Filtered By:
Cancer: Neuroblastoma

This page shows you your search results in order of date. This is page number 13.

Order by Relevance | Date

Total 288 results found since Jan 2013.

RGS2 modulates the activity and internalization of dopamine D2 receptors in neuroblastoma N2A cells.
Abstract Dysregulated expression and function of dopamine D2 receptors (D2Rs) are implicated in drug addiction, Parkinson's disease and schizophrenia. In the current study, we examined whether D2Rs are modulated by regulator of G protein signaling 2 (RGS2), a member of the RGS family that regulates G protein signaling via acceleration of GTPase activity. Using neuroblastoma 2a (N2A) cells, we found that RGS2 was immunoprecipitated by aluminum fluoride-activated Gαi2 proteins. RGS2 siRNA knockdown enhanced membrane [(35)S] GTPγS binding to activated Gαi/o proteins, augmented inhibition of cAMP accumulation and i...
Source: Neuropharmacology - August 11, 2016 Category: Drugs & Pharmacology Authors: Luessen DJ, Hinshaw TP, Sun H, Howlett AC, Marrs G, McCool BA, Chen R Tags: Neuropharmacology Source Type: research

Inhibition of sphingomyelin synthase 1 affects ceramide accumulation and hydrogen peroxide-induced apoptosis in Neuro-2a cells
Oxidative stress plays a key role in brain injury after cerebral ischemia–reperfusion, which contributes toward excessive apoptosis of nerve cells. Therefore, it would be beneficial to identify a therapy that could interfere with the progression of apoptosis and protect the brain from ischemia–reperfusion injury. As ceramide, a well-known second messenger of apoptosis, can be metabolized by sphingomyelin synthase 1 (SMS1), recent research has focused on the link between SMS1 and apoptosis in different cells. To investigate whether SMS1 is involved in the process of oxidative stress-induced apoptosis in neurons and to e...
Source: NeuroReport - August 4, 2016 Category: Neurology Tags: Molecular Biology Source Type: research

Melatonin antagonizes cadmium ‐induced neurotoxicity by activating the transcription factor EB‐dependent autophagy–lysosome machinery in mouse neuroblastoma cells
Abstract Cadmium (Cd), a highly ubiquitous heavy metal, induces neurotoxicity. Melatonin, a major secretory product of the pineal gland, protects against Cd‐induced neurotoxicity. However, the mechanism that accounts for this protection remains to be elucidated. Herein, we exposed mouse neuroblastoma cells (Neuro‐2a cells) to different concentrations of cadmium chloride (CdCl2) (12.5, 25, and 50 μ mol L−1) for 24 hours. We showed that Cd inhibits autophagosome–lysosome fusion and impairs lysosomal function, subsequently leading to nerve cell death. In addition, Cd decreases the level of transcription factor EB...
Source: Journal of Pineal Research - August 2, 2016 Category: Research Authors: Min Li, Huifeng Pi, Zhiqi Yang, Russel J. Reiter, Shangcheng Xu, Xiaowei Chen, Chunhai Chen, Lei Zhang, Min Yang, Yuming Li, Pan Guo, Gaoming Li, Manyu Tu, Li Tian, Jia Xie, Mindi He, Yonghui Lu, Min Zhong, Yanwen Zhang, Zhengping Yu, Zhou Zhou Tags: Original Article Source Type: research

Protection from interferon ‐β‐induced neuronal apoptosis through stimulation of muscarinic acetylcholine receptors coupled to ERK1/2 activation
Conclusions and implicationsStimulation of mAChRs counteracted IFN‐β‐induced neuronal apoptosis through the activation of ERK1/2 signalling. The data indicate that activation of ERK1/2‐coupled mAChRs may be an effective strategy for preventing IFNs neurotoxicity. This article is protected by copyright. All rights reserved.
Source: British Journal of Pharmacology - July 30, 2016 Category: Drugs & Pharmacology Authors: Maria C. Olianas, Simona Dedoni, Pierluigi Onali Tags: RESEARCH PAPER Source Type: research

Overexpression of MYCN promotes proliferation of non-small cell lung cancer
Abstract V-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (MYCN) is an oncogene that is known amplified and overexpressed in different human malignancies including small cell lung cancer. However, the role of MYCN in non-small cell lung cancer (NSCLC) development remains elusive. In the present study, Western blot and immunohistochemistry assays demonstrated that MYCN was overexpressed in NSCLC tumor tissues and cell lines. In addition, immunohistochemistry analysis revealed that upregulation of MYCN expression was positively correlated with a more invasive tumor phenotype and poor prognosis. In v...
Source: Tumor Biology - July 22, 2016 Category: Cancer & Oncology Source Type: research

