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Long non-coding RNA MALAT1 modulates myocardial ischemia-reperfusion injury though the PI3K/Akt/eNOS pathway by sponging miRNA-133a-3p to target IGF1R expression
This study aimed to elucidate the roles and possible mechanism of MALAT1 in myocardial IR injury. IR model was established in rats by ligation of the anterior descending artery in vivo, and H9c2 and HL-1 cells were treated by hypoxia/reoxygenation (HR) to construct the model in vitro. The small interfering RNA (siRNA) for MALAT1 and miR-133a-3p mimics, inhibitor was used to transfect the cells. The expression of MALAT1, miR-133a-3p in MIRI were evaluated using real-time quantitative polymerase chain reaction (qRT-PCR)，immunohistochemistry (IHC) and western blot (WB). Relationships between MALAT1, insulin-like growth fact...
Source: European Journal of Pharmacology - December 30, 2021 Category: Drugs & Pharmacology Authors: Xin-Ming Liu Zhenzhou Zhang Jiuchang Zhong Ning Li Tao Wang Lefeng Wang Qian Zhang Source Type: research

The JAK/STAT Pathway in Skeletal Muscle Pathophysiology
Conclusion and Perspectives The IL-6/JAK/STAT signaling cascade plays a dominant role in skeletal muscle pathophysiology. IL-6 autocrine, paracrine, and endocrine functions assign to its downstream effectors pivotal importance in skeletal muscle-wasting-associated diseases and other multiple system diseases where muscle acts in communication with other organs. Targeting the components of the JAK/STAT pathway recently emerged as a strategic approach for the treatment of inflammatory diseases and human cancer. This review highlights the opposite outcomes on muscle biology caused by the amount of local and systemic release ...
Source: Frontiers in Physiology - April 29, 2019 Category: Physiology Source Type: research

Genetic Regulation of Liver Metabolites and Transcripts Linking to Biochemical-Clinical Parameters
Conclusion In summary, this study is the first to combine metabolomics, transcriptomics, and genome-wide association studies in a porcine model. Our results improve understanding of the genetic regulation of metabolites which link to transcripts and finally biochemical-clinical parameters. Further, high-performance profiling of metabolites as intermediate phenotypes is a potentially powerful approach to uncover how genetic variation affects metabolic and health status. Our results advance knowledge in areas of biomedical and agricultural interest and identify potential correlates of biomarkers, SNPs-metabolites, SNPs-tran...
Source: Frontiers in Genetics - April 16, 2019 Category: Genetics & Stem Cells Source Type: research

Identification of microRNAs involved in gefitinib resistance of non-small-cell lung cancer through the insulin-like growth factor receptor 1 signaling pathway.
Authors: Ma W, Kang Y, Ning L, Tan J, Wang H, Ying Y Abstract Multiple clinical and experimental studies have suggested that epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) may be effective at treating advanced non-small cell lung cancer (NSCLC), however, the molecular basis of primary resistance to EGFR-TKIs in NSCLC remains unclear. In the current study, the insulin-like growth factor 1 receptor (IGF-1R) gene in the gefitinib-resistant human lung adenocarcinoma epithelial cell line A549 (A549/GR) was silenced using small interfering RNA (siRNA) in order to determine the role of microRNA (m...
Source: Experimental and Therapeutic Medicine - September 17, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research

Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure Signal Transduction
Cadmium exposure is known to increase lung cancer risk, but the underlying molecular mechanisms in cadmium-stimulated progression of malignancy are unclear. Here, we examined the effects of prolonged cadmium exposure on the malignant progression of A549 human lung adenocarcinoma cells and the roles of Notch1, hypoxia-inducible factor 1α (HIF-1α), and insulin-like growth factor 1 receptor (IGF-1R)/Akt/extracellular signal-regulated kinase (ERK)/p70 S6 kinase 1 (S6K1) signaling pathways. Exposing A549 cells to 10 or 20 μm cadmium chloride (CdCl2) for 9–15 weeks induced a high proliferative potential, the epithelial-mese...
Source: Journal of Biological Chemistry - May 12, 2017 Category: Chemistry Authors: Kota Fujiki, Hisako Inamura, Takamitsu Miyayama, Masato Matsuoka Tags: Cell Biology Source Type: research

CDX2 inhibits pancreatic adenocarcinoma cell proliferation via promoting tumor suppressor miR-615-5p
In this study, we observed that CDX2 expression was much lower in mouse pancreatic adenocarcinoma tissues and pancreatic cancer cells. A network integrated by ChIPBase platform hinted that miR-615-5p, a most newly discovered tumor suppressor, was probably bound by CDX2 in the promoter region. Chromatin immunoprecipitation (ChIP)-qPCR assay showed that CDX2 exhibited a high capacity of binding to miR-615-5p promoter region compared to the negative control. Real-time PCR and western blotting analyses revealed that CDX2 overexpression caused inflation of miR-615-5p and depression of insulin-like growth factor 2 (IGF2), a dire...
Source: Tumor Biology - August 14, 2015 Category: Cancer & Oncology Source Type: research

Abstract 4440: The functional role of insulin-like growth factor binding protein-2 in esophageal adenocarcinoma chemoresistance
Despite improved understanding of esophageal adenocarcinoma (EAC) progression and advances in endoscopic surveillance and multimodality treatment, patients commonly present with advanced stage disease and demonstrate resistance to therapy, with complete response rates below 40%. Understanding the molecular mechanisms of resistance is crucial for predicting and overcoming this resistance.IGFBP2 is a member of the IGFBP family of proteins that has been shown to modulate both insulin growth factor (IGF) and integrin signaling and is a mediator of cell growth, invasion and resistance in other tumor types. Because these pathway...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Myers, A. L., Lin, L., Nancarrow, D. J., Wang, Z., Ferrer-Torres, D., Beer, D. G., Chang, A. C. Tags: Experimental and Molecular Therapeutics Source Type: research

The involvement of insulin-like growth factor 2 binding protein 3 (IMP3) in pancreatic cancer cell migration, invasion, and adhesion
Conclusions: These results suggest that IMP3 facilitates PDAC progression by enhancing the pro-metastatic behaviour of tumour cells.
Source: BMC Cancer - April 11, 2015 Category: Cancer & Oncology Authors: Clarissa PasiliaoChe-Wei ChangBrent SutherlandShannon ValdezDavid SchaefferDonald YappSylvia Ng Source Type: research

RNA-dependent protein kinase (PKR) depletes nutrients, inducing phosphorylation of AMP-activated kinase in lung cancer.
We examined PKR protein expression in 55 frozen primary lung tumor tissues by Western blotting and analyzed the association between PKR expression and expresson of 139 proteins on tissue samples examined previously by Reverse Phase Protein Array (RPPA) from the same 55 patients. We observed that biomarkers were either positively (phosphorylated AMP-activated kinaseT172 [p-AMPK]) or negatively (insulin receptor substrate 1, meiotic recombination 11, ATR interacting protein, telomerase, checkpoint kinase 1, and cyclin E1) correlated with PKR. We further confirmed that induction of PKR with expression vectors in lung cancer c...
Source: Oncotarget - March 24, 2015 Category: Cancer & Oncology Tags: Oncotarget Source Type: research