Probiotics, prebiotics, and synbiotics in childhood diarrhea
Braz J Med Biol Res. 2024 Apr 19;57:e13205. doi: 10.1590/1414-431X2024e13205. eCollection 2024.ABSTRACTAcute diarrhea is the second leading cause of morbidity and mortality attributed to infections in children under five years of age worldwide, with 1.7 million annual estimated cases and more than 500,000 deaths. Although hydroelectrolytic replacement is the gold standard in treating diarrhea, it does not interfere with the restoration of the intestinal microbiota. Several studies have searched for an adequate alternative in restructuring intestinal homeostasis, finding that treatments based on probiotics, prebiotics, and ...
Source: Braz J Med Biol Res - April 24, 2024 Category: Research Authors: E M S Martins L C Nascimento da Silva M S Carmo Source Type: research
Effect of whole-body vibration stimulation on plasma soluble TNF receptors in elderly with sarcopenia: a randomized controlled trial
In conclusion, an acute session of WBV influenced sTNFr2 levels, with sarcopenic individuals showing a greater effect. This suggested that WBV had a more pronounced impact on sTNFr2 in those with loss of muscle strength and/or physical performance. Additionally, WBV is gaining recognition as an efficient strategy for those with persistent health issues.PMID:38656072 | DOI:10.1590/1414-431X2024e13282 (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - April 24, 2024 Category: Research Authors: H C Almeida V K S Lage R Taiar J M Santos F A de Paula A Rapin D C S á-Caputo M Bernardo-Filho A C R Lacerda V A Mendon ça Source Type: research
Induced acute hyperglycemia modifies the barrier function of the intestinal epithelium by tissue inflammation and tight junction disruption resulting in hydroelectrolytic secretion in an animal model
This study aimed to evaluate the impact of acute hyperglycemia diabetes on intestinal tight junction proteins, metabolic failure, intestinal ion and water transports, and IEBF parameters. Diabetes was induced in male Rattus norvegicus (200-310 g) with 0.5 mL of streptozotocin (70 mg/kg). Glycemic and clinical parameters were evaluated every 7 days, and intestinal parameters were evaluated on the 14th day. The MetS-D animals showed a clinical pattern of hyperglycemia, with increases in the area of villi and crypts, lactulose:mannitol ratio, myeloperoxidase (MPO) activity, and intestinal tissue concentrations of malondialdeh...
Source: Braz J Med Biol Res - April 24, 2024 Category: Research Authors: F J W S Siqueira F A P Rodrigues S A Ribeiro H N Veras F C S Ferreira R C L Siqueira A A Dos Santos A Havt A A M Lima Source Type: research
Integrative analyses of genetic characteristics associated with skeletal endothelial cells
In this study, we conducted a systematic analysis of differentially expressed genes (DEGs) and the associated pathways in juvenile and mature femoral ECs, utilizing data sourced from the Gene Expression Omnibus (GEO) repositories (GSE148804) and employing bioinformatics tools. Through this approach, we successfully discerned six pivotal genes, namely Adamts1, Adamts2, Adamts4, Adamts14, Col5a1, and Col5a2. Subsequently, we constructed a miRNA-mRNA network based on miRNAs displaying differential expression between CD31hiEMCNhi and CD31lowEMCNlow ECs, utilizing online repositories for prediction. The expression of miR-466i-3...
Source: Braz J Med Biol Res - April 24, 2024 Category: Research Authors: Zhanhui Wang Bowen Hu Xiaoming Chen Zheng Zhang Lu Liu Nan Li Chun Liang Source Type: research
Development and validation of a clinical prediction model for prognostic factors in patients with primary pontine hemorrhage
Braz J Med Biol Res. 2024 Apr 19;57:e13359. doi: 10.1590/1414-431X2024e13359. eCollection 2024.ABSTRACTWe aimed to develop a prognostic model for primary pontine hemorrhage (PPH) patients and validate the predictive value of the model for a good prognosis at 90 days. A total of 254 PPH patients were included for screening of the independent predictors of prognosis, and data were analyzed by univariate and multivariable logistic regression tests. The cases were then divided into training cohort (n=219) and validation cohort (n=35) based on the two centers. A nomogram was developed using independent predictors from the train...
