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Condition: Glaucoma

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Total 87 results found since Jan 2013.

Receptor potential channels TRPC1/TRPC6 regulate lamina cribrosa cell extracellular matrix gene transcription and proliferation.
In conclusion, TRPC1/C6 expression is enhanced in glaucoma LC cells. These channels may contribute to oxidative stress-induced ECM gene transcription and cell proliferation in normal and glaucoma LC cells through Ca2+-NFATc3 signaling pathway mechanism. TRPC1 and TRPC6 channels could be important therapeutic targets to prevent ECM remodeling and fibrosis development in glaucoma optic neuropathy. PMID: 32088241 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - February 19, 2020 Category: Opthalmology Authors: Irnaten M, O'Malley G, Clark AF, O'Brien CJ Tags: Exp Eye Res Source Type: research

RNA therapeutics in ophthalmology - translation to clinical trials.
Abstract The use of RNA interference technology has proven to inhibit the expression of many target genes involved in the underlying pathogenesis of several diseases affecting various systems. First established in in vitro and later in animal studies, small interfering RNA (siRNA) and antisense oligonucleotide (ASO) therapeutics are now entering clinical trials with the potential of clinical translation to patients. Gene-silencing therapies have demonstrated promising responses in ocular disorders, predominantly due to the structure of the eye being a closed and compartmentalised organ. However, although the effic...
Source: Experimental Eye Research - February 3, 2021 Category: Opthalmology Authors: Gupta A, Kafetzis KN, Tagalakis AD, Yu-Wai-Man C Tags: Exp Eye Res Source Type: research

Activated {alpha}v{beta}3 Integrin Regulates {alpha}v{beta}5 Integrin-Mediated Phagocytosis in Trabecular Meshwork Cells Glaucoma
Conclusions. The αvβ5 integrin-FAK–mediated pathway regulates phagocytosis in TM cells and this pathway is inhibited by activation of αvβ3 integrins. This suggests that changes in integrin expression and activity may be responsible for alterations in phagocytosis observed in steroid induced glaucoma.
Source: Investigative Ophthalmology - July 24, 2013 Category: Opthalmology Authors: Gagen, D., Filla, M. S., Clark, R., Liton, P., Peters, D. M. Tags: Glaucoma Source Type: research

Lithium chloride inhibits TGF-β1-induced myofibroblast transdifferentiation via PI3K/Akt pathway in cultured fibroblasts from Tenon's capsule of the human eye.
Abstract Excess scarring of the conjunctiva after glaucoma filtration surgery is a major cause of failure. Transforming growth factor (TGF)-β is critically involved in post-operative scarring. Lithium inhibits TGF-β-induced gene protein expression in corneal fibroblasts and inhibits TGF-β-induced epithelial mesenchymal transition. Here, we investigated the effects of LiCl on TGF-β1-mediated signaling pathways and on myofibroblast transdifferentiation of human Tenon's capsule fibroblasts (HTFs). LiCl treatment reduced expression of TGF-β1-induced α-SMA expression in HTFs. LiCl also decreased Akt phosphorylati...
Source: Biotechnology Letters - February 22, 2014 Category: Biotechnology Authors: Chung EJ, Sohn YH, Kwon SH, Jung SA, Lee JH Tags: Biotechnol Lett Source Type: research

Latent TGF-{beta} binding protein-2 is essential for the development of ciliary zonule microfibrils
In this study, we explore the in vivo function of LTBP-2 by generating Ltbp2–/– mice. Ltbp2–/– mice survived to adulthood but developed lens luxation caused by compromised ciliary zonule formation without a typical phenotype related to glaucoma, suggesting that LTBP-2 deficiency primarily causes lens dislocation but not glaucoma. The suppression of LTBP2 expression in cultured human ciliary epithelial cells by siRNA disrupted the formation of the microfibril meshwork by the cells. Supplementation of recombinant LTBP-2 in culture medium not only rescued the microfibril meshwork formation in LTBP2-sup...
Source: Human Molecular Genetics - October 8, 2014 Category: Genetics & Stem Cells Authors: Inoue, T., Ohbayashi, T., Fujikawa, Y., Yoshida, H., Akama, T. O., Noda, K., Horiguchi, M., Kameyama, K., Hata, Y., Takahashi, K., Kusumoto, K., Nakamura, T. Tags: ARTICLES Source Type: research

Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells
Conclusion: Down-regulating miR-100 is an effective method to protect H2O2 induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation.
Source: BMC Molecular Biology - November 18, 2014 Category: Molecular Biology Authors: Ning KongXiaohe LuBin Li Source Type: research

Crosstalk between TGFβ and Wnt signaling pathways in the human trabecular meshwork.
In this study, we determined whether there is a crosstalk between the TGFβ/Smad pathway and the canonical Wnt pathway using luciferase reporter assays. Lentiviral luciferase reporter vectors for studying the TGFβ/Smad pathway or the canonical Wnt pathway were transduced into primary human non-glaucomatous TM (NTM) cells. Cells were treated with or without a combination of 5 μg/ml TGFβ2 and/or 100 ng/ml Wnt3a recombinant proteins, and luciferase levels were measured using a plate reader. We found that TGFβ2 inhibited Wnt3a-induced canonical Wnt pathway activation, while Wnt3a inhibited TGFβ2-induced TGFβ/Smad pathw...
Source: Experimental Eye Research - April 13, 2016 Category: Opthalmology Authors: Webber HC, Bermudez JY, Sethi A, Clark AF, Mao W Tags: Exp Eye Res Source Type: research

