Filtered By:
Specialty: Allergy & Immunology
This page shows you your search results in order of relevance. This is page number 12.
Order by Relevance | Date
Total 1296 results found since Jan 2013.
Inhibition of B lymphocyte-induced maturation protein-1 reduces the production of autoantibody and alleviates symptoms of systemic lupus erythematosus.
This study aimed to investigate the role of Blimp-1 in the development of SLE and autoimmune-like symptoms. Lentivirus-mediated Blimp-1 siRNA was constructed and injected into MRL-Fas(lpr) lupus mice. The expression levels of Blimp-1, J-chain, C-myc, XBP-1 and BCMA in peripheral blood mononuclear cells (PMBCs) were determined by RT-PCR. Anti-dsDNA autoantibody levels were detected using ELISA. The expression levels of Blimp-1 in liver, kidney, spleen and lymph nodes of mice were also detected by Western blot. The 24-h urinary protein was monitored weekly. Our results demonstrated that in MRL-Fas(lpr) lupus mice, Blimp-1 wa...
Source: Autoimmunity - February 17, 2016 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research
Regulating the expression of CD80/CD86 on dendritic cells to induce immune tolerance after xeno-islet transplantation.
CONCLUSIONS: CD86-silenced-DCs induced immune tolerance of rat xeno-islets in recipient diabetic mice with up-regulated peripheral blood CD4(+)CD25(+)T cells.
PMID: 26879762 [PubMed - as supplied by publisher]
Source: Immunobiology - February 3, 2016 Category: Allergy & Immunology Authors: Ke N, Su A, Huang W, Szatmary P, Zhang Z Tags: Immunobiology Source Type: research
Emodin suppresses LPS-induced inflammation in RAW264.7 cells through a PPARγ-dependent pathway.
Abstract
Inflammation is a defense and protective response to multiple harmful stimuli. Over and uncontrolled inflammation can lead to local tissues or even systemic damages and injuries. Actually, uncontrolled and self-amplified inflammation is the fundament of the pathogenesis of a variety of inflammatory diseases, including sepsis shock, acute lung injury and acute respiratory distress syndrome (ALI/ARDS). Our recent study showed that emodin, the main active component of Radix rhizoma Rhei, could significantly ameliorate LPS-induced ALI/ARDS in mice. However, its underlying signal pathway was not still very cle...
Source: International Immunopharmacology - February 21, 2016 Category: Allergy & Immunology Authors: Zhu T, Zhang W, Feng SJ, Yu HP Tags: Int Immunopharmacol Source Type: research
Role of PHLPP1 in inflammation response: Its loss contributes to gliomas development and progression.
Abstract
PH domain leucine-rich repeats protein phosphatase 1(PHLPP1) belongs to a novel family of Ser/Thr protein phosphatases: PHLPP serves as tumor suppressor in several cancers. However, little knowledge about the expression of PHLPP1 in human glioma tumor tissue and its role in inflammation response in glioma cells was known. Glioma samples were obtained from a total of 37 patients including 16 males and 21 females with surgical removal of the brain tumor. PHLPP1 protein and inflammatory cytokines were measured by Western blot analysis and immunohistochemistry while mRNA was determined by RT-PCR. The levels o...
Source: International Immunopharmacology - March 10, 2016 Category: Allergy & Immunology Authors: Teng DC, Sun J, An YQ, Hu ZH, Liu P, Ma YC, Han B, Shi Y Tags: Int Immunopharmacol Source Type: research
SNAIL gene inhibited by hypoxia-inducible factor 1α (HIF-1α) in epithelial ovarian cancer.
Authors: Zhang P, Liu Y, Feng Y, Gao S
Abstract
The aim of this study was to investigate the relationship between HIF-1α and SNAIL gene expression in the epithelial ovarian cancer (EOC) cell line. EOC cells were treated with hypoxia, hypoxia combined with rapamycin, and control. The expression of HIF-1α and E-cad were assessed by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. The gene expression of SNAIL was studied by RT-PCR and real-time PCR. RNA interference technology was used to determine the relationship between HIF-1α and SNAIL. The present study indicated that the HIF-1α ...
