Autoantigen-specific B cells and plasma cells are prominent in areas of fatty infiltration in salivary glands of patients with primary Sj ögren's syndrome.
Autoantigen-specific B cells and plasma cells are prominent in areas of fatty infiltration in salivary glands of patients with primary Sjögren's syndrome. Autoimmunity. 2019 Oct 30;:1-9 Authors: Skarstein K, Jensen JL, Galtung H, Jonsson R, Brokstad K, Aqrawi LA Abstract Salivary and lacrimal gland involvement is a characteristic feature of primary Sjögren's syndrome (pSS), where tissue destruction is mediated by mononuclear cell infiltration, resulting in lacrimal and salivary gland impairment. We have previously shown distinct prevalence of adipose tissue replacement in the minor salivary g...
Source: Autoimmunity - October 31, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Bisphenol A modified DNA: A possible immunogenic stimulus for anti-DNA autoantibodies in systemic lupus erythematosus.
This study was undertaken to investigate the contribution of BPA induced DNA damage in SLE patients. Human DNA was modified by BPA in-vitro and the binding characteristics of SLE circulating immunoglobulin Gs (SLE-IgGs) with BPA damaged DNA (BPA-DNA) were screened and compared with the IgGs from normal healthy humans (NH-IgGs). Immunogenicity of BPA-DNA was determined by immunisation in rabbits. DNA from SLE patients (SLE-DNA) or healthy humans (NH-DNA) were isolated and their binding specificity with rabbit anti-BPA-DNA-IgGs was studied. Treatment of human DNA with BPA caused extensive damaged. Circulating SLE-IgGs showed...
Source: Autoimmunity - October 30, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Gene-gene interaction and association of Wnt/ & Beta;-catenin signalling pathway gene polymorphisms with ankylosing spondylitis susceptibility in the Chinese Han population.
Conclusions: Our research implies a potential gene-gene interaction, thus revealing the importance of the Wnt/β-catenin signalling pathway for understanding the genetic architecture of AS. PMID: 31656088 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - October 30, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Diagnostic value of 18-F fluorodeoxyglucose PET/CT and bone scan in Schnitzler syndrome.
Conclusion: Bone scans may be favoured over PET/CT in Schnitzler syndrome. PMID: 31646899 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - October 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Long non coding RNA H19: An emerging therapeutic target in fibrosing diseases.
Authors: Li J, Cao LT, Liu HH, Yin XD, Wang J Abstract Fibrosis is characterised by excessive deposition of the extracellular matrix (ECM) and develops because of fibroblast differentiation during the process of inflammation. There are few effective treatment options for this diseases due to the aetiology of fibrosis is not completely clarified. Long non-coding RNAs (lncRNAs), a type of ncRNA with a length of greater than 200 nucleotides without evident protein coding function, are important regulators of most biological and pathological processes, including participation, regulation or mediation of disease develop...
Source: Autoimmunity - October 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

COX-2 induces T cell accumulation and IFN- γ production during the development of chromium allergy.
In this study, mice were used with Cr-induced allergy challenge model. The mice were injected with celecoxib once per day for 7 days one hour after the challenge. Footpad samples were stained with haematoxylin and eosin (H&E), and lymphocytes were isolated from popliteal lymph nodes for the flow cytometric analysis. The results show that both prostaglandin E2 (PGE2), a known mediator of inflammation, and cyclooxygenases (COX)-2 have important roles in the development of Cr allergy. Further, COX-2 inhibitor, celecoxib, was effective in relieving swelling and inflammation in Cr-allergic mice concordant with suppression o...
Source: Autoimmunity - October 8, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Blau syndrome with a rare mutation in exon 9 of NOD2 gene.
