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Specialty: Urology & Nephrology

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Total 430 results found since Jan 2013.

Dual inhibition by S6K1 and Elf4E is essential for controlling cellular growth and invasion in bladder cancer
Conclusion: These findings suggest that both the mTOR pathway downstream of eukaryotic initiation factor 4E and S6K1 can be successfully inhibited, therefore, the recurrence of bladder cancer can be prevented by high-dose rapamycin only, suggesting that 4E-BP1 might be still under mTORC1.
Source: Urologic Oncology: Seminars and Original Investigations - November 18, 2013 Category: Urology & Nephrology Authors: Jong Kyou Kwon, Soon-Ja Kim, Jung Hoon Kim, Kyung Mee Lee, In Ho Chang Tags: Abstracts for Online Original Articles and Reviews Source Type: research

The Vav3 oncogene enhances the malignant potential of prostate cancer cells under chronic hypoxia
Conclusions: Our results demonstrate that Vav3 plays a crucial role in prostate cancer growth and malignant behavior, thus revealing a novel potential therapeutic target.
Source: Urologic Oncology: Seminars and Original Investigations - February 11, 2013 Category: Urology & Nephrology Authors: Kenichi Hirai, Takeo Nomura, Mutsushi Yamasaki, Toru Inoue, Takahiro Narimatsu, Ph.D. Chisato Nakada, Ph.D. Yoshiyuki Tsukamoto, Keiko Matsuura, Fuminori Sato, Masatsugu Moriyama, Hiromitsu Mimata Tags: Clinical - Prostate Source Type: research

Phosphorylated p70S6K in noninvasive low-grade urothelial carcinoma of the bladder: correlation with tumor recurrence.
Abstract We investigated whether inhibiting phosphorylated p70S6K (p-p70S6K) suppresses the proliferation and growth of noninvasive low-grade urothelial carcinoma (LG-URCa) in vitroand whether p-p70S6K can serve as a predictive biomarker for the recurrence of noninvasive LG-URCa of the bladder in patients. We constructed a tissue microarray (TMA) for 95 LG-URCa and 35 benign urothelium samples and performed immunohistochemical staining for p-p70S6K and p-4E-BP1. A Cox regression model was used to investigate the predictive factors for recurrence of LG-URCa. We investigated the dose-dependent antiproliferative effe...
Source: Asian Journal of Andrology - March 11, 2014 Category: Urology & Nephrology Authors: Kim SJ, Kim JH, Jung HS, Lee TJ, Lee KM, Chang IH Tags: Asian J Androl Source Type: research

REGULATION OF (PRO)RENIN RECEPTOR EXPRESSION IN mIMCD VIA GSK3β-NFAT5- SIRT-1 SIGNALING PATHWAY.
Abstract The localization and regulation of (Pro)renin receptor expression (PRR) in the kidney collecting duct cells is not well established. We hypothesized that low salt contributes to the regulation of PRR expression in these cells via GSK3β-NFAT5-SIRT-1 signaling pathway. mIMCD were treated with NaCl at 130mM (NS), 63mM (LS) or 209mM (HS) alone or in combination with NFAT5 scrambled siRNA, NFAT5 siRNA, or the SIRT-1 inhibitor, EX527. Compared to NS, LS increased the mRNA and protein expression of PRR by 71% and 69% (P< 0.05), and reduced phosphorylation of GSK3β by 62% (P< 0.01), mRNA and protein expre...
Source: Am J Physiol Renal P... - July 2, 2014 Category: Urology & Nephrology Authors: Quadri S, Siragy HM Tags: Am J Physiol Renal Physiol Source Type: research

