other associated proteins, to promote the migration of vascular ECs. The coexpression of nestin and CD31 during angiogenesis in alkali-burned rat corneas was examined via immunohistochemical analysis. Western blot analyses revealed...
Source: AJP: Cell Physiology - March 1, 2015 Category: Cytology Authors: Liang, Z.-w., Wang, Z., Chen, H., Li, C., Zhou, T., Yang, Z., Yang, X., Yang, Y., Gao, G., Cai, W. Tags: CALL FOR PAPERS Source Type: research