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Total 66000 results found since Jan 2013.
i-Fect Delivers IGF1 siRNA to the Mouse Brain
Intraventricular Injections Used to Study Neuronal Survival During Post Natal DevelopmentNeuromics' i-FectTM Transfection Kit continue to be successfully used to deliver siRNA, shRNA and miRNA to cell cultures and the CNS in vivo (via intrathecal, epidural and intraventricular injection). Genes studied include: ABCA, ASIC, β-arrestin, CAV1.2, IGF1, CX3CR1, DOR, ELOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 ,The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK,SNSR1, hTertTRPV1 NOV, Survivin, TLR4, Troy and TRPV1. Related Publications.It is always an honor for one our products to be referenced in one of the...
Source: siRNA and DsiRNA Transfection Efficiency - April 25, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo siRNA delivery to mouse brain i-Fect Gene Silencing intraventicular injections IGF1 gene expression in vivo siRNA Source Type: news
i-Fect siRNA Transfection Successes
Meeting your Gene Expression Analysis Requirements.I have posted many success stories resulting from use of Neuromics' Neuromics' i-FectTM siRNA Transfection Kit. It has been 3 months since my last posting so here I would like to post some recent highlights. Here I feature customer publication demonstrating:Down regulation of Neuroligin 2-data showed unexpected upregulation and pronociceptive effects of the “inhibitory” NL2 in neuropathic pain, suggesting a functional shift of NL2 from inhibition to excitation that changed the synaptic ratio toward higher excitation.Role of IGFPB5 for survival of neurons in...
Source: siRNA and DsiRNA Transfection Efficiency - July 29, 2013 Category: Neuroscience Tags: Neuroligin 2 Gene berrant Histone Deacetylase2 gene IGFBP5 gene NL2 intraventicular delivery of siRNA ADAC2 i-Fect PSD95 intrathecal delivery of siRNA in vivo siRNA Source Type: news
Silencing Cytokines in-vivo with i-Fect
Knocking Down Cytokine to Study Pain ResponseWe have many unique applications published from researchers using our Transfection Kits in vitro and in vivo. Here researchers simultaneously 3 immune/inflammatory response cytokines in vivo: Byung Moon Choi, Soo Han Lee, Sang Mee An, Do Yang Park, Gwan Woo Lee, and Gyu-Jeong Noh. The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats. Korean J Anesthesiol. 2015 Apr;68(2):159-169. English. Published online March 30, 2015. http://dx.doi.org/10.4097/kjae.2015.68.2.159.Protocol: RNAs w...
Source: siRNA and DsiRNA Transfection Efficiency - April 7, 2015 Category: Neuroscience Tags: allodynia analgesia Gene Expression Analysis Gene Silencing i-Fect IL-1B IL-6 inflammatory pain intrathecal delivery of siRNA TNF-alpha Transfection Kits Source Type: news
Systematic literature search in PubMed : A short introduction.
Abstract
In order to identify current (and relevant) evidence for a specific clinical question within the unmanageable amount of information available, solid skills in performing a systematic literature search are essential. An efficient approach is to search a biomedical database containing relevant literature citations of study reports. In this article, we explain step by step how to perform a systematic literature search via PubMed (MEDLINE) by means of an example research question in the field of ophthalmology. First, we demonstrate how to translate the clinical problem into a well-framed and searchable r...
Source: Der Anaesthesist - October 19, 2018 Category: Anesthesiology Authors: Blümle A, Lagrèze WA, Motschall E Tags: Anaesthesist Source Type: research
Auranofin, an Anti-rheumatic Gold Drug, Aggravates the Radiation-Induced Acute Intestinal Injury in Mice
Conclusion
In this study, we found that a non-toxic dose of auranofin significantly aggravated the severity of the radiation-induced intestinal injury. This suggests that auranofin treatment can be an independent factor that influences the risk of intestinal complications after pelvic or abdominal radiotherapy.
Ethics Statement
All the protocols used in this study were approved by the Institutional Animal Care and Use Committee of the Korean Institute of Radiological and Medical Sciences (IACUC permit number: KIRAMS217-0007).
Author Contributions
H-JL, JS, and Y-BL designed the experiments. EL and JK conducted the exp...
Source: Frontiers in Pharmacology - April 23, 2019 Category: Drugs & Pharmacology Source Type: research
Naringenin Produces Neuroprotection Against LPS-Induced Dopamine Neurotoxicity via the Inhibition of Microglial NLRP3 Inflammasome Activation
Conclusions: This study demonstrated that NAR targeted microglial NLRP3 inflammasome to protect DA neurons against LPS-induced neurotoxicity. These findings suggest NAR might hold a promising therapeutic potential for PD.
Background
Parkinson's disease (PD) is the second most prevalent central nervous system (CNS) degenerative disease. It is characterized by slow and progressive loss of dopamine (DA) neurons in the midbrain substantia nigra (SN) with the accumulation of α-synuclein in Lewy bodies and neuritis (1). Although the etiology of PD remains unclear, amounts of studies have suggested that ne...
