Metformin reduces autoimmune antibody levels in patients with Hashimoto's thyroiditis: A systematic review and meta-analysis.
CONCLUSION: Metformin significantly reduces TPOAb level and TgAb level in patients with HT and SH, especially TPOAb. This study is the first to provide a preliminary theoretical basis for the clinical application of metformin in the treatment of HT. PMID: 32741222 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - August 4, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

MicroRNA-340-5p suppressed rheumatoid arthritis synovial fibroblast proliferation and induces apoptotic cell number by targeting signal transducers and activators of transcription 3.
In this study, the role of microRNA-340-5p in rheumatoid arthritis was investigated. qRT-PCR was used to detect the expression of microRNA-340-5p in serums, synovial tissues, and fibroblast-like synoviocytes from patients and healthy participants. Cell proliferation rate, cell cycle and apoptotic cell numbers were measured by CCK-8 and flow cytometry assays. The expression of pro-inflammation factors was determined by ELISA. Our data showed that the expression of microRNA-340-5p was greatly suppressed in rheumatoid arthritis serums, synovial tissues and rheumatoid arthritis-fibroblast-like synoviocytes compared to that in ...
Source: Autoimmunity - July 28, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Expression of NGAL-specific cells and mRNA levels correlate with inflammation in the salivary gland, and its overexpression in the saliva, of patients with primary Sj ögren's syndrome.
Expression of NGAL-specific cells and mRNA levels correlate with inflammation in the salivary gland, and its overexpression in the saliva, of patients with primary Sjögren's syndrome. Autoimmunity. 2020 Jul 20;:1-11 Authors: Aqrawi LA, Jensen JL, Fromreide S, Galtung HK, Skarstein K Abstract Salivary gland involvement is a characteristic feature of primary Sjögren's syndrome (pSS), where tissue destruction is mediated by infiltrating immune cells, and may be accompanied by the presence of adipose tissue. Optimally diagnosing this multifactorial disease requires the incorporation of additional...
Source: Autoimmunity - July 22, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Autoantibodies to intracellular "rods and rings" structures in two patients with autoimmune hepatitis treated with azathioprine.
Autoantibodies to intracellular "rods and rings" structures in two patients with autoimmune hepatitis treated with azathioprine. Autoimmunity. 2020 Jul 14;:1-4 Authors: Galaski J, Koop AC, Lütgehetmann M, Lohse AW, Weiler-Normann C Abstract In vitro, inhibition of the synthesis of guanosine monophosphate (GMP) by various drugs such as ribavirin, acyclovir, azathioprine, and mycophenolic acid leads to the formation of subcellular structures in cultured cells. Autoantibodies targeting these cellular structures can be detected as "rods and rings" (RR) patterns by immunofluorescenc...
Source: Autoimmunity - July 15, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Identification of differentially expressed and methylated genes associated with rheumatoid arthritis based on network.
This study integrated three expression profile datasets (GSE55235, GSE12021, and GSE55457) and one methylation profile dataset GSE111942 to elucidate the potential essential candidate genes and pathways in RA. Differentially expressed genes (DEGs) and differentially methylation genes (DMGs) were identified by R programming software, using Limma package and ChAMP package, respectively. DAVID performed gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs. Functional annotation and construction of a protein-protein interaction (PPI) network and the Molecular Complex Detect...
Source: Autoimmunity - July 12, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Whole-exome sequencing in three children with sporadic Blau syndrome, one of them co-presenting with recurrent polyserositis.
We report on three Mexican patients clinically diagnosed with BS who exhibited a likely pathogenic variant in NOD2 as revealed by whole-exome sequencing (WES) and Sanger sequencing: two variants (c.1000 C > T/p.Arg334Trp and c.1538 T > C/p.Met513Thr) lie in the ATP/Mg2+ binding site, whereas the other (c.3019dupC/p.Leu1007ProfsTer2) introduces a premature stop codon disrupting the last LRR domain (LRR9) formation; all three variants are consistent with gain-of-function changes. Interestingly, all these patients presented concomitant likely pathogenic variants in other inflammatory disease-...