Source: International Journal of Immunopathology and Pharmacology - April 6, 2016 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research
Enhanced p62 Is Responsible for Mitochondrial Pathway-Dependent Apoptosis and Interleukin-1β Production at the Early Phase by Monosodium Urate Crystals in Murine Macrophage
Abstract
The aim of this study was to clarify the role of p62-dependent mitochondrial apoptosis in the initiation of monosodium urate (MSU) crystal-induced inflammation in macrophages. The induction of mitochondrial apoptosis in RAW 264.7 murine macrophages by MSU crystals was measured using western blotting and quantitative real-time polymerase chain reaction for Bax, caspase-3, caspase-9, or PARP1, and by flow cytometric analysis. Immunoprecipitation and western blotting was applied to detect ubiquitination of p62, TRAF6, and caspase-9. Mitochondrial apoptosis, reactive oxygen species (ROS) generation, and cell ...
Source: Inflammation - June 6, 2016 Category: Allergy & Immunology Source Type: research
Cold-inducible RNA-binding protein mediates cold air inducible airway mucin production through TLR4/NF-κB signaling pathway.
In this study, we sought to explore the expression of CIRP within bronchial biopsy specimens, the effect on mucin5AC (MUC5AC) production in chronic inflammatory airway diseases and the potential signaling pathways involved in cold air stimulation process. We found that CIRP protein expression was significantly increased in patients with COPD and in mice treated with cold air. Moreover, cold air stimulation induced MUC5AC expression in wild-type mice but not in CIRP(-/-) mice. In vitro, cold air stress significantly elevated the transcriptional and protein expression levels of MUC5AC in human bronchial epithelial cells. CIR...
Source: International Immunopharmacology - July 12, 2016 Category: Allergy & Immunology Authors: Chen L, Ran D, Xie W, Xu Q, Zhou X Tags: Int Immunopharmacol Source Type: research
PGRN Suppresses Inflammation and Promotes Autophagy in Keratinocytes Through the Wnt/β-Catenin Signaling Pathway
Abstract
Psoriasis is a chronic, immune-mediated inflammatory skin disease that has a major impact on patients’ quality of life. Progranulin (PGRN) is highly expressed in skin diseases and plays an important role in inflammation response and autophagy. However, the function of PGRN in the immune system and autophagy in psoriasis has not been clearly identified and elaborated on. Thus, this study aimed to investigate the role of PGRN on the inflammatory and autophagy process underlying inflammation in HaCaT cells. We showed that PGRN was markedly highly expressed in psoriasis lesions and inflammatory HaCaT cells...
Source: Inflammation - July 20, 2016 Category: Allergy & Immunology Source Type: research
PGRN Suppresses Inflammation and Promotes Autophagy in Keratinocytes Through the Wnt/ β-Catenin Signaling Pathway
Abstract Psoriasis is a chronic, immune-mediated inflammatory skin disease that has a major impact on patients ’ quality of life. Progranulin (PGRN) is highly expressed in skin diseases and plays an important role in inflammation response and autophagy. However, the function of PGRN in the immune system and autophagy in psoriasis has not been clearly identified and elaborated on. Thus, this study aimed to investigate the role of PGRN on the inflammatory and autophagy process underlying inflammation in HaCaT cells. We showed that PGRN was markedly highly expressed in psoriasis lesions and inflammatory HaCaT cells. Specif...
Source: Inflammation - July 21, 2016 Category: Allergy & Immunology Source Type: research
Stearoyl lysophosphatidylcholine enhances the phagocytic ability of macrophages through the AMP-activated protein kinase/p38 mitogen activated protein kinase pathway.
Abstract
A previous study showed that stearoyl lysophosphatidylcholine (sLPC) suppressed extracellular high mobility group box 1 translocation in macrophages stimulated with lipopolysaccharide through AMP-activated protein kinase (AMPK) activation. In the present study, we investigated whether sLPC-induced AMPK activation could enhance macrophages phagocytosis of bacteria. We found that sLPC increased phosphorylation of AMPK and acetyl-CoA carboxylase, a downstream target of AMPK, in a time- and dose-dependent manner in macrophages. Furthermore, sLPC increased the uptake of FITC-conjugated Escherichia coli by macr...
