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Total 111164 results found since Jan 2013.

A new liposomal nanocarrier for co-delivery of gedunin and p-glycoprotein siRNA to target breast cancer stem cells
Nat Prod Res. 2022 Jan 27:1-4. doi: 10.1080/14786419.2022.2032048. Online ahead of print.ABSTRACTGedunin is a secondary metabolite found in neem tree. Since the first discovery of this compound, its bio-active properties have been continuously evaluated. However, the low hydrophobicity of gedunin decreases its bioavailability and pharmacokinetic profile. In the present investigation, a new liposomal nanocarrier for co-delivery of gedunin and P-glycoprotein (P-gp) siRNA [siRNA coated liposomal gedunin (Lipo-Ged-siRNA)] was developed to improve the anti-proliferative activity of gedunin. Characteristics of prepared Lipo-Ged-...
Source: Natural Product Research - January 27, 2022 Category: Biochemistry Authors: Mohan Singh Rana Meran Keshawa Ediriweera Umapriyatharshini Rajagopalan Desiree Nedra Karunaratne Kamani Hemamala Tennekoon Sameera Ranganath Samarakoon Source Type: research

Synergistic effect of siRNA ‐mediated silencing of TGF‐β R1 and TGF‐β R2 genes on the proliferation and apoptosis of penile urethral epithelial cells in hypospadiac male rats
This study ultimately demonstrated that the silencing of TGF‐β R1 and TGF‐β R2 genes could promote cell proliferation and inhibit apoptosis of penile UECs in hypospadiac male rats. This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - August 4, 2017 Category: Biochemistry Authors: Chao Wang, Yan ‐Yan Qiao Tags: Article Source Type: research

siRNA blocking the RAS signalling pathway and inhibits the growth of oesophageal squamous cell carcinoma in nude mice
The aim of this study was to study RAS‐siRNA blocking RAS pathway and suppressing cell growth in human oesophageal squamous cell carcinoma in nude mice. The methods in this study was to construct RAS‐siRNA expression vector, establish 40 oesophageal squamous cell carcinoma xenograft animal models and divided them into five groups: control group, siRNA control group, RAS‐siRNA group, paclitaxel group and RAS‐siRNA and paclitaxel group. We observed tumour growth in nude mice, studied histology by HE staining, tumour growth inhibition by TUNEL assay and detected the RAS, MAPK and cyclin D1 protein expression by immuno...
Source: Cell Biochemistry and Function - November 28, 2014 Category: Biochemistry Authors: Xinjie Wang, Yuling Zheng, Qingxia Fan, Xudong Zhang, Yonggang Shi Tags: Research Article Source Type: research

Transcutaneous iontophoretic delivery of STAT3 siRNA using layer-by-layer chitosan coated gold nanoparticles to treat melanoma
In conclusion, layer-by-layer chitosan coated AuNP can be developed as a carrier for iontophoretic delivery of STAT3 siRNA to treat melanoma. Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - June 16, 2016 Category: Biochemistry Source Type: research

PEGylated DC-Chol/DOPE cationic liposomes containing KSP siRNA as a systemic siRNA delivery Carrier for ovarian cancer therapy.
Abstract Although siRNA-mediated downregulation technology has been highly successful in suppressing the expression of any disease-related gene, systemic delivery of siRNA for the clinical applications remains challenging, especially in the use of cancer therapy. DC-Chol/DOPE cationic liposomes as one of the most attractive vehicles for gene delivery have been widely exploited for transfection of siRNA into cells, but complexity of systemic delivery has allowed only their direct injection into local targets due to the formation of aggregations with negatively-charged blood components. Herein, we demonstrate the ef...
Source: Biochemical and Biophysical Research communications - July 23, 2018 Category: Biochemistry Authors: Lee J, Ahn HJ Tags: Biochem Biophys Res Commun Source Type: research

A polycation coated liposome as efficient siRNA carrier to overcome multidrug resistance
Publication date: 1 November 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 159 Author(s): Yuqiong Xia, Xiaofei Wang, He Cheng, Mei Fang, Pengbo Ning, Yulu Zhou, Wei Chen, Hongjin Song Multidrug resistance (MDR) is one of the important factors that impede effective chemotherapy against cancer. Codelivery of MDR1 siRNA (silencing ABCB1 gene) and anticancer drug can greatly inhibit tumor proliferation. Here in this work, we synthesized poly(diallyldimethylammonium chloride) (PDADMAC) coated liposome formula as siMDR1 carrier (AL-PDAD-RNA) and applied it to reverse doxorubicin resistance of OVCAR8/ADR cells. The ...
Source: Colloids and Surfaces B: Biointerfaces - August 19, 2017 Category: Biochemistry Source Type: research

5'-(E)-Vinylphosphonate: A Stable Phosphate Mimic Can Improve the RNAi Activity of siRNA-GalNAc Conjugates.
Abstract Small interfering RNA (siRNA)-mediated silencing requires siRNA loading into the RNA-induced silencing complex (RISC). Presence of 5'-phosphate (5'-P) is reported to be critical for efficient RISC loading of the antisense strand (AS) by anchoring it to the mid-domain of the Argonaute2 (Ago2) protein. Phosphorylation of exogenous duplex siRNAs is thought to be accomplished by cytosolic Clp1 kinase. However, although extensive chemical modifications are essential for siRNA-GalNAc conjugate activity, they can significantly impair Clp1 kinase activity. Here, we further elucidated the effect of 5'-P on the act...
Source: Chembiochem - April 27, 2016 Category: Biochemistry Authors: Parmar R, Willoughby JL, Liu J, Foster DJ, Brigham B, Theile CS, Charisse K, Akinc A, Guidry E, Pei Y, Strapps W, Cancilla M, Stanton MG, Rajeev KG, Sepp-Lorenzino L, Manoharan M, Meyers R, Maier MA, Jadhav V Tags: Chembiochem Source Type: research