Source: Autoimmunity - July 2, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Enhanced expression of human endogenous retroviruses in new-onset type 1 diabetes: Potential pathogenetic and therapeutic implications.
Authors: Tovo PA, Rabbone I, Tinti D, Galliano I, Trada M, Daprà V, Cerutti F, Bergallo M Abstract Human endogenous retroviruses (HERVs) have been studied and proposed as relevant cofactors in several autoimmune diseases, including type 1 diabetes (T1D), though with controversial results and no study at disease onset. In order to gather further information on the potential role of HERVs in the development of T1D we assessed the transcription levels of pol genes of HERV-H, HERV-K, and HERV-W in peripheral leucocytes from 37 children and adolescents with new-onset T1D and 50 age-matched control subjects. A PCR...
Source: Autoimmunity - June 28, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Feeding lactobacilli impacts lupus progression in (NZBxNZW)F1 lupus-prone mice by enhancing immunoregulation.
Authors: Manirarora JN, Kosiewicz MM, Alard P Abstract Although the relationship between autoimmunity and microorganisms is complex, there is evidence that microorganisms can prevent the development of various autoimmune diseases. Lactobacilli are beneficial gut bacteria that play an important role in immune system development. The goals of this study were to assess the ability of three different strains of lactobacilli (L. casei B255, L. reuteri DSM 17509 and L. plantarum LP299v) to control lupus development/progression in (NZBxNZW)F1 (BWF1) lupus-prone mice before and after disease onset, and identify the mechani...
Source: Autoimmunity - June 21, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

CXCL4 promoted the production of CD4+CD25+FOXP3+treg cells in mouse sepsis model through regulating STAT5/FOXP3 pathway.
Authors: Xu T, Zhao J, Wang X, Meng Y, Zhao Z, Bao R, Deng X, Bian J, Yang T Abstract Background: CXCL4 plays an essential role in the regulation of multiple immune diseases. However, the underlying role of CXCL4 is still not clear in sepsis. Aim: In the present study, we aimed to investigate the function of CXCL4 in sepsis.Methods: Sepsis model was constructed on mouse. Flow cytometry was used to determine the ratio of CD4+CD25+FOXP3+Treg cells. ELISA assays were used to determine the levels of CXCL4, IL-6, IL-10, and TNF-α respectively. Western blot was used to examine protein contents.Results: Our results ...
Source: Autoimmunity - June 18, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

LncRNA PlncRNA-1 participates in rheumatoid arthritis by regulating transforming growth factor β1.
LncRNA PlncRNA-1 participates in rheumatoid arthritis by regulating transforming growth factor β1. Autoimmunity. 2020 Jun 16;:1-6 Authors: Wang Y, Li G, Guo W, Ji B, Aisikeerbayi A, Boratibai Y, Cao L Abstract LncRNA PlncRNA-1(PlncRNA-1) participates in breast cancer by upregulating TGF-β1. It is known that TGF-β1 plays an inhibitory role in the inflammatory responses in rheumatoid arthritis (RA). Therefore, PlncRNA-1 may also participate in RA. Serum and synovial fibroblasts were obtained from 34 patients with active RA (persistent symptoms), 36 patients with inactive RA (long term of n...
Source: Autoimmunity - June 18, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Identification of antigen-specific neutrophils in the tonsils with recurrent acute inflammation.
This study aims to identify the antigen-specific PMNs (sPMNs) isolated from the tonsillar tissues with recurrent acute inflammation. In this study, CD66b+ PMNs were isolated from surgically removed tonsils (40 tonsils were from 20 Rtn patients; 24 tonsils were from 12 tonsil tumour patients) by flow cytometry cell sorting. sPMNs were identified through immunological approaches. We found that compared with the control tonsil samples (from marginal non-tumour tissues of tonsil cancer), Rtn samples showed higher PMN frequency, higher levels of myeloperoxidase (MPO) and neutrophil elastase (NE), in which positive correlation w...
Source: Autoimmunity - June 12, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Correction.
Authors: PMID: 32450721 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - May 28, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Decrease in alpha-1 antiproteinase antitrypsin is observed in primary Sjogren's syndrome condition.
