Filtered By:
Specialty: Biomedical Science
This page shows you your search results in order of relevance.
Order by Relevance | Date
Total 61743 results found since Jan 2013.
Multiwalled carbon nanotubes co-delivering sorafenib and epidermal growth factor receptor siRNA enhanced tumor-suppressing effect on liver cancer.
CONCLUSIONS: MWNT/Sor/siRNA exhibited a superior antitumor effect in vitro and in vivo.
METHODS: The MWNT/Sor and MWNT/Sor/siRNA were prepared, and then the morphologies of MWNT/Sor/siRNA were analyzed. In vitro Sor release assay, siRNA stability and cellular uptake of MWNT/Sor/siRNA were performed as well. Next, the effects of MWNT, free Sor, free siRNA, MWNT/Sor and MWNT/Sor/siRNA were evaluated by colony-forming assay, and cell apoptosis assay in HepG2 cells. Meanwhile, the level of EGFR and proteins associated with apoptosis was tested. Furthermore, the anti-tumor effects of MWNT/Sor/siRNA on LC xenograft mice wer...
Source: Aging - January 13, 2021 Category: Biomedical Science Authors: Wen Z, Feng Y, Hu Y, Lian L, Huang H, Guo L, Chen S, Yang Q, Zhang M, Wan L, Xu K, Degejirifu D, Yan X Tags: Aging (Albany NY) Source Type: research
Induction of Connective Tissue Growth Factor Expression by Hypoxia in Human Lung Fibroblasts via the MEKK1/MEK1/ERK1/GLI-1/GLI-2 and AP-1 Pathways
by Yi Cheng, Chien-huang Lin, Jing-Yun Chen, Chien-Hua Li, Yu-Tin Liu, Bing-Chang Chen
Several reports have indicated that hypoxia, GLI, and connective tissue growth factor (CTGF) contribute to pulmonary fibrosis in idiopathic pulmonary fibrosis. We investigated the participation of mitogen-activated protein kinase kinase (MEK) kinase 1 (MEKK1)/MEK1/ERK1/GLI-1/2 and activator protei n-1 (AP-1) signaling in hypoxia-induced CTGF expression in human lung fibroblasts. Hypoxia time-dependently increased CTGF expression, which was attenuated by the small interfering RNA (siRNA) of GLI-1 (GLI-1 siRNA) and GLI-2 (GLI-2 siRNA) in ...
Source: PLoS One - August 2, 2016 Category: Biomedical Science Authors: Yi Cheng Source Type: research
Chitosan nanoparticle-delivered siRNA reduces CXCR4 expression and sensitizes breast cancer cells to cisplatin.
Abstract
Chemokine (C-X-C motif) receptor 4 (CXCR4) has been reported as a poor prognotic biomarker in human breast cancers and has been suggested promising as a therapeutic target of breast cancer treatment. The present study aims to investigate the delivery efficiency of siRNA by chitosan into breast cancer cells, and then to examine the regulatory role by chitosan nanoparticle-delivered siRNA on CXCR4 expression and on the chemosensitivity of breast cancer cells. Our results demonstrated that the siRNA could be capsuled by chitosan into nanoparticles with a diameter of 80-110 nm, with a Zeta potential of 20-50 ...
Source: Bioscience Reports - April 26, 2017 Category: Biomedical Science Authors: Yu S, Chen Y, Li X, Gao Z, Liu G Tags: Biosci Rep Source Type: research
Enhanced delivery of biodegradable mPEG-PLGA-PLL nanoparticles loading Cy3-labelled PDGF-BB siRNA by UTMD to rat retina.
Abstract
We investigated the efficacy and safety of ultrasound (US)-targeted microbubble (MB) destruction (UTMD)-enhanced delivery of monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-L-lysine (mPEG-PLGA-PLL) nanoparticles (NPs) loading Cy3-labelled platelet-derived growth factor BB (PDGF-BB) siRNA to rat retina in vivo. Eighty Wistar rats were divided into five groups (G). The right eyes, respectively, received an intravitreal injection as follows: normal saline (NS) (G1), NPs and NS (G2), NPs and MBs (G3), NPs and NS (G4) and NPs and MBs (G5). In G4 and G5, the eyes were exposed to US for 5 min...