We reported a 41-year old female Turkish patient diagnosed as Blau syndrome. Granulomatous dermatitis and severe headache, as well as recurrent chest and pelvic pain have been present since she was 8 years old. Arthritis started when she was teenage, hypertension diagnosed when she was 20 and other symptoms also occurred during the lifetime (severe preeclampsia, ischemic stroke, recurrent hemiparesis, recurrent-transient-vision-loss and renal-artery-stenosis). Genomic DNA was isolated from peripheral blood and 12 genes sequenced in Autoinflammatory panel on IonTorrent-S5-NGS platform with Parseq-VariFind™AIPas...
Source: Autoimmunity - September 28, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Association of IL6 gene methylation in peripheral blood cells with the development and prognosis of autoimmune thyroid diseases.
In conclusion, IL6 gene methylation levels were related to the susceptibility to HD and the intractability of GD. PMID: 31552761 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - September 27, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

IP-10 and MCP-1 gene polymorphisms in Chinese patients with chronic immune thrombocytopenia.
In conclusion, the -201 G/A polymorphism of IP-10 gene may be associated with the susceptibility of ITP in Chinese population. PMID: 31530029 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - September 20, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Vasculitis and crescentic glomerulonephritis in a newly established congenic mouse strain derived from ANCA-associated vasculitis-prone SCG/Kj mice.
Authors: Hamano Y, Ito F, Suzuki O, Koura M, Matsuoka S, Kobayashi T, Sugitani Y, Wali N, Koyanagi A, Hino O, Suzuki S, Sugamata R, Yoshizawa H, Yumura W, Maruyama N, Kameoka Y, Noda Y, Hasegawa Y, Arai T, Suzuki K Abstract Lupus nephritis (LN) is the secondary glomerulonephritis (GN) involved in systemic lupus erythematosus (SLE) and a typical immune complex-type GN. Antineutrophil cytoplasmic autoantibody (ANCA)-associated vasculitis (AAV) is an autoimmune disease characterized by systemic vasculitis and pauci-immune-type crescentic glomerulonephritis (CrGN) with ANCA production. Human AAV causes death due to lun...
Source: Autoimmunity - September 4, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

The gene mutations and subtelomeric DNA methylation in immunodeficiency, centromeric instability and facial anomalies syndrome.
Authors: Hu H, Chen C, Shi S, Li B, Duan S Abstract Immunodeficiency, centromeric instability and facial anomalies syndrome (ICF) is a rare autosomal recessive disorder, which is characteristic of a severe impairment of immunity. In the genetic aspect, ICF is featured with mutations primarily located in the specific genes (DNMT3B for ICF1, ZBTB24 for ICF2, CDCA7 for ICF3, and HELLS for ICF4). The subtelomeric region is defined as 500 kb at the terminal of each autosomal arm. And subtelomeric DNA fragments can partially regulate key biological activities, including chromosome movement and localization in the ...
Source: Autoimmunity - September 4, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Impacts of GFP-FoxP3+ regulatory T cells on lupus hallmarks differ by genetic background and type of GFP knock-in.
Authors: Chang SH, Kim TJ, Kim Y, Han SS, Lee SK, Sim JH, Kim YJ, Lee SJ, Rhyu IJ, Nam KH, Mohan C, Kim HR Abstract FoxP3 reporter mice expressing green fluorescence protein (GFP) have been used as a very convenient tool to investigate the impact of regulatory T (Treg) cells on pathogenesis in autoimmune diseases. Here, we found that GFP-FoxP3+ knock-in (KI) mice showed alterations in the production of anti-nuclear autoantibodies (ANAs) and nephritis with different extent, depending on the presence or absence of lupus susceptibility gene locus 1 (Sle1) and KI method: contrasting with B6.Sle1.fGFP-FoxP3 mice, expres...
Source: Autoimmunity - September 1, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models.
This study investigated the influence of autophagy on the expression of Collagen type II and light chain 3 (LC-3) in the articular cartilage of osteoarthritis (OA) models. The expression of OA associated biomarkers namely Matrix metalloproteinase (MMP-13), NOD-, LRR- and pyrin domain-containing 3 (NLRP3) induced by destabilizing the medial meniscus operation (DMM) were also investigated. A total of 60 C57BL/6 mice were divided into (1) control; (2) DMM2; (3) DMM8; (4) rapamycin 2 weeks; and (5) rapamycin 8 weeks groups. Saffranin O-Fast green staining, histomorphometry and immunohistochemical methods were use...
Source: Autoimmunity - August 15, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Semaphorin-3A, semaphorin-7A gene single nucleotide polymorphisms, and systemic lupus erythematosus susceptibility.
Conclusions: Sema3A and Sema7A gene polymorphisms are not related to SLE genetic susceptibility, but may link to several clinical features of SLE. PMID: 31394943 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - August 11, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Distinct pattern of peripheral lymphocyte subsets in Graves' disease with persistency of anti-TSHR autoantibodies.
Conclusions: Using extensive phenotypic analysis of lymphocyte subpopulations, it is possible to detect changes that help to identify patients with persistent TSHR antibodies and may contribute to understand why the autoimmune response is maintained. PMID: 31366254 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - August 2, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Autoantibodies in Pandemrix ®-induced narcolepsy: Nine candidate autoantigens fail the conformational autoantibody test.
Conclusions: None of the nine labelled proteins proposed to be autoantigens were detected in the radiobinding assays for conformation-dependent autoantibodies. The results emphasise the need of further studies to identify autoantigen(s) and clarify the mechanisms in Pandemrix®-induced NT1. PMID: 31328572 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - July 23, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Blockade of the Notch1/Jagged1 pathway in Kupffer cells aggravates ischemia-reperfusion injury of orthotopic liver transplantation in mice.
Authors: Bai H, Wen J, Gong JP, Wu H, Yuan FC, Cao D, Wu YK, Lai X, Wang MH Abstract Liver ischemia-reperfusion injury (IRI) represents a risk factor for early graft dysfunction and an obstacle to expanding donor pool in orthotopic liver transplantation (OLT). Kupffer cells (KCs) are the largest antigen-presenting cell (APC) group and the primary modulators of inflammation in liver tissues. The vital role of Notch1/Jagged1 pathway in mouse OLT model has been reported, however, its potential therapeutic mechanism is unknown. Here, we made use of short hairpin RNA-Jagged1 and AAV-Jagged1 to explore the effects of Not...
Source: Autoimmunity - July 20, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Immune inhibitory proteins and their pathogenic and therapeutic implications in autoimmunity and autoimmune hepatitis.
In conclusion, deficiencies in key immune inhibitory proteins may promote the occurrence of autoimmune diseases, such as autoimmune hepatitis, and emerging interventions may overcome these deficiencies. Investigations should define the nature, impact and management of these inhibitory disturbances in autoimmune hepatitis. PMID: 31298041 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - July 13, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Neutrophil extracellular traps generation and degradation in patients with granulomatosis with polyangiitis and systemic lupus erythematosus.
Authors: Pruchniak MP, Ostafin M, Wachowska M, Jakubaszek M, Kwiatkowska B, Olesinska M, Zycinska K, Demkow U Abstract Neutrophils are one of the first cells to arrive at the site of infection, where they apply several strategies to kill pathogens: degranulation, respiratory burst, phagocytosis, and release of neutrophil extracellular traps (NETs). Recent discoveries try to connect NETs formation with autoimmune diseases, like systemic lupus erythematosus (SLE) or granulomatosis with polyangiitis (GPA) and place them among one of the factors responsible for disease pathogenesis. The aim of the study was to assess t...
Source: Autoimmunity - July 3, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Effects of intermittent T-cell cluster disaggregation on proliferative capacity and checkpoint marker expression.
Conclusions: These findings reveal new translation findings with respect to T-cell handling and production and suggest that T-cell disaggregation may be important to improved cell yields and phenotype. PMID: 31238751 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - June 28, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

MicroRNA-21 and microRNA-29a modulate the expression of collagen in dermal fibroblasts of patients with systemic sclerosis.
In this study, we investigated the expression pattern of 16 miRNAs, which have previously been confirmed or predicted to target genes involved in extracellular matrix (ECM) homeostasis. Primary culture of dermal fibroblasts was obtained from skin biopsies of diffused cutaneous SSc (dcSSc) patients and healthy controls. Expression of let-7a, miR-1, miR-15a, miR-17, miR-19a, miR-20a, miR-21, miR-27b, miR-26a, miR-29a, miR-29b, miR29c, miR-141, miR-125a-5p, miR-193a-3p, and miR-200a were quantified by Real-time PCR. Functional analysis of microRNAs was performed using synthetic oligonucleotides. To further confirm the pro- or...
Source: Autoimmunity - June 5, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Dihydrotestosterone regulates oxidative stress and immunosuppressive cytokines in a female BALB/c mouse model of Graves' disease.
Conclusions: The results demonstrated that DHT could reduce the severity of GD in female BALB/c mice by regulating oxidative stress. The upregulation of immunosuppressive cytokines might be another important protective mechanism. PMID: 31134819 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - May 30, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Crystalline silica activates the T-cell and the B-cell antigen receptor complexes and induces T-cell and B-cell proliferation.
Authors: Eleftheriadis T, Pissas G, Zarogiannis S, Liakopoulos V, Stefanidis I Abstract Silicosis is an occupational fibrotic lung disease, which is associated with an increased incidence of autoimmune diseases. The effect of crystalline silica on the immune system is thought to be mediated by the antigen presenting cells. However, the direct effect of silica on T-cells and B-cells has not been evaluated adequately. For this purpose, CD4(+)T-cells and B-cells from 10 healthy individuals were isolated and cultured with or without Min-U-Sil 5. Cell proliferation was assessed with BrdU assay. In cell proliferation exp...
Source: Autoimmunity - May 24, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Autoinflammatory disease with focus on NOD2-associated disease in the era of genomic medicine.
Authors: Yao Q, Li E, Shen B Abstract Systemic autoinflammatory diseases (SAIDs) represent a spectrum of genetically heterogeneous inflammatory disorders. Some SAID-associated genes are located in chromosome 16, including familial Mediterranean fever gene (MEFV) and nucleotide-binding oligomerization domain 2 [NOD2] gene that are linked to Crohn's disease, Blau syndrome, and Yao syndrome. These disorders share overlapping clinical phenotypes, and genotyping is diagnostically helpful and distinctive. Using next generation sequencing in SAIDs, digenic variants or combinations of more genetic variants in different gen...
Source: Autoimmunity - May 17, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Vitamin D supplementation ameliorates arthritis but does not alleviates renal injury in pristane-induced lupus model.
The objective of the present study was to evaluate the development and evolution of SLE after vitamin D supplementation in PIL model. Here, we evaluated the effects of vitamin D supplementation in model of pristane-induced SLE in female BALB/c mice. The animals were randomly divided into three groups: control group (CO), pristane-induced lupus group (PIL) and pristane-induced lupus group plus vitamin D (VD). Lupus was induced in PIL and VD groups using pristane. PIL group showed arthritis and kidney injury, characterized by increased proteinuria, glomerular mesangial expansion and inflammation. Moreover, PIL model showed i...
Source: Autoimmunity - May 17, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Group A streptococcal M-protein specific antibodies and T-cells drive the pathology observed in the rat autoimmune valvulitis model.
Authors: Sikder S, Price G, Alim MA, Gautam A, Scott Simpson R, Margaret Rush C, Lee Govan B, Ketheesan N Abstract Acute rheumatic fever (ARF) and rheumatic heart disease (RHD) are autoimmune mediated diseases triggered by group A streptococcal (GAS) infections. Molecular mimicry between GAS M-proteins and host tissue proteins has been proposed as the mechanism that initiates autoreactive immune responses in ARF/RHD. However, the individual role of antibodies and T-cells specific for GAS M-proteins in the pathogenesis of autoimmune carditis remains under-explored. The current study investigated the role of antibodi...
Source: Autoimmunity - May 9, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Immunoregulatory properties of cell-free DNA in plasma of celiac disease patients - A pilot study.
Authors: Brynychova I, Zinkova A, Hoffmanova I, Korabecna M, Dankova P Abstract The elevated plasma cell-free DNA (cfDNA) concentrations were repeatedly reported in association with the process of inflammation. The qualitative and quantitative characteristics of plasma cfDNA in active (newly diagnosed) celiac disease patients (CD) have not yet been studied despite the fact that cfDNA of healthy individuals is able to regulate immune response. We determined the total cfDNA concentration and relative content of telomeric sequences in plasma cfDNA in CD (n = 10) and healthy age- and sex-matched controls ...
Source: Autoimmunity - May 7, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

The association between rs1893217, rs478582 in PTPN2 and T1D risk with different diagnosed age, and related clinical characteristics in Chinese Han population.
CONCLUSIONS: Our results suggest that rs1893217 may increase the risk of early-onset T1D and affect humoral immunity, while rs478582 may affect Treg subsets. PMID: 31030572 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - April 30, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Selenium supplementation suppresses immunological and serological features of lupus in B6.Sle1b mice.
Authors: Soni C, Sinha I, Fasnacht MJ, Olsen NJ, Rahman ZSM, Sinha R Abstract Systemic lupus erythematosus (SLE) is a debilitating multi-factorial immunological disorder characterized by increased inflammation and development of anti-nuclear autoantibodies. Selenium (Se) is an essential trace element with beneficial anti-cancer and anti-inflammatory immunological functions. In our previous proteomics study, analysis of Se-responsive markers in the circulation of Se-supplemented healthy men showed a significant increase in complement proteins. Additionally, Se supplementation prolonged the life span of lupus prone N...
Source: Autoimmunity - April 24, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Editorial - NETs in autoimmune diseases.
Authors: Boeltz S, Herrmann M PMID: 30994384 [PubMed - in process] (Source: Autoimmunity)
Source: Autoimmunity - April 19, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Detection by flow cytometry of anti-neutrophil cytoplasmic antibodies in a novel approach based on neutrophil extracellular traps.
CONCLUSION: NET-coated microbeads may be commercially developed as a novel tool for automated ANCA screening assays using flow cytometry. PMID: 30994385 [PubMed - in process] (Source: Autoimmunity)
Source: Autoimmunity - April 19, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Reappraisal of antibodies against Saccharomyces cerevisiae (ASCA) as persistent biomarkers in quiescent Crohn's disease.
Authors: Duarte-Silva M, Afonso PC, de Souza PR, Peghini BC, Rodrigues-Júnior V, de Barros Cardoso CR Abstract A clear correlation exists between microbiota and the dysregulation of the immune response in Inflammatory Bowel Diseases (IBD), which comprise Crohn's disease (CD) and ulcerative colitis (UC). These unbalanced reactions also involve humoral responses, with antibodies against Saccharomyces cerevisiae. Thus, here we aimed to quantify IgA and IgG specific to S. cerevisiae (ASCA) in quiescent CD and UC, to correlate the production of these antibodies with patient's inflammatory response and disease cli...
Source: Autoimmunity - March 21, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Individual and stable autoantibody repertoires in healthy individuals.
Authors: Neiman M, Hellström C, Just D, Mattsson C, Fagerberg L, Schuppe-Koistinen I, Gummesson A, Bergström G, Kallioniemi O, Achour A, Sallinen R, Uhlén M, Nilsson P Abstract In the era towards precision medicine, we here present the individual specific autoantibody signatures of 193 healthy individuals. The self-reactive IgG signatures are stable over time in a way that each individual profile is recognized in longitudinal sampling. The IgG autoantibody reactivity towards an antigen array comprising 335 protein fragments, representing 204 human proteins with potential relevance to autoimmune dis...
Source: Autoimmunity - March 7, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

UBASH3A gene polymorphisms and expression profile in rheumatoid arthritis.
In this study, we explored the association between UBASH3A gene single-nucleotide polymorphisms (SNPs) and rheumatoid arthritis (RA) in a Chinese Han population. We also comparatively evaluated the UBASH3A expression profile in peripheral blood mononuclear cells (PBMCs) from patients with RA and healthy controls. METHODS: Four UBASH3A polymorphisms (rs1893592, rs11203203, rs2277798, and rs3788013) were studied in 553 patients with RA and 587 controls in a Chinese population. Genotyping was performed using the Fluidigm 192.24 Dynamic Array Integrated Fluidic Circuit (IFC). For gene expression study, UBASH3A mRNA levels ...
Source: Autoimmunity - March 4, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

CD5+ B lymphocytes secrete IL-10 rather than TGF- β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome.
CD5+ B lymphocytes secrete IL-10 rather than TGF-β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome. Autoimmunity. 2019 Feb 20;:1-9 Authors: Zhao M, Duan N, Wang Y, Zhu H, Liu H, Wang H, Xing L, Shao Z Abstract OBJECTIVE: To investigate the quantity and secretion function of cytokines-secreted CD5+ B lymphocytes in Autoimmune Haemolytic Anaemia (AIHA)/Evans syndrome (ES) patients. METHODS: Twenty-five untreated AIHA/ES patients, 28 remission AIHA/ES patients and 25 healthy controls (HCs) were enrolled in this study. The quantity of CD5+B lymphocytes which prod...
Source: Autoimmunity - February 22, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

The changes in immune cell concentration during the progression of pre-diabetes to type 2 diabetes in a high-fat high-carbohydrate diet-induced pre-diabetic rat model.
This study sought to characterize the changes in general immunity occurring during the progression from pre-diabetes to T2D. Male rats were fed a high-fat high-carbohydrate diet for 20 weeks (pre-diabetes induction period) and kept on the same diet being monitored for a further 12 weeks (experimental period). Blood was collected for haemocytometer analysis on week 0, 4, 8, and 12 of the experimental period after which the animals were sacrificed. Plasma was collected from centrifuged blood for ELISA (TNF-α, CRP, P-selectin, CD40 L, fibrinogen, and IL-6). Blood neutrophils percentage significantly...
Source: Autoimmunity - February 21, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Islet autoantibody positivity in overweight and obese adults with type 2 diabetes.
In conclusion, islet autoantibodies were present in 6.4% of overweight/obese adults with type 2 diabetes including those with severe obesity, and were associated with distinct clinical features. The effect of autoantibody positivity on weight loss interventions requires further study. PMID: 30661481 [PubMed - in process] (Source: Autoimmunity)
Source: Autoimmunity - January 23, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Immunotherapeutic effects of Mycobacterium tuberculosis rv3407 DNA vaccine in mice.
In this study, the gene encoding latency-associated antigen Rv3407 of Mycobacterium tuberculosis (MTB) rv3407 DNA vaccine was used to prepare and the immunogenicity and therapeutic effects were evaluated. Normal mice were immunized intramuscularly three times at two-week intervals with sterile water for injection, plasmid vector pVAX1, M. vaccae vaccine, ag85a DNA or rv3407 DNA. TB-infected mice were immunized intramuscularly three times at two-week intervals with phosphate-buffered saline (PBS) and rv3407 DNA. The normal mice immunized with rv3407 DNA or ag85a DNA showed higher levels of interferon-gamma (IFN-γ) in ...
Source: Autoimmunity - January 12, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Interleukin-6 and insulin-like growth factor-1 synergistically promote the progression of NSCLC.
Authors: Tang H, Bai Y, Pan G, Wang X, Wei Y, Yang Z, Zhao J Abstract The signaling pathways of interleukin-6 (IL-6) and insulin-like growth factor 1 (IGF-1) play an important role in the progression of lung cancer, and this study aimed to explore whether they can synergistically promote the progression of non-small cell lung cancer (NSCLC). We found that IL-6, glycoprotein 130 (GP130), IGF-1 and IGF-1R were highly expressed in NSCLC (p = .000), and there was the correlation between GP130, IGF-1, and IGF-1R (p 
Source: Autoimmunity - January 5, 2019 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

IL-37 gene variant (rs3811047): A marker of disease activity in rheumatoid arthritis: A pilot study.
CONCLUSION: IL-37 gene rs3811047 SNP was associated with more severe RA disease activity in the current population. Larger epidemiological study is warranted to validate our results. PMID: 30590949 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - December 30, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Bisphenol A: A notorious player in the mosaic of autoimmunity.
Authors: Aljadeff G, Longhi E, Shoenfeld Y Abstract The multifactorial etiology of autoimmune diseases has been studied at large. Genetic risk factors and environmental agents play an integral role in the pathogenesis of autoimmune processes. In recent decades, Bisphenol A (BPA), an exogenous compound found in polycarbonate plastic, has gained attention for its harmful multifocal effects on a diverse subset of systemic pathways, potentially contributing to disease onset and exacerbation. BPA is a xenoestrogen used globally in the manufacture of daily use products including plastic storage containers, water and infa...
Source: Autoimmunity - December 30, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Overexpression of toll-like receptor 9 correlates with podocyte injury in a murine model of autoimmune membranoproliferative glomerulonephritis.
Authors: Masum MA, Ichii O, Hosny Ali Elewa Y, Nakamura T, Otani Y, Hosotani M, Kon Y Abstract Toll-like receptors (Tlrs) are sensors of danger signals which promote the activation of immune cells and intrinsic renal cells. Podocytes, the intrinsic cells of glomerulus, are continuously exposed to various plasma solutes and danger signals due to their unique location in the glomerulus. Herein, we show that Tlr9 is overexpressed in podocytes and the mechanisms which cause its injury and development of membranoproliferative glomerulonephritis (MPGN) in model BXSB/MpJ-Yaa (Yaa) mice. Yaa mice developed typical lesions ...
Source: Autoimmunity - December 30, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Childhood thyroid autoimmunity and relation to islet autoantibodies in children at risk for type 1 diabetes in the diabetes prediction in sk åne (DiPiS) study.
Childhood thyroid autoimmunity and relation to islet autoantibodies in children at risk for type 1 diabetes in the diabetes prediction in skåne (DiPiS) study. Autoimmunity. 2018 Aug;51(5):228-237 Authors: Jonsdottir B, Larsson C, Lundgren M, Ramelius A, Jönsson I, Larsson HE, DiPiS study Group Abstract BACKGROUND: The aim was to determine prevalence and age at seroconversion of thyroid autoimmunity in relation to islet autoantibodies, gender and HLA-DQ genotypes in children with increased risk for type 1 diabetes followed from birth. METHODS: In 10-year-old children (n = 18...
Source: Autoimmunity - November 30, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Polymorphisms in Th17-related genes and the pathogenesis of autoimmune thyroid disease.
In conclusion, IL17R rs9607715 and IL17F rs763780 polymorphisms are associated with the susceptibility and severity of HD, respectively. IL21 rs907715, SOCS3 rs4969170 and CCR6 rs3093024 polymorphisms are associated with the intractability of GD. PMID: 30474404 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - November 27, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Tissue transglutaminase autoantibodies in children with newly diagnosed type 1 diabetes are related to human leukocyte antigen but not to islet autoantibodies: A Swedish nationwide prospective population-based cohort study.