Role of caveolin 1 in AT1a receptor-mediated uptake of angiotensin II in the proximal tubule of the kidney.
Abstract Caveolin 1 (CAV-1) functions not only as a constitutive scaffolding protein of caveolae but also as a vesicular transporter and signaling regulator. In the present study, we tested the hypothesis that CAV-1 knockout (CAV-1 KO) inhibits AT1 (AT1a) receptor-mediated uptake of angiotensin II (ANG II) in the proximal tubule and attenuates blood pressure responses in ANG II-induced hypertension. To determine the role of CAV-1 in mediating the uptake of fluorescein (FITC)-labeled ANG II, wild-type (WT) mouse PT cells (mPCT) were transfected with CAV-1 siRNA for 48 h before AT1a-mediated uptake of FITC-ANG II wa...
Source: Am J Physiol Renal P... - August 27, 2014 Category: Urology & Nephrology Authors: Li XC, Gu V, Miguel-Qin E, Zhuo JL Tags: Am J Physiol Renal Physiol Source Type: research

The effect of FoxO1 on the proliferation of rat mesangial cells under high glucose conditions
Conclusions Overexpression of FoxO1 caused upregulation of p27, which promoted cell cycle arrest and inhibited hyperproliferation of MCs induced by HG. Degradation of FoxO1 caused an increase in p27 and stimulated MC proliferation. These findings unveil part of the molecular mechanism of FoxO1 regulation of MC hyperproliferation induced by HG.
Source: Nephrology Dialysis Transplantation - September 24, 2014 Category: Urology & Nephrology Authors: Liu, F., Ma, X.-J., Wang, Q.-Z., Zhao, Y.-Y., Wu, L.-N., Qin, G.-J. Tags: BASIC SCIENCE Source Type: research

Effects of luteinizing hormone receptor signaling in prostate cancer cells
CONCLUSIONCollectively, these data demonstrate that silencing LHR expression suppresses androgen synthesis and signaling and the LH‐LHR pathway may represent a viable therapeutic strategy in PC. Prostate © 2014 Wiley Periodicals, Inc.
Source: The Prostate - November 12, 2014 Category: Urology & Nephrology Authors: Shigang Xiong, Qingcai Wang, Stephen V. Liu, Robert B. Montgomery, Frank Z. Stanczyk, John G. Vallone, Noah M. Merin, Jacek Pinski Tags: Original Article Source Type: research

Cyclic stretch induced TGF-β1 and fibronectin expression is mediated by β1 integrin through c-Src- and STAT3- dependent pathways in renal epithelial cells.
In this study, we investigated the mechanism by which mechanical stretch, an in vitro model for chronic obstructive nephropathy, regulates fibronectin and transforming growth factor-β1 (TGF-β1) expression in cultured human proximal tubular epithelium (HK-2) cells. Mechanical stretch up-regulated fibronectin and TGF-β1 expression and activated STAT3 in a time-dependent manner. Stretch-induced fibronectin and TGF-β1 were suppressed by a STAT3 inhibitor, S3I-201 and by small interfering RNA (siRNA) targeting human STAT3 (STAT3 siRNA). Similarly, fibronectin and TGF-β1 expression and STAT3 activation induced by mechanical...
Source: Am J Physiol Renal P... - December 4, 2014 Category: Urology & Nephrology Authors: Hamzeh MT, Sridhara R, Alexander LD Tags: Am J Physiol Renal Physiol Source Type: research

CYP3A5 regulates prostate cancer cell growth by facilitating nuclear translocation of AR
CONCLUSIONAs CYP3A5 regulates the nuclear translocation of AR; co‐targeting CYP3A5 may provide a novel strategy for enhancing the efficacy of androgen deprivation therapy. Consequentially, these data suggest that concomitant medications may impact androgen deprivation therapy's efficacy. Prostate © 2015 Wiley Periodicals, Inc.
Source: The Prostate - January 13, 2015 Category: Urology & Nephrology Authors: Ranjana Mitra, Oscar B. Goodman Tags: Original Article Source Type: research

