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Infectious Disease: Parasitic Diseases
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Total 81 results found since Jan 2013.
Knocking down the expression of the molecular motors, myosin A, C and F genes in Toxoplasma gondii to decrease the parasite virulence
In conclusion, myosin proteins knock down proposes a promising therapeutic strategy to combat toxoplasmosis.PMID:37331576 | DOI:10.1016/j.exppara.2023.108565
Source: Experimental Parasitology - June 18, 2023 Category: Parasitology Authors: Tayebeh Zohrabi Mojtaba Azimi-Resketi Fereshteh Talaei Maryam Yaghoubi Mohamad Reza Ganjalikhany Farzaneh Mohamadi Farsani Abbasali Eskandarian Source Type: research
Effect of RNA interference with glutamate decarboxylase on acid resistance of Trichinella spiralis
Acta Trop. 2023 Feb 25:106869. doi: 10.1016/j.actatropica.2023.106869. Online ahead of print.ABSTRACTTrichinella spiralis is a zoonotic parasite that infects most mammals, even humans. Glutamate decarboxylase (GAD) is an important enzyme in glutamate-dependent acid resistance system 2 (AR2), but the GAD of T. spiralis in AR2 is unclear. We aimed to investigate the role of T. spiralis glutamate decarboxylase (TsGAD) in AR2. We silenced the TsGAD gene to evaluate the AR of T. spiralis muscle larvae (ML) in vivo and in vitro via siRNA. The results showed that recombinant TsGAD was recognized by anti-rTsGAD polyclonal antibody...
Source: Acta Tropica - February 27, 2023 Category: Infectious Diseases Authors: Jiaming Hou Shi Meng Bohan Zhang Rulin Ruan Yan Zhang Ze Wang Mingxin Song Zhikun Bai Source Type: research
Comparative proteomic profiles of Schistosoma japonicum male worms derived from single-sex and bisexual infections
In this study, the proteomic profiles of single-sex infected male (SM) worms and bisexual infected mated male (MM) worms of Schistosoma japonicum at 18, 21, 23, and 25 days p.i. were identified through data-independent acquisition. In total, 674 differentially expressed proteins (DEPs) were identified for the SM and MM worms at all four timepoints. Bioinformatic analysis demonstrated that most of the DEPs were involved in biosynthetic processes including locomotion, cell growth and death, cell motility, and metabolic processes such as protein metabolism and glucose metabolism. Schistosoma japonicum glycosyltransferase (SjG...
Source: International Journal for Parasitology - October 20, 2022 Category: Parasitology Authors: Haoran Zhong Luobin Wu Yuqi Ren Fanglin Qin Yamei Jin Source Type: research
Molecular characterization and functional analysis of Schistosoma mekongi neuroglobin homolog
In this study, we aimed to characterize the molecular properties and functions of Schistosoma mekongi Ngb (SmeNgb) using bioinformatic, biochemical, and molecular biology approaches. The amino acid sequence of Ngb was highly conserved among schistosomes as well as closely related trematodes. SmeNgb was abundantly localized in the gastrodermis, vitelline, and ovary of adult female S. mekongi worms as well as in the tegument of adult male worms. Assessment of antioxidant activity demonstrated that recombinant SmeNgb had Fe2+ chelating and hydrogen peroxide scavenging activities. Intriguingly, siRNA silencing of SmeNgb gene e...
Source: Acta Tropica - April 1, 2022 Category: Infectious Diseases Authors: Orawan Phuphisut Porntida Kobpornchai Phiraphol Chusongsang Yanin Limpanont Tapanee Kanjanapruthipong Sumate Ampawong Onrapak Reamtong Poom Adisakwattana Source Type: research
Schistosoma japonicum translationally controlled tumor protein, which is associated with the development of female worms, as a target for control of schistosomiasis
In this study, the proteomic profiles of single-sex infected female (SF) worms and bisexual infected mature female (MF) worms of Schistosoma japonicum at 18, 21, 23 and 25 days p.i. were identified with isobaric tags for relative quantitation-coupled liquid chromatography-tandem mass spectrometry. Differentially expressed proteins (DEPs) were subsequently used for bioinformatic analysis. Six highly expressed DEPs in MF worms were selected and long-term interference with small interfering RNA (siRNA) was conducted to determine biological functions. SiRNA against S. japonicum translationally controlled tumor protein (SjTCTP)...
Source: International Journal for Parasitology - March 23, 2022 Category: Parasitology Authors: Haoran Zhong Yuqi Ren Fanglin Qin Xiaochun Li Ling Hou Shaopeng Gu Yamei Jin Source Type: research
CFIm-mediated alternative polyadenylation remodels cellular signaling and miRNA biogenesis
We describe the elicited signaling pathways downstream of CFIm, which improve our understanding of the key role of CFIm in integrating RNA processing with other cellular activities.PMID:35234914 | DOI:10.1093/nar/gkac114
Source: Cell Research - March 2, 2022 Category: Cytology Authors: Souvik Ghosh Meric Ataman Maciej Bak Anastasiya B örsch Alexander Schmidt Katarzyna Buczak Georges Martin Beatrice Dimitriades Christina J Herrmann Alexander Kanitz Mihaela Zavolan Source Type: research
Complex Small RNA-mediated Regulatory Networks between Viruses/Viroids/Satellites and Host Plants
Virus Res. 2022 Feb 6:198704. doi: 10.1016/j.virusres.2022.198704. Online ahead of print.ABSTRACTHost plants deploy the small RNA (sRNA)-directed RNA silencing pathway to resist invasion by acellular microorganisms (viruses/viroids/satellites), and, in turn, this pathway is exploited by pathogenic agents to create an environment conducive to infection. Previous known sRNA-RNA systems consist of host endogenous microRNAs (miRNAs) mediating the regulation of host mRNAs and virus/viroid/satellite-derived small interfering RNAs (vsiRNAs) targeting their genomic RNAs. However, more in-depth explorations have substantially expan...
Source: Virus Research - February 9, 2022 Category: Virology Authors: Chaonan Wang Fan Jiang Shuifang Zhu Source Type: research
< i > Trichinella spiralis < /i > : Knockdown of gamma interferon inducible lysosomal thiol reductase (GILT) results in the reduction of worm burden
by Hong Fei, Muhammad Ali-ul-Husnain Naqvi, Sana Zahra Naqvi, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng YanTrichinella spiralis is mammalian skeletal muscles parasite which may cause trichinellosis in animals and humans. Gamma interferon inducible lysosomal thiol reductase (GILT) is a widespread superfamily which plays key role in processing and presentation of MHC class II restricted antigen by catalyzing disulfide bond reduction. There are no reports about GILT inT.spiralis. In present study, GILT fromT.spiralis (Tsp-GILT) was cloned, analyzed by multiple-sequence alignment, and predicted by 3D structure model. Recomb...
Source: PLoS Neglected Tropical Diseases - November 30, 2021 Category: Tropical Medicine Authors: Hong Fei Source Type: research
