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Specialty: Biochemistry
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Total 109410 results found since Jan 2013.
Stimuli-Responsive and Multifunctional Nanogels
Chem Biodivers. 2023 Sep 17:e202301009. doi: 10.1002/cbdv.202301009. Online ahead of print.ABSTRACTNanogels represent promising drug delivery systems in the biomedical field, designed to overcome challenges associated with standard treatment approaches. Stimuli-responsive nanogels, often referred to as intelligent materials, have garnered significant attention for their potential to enhance control over properties such as drug release and targeting. Furthermore, researchers have recently explored the application of nanogels in diverse sectors beyond biomedicine including sensing materials, catalysts, or adsorbents for envi...
Source: Chemistry and Biodiversity - September 17, 2023 Category: Biochemistry Authors: Ashwani P V Gopika G Josena Jose Arun Krishna K V Franklin John Jinu George Source Type: research
Interactive effects of Pseudomonas entomophila strain 23S and Clavibacter michiganensis subsp. michiganensis on proteome and anti-Cmm compound production
In this study, the interactions between pure cultures of P. entomophila 23S and Cmm were investigated. First, the population dynamics of each bacterium during the interaction was determined using the selective media. Second, the amount of anti-Cmm compound produced by P. entomophila 23S in the presence of Cmm was quantified using HPLC. Lastly, a label-free shotgun proteomics study of P. entomophila 23S, Cmm, and a co-culture was conducted to understand the effects of the interaction of each bacterium at the proteomic level. Compared with the pure culture grown, the total number of proteins decreased in the interaction for ...
Source: Journal of Proteomics - September 17, 2023 Category: Biochemistry Authors: Yoko Takishita Sowmyalakshmi Subramanian Alfred Souleimanov Donald L Smith Source Type: research
Single-molecule analysis of osmolyte-mediated nanomechanical unfolding behavior of a protein domain
Int J Biol Macromol. 2023 Sep 15:126849. doi: 10.1016/j.ijbiomac.2023.126849. Online ahead of print.ABSTRACTThe small organic molecules, known as osmolytes being ubiquitously present in different cell types, affect protein folding, stability and aggregation. However, it is unknown how the osmolytes affect the nanomechanical unfolding behavior of protein domain. Here, we show the osmolyte-dependent mechanical unfolding properties of protein titin immunoglobulin-27 (I27) domain using an atomic force microscopy (AFM)-based single-molecule force spectroscopy. We found that amines and methylamines improved the mechanical stabil...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Manish Bajaj Mohd Muddassir Bumjoon Choi Priyanka Singh Jong Bum Park Surjeet Singh Manisha Yadav Rajesh Kumar Kilho Eom Deepak Sharma Source Type: research
A novel multifunctional chitosan-gelatin/carboxymethyl cellulose-alginate bilayer hydrogel containing human placenta extract for accelerating full-thickness wound healing
This study presents a multifunctional bi-layer hydrogel incorporating HPE for accelerating full-thickness wound healing through sustained HPE release, inhibition of bacteria invasion, and promotion of cell proliferation. The upper layer of the scaffold, known as the dressing layer, is composed of carboxymethyl cellulose and sodium alginate, serving as a supportive layer for cell proliferation. The under layer, referred to as the regenerative layer, is composed of chitosan and gelatin, providing an extracellular matrix-like, porous, moist, and antibacterial environment for cell growth. The scaffold was optimized to replicat...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Saeed Seifi Amir Shamloo Sayed Navid Tavoosi Aram Almasi-Jaf Hossein Shaygani Mohammad Reza Sayah Source Type: research
Activities of cellulose acetate and microcrystalline cellulose on the thermal and morphomechanical performances of a biobased hybrid composite made polybutylene succinate
Int J Biol Macromol. 2023 Sep 15:126918. doi: 10.1016/j.ijbiomac.2023.126918. Online ahead of print.ABSTRACTMicrocrystalline cellulose (MCC-30 wt%) was extruded with a blend of polybutylene succinate (PBS) and cellulose acetate (CADS=2.5-20 wt%) to produce two grades of binary (PBS/CA, PBS/MCC) and ternary (PBS/CA/MCC) specimens by injection into a mold previously thermostated at 22 °C and 78 °C. The structure-property relationships of neat PBS (n-PBS) and PBS-based blends were investigated by Fourier transform infrared (FTIR) spectroscopy, diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, scanning ele...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Thomas Sango Ahmed Koubaa Mohamed Ragoubi Martin Claude Ngueho Yemele Nathalie Leblanc Source Type: research
