A polycation coated liposome as efficient siRNA carrier to overcome multidrug resistance

Publication date: 1 November 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 159 Author(s): Yuqiong Xia, Xiaofei Wang, He Cheng, Mei Fang, Pengbo Ning, Yulu Zhou, Wei Chen, Hongjin Song Multidrug resistance (MDR) is one of the important factors that impede effective chemotherapy against cancer. Codelivery of MDR1 siRNA (silencing ABCB1 gene) and anticancer drug can greatly inhibit tumor proliferation. Here in this work, we synthesized poly(diallyldimethylammonium chloride) (PDADMAC) coated liposome formula as siMDR1 carrier (AL-PDAD-RNA) and applied it to reverse doxorubicin resistance of OVCAR8/ADR cells. The AL-PDAD-RNA can load siRNA effectively and release siRNA under physiological conditions, leading to improved tumor inhibition than free DOX without siRNA treatment. Meanwhile, the gene silencing effect of AL-PDAD-RNA was shown to be comparable to that of commercial transfection agent lipofectamine, but with less toxicity. The main novelty of this work is to offer a new type of siRNA carrier (PDADMAC coated liposome, AL-PDAD), which is simple-structured, highly-effective and non-toxic. Therefore, we anticipate that PDADMAC-coated liposomes would be very promising in the application of other siRNA delivery or even plasmid delivery. Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research