< em > Lskipk Lsatpase < /em > double mutants are necessary and sufficient for the compact plant architecture of butterhead lettuce
This study provides new gene resources and theory for the breeding of the crop ideotype.PMID:38371637 | PMC:PMC10873588 | DOI:10.1093/hr/uhad280 (Source: Cell Research)
Source: Cell Research - February 19, 2024 Category: Cytology Authors: Sai Xie Guangbao Luo Guanghui An Bincai Wang Hanhui Kuang Xin Wang Source Type: research

Evaluation of different QuEChERS-based methods for the extraction of 48 wastewater-derived organic contaminants from soil and lettuce root using high-resolution LC-QTOF with MRM < sup > HR < /sup > and SWATH acquisition modes
In this study, a modified QuEChERS-based approach was developed for rapid, simple, and simultaneous extraction of 48 organic wastewater-derived contaminants from soil and lettuce root. Twenty-two different (modification) scenarios of the known (or original) QuEChERS method have been tested, in order to obtain best and well-compromised recoveries for all target compounds for soil and roots. Finally, a common method was chosen for both matrices consisting of a single extraction step using EDTA-Mcllvaine buffer and the unbuffered Original QuEChERS salts. Method performance was accomplished by liquid chromatography coupled wit...
Source: Environmental Science and Pollution Research International - February 19, 2024 Category: Environmental Health Authors: Nicola Montemurro Rayana Manasfi Serge Chiron Sandra Perez Source Type: research

Antibiotic Resistance Profile of < em > E. coli < /em > Isolates from Lettuce, Poultry Manure, Irrigation Water, and Soil in Kumasi, Ghana
Int J Microbiol. 2024 Jan 27;2024:6681311. doi: 10.1155/2024/6681311. eCollection 2024.ABSTRACTInputs such as irrigation water and poultry manure used in lettuce cultivation have been found to be associated with antibiotic-resistant pathogens. The study assessed the antibiotic resistance profile of Escherichia coli isolated from lettuce, poultry manure, irrigation water, and soil in Kumasi. One hundred and fifty-six samples of lettuce, irrigation water, soil, and manure were collected from three farms over a seven-week cultivation period (seedlings to harvest stage). E. coli were enumerated using standard methods. 98% of t...
Source: International Journal of Microbiology - February 5, 2024 Category: Microbiology Authors: Abigail Abena Anokyewaa Appau Linda Aurelia Ofori Source Type: research

A hyperspectral deep learning attention model for predicting lettuce chlorophyll content
The phenotypic traits of leaves are the direct reflection of the agronomic traits in the growth process of leafy vegetables, which plays a vital role in the selection of high-quality leafy vegetable varieties.... (Source: Plant Methods)
Source: Plant Methods - February 3, 2024 Category: Biology Authors: Ziran Ye, Xiangfeng Tan, Mengdi Dai, Xuting Chen, Yuanxiang Zhong, Yi Zhang, Yunjie Ruan and Dedong Kong Tags: Research Source Type: research

A systematic review and meta-analysis indicates a high risk of human noroviruses contamination in vegetable worldwide, with GI being the predominant genogroup
This study aimed to assess the HuNoVs prevalence in vegetables. We searched the Web of Science, Excerpta Medica Database, PubMed, and Cochrane databases until June 1, 2023. A total of 27 studies were included for the meta-analysis. Statistical analysis was conducted using Stata 14.0 software. This analysis showed that the pooled HuNoVs prevalence in vegetables was 7 % (95 % confidence interval (CI): 3-13) worldwide. The continent with largest number of studies was Europe, and the highest number of samples was lettuce. As revealed by the results of the subgroup meta-analysis, the prevalence of GI genogroup was the highest (...
Source: International Journal of Food Microbiology - February 2, 2024 Category: Food Science Authors: Junshan Gao Liang Xue Yijing Li Jumei Zhang Jingsha Dai Qinghua Ye Shi Wu Qihui Gu Youxiong Zhang Xianhu Wei Qingping Wu Source Type: research

