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Nutrition: Carbohydrates
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Adaptive laboratory evolution empowers Chlorella vulgaris strains for sustainable lipids and biomass overproduction for environmental applications
In this report, we demonstrated the adaptive laboratory evolution (ALE) by hypersaline conditions to improve the algal strains for increasing the lipid overproduction capacity of Chlorella vulgaris for environmental applications. The evolved strains (namely E2 and E2.5) without notable impairment in general physiological parameters were scrutinized after 35 cycles. Conventional gravimetric lipidomic analysis showed that total lipid accumulation was hiked by 2.2-fold in the ALE strains compared to the parental strains. Confocal observation of algal cells stained with Nile-red showed that the abundance of lipid droplets was ...
Source: Environmental Research - September 14, 2023 Category: Environmental Health Authors: Rajendran Varunraj Uthayakumar Priyadharshini Kannusamy Vijay Srinivasan Balamurugan Source Type: research
Characterizing dissolved organic matter and bacterial community interactions in a river network under anthropogenic landcover
In this study, synchronous fluorescence spectra (SFS) with Gaussian band fitting and two-dimensional correlation spectroscopy (2D-COS) were employed to identify DOM fractions and reveal their interactions with bacterial communities. DOM was extracted from a river network under eco-agricultural rural (RUR), eco-residential urban (URB), eco-economical town (TOW), and eco-industrial park (IND) regions in Jiashan Plain of eastern China. The overlapping peaks observed in the SFS were successfully separated into four fractions using Gaussian band fitting, i.e., tyrosine-like fluorescence (TYLF), tryptophan-like fluorescence (TRL...
Source: Environmental Research - September 14, 2023 Category: Environmental Health Authors: Shengqiang Tu Qingqian Li Zhangmu Jing Hongjie Gao Dongping Liu Meiqi Shao Huibin Yu Source Type: research
Biological properties of (1-4)-thio disaccharides
Carbohydr Res. 2023 Aug 31;533:108934. doi: 10.1016/j.carres.2023.108934. Online ahead of print.ABSTRACTThio sugars are carbohydrate derivatives in which one or more oxygen atoms have been replaced with sulfur. Thio sugars are effective inhibitors of glycosylases, have considerable therapeutic potential, and are used as drugs in the treatment of diabetes and infectious diseases. The development of this branch of carbohydrate chemistry would not be possible without the development of novel methods for its synthesis and the analysis of their biochemical properties. In this Review Article, we summarize our findings on the bio...
Source: Carbohydrate Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Anna Czubatka-Bie ńkowska Joanna Sarnik Tomasz Poplawski Source Type: research
Detailed investigation and influence of oxidation degree on synthesis, characterization and antibacterial activity of β- cyclodextrin
Carbohydr Res. 2023 Sep 9;533:108936. doi: 10.1016/j.carres.2023.108936. Online ahead of print.ABSTRACTOxidation of β-cyclodextrin (β-CD) using varying molar ratios of sodium periodate (NaIO4) was investigated in detail on synthesis, characterization and antibacterial property. Synthesis and characterization results showed that Oxidized β-cyclodextrins (OX-β-CDs) were obtained and aldehyde (CHO) groups were successfully introduced. Our results demonstrated that aldehyde content and yield increased with increasing NaIO4 molar amount. However, the structure of β-CD was degraded as a result of glycosidic ring opening wit...
Source: Carbohydrate Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Ayhan Bergal Muberra Andac Source Type: research
Biological properties of (1-4)-thio disaccharides
Carbohydr Res. 2023 Aug 31;533:108934. doi: 10.1016/j.carres.2023.108934. Online ahead of print.ABSTRACTThio sugars are carbohydrate derivatives in which one or more oxygen atoms have been replaced with sulfur. Thio sugars are effective inhibitors of glycosylases, have considerable therapeutic potential, and are used as drugs in the treatment of diabetes and infectious diseases. The development of this branch of carbohydrate chemistry would not be possible without the development of novel methods for its synthesis and the analysis of their biochemical properties. In this Review Article, we summarize our findings on the bio...
