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Specialty: Biochemistry
Cancer: Adenocarcinoma

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Total 32 results found since Jan 2013.

LncRNA AP000695.2 promotes glycolysis of lung adenocarcinoma via the miR-335-3p/TEAD1 axis
This study aims to verify and explore the mechanism of AP000695.2 in glycolysis of LUAD. Overexpression plasmid and siRNA are used to construct cell models of upregulation and downregulation of AP000695.2, respectively. AP000695.2 is highly expressed in lung cancer cell lines as revealed by qPCR. Western blot analysis, FDG uptake, lactate production assay and ECAR determination results show that high expression of AP000695.2 facilitates glycolysis of LUAD cells. CCK-8, EdU staining, Transwell and wound healing assays show that high expression of AP000695.2 promotes cell growth and migration of LUAD. The relationship betwee...
Source: Acta Biochimica et Biophysica Sinica - September 19, 2023 Category: Biochemistry Authors: Shuoyan Xu Zhiming Cheng Bulin Du Yao Diao Yaming Li Xuena Li Source Type: research

The Expression and Effect of ABCE1 in Gastric Adenocarcinoma
Appl Biochem Biotechnol. 2022 Jun 23. doi: 10.1007/s12010-022-03986-6. Online ahead of print.ABSTRACTATP-binding cassette E1 (ABCE1) is mainly related to the regulation of viral infection, cell multiplication, and anti-apoptosis. Previous reports confirmed the central role in the regulation of ABCE1 in liver and breast cancer; however, its potential role in gastric adenocarcinoma remains unclear. In our study, siRNA and plasmid were transfected to construct gastric cancer cell lines with low and overexpression of ABCE1, and Western blot, RT-qPCR, and immunohistochemical staining were used to detect ABCE1 expression levels ...
Source: Applied Biochemistry and Biotechnology - June 23, 2022 Category: Biochemistry Authors: Lei Xing Cuihong Sun Lei Han Zhensheng Zhu Source Type: research

Hypoxic induction of apoptosis occurs through HIF-1 α and accompanies mammalian sterile 20-like kinase 2 cleavage in human endometrial adenocarcinoma Ishikawa cells
Biochem Biophys Res Commun. 2022 Mar 4;604:104-108. doi: 10.1016/j.bbrc.2022.03.016. Online ahead of print.ABSTRACTThe incidence of endometrial cancer is increasing worldwide. One of the main causes of this cancer is a hormone imbalance; progesterone derivatives have been used for treatment. However, reports have shown that hypoxia plays important and possibly beneficial roles in endometrial function. Here, we show the effect of hypoxia on the proliferation of human endometrial adenocarcinoma Ishikawa cells. Hypoxia induced caspase-dependent apoptosis in Ishikawa cells. Overexpression and siRNA-mediated knockdown of hypoxi...
Source: Biochemical and Biophysical Research communications - March 18, 2022 Category: Biochemistry Authors: Jeonggeun Lee Namkyu Lee Hee Dong Han YoungJoo Lee Source Type: research

Long non-coding RNA CCAT1 is overexpressed in endometrial cancer and regulates growth and transcriptome of endometrial adenocarcinoma cells.
CONCLUSIONS: Given that the lncRNA CCAT1 was found to be overexpressed in endometrial cancer, affected the growth of HEC-1B cells and the expression of growth regulatory genes, our data suggest CCAT1 to exert oncogenic functions in endometrial cancer and encourage further studies to examine to what extent this lncRNA might be a potential therapy target in this cancer entity. PMID: 32173521 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - March 11, 2020 Category: Biochemistry Authors: Treeck O, Skrzypczak M, Schüler-Toprak S, Weber F, Ortmann O Tags: Int J Biochem Cell Biol Source Type: research

MALAT1 affects hypoxia-induced vascular endothelial cell injury and autophagy by regulating miR-19b-3p/HIF-1 α axis.
MALAT1 affects hypoxia-induced vascular endothelial cell injury and autophagy by regulating miR-19b-3p/HIF-1α axis. Mol Cell Biochem. 2020 Jan 13;: Authors: Liu H, Shi C, Deng Y Abstract Cardiovascular disease has become the leading cause of death in the world. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) plays an important role in cardiovascular disease, such as stroke. However, the role of MALAT1 in hypoxia (HYP)-induced vascular endothelial cells (VECs) remains unclear. In the present study, HYP-treated human umbilical vein endothelial cells (HUVECs) were utilized to simulate HY...
Source: Molecular and Cellular Biochemistry - January 12, 2020 Category: Biochemistry Authors: Liu H, Shi C, Deng Y Tags: Mol Cell Biochem Source Type: research