Microbiological evaluation and possible origins of the microbial contamination of vegetables in Ouagadougou (Burkina Faso)
This study aimed to contribute to the health safety of widely consumed vegetables in Ouagadougou by investigating the potential contamination of onion, tomato, cucumber and lettuce with pathogenic microorganisms. A survey was conducted, involving 102 producers in fields, 102 vendors in markets, and 205 consumers. The most commonly consumed vegetables were identified as onion, tomato, cucumbe... (Source: African Journal of Microbiology Research)
Source: African Journal of Microbiology Research - January 31, 2024 Category: Microbiology Source Type: research

A New Triterpenoid from Gymnema sylvestre and its Allelopathic Activity
A new triterpenoid (1) and four known compounds (2 –5) were isolated fromGymnema sylvestre. The structure of compound1 was elucidated as (3β,16β,22α)-22-benzoyloxy-16,23,28,29-tetrahydroxyolean-12-en-3-yl-O-β-D-glucopyranosiduronic acid by spectroscopic data (1H,13C NMR, HMBC, HSQC, COSY, and NOESY). Additionally, compound1 presented allelopathic activity on the growth of lettuce at 100 μM. (Source: Chemistry of Natural Compounds)
Source: Chemistry of Natural Compounds - January 30, 2024 Category: Chemistry Source Type: research

Hypervirulent clonal complex (CC) of Listeria monocytogenes in fresh produce from urban communities
This study aimed to determine the prevalence and virulome of Listeria in fresh produce distributed in urban communities.MethodsA total of 432 fresh produce samples were collected from farmer’s markets in Michigan and West Virginia, USA, resulting in 109 pooled samples. Listeria spp. were isolated and L. monocytogenes was subjected to genoserogrouping by PCR and genotyping by pulsed-field gel electrophoresis (PFGE). Multi-locus sequence typing (MLST) and core-genome multi-locus sequence typing (cgMLST) were conducted for clonal identification.ResultsForty-eight of 109 samples (44.0%) were contaminated with Listeria spp. L...
Source: Frontiers in Microbiology - January 29, 2024 Category: Microbiology Source Type: research

Inhibition of Polyphosphate Degradation in < em > Synechocystis < /em > sp. PCC6803 through Inactivation of the < em > phoU < /em > Gene
J Microbiol Biotechnol. 2024 Jan 8;34(2):1-8. doi: 10.4014/jmb.2311.11046. Online ahead of print.ABSTRACTPhosphorus is an essential but non-renewable nutrient resource critical for agriculture. Luxury phosphorus uptake allows microalgae to synthesize polyphosphate and accumulate phosphorus, but, depending on the strain of algae, polyphosphate may be degraded within 4 hours of accumulation. We studied the recovery of phosphorus from wastewater through luxury uptake by an engineered strain of Synechocystis sp. with inhibited polyphosphate degradation and the effect of this engineered Synechocystis biomass on lettuce growth. ...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Han-Bin Ryu Mi-Jin Kang Kyung-Min Choi Il-Kyu Yang Seong-Joo Hong Choul-Gyun Lee Source Type: research

Fates of attached E. coli o157:h7 on intact leaf surfaces revealed leafy green susceptibility
Food Microbiol. 2024 May;119:104432. doi: 10.1016/j.fm.2023.104432. Epub 2023 Nov 28.ABSTRACTLeafy greens, especially lettuce, are repeatedly linked to foodborne outbreaks. This paper studied the susceptibility of different leafy greens to human pathogens. Five commonly consumed leafy greens, including romaine lettuce, green-leaf lettuce, baby spinach, kale, and collard, were selected by their outbreak frequencies. The behavior of E. coli O157:H7 87-23 on intact leaf surfaces and in their lysates was investigated. Bacterial attachment was positively correlated with leaf surface roughness and affected by the epicuticular wa...
Source: Food Microbiology - January 15, 2024 Category: Food Science Authors: Mengyi Dong Maxwell J Holle Michael J Miller Pratik Banerjee Hao Feng Source Type: research

Fates of attached E. coli o157:h7 on intact leaf surfaces revealed leafy green susceptibility
Food Microbiol. 2024 May;119:104432. doi: 10.1016/j.fm.2023.104432. Epub 2023 Nov 28.ABSTRACTLeafy greens, especially lettuce, are repeatedly linked to foodborne outbreaks. This paper studied the susceptibility of different leafy greens to human pathogens. