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Total 135484 results found since Jan 2013.

Effect of HIF-1 αsiRNA-linked AuNRs on radiotherapy of nasopharyngeal carcinoma.
Effect of HIF-1αsiRNA-linked AuNRs on radiotherapy of nasopharyngeal carcinoma. Cell Mol Biol (Noisy-le-grand). 2020 Jul 31;66(5):185-190 Authors: Xu G, Zhang H, Li Z, Wu S, Quan R, Mao K, Sheng Y, Li X Abstract The radiation sensitivity of tumor cells is closely related to tumor cell hypoxia. Hypoxia-inducible factor-1α (HIF-1α) is considered a key transcription factor which regulates the sensitivity of hypoxic tumor cells to radiotherapy. On the other hand, some studies have shown that gold nanomaterials improve radiation sensitivity. However, studies on the effect of gold nanomaterials carrying H...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Effects of sterol derivatives in cationic liposomes on  biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes
Mol Med Rep. 2021 Aug;24(2):598. doi: 10.3892/mmr.2021.12237. Epub 2021 Jun 24.ABSTRACTCationic liposomes can be intravenously injected to deliver short interfering (si)RNAs into the lungs. The present study investigated the effects of sterol derivatives in systemically injected siRNA/cationic liposome complexes (siRNA lipoplexes) on gene‑knockdown in the lungs of mice. Cationic liposomes composed of 1,2‑dioleoyl‑3‑trimethylammonium‑propane or dimethyldioctadecylammonium bromide (DDAB) were prepared as a cationic lipid, with sterol derivatives such as cholesterol (Chol), β‑sitosterol, ergosterol (Ergo) or stig...
Source: Molecular Medicine Reports - June 24, 2021 Category: Molecular Biology Authors: Yoshiyuki Hattori Hiromu Saito Teruaki Oku Kei-Ichi Ozaki Source Type: research

Groove modification of siRNA duplex to elucidate siRNA-protein interactions using 7-bromo-7-deazaadenosine and 3-bromo-3-deazaadenosine as chemical probes
In this study, the logical chemical probes,... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - September 21, 2016 Category: Molecular Biology Authors: Noriko Saito Tarashima Source Type: research

Groove modification of siRNA duplexes to elucidate siRNA-protein interactions using 7-bromo-7-deazaadenosine and 3-bromo-3-deazaadenosine as chemical probes
Org. Biomol. Chem., 2016, Advance Article DOI: 10.1039/C6OB01866A, PaperNoriko Saito-Tarashima, Hirotaka Kira, Tomoya Wada, Kazuya Miki, Shiho Ide, Naoshi Yamazaki, Akira Matsuda, Noriaki Minakawa The chemical probes, comprising Br7C7A and Br3C3A, to investigate RNA-protein interactions were developed. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - September 28, 2016 Category: Molecular Biology Authors: Noriko Saito-Tarashima Source Type: research

High-Content siRNA Screen of the Kinome Identifies Kinases Involved in Git2-Induced Mesenchymal-Epithelial Transition.
Abstract Epithelial-mesenchymal transition (EMT) and its reverse process mesenchymal-epithelial transition (MET) programs are involced in the metastatic process. More and more evidence confirms that EMT is vital for the initiation and dissemination of cancer cells whereas MET is critical for successful metastatic colonization of a secondary organ. The regulating mechanism of EMT mediated cancer progression and metastasis has been deeply investigated. However, what processes are dependent on MET in metastatic cascades remains unclear. Here, we created a cell based high-content siRNA screen using the breast cancer c...
Source: Molekuliarnaia Biologiia - July 1, 2017 Category: Molecular Biology Authors: Cao MG, Xu J, Yang QF, Guo ZP, Zhang KB, Li XB, Wu SQ, Zhou W Tags: Mol Biol (Mosk) Source Type: research

Non-targeting siRNA-mediated responses are associated with apoptosis in chemotherapy-induced senescent skin cancer cells
Chem Biol Interact. 2022 Nov 4:110254. doi: 10.1016/j.cbi.2022.110254. Online ahead of print.ABSTRACTIt is widely accepted that siRNA transfection can promote some off-target effects in the genome; however, little is known about how the cells can respond to the presence of non-viral dsRNA. In the present study, non-targeting control siRNA (NTC-siRNA) was used to evaluate its effects on the activity of pathogen and host-derived nucleic acid-associated signaling pathways such as cGAS-STING, RIG-I, MDA5 and NF-κB in A431 skin cancer cells and BJ fibroblasts. NTC-siRNA treatment promoted cytotoxicity in cancer cells. Furtherm...
Source: Chemico-Biological Interactions - November 7, 2022 Category: Molecular Biology Authors: Gabriela Betlej Dominika B łoniarz Anna Lewi ńska Maciej Wnuk Source Type: research

Where should siRNAs go: applicable organs for siRNA drugs
Exp Mol Med. 2023 Jul 10. doi: 10.1038/s12276-023-00998-y. Online ahead of print.ABSTRACTRNA interference mediated by small interfering RNAs (siRNAs) has been exploited for the development of therapeutics. siRNAs can be a powerful therapeutic tool because the working mechanisms of siRNAs are straightforward. siRNAs determine targets based on their sequence and specifically regulate the gene expression of the target gene. However, efficient delivery of siRNAs to the target organ has long been an issue that needs to be solved. Tremendous efforts regarding siRNA delivery have led to significant progress in siRNA drug developm...
Source: Molecular Medicine - July 10, 2023 Category: Molecular Biology Authors: Insook Ahn Chanhee S Kang Jinju Han Source Type: research

siRNA Therapeutics for Protein Misfolding Diseases of the Central Nervous System
Methods Mol Biol. 2021;2282:377-394. doi: 10.1007/978-1-0716-1298-9_20.ABSTRACTNanoparticles have been used to deliver siRNA to tissues and cells to silence specific genes in diverse organisms. Research and clinical application of nanoparticles like liposomes for drug delivery requires targeting them to specific anatomic regions or cell types, while avoiding off-target effects or clearance by the liver, kidney, or the immune system. Delivery to the central nervous system (CNS) presents additional challenges to cross the blood-brain barrier (BBB) to specific cell types like neurons, astrocytes, or glia. Here, we describe th...
Source: Mol Biol Cell - April 30, 2021 Category: Molecular Biology Authors: Mark D Zabel Luke Mollnow Heather Bender Source Type: research

Radiosensitizing effects of curcumin alone or combined with GLUT1 siRNA on laryngeal carcinoma cells through AMPK pathway-induced autophagy
In this study, we investigated the ability of curcumin alone or in combination with GLUT1 siRNA to radiosensitize laryngeal carcinoma (LC) through the induction of autophagy. Protein levels in tumour tissues and LC cells were measured by immunohistochemistry and Western blotting. In vitro, cell proliferation, colony formation assays, cell death and autophagy were detected. A nude mouse xenograft model was established through the injection of Tu212 cells. We found that GLUT1 was highly expressed and negatively associated with autophagy-related proteins in LC and that curcumin suppressed radiation-mediated GLUT1 overexpressi...
Source: J Cell Mol Med - May 6, 2021 Category: Molecular Biology Authors: Li-Bo Dai Jiang-Tao Zhong Li-Fang Shen Shui-Hong Zhou Zhong-Jie Lu Yang-Yang Bao Jun Fan Source Type: research