Chimeric flaviviral RNA-siRNA molecules resist degradation by the exoribonuclease Xrn1 and trigger gene silencing in mammalian cells
Chembiochem. 2021 Aug 25. doi: 10.1002/cbic.202100434. Online ahead of print.ABSTRACTRNA is an emerging platform for drug delivery, but the susceptibility of RNA to nuclease degradation remains a major barrier to its implementation in vivo. Here, we engineered flaviviral Xrn1-resistant RNA (xrRNA) motifs to host small interfering RNA (siRNA) duplexes. The xrRNA-siRNA molecules self-assemble in vitro, resist degradation by the conserved eukaryotic 5' to 3' exoribonuclease Xrn1, and trigger gene silencing in 293T cells. The resistance of the molecules to Xrn1 does not translate to stability in blood serum. Nevertheless, our ...
Source: Chembiochem - August 25, 2021 Category: Biochemistry Authors: Cressida Harvey Sven Klassa Esteban Finol Jonathan Hall Alyssa Hill Source Type: research

Effect of the guide strand 3{'}-end structure on the gene silencing potency of asymmetric siRNA
In this study, we explored the effect of the guide strand 3’-end structure on the gene silencing potency of asiRNA. Interestingly, asiRNAs with a 21 nucleotide (nt) guide strand solely composed of RNA resulted in gene silencing more than 6-fold more efficient compared with the corresponding asiRNA guide strand harboring a deoxythymidine dinucleotide (dTdT) at its 3'-end. We demonstrated that the molecular basis of potency of the asiRNA with a 21 nt guide strand composed solely of RNA was due to the enhanced formation of the RNA- induced silencing complex (RISC) and increased affinity towards human Argonaute2 (hAgo2)...
Source: BJ Gene - May 6, 2014 Category: Biochemistry Authors: S Hong, J Park, S Yun, C Lee, C Shin, D Lee Tags: BJ Biomolecules Source Type: research

Sweet Emotion: The Role of Odor-induced Context in the Search Advantage for Happy Facial Expressions.
Abstract The current study investigated the extent to which the concurrent presentation of pleasant and unpleasant odors could modulate the perceptual saliency of happy facial expressions in an emotional visual search task. Whilst a search advantage for happy faces was found in the no odor and unpleasant odor conditions, it was abolished under the pleasant odor condition. Furthermore, phasic properties of visual search performance revealed the malleable nature of this happiness advantage. Specifically, attention towards happy faces was optimized at the start of the visual search task for participants presented wit...
Source: Chemical Senses - December 23, 2017 Category: Biochemistry Authors: Damjanovic L, Wilkinson H, Lloyd J Tags: Chem Senses Source Type: research

Molecular symmetry-constrained systematic search approach to structure solution of the coiled-coil SRGAP2 F-BARx domain
SRGAP2 (Slit – Robo GTPase-activating protein 2) is a cytoplasmic protein found to be involved in neuronal branching, restriction of neuronal migration and restriction of the length and density of dendritic postsynaptic spines. The extended F-BAR (F-BARx) domain of SRGAP2 generates membrane protrusions when expressed in COS-7 cells, while most F-BARs induce the opposite effect: membrane invaginations. As a first step to understand this discrepancy, the F-BARx domain of SRGAP2 was isolated and crystallized after co-expression with the carboxy domains of the protein. Diffraction data were collected from two significantly n...
Source: Acta Crystallographica Section D - November 28, 2016 Category: Biochemistry Authors: Sporny, M. Guez-Haddad, J. Waterman, D.G. Isupov, M.N. Opatowsky, Y. Tags: exhaustive search SRGAP2 F-BAR coiled coil molecular replacement research papers Source Type: research

siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa.
Abstract To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were measured by the E-test method. The efficacy of siRNAs was determined in a murine model of chronic P. aeruginosa lung infection. MexB-siRNAs inhibited both mRNA expression and the activity of P. aeruginosa in vitro. In vivo, siRNA was effective in reducing the bacterial load in the model of chronic lung infectio...
Source: BMB Reports - November 13, 2013 Category: Biochemistry Authors: Gong FY, Zhang DY, Zhang JG, Wang LL, Zhan WL, Qi JY, Song JX Tags: BMB Rep Source Type: research

New Paradigms on siRNA Local Application.
Abstract Small interfering RNA (siRNA) functions through pairing with specific mRNA sequences and results in mRNA degradation, which is becoming a potential therapeutic approach for many diseases caused by altered gene expression. The delivery is a major problem for siRNA application due to its rapid degradation in blood circulation. Various strategies have been proposed to help cellular uptake of siRNA/short or small hairpin RNA (shRNA) successfully. Here we reviewed the recent published data regarding local applications of siRNA. Compared with systematic delivery methods, local delivery of siRNA/shRNA demonstrat...
Source: BMB Reports - July 31, 2014 Category: Biochemistry Authors: Pan M, Ni J, He H, Gao S, Duan X Tags: BMB Rep Source Type: research

Hypoxia ‐induced microRNA‐26b inhibition contributes to hypoxic pulmonary hypertension via CTGF
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - August 17, 2017 Category: Biochemistry Authors: Sijing Zhou, Li Sun, Chao Cao, Peipei Wu, Min Li, Gengyun Sun, Guanghe Fei, Xing Ding, Ran Wang Tags: Article Source Type: research