Vertebrate Lonesome Kinase Regulated Extracellular Matrix Protein Phosphorylation, Cell Shape and Adhesion in Trabecular Meshwork Cells
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Physiology - September 4, 2016 Category: Cytology Authors: Rupalatha Maddala, Nikolai P. Skiba, Ponugoti Vasantha Rao Tags: Original Research Article Source Type: research

Vertebrate Lonesome Kinase Regulated Extracellular Matrix Protein Phosphorylation, Cell Shape, and Adhesion in Trabecular Meshwork Cells
Glaucoma, a leading cause of irreversible blindness, is commonly associated with elevated intraocular pressure (IOP) due to impaired aqueous humor (AH) drainage through the trabecular meshwork (TM). Although dysregulated production and organization of extracellular matrix (ECM) is presumed to increase resistance to AH outflow and elevate IOP by altering TM cell contractile and adhesive properties, it is not known whether regulation of ECM protein phosphorylation via the secretory vertebrate lonesome kinase (VLK) influences TM cellular characteristics. Here, we tested this possibility. Experiments carried out in this study ...
Source: Journal of Cellular Physiology - March 27, 2017 Category: Cytology Authors: Rupalatha Maddala, Nikolai P. Skiba, Ponugoti Vasantha Rao Tags: Original Research Article Source Type: research

Long non-coding RNA GAS5 contributed to the development of glaucoma via regulating the TGF- β signaling pathway.
CONCLUSIONS: In our study, we found that down-regulation of lncRNA GAS5 may maintain retinal ganglion cell survival in glaucoma through the activation of TGF-β pathway to promote cell proliferation and differentiation. PMID: 29509235 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

LTBP2 knockdown and oxidative stress affect glaucoma features including TGF β pathways, ECM genes expression and apoptosis in trabecular meshwork cells.
LTBP2 knockdown and oxidative stress affect glaucoma features including TGFβ pathways, ECM genes expression and apoptosis in trabecular meshwork cells. Gene. 2018 Jun 13;: Authors: Suri F, Yazdani S, Elahi E Abstract Glaucoma is the leading cause of irreversible blindness worldwide. Although the etiology of glaucoma is incompletely understood, it is known that the extracellular matrix (ECM) of the trabecular meshwork, oxidative stress, TGFβ signaling pathways, and apoptosis are important components of glaucoma pathogenesis. These components appear to be interrelated, but knowledge on their interacti...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Suri F, Yazdani S, Elahi E Tags: Gene Source Type: research

Interleukin-6-mediated trans-signaling inhibits transforming growth factor-{beta} signaling in trabecular meshwork cells Molecular Bases of Disease
Glaucoma is one of the major causes of blindness, and transforming growth factor-β2 (TGF-β2) has been found to be elevated in the aqueous humor of eyes with primary open-angle glaucoma (POAG). TGF-β2 in aqueous humor causes the glaucoma-related fibrosis of human trabecular meshwork (HTM), suggesting an important role of TGF-β in POAG pathogenesis. Here, we sought to elucidate the effects of IL-6 trans-signaling on TGF-β signaling in HTM cells. Using a multiplex immunoassay, POAG patients decreased IL-6 levels and increased soluble IL-6 receptor (sIL-6R) levels compared with the controls. In in vitro experiments, we ob...
Source: Journal of Biological Chemistry - July 13, 2018 Category: Chemistry Authors: Miyuki Inoue-Mochita, Toshihiro Inoue, Sachi Kojima, Akiko Futakuchi, Tomokazu Fujimoto, Saori Sato-Ohira, Utako Tsutsumi, Hidenobu Tanihara Tags: Cell Biology Source Type: research

Targeted therapy for the post-operative conjunctiva: SPARC silencing reduces collagen deposition.
CONCLUSIONS: SPARC targeted therapy effectively reduced both SPARC and collagen production in the operated mouse conjunctiva. This proof-of-concept study suggests that targeted treatment of fibrosis in glaucoma surgery is safe and feasible, with the potential to extend to a range of potential genes associated with fibrosis. PMID: 30021812 [PubMed - as supplied by publisher]
Source: The British Journal of Ophthalmology - July 18, 2018 Category: Opthalmology Authors: Seet LF, Tan YF, Toh LZ, Chu SW, Lee YS, Venkatraman SS, Wong TT Tags: Br J Ophthalmol Source Type: research

Contribution of the clock gene DEC2 to VEGF mRNA upregulation by modulation of HIF1 α protein levels in hypoxic MIO-M1 cells, a human cell line of retinal glial (Müller) cells
ConclusionDEC2 could upregulate retinalVEGF gene expression through modulation of HIF1 α levels under hypoxic conditions.
Source: Japanese Journal of Ophthalmology - September 24, 2018 Category: Opthalmology Source Type: research