Melatonin antagonizes cadmium‐induced neurotoxicity by activating the transcription factor EB ‐dependent autophagy‐lysosome machinery in mouse neuroblastoma cells
This article is protected by copyright. All rights reserved.
Source: Journal of Pineal Research - July 11, 2016 Category: Research Authors: Min Li, Huifeng Pi, Zhiqi Yang, Russel J. Reiter, Shangcheng Xu, Xiaowei Chen, Chunhai Chen, Lei Zhang, Yang Min, Yuming Li, Pan Guo, Gaoming Li, Manyu Tu, Li Tian, Jia Xie, Mindi He, Yonghui Lu, Min Zhong, Yanwen Zhang, Zhengping Yu, Zhou Zhou Tags: Original Article Source Type: research

Melatonin antagonizes cadmium ‐induced neurotoxicity by activating the transcription factor EB ‐dependent autophagy‐lysosome machinery in mouse neuroblastoma cells
This article is protected by copyright. All rights reserved.
Source: Journal of Pineal Research - July 9, 2016 Category: Research Authors: Min Li, Huifeng Pi, Zhiqi Yang, Russel J. Reiter, Shangcheng Xu, Xiaowei Chen, Chunhai Chen, Lei Zhang, Yang Min, Yuming Li, Pan Guo, Gaoming Li, Manyu Tu, Li Tian, Jia Xie, Mindi He, Yonghui Lu, Min Zhong, Yanwen Zhang, Zhengping Yu, Zhou Zhou Tags: Original Article Source Type: research

ERP44 inhibits human lung cancer cell migration mainly via IP3R2.
In this study, we used the human lung adenocarcinoma A549 cell line to examine the role of endoplasmic reticulum protein 44 (ERP44), which has been reported to regulate calcium release inside of the endoplasmic reticulum (ER), in cell migration. We found that the inositol 1,4,5-trisphosphate receptors (IP3Rs/ITPRs) inhibitor 2-APB significantly inhibited A549 cell migration by inhibiting cell polarization and pseudopodium protrusion, which suggests that Ca2+ is necessary for A549 cell migration. Similarly, the overexpression of ERP44 reduced intracellular Ca2+ release via IP3Rs, altered cell morphology and significantly in...
Source: Aging - June 22, 2016 Category: Biomedical Science Authors: Huang X, Jin M, Chen YX, Wang J, Zhai K, Chang Y, Yuan Q, Yao KT, Ji G Tags: Aging (Albany NY) Source Type: research

Botanical Polyphenols Mitigate Microglial Activation and Microglia-Induced Neurotoxicity: Role of Cytosolic Phospholipase A 2
In this study, we investigate the action of botanical compounds and assess whether suppression of cPLA2 in microglia is involved in the neurotoxic effects on neurons. Differentiated SH-SY5Y neuroblastoma cells were used to test the neurotoxicity of conditioned medium from stimulated microglial cells, and WST-1 assay was used to assess for the cell viability of SH-SY5Y cells. Botanicals such as quercetin and honokiol (but not cyanidin-3-O-glucoside, 3CG) were effective in inhibiting LPS-induced nitric oxide (NO) production and phosphorylation of cPLA2. Conditioned medium from BV-2 cells stimulated with LPS or IFNγ caused n...
Source: NeuroMolecular Medicine - June 22, 2016 Category: Neurology Source Type: research

Homocysteine induces PUMA-mediated mitochondrial apoptosis in SH-SY5Y cells.
In this study, we investigated the molecular pathway of homocysteine-induced apoptosis in caspase-4 deficient SH-SY5Y human neuroblastoma cells. Homocysteine significantly increased mRNA levels of CHOP and p53, resulting in the upregulation of their downstream target gene, p53 up-regulated modulator of apoptosis (PUMA). In cells treated with homocysteine, Bcl-2-associated X protein (BAX) protein levels, cytochrome c release from the mitochondria, and caspase-9 activation were significantly increased. Consistently, a caspase-9 inhibitor significantly alleviated homocysteine-induced cytotoxicity. Significantly lower BAX mRNA...
Source: Amino Acids - June 22, 2016 Category: Biochemistry Authors: Jang Y, Kim J, Ko JW, Kwon YH Tags: Amino Acids Source Type: research