Clusterin produced by Sertoli cells inhibits heat stress‐induced apoptosis in the rat testis
The objectives of this study were to determine whether the inhibition of clusterin expression in rat Sertoli cells enhances heat stress‐induced apoptosis. The scrotums of rats were immersed in a water bath of 43 °C for 15 min. Testicular weight and germ cell number markedly decreased after the heat treatment in a time‐dependent manner. In contrast, clusterin mRNA and protein expression levels were significantly up‐regulated and peaked on day 21. The apoptotic index was markedly increased 1 day after the heat treatment. We then purified Sertoli cells from the rat testes, and an expression vector containing siRNA t...
Source: Andrologia - February 8, 2015 Category: Urology & Nephrology Authors: K. Matsushita, H. Miyake, K. Chiba, M. Fujisawa Tags: Original Article Source Type: research

WNK1 Activates Large-Conductance Ca2+-Activated K+ Channels through Modulation of ERK1/2 Signaling
In this study, we investigated the effect of WNK1 on BK channel activity. In HEK293 cells stably expressing the α subunit of BK (HEK-BKα cells), siRNA-mediated knockdown of WNK1 expression significantly inhibited both BKα channel activity and open probability. Knockdown of WNK1 expression also significantly inhibited BKα protein expression and increased ERK1/2 phosphorylation, whereas overexpression of WNK1 significantly enhanced BKα expression and decreased ERK1/2 phosphorylation in a dose-dependent manner in HEK293 cells. Knockdown of ERK1/2 prevented WNK1 siRNA-mediated inhibition of BK&alp...
Source: Journal of the American Society of Nephrology : JASN - March 31, 2015 Category: Urology & Nephrology Authors: Liu, Y., Song, X., Shi, Y., Shi, Z., Niu, W., Feng, X., Gu, D., Bao, H.-F., Ma, H.-P., Eaton, D. C., Zhuang, J., Cai, H. Tags: Basic Research Source Type: research

High glucose promotes TGF-β1 production by inducing FOS expression in human peritoneal mesothelial cells
Conclusions FOS is a downstream effector of HG stimulation in HPMCs that contributes to TGF-β1 production, suggesting that blocking FOS expression may be a therapeutic target for peritoneal fibrosis.
Source: Clinical and Experimental Nephrology - May 28, 2015 Category: Urology & Nephrology Source Type: research

Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro.
Abstract The Sertoli cell tight junction (TJ) is the key component of the blood-testis barrier, where it sequesters developing germ cells undergoing spermatogenesis within the seminiferous tubules. Hormonally regulated claudin-11 is a critical transmembrane protein involved in barrier function and its murine knockout results in infertility. We aimed to assess quantitatively the significance of the contribution of claudin-11 to TJ function, in vitro, using siRNA-mediated gene silencing. We also conducted an analysis of the contribution of occludin, another intrinsic transmembrane protein of the TJ. Silencing of cla...
Source: Asian Journal of Andrology - November 10, 2015 Category: Urology & Nephrology Authors: McCabe MJ, Foo CF, Dinger ME, Smooker PM, Stanton PG Tags: Asian J Androl Source Type: research

Mechanisms of bradykinin-induced expression of connective tissue growth factor and nephrin in podocytes
Diabetic nephropathy (DN) is the main cause of morbidity and mortality in diabetes and is characterized by mesangial matrix deposition and podocytopathy, including podocyte loss. The risk factors and mechanisms involved in the pathogenesis of DN are still not completely defined. In the present study, we aimed to understand the cellular mechanisms through which activation of B2 kinin receptors contribute to the initiation and progression of DN. Stimulation of cultured rat podocytes with bradykinin (BK) resulted in a significant increase in ROS generation, and this was associated with a significant increase in NADPH oxidase ...
Source: AJP: Renal Physiology - December 1, 2015 Category: Urology & Nephrology Authors: Msallem, J. A., Chalhoub, H., Al-Hariri, M., Saad, L., Jaffa, M. A., Ziyadeh, F. N., Jaffa, A. A. Tags: ARTICLES Source Type: research

NF-κB-dependent increase in tissue factor expression is responsible for hypoxic podocyte injury
Conclusion Hypoxia increased the expression of TF in human podocytes NF-κB dependently. TF may have a critical role in the hypoxic podocyte injury.
Source: Clinical and Experimental Nephrology - December 29, 2015 Category: Urology & Nephrology Source Type: research