Source: Carbohydrate Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Anna Czubatka-Bie ńkowska Joanna Sarnik Tomasz Poplawski Source Type: research
Detailed investigation and influence of oxidation degree on synthesis, characterization and antibacterial activity of β- cyclodextrin
Carbohydr Res. 2023 Sep 9;533:108936. doi: 10.1016/j.carres.2023.108936. Online ahead of print.ABSTRACTOxidation of β-cyclodextrin (β-CD) using varying molar ratios of sodium periodate (NaIO4) was investigated in detail on synthesis, characterization and antibacterial property. Synthesis and characterization results showed that Oxidized β-cyclodextrins (OX-β-CDs) were obtained and aldehyde (CHO) groups were successfully introduced. Our results demonstrated that aldehyde content and yield increased with increasing NaIO4 molar amount. However, the structure of β-CD was degraded as a result of glycosidic ring opening wit...
Source: Carbohydrate Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Ayhan Bergal Muberra Andac Source Type: research
Exploring diet-induced promoter hypomethylation and PDK4 overexpression: implications for type 2 diabetes mellitus
CONCLUSIONS: These findings suggest that T2DM can be induced by high-carbohydrate and high-MSG diets. However, the sole consumption of high MSG did not lead to the development of T2DM. Further research should focus on conducting long-term studies to fully comprehend the impact of a high MSG diet on individuals with pre-existing T2DM.PMID:37707772 | DOI:10.1007/s11033-023-08794-7
Source: Molecular Biology Reports - September 14, 2023 Category: Molecular Biology Authors: Sulistyo Emantoko Dwi Putra Farizky Martriano Humardani Lisa Thalia Mulyanata Lady Theresa Adeodata Tanaya Heru Wijono Hikmawan Wahyu Sulistomo Dini Kesuma Risma Ikawaty Source Type: research
Total Energy Expenditure and Nutritional Intake in Continuous Multiday Ultramarathon Events
Int J Sport Nutr Exerc Metab. 2023 Sep 14:1-7. doi: 10.1123/ijsnem.2023-0063. Online ahead of print.ABSTRACTContinuous multiday ultramarathon competitions are increasingly popular and impose extreme energetic and nutritional demands on competitors. However, few data have been published on energy expenditure during these events. Here, we report doubly labeled water-derived measures of total energy expenditure (in kilocalories per day) and estimated physical activity level (PAL: total energy expenditure/basal metabolic rate) collected from five elite and subelite finishers (four males and one female, age 34.6 ± 4.9 years)-a...
Source: International Journal of Sport Nutrition and Exercise Metabolism - September 14, 2023 Category: Sports Medicine Authors: Andrew W Best Amanda McGrosky Zane Swanson Rebecca Rimbach Katie McConaughy Joe McConaughy Cara Ocobock Herman Pontzer Source Type: research
Analysis for Candida albicans in samples of intravenous lipids administered to premature infants with the goal of optimizing the use of the 1.2 µm filter
CONCLUSIONS: there was no presence of Candida albicans in the lipid solutions used directly with a 1.2 µm filter, however, the use of a single 1.2 µm filter in line "Y" is recommended according to international standards to save the health system.PMID:37705444 | DOI:10.20960/nh.04708
Source: Nutricion Hospitalaria - September 14, 2023 Category: Nutrition Authors: Haydee Villafana-Medina Ronaldo Quezada-P érez Jennifer Rodr íguez-Meza Julio Campos-Flori án Jorge V ásquez-Kool Carmen Mar ín-Tello Source Type: research
Optimizing carbohydrate intake from drugs in epileptic patients on a ketogenic diet
Nutr Hosp. 2023 Sep 1. doi: 10.20960/nh.04798. Online ahead of print.NO ABSTRACTPMID:37705443 | DOI:10.20960/nh.04798
Source: Nutricion Hospitalaria - September 14, 2023 Category: Nutrition Authors: Javier Coraz ón Villanueva Mar ía de la Torre Ortíz Virg ínia Puebla García Jos é Manuel Martínez Sesmero Source Type: research
Adaptive laboratory evolution empowers Chlorella vulgaris strains for sustainable lipids and biomass overproduction for environmental applications
In this report, we demonstrated the adaptive laboratory evolution (ALE) by hypersaline conditions to improve the algal strains for increasing the lipid overproduction capacity of Chlorella vulgaris for environmental applications. The evolved strains (namely E2 and E2.5) without notable impairment in general physiological parameters were scrutinized after 35 cycles. Conventional gravimetric lipidomic analysis showed that total lipid accumulation was hiked by 2.2-fold in the ALE strains compared to the parental strains. Confocal observation of algal cells stained with Nile-red showed that the abundance of lipid droplets was ...