Glycine N-acyltransferase-like 3 is responsible for long-chain N-acylglycine formation in N18TG2 cells Research Articles
Long-chain fatty acid amides are signaling lipids found in mammals and other organisms; however, details of the metabolic pathways for the N-acylglycines and primary fatty acid amides (PFAMs) have remained elusive. Heavy-labeled precursor and subtraction lipidomic experiments in mouse neuroblastoma N18TG2 cells, a model cell line for the study of fatty acid amide metabolism, establish the biosynthetic pathways for the N-acylglycines and the PFAMs. We provide evidence that the N-acylglycines are formed by a long-chain specific glycine-conjugating enzyme, glycine N-acyltransferase-like 3 (GLYATL3). siRNA knockdown of GLYATL3...
Source: The Journal of Lipid Research - April 30, 2016 Category: Lipidology Authors: Jeffries, K. A., Dempsey, D. R., Farrell, E. K., Anderson, R. L., Garbade, G. J., Gurina, T. S., Gruhonjic, I., Gunderson, C. A., Merkler, D. J. Tags: Research Articles Source Type: research

Abstract B31: Combined siRNA and small molecule screening identifies Aurora B kinase as an effective target in MYCN-driven neuroblastoma
Despite advances in multimodal treatment, neuroblastoma (NB) is often fatal for children with high-risk disease and many survivors need to cope with long-term side effects from high-dose chemotherapy and radiation. To identify new therapeutic targets, we performed a siRNA screen of the druggable genome combined with a small molecule screen of 465 compounds targeting 39 different mechanisms of actions in four NB cell lines. We identified 58 genes as targets, including AURKB, in at least one cell line. In the drug screen, aurora kinase inhibitors (nine molecules) and in particular the AURKB-selective compound, barasertib, we...
Source: Cancer Research - April 3, 2016 Category: Cancer & Oncology Authors: Bogen, D., Wei, J. S., Azorsa, D. O., Ormanoglu, P., Buehler, E., Guha, R., Keller, J. M., Griner, L. A. M., Ferrer, M., Song, Y. K., Liao, H., Mendoza, A., Gryder, B. E., Sindri, S., He, J., Wen, X., , Zhang, S., Shern, J. F., Yohe, M. E., Taschner-Mandl Tags: Targeted Therapeutics and Resistance Source Type: research

SARAF Regulates ARC Channels Cell Biology
The store-operated Ca2+ entry-associated regulatory factor (SARAF) has recently been identified as a STIM1 regulatory protein that facilitates slow Ca2+-dependent inactivation of store-operated Ca2+ entry (SOCE). Both the store-operated channels and the store-independent arachidonate-regulated Ca2+ (ARC) channels are regulated by STIM1. In the present study, we show that, in addition to its location in the endoplasmic reticulum, SARAF is constitutively expressed in the plasma membrane, where it can interact with plasma membrane (PM)-resident ARC forming subunits in the neuroblastoma cell line SH-SY5Y. Using siRNA-based and...
Source: Journal of Biological Chemistry - March 24, 2016 Category: Chemistry Authors: Albarran, L., Lopez, J. J., Woodard, G. E., Salido, G. M., Rosado, J. A. Tags: Signal Transduction Source Type: research

Long non-coding RNA HOTAIR promotes Parkinson's disease induced by MPTP through up-regulating the expression of LRRK2.
In conclusion, high expression of HOTAIR promoted the onset of PD induced by MPTP. Moreover, the finding that HOTAIR promoted PD induced by MPTP through regulating LRRK2 expression could add our understanding of the molecular mechanisms in PD. PMID: 26979073 [PubMed - as supplied by publisher]
Source: Current Neurovascular Research - March 16, 2016 Category: Neurology Authors: Liu S, Cui B, Dai ZX, Shi PK, Wang ZH, Guo YY Tags: Curr Neurovasc Res Source Type: research

MicroRNA-141 and its associated gene FUS modulate proliferation, migration and cisplatin chemosensitivity in neuroblastoma cell lines.
Authors: Wang Z, Lei H, Sun Q Abstract In the present study, a novel signaling pathway of microRNA-141 (miR-141)/fused in sarcoma (FUS) was investigated in neuroblastoma (NB). Gene expression of miR-141 was evaluated in 6 NB cell lines. IMR-32 and SH-SY5Y cells were transduced with the miR-141 mimic lentivirus. The effects of miR-141 upregulation on cell proliferation, cell division, migration, chemosensitivity and in vivo explants were evaluated by MTT, cell cycle, wound-healing, cisplatin sensitivity and in vivo tumor growth assays, respectively. The correlation between miR-141 and the FUS gene was evaluated b...
Source: Oncology Reports - March 6, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research