Chiral resolution of ( ±)-flurbiprofen using molecularly imprinted hydrazidine-modified cellulose microparticles
Int J Biol Macromol. 2023 Sep 15:126928. doi: 10.1016/j.ijbiomac.2023.126928. Online ahead of print.ABSTRACTFlurbiprofen (FP) is one of the non-steroidal anti-inflammatory drugs (NSAIDs) commonly used to treat arthritic conditions. FP has two enantiomers: S-FP and R-FP. S-FP has potent anti-inflammatory effects, while R-FP has nearly no such effects. Herein, molecularly imprinted microparticles produced from hydrazidine-cellulose (CHD) biopolymer for the preferential uptake of S-FP and chiral resolution of (±)-FP were developed. First, cyanoethylcellulose (CECN) was synthesized, and the -CN units were transformed into hyd...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Huda S AlSalem M Monier May Abdullah Abomuti Rua B Alnoman Hussam Y Alharbi Majed S Aljohani Soha T Al-Goul Eslam B Elkaeed Imen Zghab Amira L Shafik Source Type: research
Catalytic efficiency and thermal stability promotion of the cassava linamarase with multiple mutations for better cyanogenic glycoside degradation
In this study, the catalytic activity and thermal stability of enzymes were screened before investigating the degradation efficiency of cyanogenic glycosides with a cassava linamarase mutant K263P-T53F-S366R-V335C-F339C (CASmut) -controlled technique. The CASmut was obtained with the optimum temperature of 45 °C, which was improved by 10 °C. The specific activity of CASmut was 85.1 ± 4.6 U/mg, which was 2.02 times higher than that of the wild type. Molecular dynamics simulation analysis and flexible docking showed there were more hydrogen bonding interactions at the pocket, and the aliphatic glycoside of the linamarin w...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Yongheng Zhong Ye Li Qi Chen Shengyang Ji Minhao Xu Yuqi Liu Xiaodan Wu Shimin Li Kaimian Li Baiyi Lu Source Type: research
Influence of pH and ionic strength on the physicochemical and structural properties of soybean β-conglycinin subunits in aqueous dispersions
In this study, soybean β-conglycinin and its subunit fractions αα' and β were dispersed in solutions with different pH values (3.7, 7.6, and 9.0) at low (5 mM NaCl) and high (400 mM NaCl) ionic strengths, respectively. The solubility, rheology, particle size, zeta potential, microstructure, secondary structure, and tertiary structure of the different dispersions were analyzed using a range of analytical methods. The β-conglycinin, αα'- and β-subunits aggregated near the isoelectric point (pH 3.7). Increasing the ionic strength led to the assembly of more homogeneous units. An increase in ionic strength at pH 7.6 an...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Qian Ju Jieru Wang Hualu Zhou Dingkui Qin Xiaoyan Hu David Julian McClements Guangzhong Luan Source Type: research
TORC1 mediated regulation of mitochondrial integrity and calcium ion homeostasis by Wat1/mLst8 in S. pombe
Int J Biol Macromol. 2023 Sep 15:126907. doi: 10.1016/j.ijbiomac.2023.126907. Online ahead of print.ABSTRACTThe mTOR complexes play a fundamental role in mitochondrial biogenesis and cellular homeostasis. Wat1, an ortholog of mammalian Lst8 is an important component of TOR complex and is essential for the regulation of downstream signaling. Earlier we reported the role of Wat1 in oxidative stress response. Here, we have shown that the abrogation of wat1 causes respiratory defects and mitochondrial depolarization that leads to a decrease in ATP production. The confocal and electron microscopy in wat1Δ cells revealed the fr...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Simmi Anjum Swati Srivastava Lalita Panigrahi Uzair Ahmad Ansari Arun Kumar Trivedi Shakil Ahmed Source Type: research
Bioderived cellulose fibre-guar gum grafted poly (N, N'-dimethylacrylamide) polymer network for controlled release of metformin hydrochloride
Int J Biol Macromol. 2023 Sep 15:126882. doi: 10.1016/j.ijbiomac.2023.126882. Online ahead of print.ABSTRACTAn interpenetrating polymer network (IPN) of areca cellulose and guar gum grafted with poly (N, N'-dimethylacrylamide) was made by microwave irradiation technique. N, N-methylenebisacrylamide (MBA) was used as the crosslinking agent. The network polymer was characterised using Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Powder X-ray Diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM). The chemical interaction between the drug and the polymer was studied usin...