Source: Environmental Research - September 14, 2023 Category: Environmental Health Authors: Rajendran Varunraj Uthayakumar Priyadharshini Kannusamy Vijay Srinivasan Balamurugan Source Type: research
Dietary intake in individuals with phenylketonuria: an integrative review
CONCLUSION: most individuals with PKU have low energy, protein and fiber intake, adequate lipid intake, and high carbohydrate intake. Metabolic control of the disease is still a challenge in all countries. Nutritional strategies to improve dietary nutritional composition and phenylalanine blood levels in individuals with PKU remain an urgent issue.PMID:37705455 | DOI:10.20960/nh.04579
Source: Nutricion Hospitalaria - September 14, 2023 Category: Nutrition Authors: Thais Regina Mezzomo Marcia Regina Messaggi Gomes Dias Tatiane Santos Rosana Marques Pereira Source Type: research
Analysis for Candida albicans in samples of intravenous lipids administered to premature infants with the goal of optimizing the use of the 1.2 µm filter
CONCLUSIONS: there was no presence of Candida albicans in the lipid solutions used directly with a 1.2 µm filter, however, the use of a single 1.2 µm filter in line "Y" is recommended according to international standards to save the health system.PMID:37705444 | DOI:10.20960/nh.04708
Source: Nutricion Hospitalaria - September 14, 2023 Category: Nutrition Authors: Haydee Villafana-Medina Ronaldo Quezada-P érez Jennifer Rodr íguez-Meza Julio Campos-Flori án Jorge V ásquez-Kool Carmen Mar ín-Tello Source Type: research
Optimizing carbohydrate intake from drugs in epileptic patients on a ketogenic diet
Nutr Hosp. 2023 Sep 1. doi: 10.20960/nh.04798. Online ahead of print.NO ABSTRACTPMID:37705443 | DOI:10.20960/nh.04798
Source: Nutricion Hospitalaria - September 14, 2023 Category: Nutrition Authors: Javier Coraz ón Villanueva Mar ía de la Torre Ortíz Virg ínia Puebla García Jos é Manuel Martínez Sesmero Source Type: research
In-depth profiling of carbohydrate isomers in biological tissues by chemical derivatization-assisted mass spectrometry imaging
In this study, we propose a high-sensitive and isomer-specific imaging approach to visualize the spatial distributions of monosaccharide and disaccharide isomers by integrating chemical derivatization and matrix-assisted laser desorption/ionization tandem mass spectrometry imaging (MALDI-MS2I). 2-Pyridinecarbohydrazide (PYD) is developed as a novel derivatization reagent which can not only improves the MS sensitivity of carbohydrates, but also enables the identification and visualization of ketose and aldose monosaccharide isomers, as well as linkage pattern, monosaccharide composition and anomeric configuration disacchari...
Source: Analytica Chimica Acta - September 14, 2023 Category: Chemistry Authors: Lei Wang Yuhao Han Yaqi Zhang Haoyuan Geng Zihan Zhu Panpan Chen Xiaoqing Cui Xiao Wang Chenglong Sun Source Type: research