Authors: Singh BB, Ohm J, Quenum Zanbede FO, Chauhan P, Kroese FGM, Vissink A, Ambrus JL, Mishra BB Abstract Primary Sjogren's syndrome (pSS) is a systemic autoimmune disease that is characterized by the infiltration of immune cells. Although the loss of salivary gland function is a major manifestation observed in pSS, the factors that could promote these changes in salivary gland tissue in pSS is not yet determined. Herein, we provide evidence that loss of alpha-1 antiproteinase antitrypsin could contribute to the induction of pSS. Alpha-1 antiproteinase antitrypsin belongs to the family of serpin proteins that fu...
Source: Autoimmunity - May 26, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Soluble PD1 levels are increased with disease activity in paediatric onset autoimmune hepatitis and inflammatory bowel disease.
Authors: Hadley T, Gillespie S, Espinoza H, Prince J, Gronbaek H, Chandrakasan S, Kuguthasan S, Kolachala VL, Gupta NA Abstract Introduction: Immune mediated liver diseases entail a broad category which are associated with increased morbidity and mortality amongst the paediatric population. Programmed Death 1 (PD1) is an inhibitory receptor mainly expressed by T cells, and when activated shed into plasma as soluble PD1(sPD1). The AIM of this study was to evaluate sPD1 levels in plasma of paediatric patients with Autoimmune Hepatitis (AIH), Primary Sclerosing Cholangitis (PSC), AIH and PSC overlap, Inflammatory Bowe...
Source: Autoimmunity - May 8, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Autoantibodies against the autophagic protein microtubule-associated light-chain 3 (LC3): Immunocharacterization of an atypical ANA pattern.
Authors: Maellaro E, Terzuoli L, Bacarelli MR, Del Bello B, Bizzaro N, Porcelli B Abstract Autoantibodies to nuclear and cytoplasmic antigens are commonly detected by indirect immunofluorescence (IIF) on HEp-2 cells, and three major staining patterns (nuclear, cytoplasmic, and mitotic) are distinguished. Here, we report an atypical cytoplasmic pattern, not described so far, observed in the serum of a patient with a controversial diagnosis of systemic lupus erythematosus (SLE). Moreover, for the first time, we have revealed the presence of autoantibodies against the microtubule-associated light-chain 3 (LC3) protein...
Source: Autoimmunity - April 29, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Simultaneous measurement of twenty-nine circulating cytokines and growth factors in female patients with overt autoimmune thyroid diseases.
In conclusion, BAFF is the best circulating indicator to identify GD and HT among all chosen 29 biomarkers, and it could be used to predict the disease severity in HT and active GD. Meanwhile, IFN-α could be another reliable parameter for recognising HT. PMID: 32338082 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - April 29, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

MiR-153-3p induces immune dysregulation by inhibiting PELI1 expression in umbilical cord-derived mesenchymal stem cells in patients with systemic lupus erythematosus.
Authors: Li D, Li X, Duan M, Dou Y, Feng Y, Nan N, Zhang W Abstract Mesenchymal stem cells (MSCs) are identified as a promising tool for the treatment of autoimmune diseases, and several microRNAs (miRNAs) are shown to exhibit vital roles in immune diseases. However, their function and mechanism in systemic lupus erythematosus (SLE) is still unclear. The qRT-PCR analysis was employed to investigate level of miR-153-3p. Subsequently, western blot and luciferase reporter assays were carried out to determine miR-153-3p targets. Cell proliferation and migration were determined using EdU proliferation assays and transwe...
Source: Autoimmunity - April 25, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Retroviruses in the pathogenesis of systemic lupus erythematosus: Are they potential therapeutic targets?
Authors: Talotta R, Atzeni F, Laska MJ Abstract The pathogenesis of systemic lupus erythematosus (SLE) is characterised by the hyper-activation of immunologic pathways related to the antiviral response. Exogenous and endogenous retroviruses, by integrating their DNA templates in the host cell genome, may epigenetically control the transcription of genes involved in the immune response. Furthermore, their nucleic acids or neo-synthesized proteins could stimulate the sensor molecules placed upstream the inflammatory cascade. Exogenous retroviruses, like human immunodeficiency virus, have been associated to SLE-like m...