Source: Braz J Med Biol Res - April 24, 2024 Category: Research Authors: Anquan Hu Heyan Qin Shina Wu Xiaolin Zhao Yumeng Li Feng Chen Tao Liu Source Type: research
Could nanotechnology improve exercise performance? Evidence from animal studies
In conclusion, nutritional nanocompounds are a promising approach to improving exercise performance. As the studies using nutritional nanocompounds were all conducted in animals, further studies in humans are necessary to better understand the application of nutritional nanocompounds in sport and exercise science.PMID:38656076 | DOI:10.1590/1414-431X2024e13360 (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - April 24, 2024 Category: Research Authors: M R Lima B J Moreira R Bertuzzi A E Lima-Silva Source Type: research
Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis
Braz J Med Biol Res. 2024 Mar 18;57:e12951. doi: 10.1590/1414-431X2024e12951. eCollection 2024.ABSTRACTThe incidences of periodontitis and osteoporosis are rising worldwide. Observational studies have shown that periodontitis is associated with increased risk of osteoporosis. We performed a Mendelian randomization (MR) study to genetically investigate the causality of periodontitis on osteoporosis. We explored the causal effect of periodontitis on osteoporosis by MR analysis. A total of 9 single nucleotide polymorphisms (SNP) were related to periodontitis. The primary approach in this MR analysis was the inverse variance-w...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jiale Wu Lihui Yao Yuchen Liu ShuaiShuai Zhang Kan Wang Source Type: research
Temporal evolution of postsurgical bone repair in a rabbit model: A [99mTc]Tc-MDP scintigraphic study
This study used bone scintigraphy to evaluate osteogenesis secondary to osteotomy in a preclinical model of New Zealand rabbits. For this purpose, we conducted a longitudinal, prospective, case-control study in which scintigraphic variables were measured in both the right forearm (case-operated) and the left forearm (control - non-operated). The study sample consisted of 10 rabbits subjected to osteotomy, followed by a 12-week postoperative evaluation period, divided into six imaging stages at 1, 2, 3, 4, 8, and 12 weeks. We observed that the operated forearm showed significantly higher external radiation than the control ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: A Yoneda K J C C de Lacerda L Alexandre-Santos E N Itikawa P Louzada-Junior L Wichert-Ana Source Type: research
Serum autophagy-related gene 5 level in stroke patients: correlation with CD4+ T cells and cognition impairment during a 3-year follow-up
This study aimed to explore the correlation of serum ATG5 with CD4+ T cells and cognition impairment in stroke patients. Peripheral blood was collected from 180 stroke patients for serum ATG5 and T helper (Th) 1, Th2, Th17, and regulatory T (Treg) cell detection via enzyme-linked immunosorbent assays and flow cytometry. The Mini-Mental State Examination (MMSE) scale was completed at enrollment, year (Y)1, Y2, and Y3 in stroke patients. Serum ATG5 was also measured in 50 healthy controls (HCs). Serum ATG5 was elevated in stroke patients compared to HCs (P<0.001) and was positively correlated to Th2 cells (P=0.022), Th17 ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Juanjuan Qu Linxia Wu Meng Zhang Minchen Kan Huimin Chen Yanqing Shi Shuangyu Wang Xiaohua Wang Fan Chen Source Type: research
Upregulation of PGC-1 α expression by pioglitazone mediates prevention of sepsis-induced acute lung injury
This study aimed to investigate the role of the PGC-1α agonist pioglitazone (PGZ) in modulating sepsis-induced ALI. A mouse model of sepsis-induced ALI was established using cecal ligation and puncture (CLP). An in vitro model was created by stimulating MH-S cells with lipopolysaccharide (LPS). qRT-PCR was used to measure mRNA levels of M1 markers iNOS and MHC-II and M2 markers Arg1 and CD206 to evaluate macrophage polarization. Western blotting detected expression of peroxisome proliferator-activated receptor gamma (PPARγ) PGC-1α, and mitochondrial biogenesis proteins NRF1, NRF2, and mtTFA. To assess mitochondrial cont...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jing Tang Wenzhu Dong Dan Wang Qin Deng Honggang Guo Guibao Xiao Source Type: research