Source: International Journal of Biological Macromolecules - September 17, 2023 Category: Biochemistry Authors: Smitha Rai Ruchira Raychaudhuri Ritu Kudarha Srinivas Mutalik B Vishalakshi K M Usha Source Type: research
Cloning, tissue specificity and regulation of expression of genes of four key enzymes related to long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis by ambient salinity during embryogenesis in the marine teleost Siganus guttatus
Comp Biochem Physiol B Biochem Mol Biol. 2023 Sep 15:110903. doi: 10.1016/j.cbpb.2023.110903. Online ahead of print.ABSTRACTThe rabbitfish Siganus canaliculatus was the first marine teleost reported to possess long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic ability, and the related molecular mechanisms have been well clarified. Here, we investigated the LC-PUFA biosynthesis capability of the congeneric rabbitfish S. guttatus. First, cDNAs of genes for four key enzymes related to LC-PUFA biosynthesis, namely Δ6Δ5 fatty acyl desaturase (fads2) (1335 bp; 445 aa), Δ4 fads2 (1335 bp; 445 aa), and elongation of v...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 17, 2023 Category: Biochemistry Authors: Cuihong You Shuangshuang Miao Zhiyong Xie Siyuan Lin Shuqi Wang Cuiying Chen Li Lin Yanhua Huang Meng Zhou Yewei Dong Yuanyou Li Ping Zhuang Source Type: research
Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research
RNA interference of NADPH-cytochrome P450 reductase increases the susceptibility of Aphis gossypii Glover to sulfoxaflor
In this study, we cloned and characterized the full-length sequence of A. gossypii CPR (AgCPR). The deduced amino acid sequence of AgCPR contains all conserved domains of CPR, which shows high similarity to other insect CPRs and was clustered into a same branch of aphids according to phylogenetic analysis. The transcript of AgCPR was present in all developmental stages, with the highest expression in the adult stage. Furthermore, the expression of AgCPR could be induced by sulfoxaflor, a commonly used insecticide, in a time- and dose-dependent manner. Further silencing of AgCPR by feeding dsRNA significantly increased the ...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - September 17, 2023 Category: Biochemistry Authors: Qiuling Tang Xuchao Li Yanping He Kangsheng Ma Source Type: research
Functionalized fluorescent organic nanoparticles based AIE enabling effectively targeting cancer cell imaging
We report a fluorescent dye TM by incorporating the tetraphenylethylene (TPE) and cholesterol components into perylene bisimides (PBI) derivative. Fluorescence emission spectrum shows that the dye has stable red emission and aggregation-induced emission (AIE) characteristics. The incorporation of cholesterol components triggers TM to show induced chirality through supramolecular self-assembly. The cRGD-functionalized nanoparticles were prepared by encapsulating fluorescent dyes with amphiphilic polymer matrix. The functionalized fluorescent organic nanoparticles exhibit excellent biocompatibility, large Stokes' shift and g...
Source: Chembiochem - September 17, 2023 Category: Biochemistry Authors: Jing Zhang Jiaqi Liu Chengyan Niu Qiulan Wu Jingjing Tan Ning Jing Ying Wen Source Type: research
HSAFM-MIREBA - Methodology for Inferring REsolution in biological applications
Anal Biochem. 2023 Sep 15:115320. doi: 10.1016/j.ab.2023.115320. Online ahead of print.ABSTRACTDue to a lack of requirement for any direct labelling of the target molecule, high speed atomic force microscopy (HS-AFM) is a potentially powerful procedure for the assessment of biological processes involving macromolecules. When the sample is static the AFM device can be purposefully setup to recover high-resolution information about the feature in question. However, when the feature to be studied moves an appreciable amount during the course of the measurement, the obtained image will be blurred. Encountering such blurred obs...
Source: Analytical Biochemistry - September 17, 2023 Category: Biochemistry Authors: Damien Hall Source Type: research