Source: Autoimmunity - April 25, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Nanomedicine-mediated prevention of inflammatory monocytes infiltration ameliorate ovalbumin-induced allergic rhinitis in mouse model.
Authors: Gu X, Xiao F, Lu W, Xu Y, Li X, Yu C, Cui X Abstract Th2 immune cells infiltration into nasal mucosa is one of the characters of allergic rhinitis (AR). We aimed to explore whether inhibition of Th2 immune cells infiltration would attenuate AR progression. AR mouse model was established by i.p. injection of ovalbumin (OVA). The infiltrated immune cells into nasal lavage fluid were detected by flow cytometry. Cytokine concentration in serum was determined by ELISA. AR mice symptoms were indicated by the number of sneezing and nasal rubbing events. In AR mice, CCL2 expression levels and CD45+CD11b+Ly6Chi inf...
Source: Autoimmunity - April 16, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Assessing vitamin D levels in an anti-DFS70 positive population: New insights emerging.
Conclusions: Our findings showed higher levels of vitamin D in anti-DFS70 positive subjects than in the controls, which is compatible with the hypothesis of the "benign" nature of anti-DFS70 antibodies. PMID: 32164458 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - March 14, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Abnormal expression of BAFF and its receptors in peripheral blood and skin lesions from systemic lupus erythematosus patients.
Authors: Chen Y, Yang M, Long D, Li Q, Zhao M, Wu H, Lu Q Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease that is characterized by abnormal T and B cells. B-cell activating factor (BAFF) has been suggested to play a crucial role in lupus by promoting the proliferation, differentiation, and survival of B cells. Increased serum levels of BAFF have been found in patients with lupus. However, the expression of BAFF and its receptors on immune cells and in skin has not been systematically reported before. Here, we report that SLE patients showed increased levels of BAFF on circulating CD3+ T cells a...
Source: Autoimmunity - March 13, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Serum IL-21 levels from celiac disease patients correlates with anti-tTG IgA autoantibodies and mucosal damage.
Conclusions: A role of gluten, as antigen with stimulatory function on IL-21 production, seems to be confirmed by the longitudinal analyses showing that the gluten-free diet decreases to a nearly undetectable amount this cytokine. In addition, the finding of a positive correlation between the serum amount of IL-21 and the grade of duodenal mucosa damage suggests a strong immunomodulatory effect of this cytokine on cytotoxic T lymphocyte functions. This study provides an extra evidence to emerging data on the potential role IL-21 in CD pathogenesis, suggesting its involvement in the development and progression of CD. Signif...
Source: Autoimmunity - March 13, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

CMA1 is potent prognostic marker and associates with immune infiltration in gastric cancer.
Conclusion: CMA1 is a key gene whose expression level is significantly correlated with GC prognosis and infiltration levels of CD8+, CD4+ T cells, neutrophils, macrophages, and DCs in GC. In addition, the expression of CMA1 may be involved in regulating tumour-associated macrophages (TAMs), dendritic cells, exhausted T cells and regulatory T cells in GC. It suggests that CMA1 could be utilized as a prognostic marker and a sign of immune infiltration in GC. PMID: 32129682 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - March 6, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Circular RNAs hsa_circ_0000479 in peripheral blood mononuclear cells as novel biomarkers for systemic lupus erythematosus.
In this study, 11 circRNAs (hsa_circ_0000479, hsa_circ_0002316, hsa_circ_0000317, hsa_circ_0082688, hsa_circ_0082689, hsa_circ_0087798, hsa_circ_0008529, hsa_circ_0000787, hsa_circ_0021727, hsa_circ_0000175, and hsa_circ_0003694) which were found to be significantly up-regulated in both peripheral blood mononuclear cells (PBMCs) from SLE patients in our previous study, and T cells from SLE patients in previous literature, were chosen for validation by quantitative reverse transcription-polymerase chain reaction in PBMCs from 50 new-onset SLE patients, 24 new-onset rheumatoid arthritis (RA) patients, 24 new-onset ankylosing...
Source: Autoimmunity - February 26, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Identification of key biomolecules in rheumatoid arthritis through the reconstruction of comprehensive disease-specific biological networks.
In this study, several gene expression data including synovial tissue and macrophages from synovial tissues were integrated with a holistic perspective and the molecular targets and signatures in RA were determined. Differentially expressed genes (DEGs) were identified from each dataset by comparing diseased and healthy samples. Afterward, the RA-specific protein-protein interaction (PPI) and the transcriptional regulatory network were reconstructed by using several biomolecule interaction data. Key biomolecules were determined through a statistical test employing the hypergeometric probability density function by using th...
Source: Autoimmunity - February 6, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

DNA methylation changes on immune cells in Systemic Lupus Erythematosus.
Authors: Hurtado C, Acevedo Sáenz LY, Vásquez Trespalacios EM, Urrego R, Jenks S, Sanz I, Vásquez G Abstract DNA methylation as a process that regulates gene expression is crucial in immune cells biology. Global and gene specific methylation changes have been described in autoimmunity, especially in Systemic Lupus Erythematosus. These changes not only contribute to the understanding of the disease, but also some have been proposed as diagnostic or disease activity biomarkers. The present review compiles the most recent discoveries on this field on each type of immune cells, including specific c...
Source: Autoimmunity - February 6, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

IL1 β, IL18, NFKB1 and IFNG gene interactions are associated with severity of rheumatoid arthritis: A pilot study.
IL1β, IL18, NFKB1 and IFNG gene interactions are associated with severity of rheumatoid arthritis: A pilot study. Autoimmunity. 2020 Jan 29;:1-7 Authors: Gomes da Silva IIF, Lima CAD, Monteiro MLA, Barboza DASP, Rushansky E, Mariano MHQA, Sandrin-Garcia P, de Souza PRE, Maia MMD Abstract Rheumatoid arthritis (RA) is an autoimmune disease which can lead to progressive and functional disability. Literature data suggest that some inflammatory proteins are dysregulated in RA patients and its genetic polymorphisms may contribute to the aetiology and pathogenesis of disease in different ethnic groups. P...
Source: Autoimmunity - January 30, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Annexin-coated particles induce antigen-specific immunosuppression.
Authors: Link C, Bujupi F, Krammer PH, Weyd H Abstract Apoptotic cells mediate the development of tolerogenic dendritic cells (DC) and thus facilitate induction and maintenance of peripheral tolerance. Following the identification of the evolutionary conserved annexin core domain (Anx) as a specific signal on apoptotic cells which antagonises Toll-like receptor (TLR) signalling, we examined whether the tolerogenic capacity of Anx can be exploited to downregulate antigen-specific immune responses. The treatment of bone marrow-derived dendritic cells (BMDC) with particles harbouring Anx as well as the model antigen o...
Source: Autoimmunity - January 16, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Human antimicrobial peptides in autoimmunity.
Authors: Umnyakova ES, Zharkova MS, Berlov MN, Shamova OV, Kokryakov VN Abstract Antimicrobial peptides (AMPs) were firstly discovered as cytotoxic substances that killed bacteria. Later they were described as biologically active peptides that are able not only to kill invaders but also to modulate host immunity. In particular, it is shown that human antimicrobial peptides are able to influence the activity of different innate and adaptive immunity components, thus, obviously, they also participate in autoimmune processes. In this review we discuss the nature of human AMPs and analyze their role in such autoimmune ...
Source: Autoimmunity - January 10, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Identifying the differentially expressed microRNAs in autoimmunity: A systemic review and meta-analysis.
Authors: Zhang L, Wu H, Zhao M, Lu Q Abstract The evidence indicates that microRNAs (miRNAs) can regulate gene expression and play an important role in the pathogenesis of autoimmune diseases, yet studies on expression profiles of miRNAs are still inconclusive. Our objective is to identify miRNAs that demonstrate enduring differential expression on autoimmune diseases. A systemic review and meta-analysis were performed by analysing the expression profile of miRNAs in several types of autoimmune disease, including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and type-1 diabetes (T1D). Several most ...
Source: Autoimmunity - January 7, 2020 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

The pathological role of B cells in systemic lupus erythematosus: From basic research to clinical.
Authors: Feng Y, Yang M, Wu H, Lu Q Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease that often occurs in females of child-bearing age. It involves multiple systems and severely threatens human life. One of the typical characteristics of SLE is the formation of immune complexes with autoantibodies produced by B cells that target various autoantigens, thus indicating the pivotal role of B cells in the pathogenesis of SLE. Increasing evidence has shown abnormal expression of B cells in the peripheral blood of SLE patients. Moreover, numerous studies have shown that B cells in SLE patients are abno...
Source: Autoimmunity - December 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis.
Authors: Cavalcanti CAJ, Germoglio V, de Azevêdo Silva J, Glesse N, Vianna P, Cechim G, Monticielo OA, Xavier RM, Brenol JCT, Brenol CV, Fragoso TS, Barbosa AD, Duarte ÂLBP, Oliveira RDR, Louzada-Júnior P, Donadi EA, Chies JAB, Crovella S, Sandrin-Garcia P Abstract The aim of the present study was to evaluate the impact of SEMA4A genetic variants on expression of sema4A protein and its relation to autoimmunity development in Systemic Lupus Erythematosus and Rheumatoid Arthritis patients. A total of 541 SLE patients, 390 RA patients and 607 healthy individuals were genotyped. We also assessed SEMA...
Source: Autoimmunity - December 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

A novel 4-dimensional live-cell imaging system to study leukocyte-endothelial dynamics in ANCA-associated vasculitis.
This study aimed to develop a 4-dimensional (4D) live-cell imaging system that would enable investigation of spatial and temporal dynamics of these cells in health and disease. We further aimed to validate this system using autologous donor serum from AAV patients and polyclonal ANCA IgG, as well as exploring its potential in the pre-clinical testing of putative therapeutic compounds. Neutrophils and monocytes were isolated from peripheral venous blood of AAV patients or healthy controls and co-incubated on an endothelial monolayer in the presence of autologous serum. Alternatively, polyclonal ANCA IgG was used, following ...
Source: Autoimmunity - December 24, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

The -675 4G/5G PAI-1 polymorphism confers genetic susceptibility to systemic lupus erythematosus, its clinical manifestations, and comorbidities in Mexican-Mestizo population.
Authors: Anaya-Macias BU, De la Cruz-Mosso U, Palafox-Sánchez CA, Parra-Rojas I, Martínez-Bonilla G, González-López L, Gámez-Nava JI, Pérez-Guerrero EE, Barrientos-Avalos SL, Muñoz-Valle JF Abstract Systemic lupus erythematosus (SLE) involves a broad range of factors that contribute to the development of the disease and its comorbidities. Genetic predisposition influences the development of SLE, and the -675 4G/5G PAI-1 polymorphism has been associated with several pathologies with a chronic inflammatory component. Our objective was to investigate the genetic associa...
Source: Autoimmunity - December 14, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Low frequency of IL-10-producing B cells and high density of ILC2s contribute to the pathological process in Graves' disease, which may be related to elevated-TRAb levels.
In this study, we analyzed the frequencies of B10, Treg cells and ILC2s, as well as the expression of related cytokines in peripheral blood from patients with Graves' disease and evaluated the correlation between B10 cell numbers and autoantibodies level. Our data showed that the frequency of B10 or Treg cells was significantly decreased in peripheral blood mononuclear cells from Graves' disease patients, while the percentage of ILC2s cells was increased; the levels of cytokine IL-5, IL-13 and related transcription factor RORα were up-regulated. Autoantibodies analysis also showed that high level of TRAb was accompan...
Source: Autoimmunity - December 7, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Deciphering crucial genes in coeliac disease by bioinformatics analysis.
Authors: Noori E, Kazemi B, Bandehpour M, Zali H, Khalesi B, Khalili S Abstract Coeliac disease (CD) is a chronic autoimmune disease that is characterized by malabsorption in sensitive individuals. CD is triggered by the ingestion of grains containing gluten. CD is concomitant with several other disorders, including dermatitis herpetiformis, selective IgA deficiency, thyroid disorders, diabetes mellitus, various connective tissue disorders, inflammatory bowel disease, and rheumatoid arthritis. The advent of high throughput technologies has provided a massive wealth of data which are processed in various omics scale...
Source: Autoimmunity - December 7, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Effect of high mobility group box 1 on Toll-like receptor 9 in B cells in myeloperoxidase-ANCA-associated vasculitis.
Authors: Wang C, Deng H, Gong Y, You R, Chen M, Zhao MH Abstract High mobility group box 1 (HMGB1) played pathogenic role in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Recent findings demonstrated that Toll-like receptor 9 (TLR9) was involved in B cell tolerance breaking of autoimmune disease, including AAV. Here, we investigated the effect of HMGB1 on TLR9 in B cells of AAV. In the present work, patients with myeloperoxidase (MPO)-AAV in active stage were recruited. Intracellular TLR9 expression in various B cell subpopulations of the whole blood was detected by flow cytometry and the ...
Source: Autoimmunity - December 4, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Lower proportion of CD19+IL-10+ and CD19+CD24+CD27+ but not CD1d+CD5+CD19+CD24+CD27+ IL-10+ B cells in children with autoimmune thyroid diseases.
Conclusions: Our data demonstrate that the reduction in the number of Bregs with CD19+CD24+CD27+IL-10+ and CD19+IL-10+ expression could be responsible for breaking immune tolerance and for AITD development in children. PMID: 31790305 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - December 4, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance.
In this study, we utilize female SD rats induced HT to evaluated the ability of transplanted MSCs to regulate Th17/Treg interactions in a rat Hashimoto's thyroiditis (HT) model system. The results showed that Rats in the HT model group exhibited increased thyroid autoantibody levels consistent with successful model development, whereas these levels were lower in rats treated with MSCs. There were also fewer thyroid lesions and less lymphoid infiltration of the thyroid in MSC-treated rats relative to HT model rats, as well as fewer Th17 cells and more Treg cells - an observation consistent with the cytokine analyses. All of...
Source: Autoimmunity - December 4, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Diagnostic performance of aPS/PT antibodies in neuropsychiatric lupus and cardiovascular complications of SLE.
Conclusion: aPS/PT antibodies provide non-redundant information that could contribute to risk assessment and stratification of patients with SLE. PMID: 31782311 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - December 1, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Th1, Th2, and Th17 cytokines in systemic lupus erythematosus.
Authors: Muhammad Yusoff F, Wong KK, Mohd Redzwan N Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the breakdown of immune tolerance leading to excessive inflammation and tissue damage. Imbalance in the levels of cytokines represents one of the multifactorial causes of SLE pathogenesis and it contributes to disease severity. Deregulated levels of T helper type 1 (Th1), type 2 (Th2), and type 17 (Th17) cytokines have been associated with autoimmune inflammation. Growing evidence has shown deregulated levels of Th1, Th2, and Th17 cytokines in SLE patients compared to healthy con...
Source: Autoimmunity - November 29, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Association study between X-linked susceptibility genes and clinical features in Chinese female patients with systemic lupus erythematosus.
Authors: Li S, Di D, Wu X, Zhang L, Liu R, Huang Q, Pan H, Ye D, Leng R Abstract Recent large-scale genetic association studies have identified that several single nucleotide polymorphisms (SNPs) on X chromosome are correlated with risk of systemic lupus erythematosus (SLE) in Chinese population. The aim of this study was to estimate association between these loci and clinical features in female patients with SLE. Six SNPs identified in previous studies were genotyped. Odds ratio (OR) was calculated with adjusting for potential confounding factors. A total of 772 SLE patients were included in the final analysis. Th...
Source: Autoimmunity - November 13, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Autoantigen-specific B cells and plasma cells are prominent in areas of fatty infiltration in salivary glands of patients with primary Sj ögren's syndrome.
Autoantigen-specific B cells and plasma cells are prominent in areas of fatty infiltration in salivary glands of patients with primary Sjögren's syndrome. Autoimmunity. 2019 Oct 30;:1-9 Authors: Skarstein K, Jensen JL, Galtung H, Jonsson R, Brokstad K, Aqrawi LA Abstract Salivary and lacrimal gland involvement is a characteristic feature of primary Sjögren's syndrome (pSS), where tissue destruction is mediated by mononuclear cell infiltration, resulting in lacrimal and salivary gland impairment. We have previously shown distinct prevalence of adipose tissue replacement in the minor salivary g...
Source: Autoimmunity - October 31, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Bisphenol A modified DNA: A possible immunogenic stimulus for anti-DNA autoantibodies in systemic lupus erythematosus.
This study was undertaken to investigate the contribution of BPA induced DNA damage in SLE patients. Human DNA was modified by BPA in-vitro and the binding characteristics of SLE circulating immunoglobulin Gs (SLE-IgGs) with BPA damaged DNA (BPA-DNA) were screened and compared with the IgGs from normal healthy humans (NH-IgGs). Immunogenicity of BPA-DNA was determined by immunisation in rabbits. DNA from SLE patients (SLE-DNA) or healthy humans (NH-DNA) were isolated and their binding specificity with rabbit anti-BPA-DNA-IgGs was studied. Treatment of human DNA with BPA caused extensive damaged. Circulating SLE-IgGs showed...
Source: Autoimmunity - October 30, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Gene-gene interaction and association of Wnt/ & Beta;-catenin signalling pathway gene polymorphisms with ankylosing spondylitis susceptibility in the Chinese Han population.
Conclusions: Our research implies a potential gene-gene interaction, thus revealing the importance of the Wnt/β-catenin signalling pathway for understanding the genetic architecture of AS. PMID: 31656088 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - October 30, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Diagnostic value of 18-F fluorodeoxyglucose PET/CT and bone scan in Schnitzler syndrome.
Conclusion: Bone scans may be favoured over PET/CT in Schnitzler syndrome. PMID: 31646899 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - October 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Long non coding RNA H19: An emerging therapeutic target in fibrosing diseases.
Authors: Li J, Cao LT, Liu HH, Yin XD, Wang J Abstract Fibrosis is characterised by excessive deposition of the extracellular matrix (ECM) and develops because of fibroblast differentiation during the process of inflammation. There are few effective treatment options for this diseases due to the aetiology of fibrosis is not completely clarified. Long non-coding RNAs (lncRNAs), a type of ncRNA with a length of greater than 200 nucleotides without evident protein coding function, are important regulators of most biological and pathological processes, including participation, regulation or mediation of disease develop...
Source: Autoimmunity - October 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

COX-2 induces T cell accumulation and IFN- γ production during the development of chromium allergy.
In this study, mice were used with Cr-induced allergy challenge model. The mice were injected with celecoxib once per day for 7 days one hour after the challenge. Footpad samples were stained with haematoxylin and eosin (H&E), and lymphocytes were isolated from popliteal lymph nodes for the flow cytometric analysis. The results show that both prostaglandin E2 (PGE2), a known mediator of inflammation, and cyclooxygenases (COX)-2 have important roles in the development of Cr allergy. Further, COX-2 inhibitor, celecoxib, was effective in relieving swelling and inflammation in Cr-allergic mice concordant with suppression o...
Source: Autoimmunity - October 8, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Blau syndrome with a rare mutation in exon 9 of NOD2 gene.
We reported a 41-year old female Turkish patient diagnosed as Blau syndrome. Granulomatous dermatitis and severe headache, as well as recurrent chest and pelvic pain have been present since she was 8 years old. Arthritis started when she was teenage, hypertension diagnosed when she was 20 and other symptoms also occurred during the lifetime (severe preeclampsia, ischemic stroke, recurrent hemiparesis, recurrent-transient-vision-loss and renal-artery-stenosis). Genomic DNA was isolated from peripheral blood and 12 genes sequenced in Autoinflammatory panel on IonTorrent-S5-NGS platform with Parseq-VariFind™AIPas...
Source: Autoimmunity - September 28, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Association of IL6 gene methylation in peripheral blood cells with the development and prognosis of autoimmune thyroid diseases.
In conclusion, IL6 gene methylation levels were related to the susceptibility to HD and the intractability of GD. PMID: 31552761 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - September 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research