Silencing of matrix metalloprotease-12 delays the progression of castration-resistant prostate cancer by regulating autophagy and lipolysis
In this study, we observed the effect of MMP-12 on the progression of CRPC and further explored its potential mechanism of action. High levels of MMP-12 were observed in patients with CRPC. We therefore developed cell co-culture and mouse models to study the function of MMP-12. Silencing MMP-12 in CRPC cells disrupted lipid utilization and autophagy marker expression via the CD36/CPT1 and P62/LC3 pathways, respectively, leading to reduced CRPC cell migration and invasion. Moreover, animal experiments confirmed that MMP-12-knockdown CRPC xenograft tumors exhibited reduced tumor growth, and the mechanisms involved the promot...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Xiaoyu Zheng Xiaoqin Xie Wei Wang Liang Wang Bing Tan Source Type: research
Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis
Braz J Med Biol Res. 2024 Mar 18;57:e12951. doi: 10.1590/1414-431X2024e12951. eCollection 2024.ABSTRACTThe incidences of periodontitis and osteoporosis are rising worldwide. Observational studies have shown that periodontitis is associated with increased risk of osteoporosis. We performed a Mendelian randomization (MR) study to genetically investigate the causality of periodontitis on osteoporosis. We explored the causal effect of periodontitis on osteoporosis by MR analysis. A total of 9 single nucleotide polymorphisms (SNP) were related to periodontitis. The primary approach in this MR analysis was the inverse variance-w...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jiale Wu Lihui Yao Yuchen Liu ShuaiShuai Zhang Kan Wang Source Type: research
Temporal evolution of postsurgical bone repair in a rabbit model: A [99mTc]Tc-MDP scintigraphic study
This study used bone scintigraphy to evaluate osteogenesis secondary to osteotomy in a preclinical model of New Zealand rabbits. For this purpose, we conducted a longitudinal, prospective, case-control study in which scintigraphic variables were measured in both the right forearm (case-operated) and the left forearm (control - non-operated). The study sample consisted of 10 rabbits subjected to osteotomy, followed by a 12-week postoperative evaluation period, divided into six imaging stages at 1, 2, 3, 4, 8, and 12 weeks. We observed that the operated forearm showed significantly higher external radiation than the control ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: A Yoneda K J C C de Lacerda L Alexandre-Santos E N Itikawa P Louzada-Junior L Wichert-Ana Source Type: research
Serum autophagy-related gene 5 level in stroke patients: correlation with CD4+ T cells and cognition impairment during a 3-year follow-up
This study aimed to explore the correlation of serum ATG5 with CD4+ T cells and cognition impairment in stroke patients. Peripheral blood was collected from 180 stroke patients for serum ATG5 and T helper (Th) 1, Th2, Th17, and regulatory T (Treg) cell detection via enzyme-linked immunosorbent assays and flow cytometry. The Mini-Mental State Examination (MMSE) scale was completed at enrollment, year (Y)1, Y2, and Y3 in stroke patients. Serum ATG5 was also measured in 50 healthy controls (HCs). Serum ATG5 was elevated in stroke patients compared to HCs (P<0.001) and was positively correlated to Th2 cells (P=0.022), Th17 ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Juanjuan Qu Linxia Wu Meng Zhang Minchen Kan Huimin Chen Yanqing Shi Shuangyu Wang Xiaohua Wang Fan Chen Source Type: research
Upregulation of PGC-1 α expression by pioglitazone mediates prevention of sepsis-induced acute lung injury
This study aimed to investigate the role of the PGC-1α agonist pioglitazone (PGZ) in modulating sepsis-induced ALI. A mouse model of sepsis-induced ALI was established using cecal ligation and puncture (CLP). An in vitro model was created by stimulating MH-S cells with lipopolysaccharide (LPS). qRT-PCR was used to measure mRNA levels of M1 markers iNOS and MHC-II and M2 markers Arg1 and CD206 to evaluate macrophage polarization. Western blotting detected expression of peroxisome proliferator-activated receptor gamma (PPARγ) PGC-1α, and mitochondrial biogenesis proteins NRF1, NRF2, and mtTFA. To assess mitochondrial cont...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jing Tang Wenzhu Dong Dan Wang Qin Deng Honggang Guo Guibao Xiao Source Type: research