Source: Frontiers in Immunology - April 30, 2019 Category: Allergy & Immunology Source Type: research
HDAC2 and Anxiety in Alcoholism
The Impact of HDAC2 Gene Expression on AnxietyOur i-Fect Transfection Kit continues to be a potent tool for testing the impact of altered gene expression on behavior. see: SACHIN MOONAT. The Role of Amygdaloid Chromatin and Synaptic Remodeling in Anxiety and Alcoholism. THESIS Submitted as partial fulfillment of the requirements for the degree of Doctor of Philosophy in Neuroscience in the Graduate College of the University of Illinois at Chicago, 2014.The author hypothesized that increased HDAC2 would have a positive impact on anxiety in alchohol preferring (P) rats. Specifically, HDAC2-induced histone modifications ...
Source: siRNA and DsiRNA Transfection Efficiency - November 24, 2014 Category: Neuroscience Tags: Alcoholism Anxiety BDNF Gene Expression Analysis Gene Silencing HDAC2 gene Learning Source Type: news
Intranasal Delivery of Chitosan–siRNA Nanoparticle Formulation to the Brain
Neurodegeneration is characterized by a progressive loss of neuron structure and function. Most neurodegenerative diseases progress slowly over the time. There is currently no cure available for any neurodegenerative disease, and the existing therapeutic interventions only alleviate the symptoms of the disease. The advances in the drug discovery research have come to a halt with a lack of effective means to deliver drugs at the targeted site. In addition, the route of delivering the drugs is equally important as most invasive techniques lead to postoperative complications. This chapter focuses on a non-invasive, intranasal...
Source: Springer protocols feed by Biotechnology - February 25, 2014 Category: Biotechnology Source Type: news
Role of autophagy in sevoflurane-induced neurotoxicity in neonatal rat hippocampal cells.
CONCLUSION: This study demonstrates that sevoflurane may induce hippocampal neuron autophagy in primary cultured hippocampal neuronal cell and that Beclin-1 and Atg5 are involved in the process of sevoflurane-induced autophagy. Exposure of sevoflurane may not only induce autophagy of hippocampal neurons but also activate the apoptosis of hippocampal neurons. Autophagy may play an important role in sevoflurane-induced neurotoxicity in primary cultured hippocampal neuronal cells.
PMID: 29857124 [PubMed - as supplied by publisher]
Source: Brain Research Bulletin - May 29, 2018 Category: Neurology Authors: Xu L, Shen J, Yu L, Sun J, McQuillan PM, Hu Z, Yan M Tags: Brain Res Bull Source Type: research
Propofol inhibits growth and invasion of pancreatic cancer cells through regulation of the miR-21/Slug signaling pathway.
CONCLUSIONS: Propofol can effectively inhibit invasion and induce apoptosis of PANC-1 cells by regulating miR-21/Slug signals.
PMID: 27829997 [PubMed - in process]
Source: American Journal of Translational Research - November 12, 2016 Category: Research Tags: Am J Transl Res Source Type: research
Antidepressants for the treatment of depression in people with cancer
CONCLUSIONS: Despite the impact of depression on people with cancer, the available studies were few and of low quality. This review found a potential beneficial effect of antidepressants against placebo in depressed participants with cancer. However, the certainty of evidence is very low and, on the basis of these results, it is difficult to draw clear implications for practice. The use of antidepressants in people with cancer should be considered on an individual basis and, considering the lack of head-to-head data, the choice of which drug to prescribe may be based on the data on antidepressant efficacy in the general po...
Source: Cochrane Database of Systematic Reviews - March 31, 2023 Category: General Medicine Authors: Giovanni Vita Beatrice Compri Faith Matcham Corrado Barbui Giovanni Ostuzzi Source Type: research
Strategies to optimize MEDLINE and EMBASE search strategies for anesthesiology systematic reviews. An experimental study.
CONCLUSIONS: In view of these results, we recommend that the search terms used should include both preferred and non-preferred terms (i.e. variant spellings and direct/indirect order of the same term) and associated MeSH and EMTREE terms, in order to develop highly-sensitive search strategies for systematic reviews.
PMID: 29340504 [PubMed - as supplied by publisher]
Source: Sao Paulo Medical Journal - January 15, 2018 Category: General Medicine Authors: Volpato ESN, Betini M, Puga ME, Agarwal A, Cataneo AJM, Oliveira LD, Bazan R, Braz LG, Pereira JEG, Dib RE Tags: Sao Paulo Med J Source Type: research
Efficacy and safety of decontamination for N95 respirator reuse: a systematic literature search and narrative synthesis.
CONCLUSIONS: N95 mask reprocessing using either moist heat or vaporous hydrogen peroxide is recommended to ensure healthcare worker safety.
PMID: 32720256 [PubMed - as supplied by publisher]
Source: Canadian Journal of Anaesthesia - July 26, 2020 Category: Anesthesiology Authors: Steinberg BE, Aoyama K, McVey M, Levin D, Siddiqui A, Munshey F, Goldenberg NM, Faraoni D, Maynes JT Tags: Can J Anaesth Source Type: research
Internet search by the patients undergoing hernia surgery about the disease and surgeon selection
ConclusionsLess than two-thirds of the hernia patients make Internet search about their disease. Higher education level, younger age, patient ’s preference for minimally invasive surgery and living in a city positively affect Internet search rates.
Source: Hernia - January 12, 2022 Category: Sports Medicine Source Type: research
