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The inhibition of miR-17-5p promotes cortical neuron neurite growth via STAT3/GAP-43 pathway.
Abstract Spinal cord injury (SCI) is a devastating disease associated with locomotor function impair. The limited regenerative capability of the neural axon is one of the major factors that hinders the recovery of SCI. To enhance the regenerative ability of neuron is a promising strategy that repairs SCI. We previously proved miR-17-5p could target Signal Transducer and Activator of Transcription 3 (STAT3) in primary sensory neuron. We speculated that miR-17-5p was the miRNA that targets STAT3. The Dual-luciferase reporter assay indicated miR-17-5p could bind the 3'UTR of STAT3 mRNA. The RT-qPCR and Western blot a...
Source: Molecular Biology Reports - February 23, 2020 Category: Molecular Biology Authors: Zhang L, Wang Z, Li B, Xia Z, Wang X, Xiu Y, Zhang Z, Chen C, Song H, Li W, Yu M, Zhang M, Wang K, Guo X, Ren L, Wang T Tags: Mol Biol Rep Source Type: research

Lentiviral ‑mediated inducible silencing of TLR4 attenuates neuropathic pain in a rat model of chronic constriction injury.
In conclusion, TLR4 may serve a significant role in neuropathy and the results of the present study provide an inducible lentivirus‑mediated siRNA against TLR4 that may serve as a potential novel strategy to be applied in gene therapy for NP in the future. PMID: 30365084 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - October 27, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Epidermal growth factor attenuates blood-spinal cord barrier disruption via PI3K/Akt/Rac1 pathway after acute spinal cord injury.
In this study, we demonstrate that EGF administration inhibits the disruption of BSCB permeability and improves the locomotor activity in SCI model rats. Inhibition of the PI3K/Akt pathways by a specific inhibitor, LY294002, suppresses EGF-induced Rac1 activation as well as tight junction (TJ) and adherens junction (AJ) expression. Furthermore, the protective effect of EGF on BSCB is related to the activation of Rac1 both in vivo and in vitro. Blockade of Rac1 activation with Rac1 siRNA downregulates EGF-induced TJ and AJ proteins expression in endothelial cells. Taken together, our results indicate that EGF treatment pres...
Source: J Cell Mol Med - January 15, 2016 Category: Molecular Biology Authors: Zheng B, Ye L, Zhou Y, Zhu S, Wang Q, Shi H, Chen D, Wei X, Wang Z, Li X, Xiao J, Xu H, Zhang H Tags: J Cell Mol Med Source Type: research

Epidermal growth factor attenuates blood‐spinal cord barrier disruption via PI3K/Akt/Rac1 pathway after acute spinal cord injury
In this study, we demonstrate that EGF administration inhibits the disruption of BSCB permeability and improves the locomotor activity in SCI model rats. Inhibition of the PI3K/Akt pathways by a specific inhibitor, LY294002, suppresses EGF‐induced Rac1 activation as well as tight junction (TJ) and adherens junction (AJ) expression. Furthermore, the protective effect of EGF on BSCB is related to the activation of Rac1 both in vivo and in vitro. Blockade of Rac1 activation with Rac1 siRNA downregulates EGF‐induced TJ and AJ proteins expression in endothelial cells. Taken together, our results indicate that EGF treatment ...
Source: Journal of Cellular and Molecular Medicine - January 1, 2016 Category: Molecular Biology Authors: Binbin Zheng, Libing Ye, Yulong Zhou, Sipin Zhu, Qingqing Wang, Hongxue Shi, Daqing Chen, Xiaojie Wei, Zhouguang Wang, Xiaokun Li, Jian Xiao, Huazi Xu, Hongyu Zhang Tags: Original Article Source Type: research

Silencing ephrinB3 improves functional recovery following spinal cord injury.
Abstract EphrinB3 may restrict axonal regeneration and recovery following traumatic injury of the adult mammalian central nervous system (CNS). Therefore, inhibition of ephrinB3 expression may enhance the ability of the nervous system to regenerate following damage. In the present study, lentiviral expressing vectors, pGCSIL-GFP, expressing an active small interfering RNA (siRNA) targeting the EphB3 sequence, were used to determine the effect of inhibiting EphB3 on nerve functional recovery and regeneration. Basso-Beattie-Bresnahan (BBB) locomotor scores and growth-associated protein, 43 kDa (GAP43) expression le...
Source: Molecular Medicine - March 6, 2014 Category: Molecular Biology Authors: Qu Y, Zhao J, Wang Y, Gao Z Tags: Mol Med Rep Source Type: research