Source: Journal of Biosciences - June 1, 2017 Category: Biomedical Science Authors: DU J, Sun Y, Li FH, DU LF, Duan YR Tags: J Biosci Source Type: research
Molecular Mechanisms and Biological Functions of siRNA.
Authors: Dana H, Chalbatani GM, Mahmoodzadeh H, Karimloo R, Rezaiean O, Moradzadeh A, Mehmandoost N, Moazzen F, Mazraeh A, Marmari V, Ebrahimi M, Rashno MM, Abadi SJ, Gharagouzlo E
Abstract
One of the most important advances in biology has been the discovery that siRNA (small interfering RNA) is able to regulate the expression of genes, by a phenomenon known as RNAi (RNA interference). The discovery of RNAi, first in plants and Caenorhabditis elegans and later in mammalian cells, led to the emergence of a transformative view in biomedical research. siRNA has gained attention as a potential therapeutic reagent due t...
Source: International Journal of Biomedical Science - August 23, 2017 Category: Biomedical Science Tags: Int J Biomed Sci Source Type: research
SiRNA-Mediated RRM2 Gene Silencing Combined with Cisplatin in the Treatment of Epithelial Ovarian Cancer In Vivo: An Experimental Study of Nude Mice.
Discussion: siRNA alone or combined with cisplatin can effectively inhibit the growth of human ovarian cancer in nude mice models of subcutaneous transplantation of tumor cells. RRM2 gene silencing may be a potential treatment regimen for ovarian cancer in future.
PMID: 31673243 [PubMed - in process]
Source: International Journal of Medical Sciences - November 2, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research
CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells
Intracellular therapeutic targets that define tumor immunosuppression in both tumor cells and T cells remain intractable. Here, we have shown that administration of a covalently linked siRNA to an aptamer (apt) that selectively binds cytotoxic T lymphocyte–associated antigen 4 (CTLA4apt) allows gene silencing in exhausted CD8+ T cells and Tregs in tumors as well as CTLA4-expressing malignant T cells. CTLA4 expression was upregulated in CD8+ T cells in the tumor milieu; therefore, CTLA4apt fused to a STAT3-targeting siRNA (CTLA4apt–STAT3 siRNA) resulted in internalization into tumor-associated CD8+ T cells and silencing...
Source: Journal of Clinical Investigation - June 3, 2014 Category: Biomedical Science Authors: Andreas Herrmann, Saul J. Priceman, Maciej Kujawski, Hong Xin, Gregory A. Cherryholmes, Wang Zhang, Chunyan Zhang, Christoph Lahtz, Claudia Kowolik, Steve J. Forman, Marcin Kortylewski, Hua Yu Source Type: research
In vivo siRNA delivery of Keap1 modulates death and survival signaling pathways and attenuates concanavalin-A-induced acute liver injury in mice RESEARCH ARTICLE
In conclusion, our results have revealed a potential therapeutic use of in vivo siRNA technology targeted to Keap1 to combat oxidative stress by modulating Nrf2-mediated antioxidant responses and IGFIR survival signaling during the progression of ALF.
Source: DMM Disease Models and Mechanisms - August 21, 2014 Category: Biomedical Science Authors: Gonzalez-Rodriguez, A., Reibert, B., Amann, T., Constien, R., Rondinone, C. M., Valverde, A. M. Tags: Research Article Source Type: research
siRNA suppression of hTERT using activatable cell-penetrating peptides in hepatoma cells.
Abstract
Activatable cell-penetrating peptides (aCPPs) allow nonviral, low cytotoxic, and selective delivery of compounds into target cells for cancer therapy. In tumor cells, up-regulation of human telomerase reverse transcriptase (hTERT) frequently occurs and is being considered as a target in cancer diagnosis and treatment. siRNA sequence that target hTERT mRNA can silence the gene and reduce hTERT protein expression to reduce cell proliferation and inhibit cell growth. In our study, we tested a matrix metalloproteinase-2 (MPP2) activatable cell-penetrating peptide (CPP) in delivering hTERT siRNA into SMMC-7721...
Source: Bioscience Reports - February 11, 2015 Category: Biomedical Science Authors: Li H, He J, Yi H, Xiang G, Chen K, Fu B, Yang Y, Chen G Tags: Biosci Rep Source Type: research
Cystathionine-γ-lyase gene silencing with siRNA in monocytes/ macrophages attenuates inflammation in cecal ligation and puncture-induced sepsis in the mouse.