MiR-122-5p suppresses the proliferation, migration, and invasion of gastric cancer cells by targeting LYN.
In this study, we found that miR-122-5p was low-expressed in GC tissues and cell lines by using qRT-PCR. Overexpression of miR-122-5p inhibited the proliferation, migration, and invasion of GC cells by using CCK-8 and transwell assays. On the contrary, downregulation of miR-122-5p promoted the proliferation, migration, and invasion of GC cells. In addition, we found that the expression of LYN, an Src family tyrosine kinase, was inversely correlated with miR-122-5p expression in GC tissues by using western blot analysis, immunohistochemistry, and qRT-PCR assays. Meanwhile, luciferase assay results indicated that LYN is a di...
Source: Acta Biochimica et Biophysica Sinica - December 10, 2019 Category: Biochemistry Authors: Meng L, Chen Z, Jiang Z, Huang T, Hu J, Luo P, Zhang H, Huang M, Huang L, Chen Y, Lu M, Xu AM, Ying S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Guanylate binding protein 1 (GBP-1) promotes cell motility and invasiveness of lung adenocarcinoma.
In conclusion, GBP-1 may enhance lung adenocarcinoma invasiveness by promoting cell motility, and control of GBP-1 expression has the potential to contribute to the development of new therapeutic strategies for lung adenocarcinoma. PMID: 31421831 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - August 13, 2019 Category: Biochemistry Authors: Yamakita I, Mimae T, Tsutani Y, Miyata Y, Ito A, Okada M Tags: Biochem Biophys Res Commun Source Type: research

YAP mediates the positive regulation of hnRNPK on the lung adenocarcinoma H1299 cell growth.
In this study, we observed that the expression pattern of hnRNPK in H1299 lung adenocarcinoma cells varied depending on the cell density in culture. Moreover, hnRNPK stimulated the ability of proliferation and colony formation of H1299 cells, which is important for the multilayered cell growth in culture. We further investigated whether there is an association between hnRNPK and the elements involved in the cell contact inhibition pathway. By using quantitative reverse transcriptase-polymerase chain reaction assay and a YAP activity reporter system, we found that hnRNPK upregulated the mRNA and protein levels and transcrip...
Source: Acta Biochimica et Biophysica Sinica - June 11, 2019 Category: Biochemistry Authors: Xu L, Zhang T, Huang W, Liu X, Lu J, Gao X, Zhang YF, Liu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Combinatory inhibition of TRIM65 and MDM2 in lung cancer cells.
Abstract In addition to the involvement in white matter lesion, tripartite-motif protein family member 65 (TRIM65) has also been implicated in tumorigenesis as a potential oncogene. However, the underlining mechanisms of TRIM65 functions and its clinical implication still remain to be further elucidated. In the present study, we found that TRIM65 binds to the N-terminus of p53 tumor suppressor and thus competes with MDM2 for p53 binding. Intriguingly, analysis of the Cancer Genome Atlas (TCGA) gene alteration database revealed that elevated expression of TRIM65 is mutually exclusive to MDM2 up-regulation in human ...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Chen G, Zhou T, Liu Y, Yu Z Tags: Biochem Biophys Res Commun Source Type: research

Ferroptosis is a lysosomal cell death process.
Abstract Ferroptosis is a form of regulated cell death resulting from iron accumulation and lipid peroxidation. While impaired ferroptosis is tightly linked to human diseases and conditions, the mechanism and regulation of ferroptosis remain largely unknown. Here, we demonstrate that STAT3 is a positive regulator of ferroptosis in human pancreatic ductal adenocarcinoma (PDAC) cell lines. Activation of the MAPK/ERK pathway, but not inhibition of system Xc-, was required for STAT3 activation during erastin-induced ferroptosis. Importantly, pharmacological inhibition and genetic silencing of STAT3 through small molec...
Source: Biochemical and Biophysical Research communications - July 18, 2018 Category: Biochemistry Authors: Gao H, Bai Y, Jia Y, Zhao Y, Kang R, Tang D, Dai E Tags: Biochem Biophys Res Commun Source Type: research

Decrease in paracellular permeability and chemosensitivity to doxorubicin by claudin-1 in spheroid culture models of human lung adenocarcinoma A549 cells.
Abstract Chemotherapy resistance is a major problem in the treatment of cancer, but the underlying mechanisms are not fully understood. We found that the expression levels of claudin-1 (CLDN1) and 3, tight junctional proteins, are upregulated in cisplatin (CDDP)-resistant human lung adenocarcinoma A549 (A549R) cells. A549R cells showed cross-resistance to doxorubicin (DXR). Here, the expression mechanism and function of CLDN1 and 3 were examined. CLDN1 and 3 were mainly localized at tight junctions concomitant with zonula occludens (ZO)-1, a scaffolding protein, in A549 and A549R cells. The phosphorylation levels ...
Source: Biochimica et Biophysica Acta - March 7, 2018 Category: Biochemistry Authors: Akizuki R, Maruhashi R, Eguchi H, Kitabatake K, Tsukimoto M, Furuta T, Matsunaga T, Endo S, Ikari A Tags: Biochim Biophys Acta Source Type: research