Source: International Immunopharmacology - August 8, 2016 Category: Allergy & Immunology Authors: Quan H, Hur YH, Xin C, Kim JM, Choi JI, Kim MY, Bae HB Tags: Int Immunopharmacol Source Type: research
Enhanced p62 Is Responsible for Mitochondrial Pathway-Dependent Apoptosis and Interleukin-1 β Production at the Early Phase by Monosodium Urate Crystals in Murine Macrophage
AbstractThe aim of this study was to clarify the role of p62-dependent mitochondrial apoptosis in the initiation of monosodium urate (MSU) crystal-induced inflammation in macrophages. The induction of mitochondrial apoptosis in RAW 264.7 murine macrophages by MSU crystals was measured using western blotting and quantitative real-time polymerase chain reaction for Bax, caspase-3, caspase-9, or PARP1, and by flow cytometric analysis. Immunoprecipitation and western blotting was applied to detect ubiquitination of p62, TRAF6, and caspase-9. Mitochondrial apoptosis, reactive oxygen species (ROS) generation, and cell proliferat...
Source: Inflammation - September 15, 2016 Category: Allergy & Immunology Source Type: research
Role of Leptin and SOCS3 in Inhibiting the Type I Interferon Response During Obesity
AbstractObesity provokes an imbalance in the immune system, including an aberrant type I interferon response during some viral infections and after TLR stimulation. SOCS3 overexpression and altered systemic leptin levels could be responsible for the reduced type I interferon production in people with obesity and, eventually, significantly increase the risk of viral infection. The aim of this study was to determine whether SOCS3- and leptin-induced tolerance are responsible for the reduced type I interferon production in people with obesity. SOCS3 overexpression in PBMCs from people with obesity was inhibited with the small...
Source: Inflammation - October 3, 2016 Category: Allergy & Immunology Source Type: research
The Fli-1 transcription factor is a critical regulator for controlling the expression of chemokine C-X-C motif ligand 2 (CXCL2)
In this study, we demonstrate that endothelial cells transfected with Fli-1 specific siRNA produce significantly less CXCL2 compared to cells transfected with control siRNA after stimulation by the Toll-like receptor (TLR) 4 ligands, lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNF-α). The production of CXCL2 in endothelial cells stimulated with LPS stimulation is dose-dependent. We found that Fli-1 binds to the CXCL2 promoter as established by Chromatin immunoprecipitation (ChIP) assay. Transient transfection assays show that Fli-1 drives transcription from the CXCL2 promoter in a dose-dependent manner an...
Source: Molecular Immunology - November 24, 2016 Category: Allergy & Immunology Source Type: research
AGEs Decreased SIRT3 Expression and SIRT3 Activation Protected AGEs-Induced EPCs ’ Dysfunction and Strengthened Anti-oxidant Capacity
AbstractAdvanced glycation end products (AGEs) have been confirmed to induce dysfunction in endothelial progenitor cells (EPCs) and play key roles in pathogenesis of diabetes-related vascular complications. The major function of sirtuin 3 (SIRT3) is to orchestrate oxidative metabolism and control reactive oxygen species (ROS) homeostasis, which are more closely related to EPCs ’ dysfunction. Our study therefore was designed to explore the role of SIRT3 on AGEs-induced EPCs dysfunction of. EPCs isolated from healthy adults were stimulated with AGEs and the expression of SIRT3 was assessed. Then, EPCs transfected with ad-S...
Source: Inflammation - December 28, 2016 Category: Allergy & Immunology Source Type: research
NLRP3 inflammasome activation regulated by NF- κB and DAPK contributed to paraquat-induced acute kidney injury
In conclusion, NLRP3 inflammasome activation regulated by NF-κB and DAPK played an important role in paraquat-induced acute kidne y injury.
Source: Immunologic Research - February 17, 2017 Category: Allergy & Immunology Source Type: research