Abstract
Hydrogen sulphide is an endogenous inflammatory mediator produced by cystathionine-γ-lyase (CSE) in macrophages. To determine the role of H2S and macrophages in sepsis, we used small interference RNA (siRNA) to target the CSE gene and investigated its effect in a mouse model of sepsis. Cecal ligation puncture (CLP)-induced sepsis is characterized by increased levels of myeloperoxidase (MPO) activity, morphological changes in liver and pro-inflammatory cytokines and chemokines in the liver and lung. SiRNA treatment attenuated inflammation in the liver and lungs of mice following CLP-induced sepsis. Liver ...
Source: Journal of Biosciences - March 1, 2016 Category: Biomedical Science Authors: Badiei A, Chambers ST, Gaddam RR, Bhatia M Tags: J Biosci Source Type: research
PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents —Synthesis, physicochemical characterization and in vitro evaluation
We describe the synthesis of cellulose nanocrystals (CNCs) complexed to siRNA. PEI (600Da) was first covalently attached to CNCs by reductive amination reaction, then CNCs-PEI were loaded with siRNA, resulting in the formation of CNCs-PEI-siRNA particles, strongly stabilized by ionic interactions. Efficient cellular uptake of these particles was monitored by ethidium bromide staining. Not only did CNCs-PEI show no cytotoxicity at the studied concentrations, but they also protected siRNA from degradation and favored its delivery into the cells. This siRNA (siRNA killer) is able to silence the expression of cell cycle genes ...
Source: Carbohydrate Polymers - February 9, 2017 Category: Biomedical Science Source Type: research
PEGylation of 6-amino-6-deoxy-curdlan for efficient in vivo siRNA delivery
Publication date: 5 May 2016 Source:Carbohydrate Polymers, Volume 141 Author(s): Altanzul Altangerel, Jia Cai, Lixia Liu, Yinga Wu, Huricha Baigude, Jingfen Han RNA interference (RNAi) is an evolutionarily conserved gene-silencing phenomenon that shows great promise for developing new therapies. However, the development of small interfering RNA (siRNA)-based therapies need to establish efficient delivery system that silences target genes with siRNA doses that is clinically feasible in humans. Here we report synthesis and in vivo study of a novel PEGylated curdlan-based nanoparticle, designated as 6AC-100PEG, obtain...
Source: Carbohydrate Polymers - January 14, 2016 Category: Biomedical Science Source Type: research
Fine-tuned PEGylation of chitosan to maintain optimal siRNA-nanoplex bioactivity
Publication date: 5 June 2016 Source:Carbohydrate Polymers, Volume 143 Author(s): Andra Guţoaia, Liane Schuster, Simona Margutti, Stefan Laufer, Burkhard Schlosshauer, Rumen Krastev, Dieter Stoll, Hanna Hartmann Polyethylene glycol (PEG) is a widely used modification for drug delivery systems. It reduces undesired interaction with biological components, aggregation of complexes and serves as a hydrophilic linker of ligands for targeted drug delivery. However, PEGylation can also lead to undesired changes in physicochemical characteristics of chitosan/siRNA nanoplexes and hamper gene silencing. To address this co...
Source: Carbohydrate Polymers - February 10, 2016 Category: Biomedical Science Source Type: research
Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.
Abstract
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 μg/mL cyclopamine group was 68.9% lower tha...
Source: Biomedical and Environmental Sciences : BES - July 31, 2016 Category: Biomedical Science Authors: Zhou Q, Wu HF, Wei Y, Zhu WL Tags: Biomed Environ Sci Source Type: research
ESM-1 siRNA Knockdown Decreased Migration and Expression of CXCL3 in Prostate Cancer Cells.
We report here for the first time the ESM-1 targeting in PC-3 cells, which resulted in decreased migration, which may be related, at least in part, to decreased expression of the angiogenic CXCL3 chemokine, whose expression was found to be reduced in ESM-1-siRNA transfected cells. Additional studies are required to ascertain the biological role of ESM-1 in prostate cancer cells and the link with the expression of CXCL3.
PMID: 28533735 [PubMed - in process]
Source: International Journal of Biomedical Science - May 25, 2017 Category: Biomedical Science Tags: Int J Biomed Sci Source Type: research