This study explored the association between tissue transglutaminase autoantibody (tTGA), high-risk human leucocyte antigen (HLA) genotypes and islet autoantibodies in children with newly diagnosed type 1 diabetes (T1D). PATIENTS AND METHODS: Dried blood spots and serum samples were taken at diagnosis from children
Source: Autoimmunity - November 19, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Immunization with plasmids encoding M2 acetylcholine muscarinic receptor epitopes impairs cardiac function in mice and induces autophagy in the myocardium.
Authors: Ribeiro KC, Campelo RP, Rodrigues DDRF, Mattos EC, Brandão IT, da Silva CL, Bouskela E, Martinez CG, Kurtenbach E Abstract Autoantibodies against the M2 subtype of muscarinic acetylcholine receptors with functional activities have been found in the sera of patients with dilated cardiomyopathy (DCM), and the second extracellular loop has been established as the predominant epitope. However, it has been shown that the third intracellular loop is recognized by Chagas disease patients with severe cardiac dysfunction. In this work, BALB/c mice were immunized with plasmids encoding these two epitopes, and...
Source: Autoimmunity - November 16, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

TREX1 D18N mice fail to process erythroblast DNA resulting in inflammation and dysfunctional erythropoiesis.
Authors: Rego SL, Harvey S, Simpson SR, Hemphill WO, McIver ZA, Grayson JM, Perrino FW Abstract Anaemia is commonly observed in chronic inflammatory conditions, including systemic lupus erythematosus (SLE), where ∼50% of patients display clinical signs of anaemia. Mutation at the aspartate residue 18 of the three prime repair exonuclease 1 (TREX1) gene causes a monogenic form of cutaneous lupus in humans and the genetically precise TREX1 D18N mice recapitulate a lupus-like disease. TREX1 degrades single- and double-stranded DNA (dsDNA), and the link between failed DNA degradation by nucleases, including nucleos...
Source: Autoimmunity - November 15, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Periodontal sources of citrullinated antigens and TLR agonists related to RA.
Authors: Vitkov L, Hannig M, Minnich B, Herrmann M Abstract Anti-citrullinated protein autoantibodies (ACPA) precede the onset of clinical and subclinical rheumatoid arthritis (RA). ACPA are frequently generated in further chronic inflammatory diseases, e.g. chronic obstructive pulmonary disease, lupus, periodontitis (PD), characterized by citrullination and mucosal as well as systemic autoimmunity against citrullinated proteins. PD is of particular interest, as it exhibits two sources of citrullination, namely peptidylarginine deiminase 4 (PAD4) of periodontal neutrophils and neutrophil extracellular traps (NETs) ...
Source: Autoimmunity - November 13, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

Burning controversies in NETs and autoimmunity: The mysteries of cell death and autoimmune disease.
Authors: Dwivedi N, Radic M Abstract The causes and mechanisms of autoimmune disease pose continuing challenges to the scientific community. Recent clues implicate a peculiar feature of neutrophils, their ability to release nuclear chromatin in the form of neutrophil extracellular traps (NETs), in the induction or progression of autoimmune disease. Efforts to define the beneficial versus detrimental effects of NET release have, as yet, only partially revealed mechanisms that guide this process. Evidence suggests that the process of NET release is highly regulated, but the details of regulation remain controversial ...
Source: Autoimmunity - November 13, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research

New DiaP277 analogue shifts DCs to tolerogenic, and modulates NF-K β1 to suppress autoreactive T lymphocytes in the type 1 diabetic mice.
In conclusion, VP showed promising immune potential to modulate immune regulatory responses and shifts DCs to suppress autoreactive Th1 cells which ameliorated immunosuppressive potency in the type1 diabetic mice. PMID: 30382756 [PubMed - as supplied by publisher] (Source: Autoimmunity)
Source: Autoimmunity - November 3, 2018 Category: Allergy & Immunology Tags: Autoimmunity Source Type: research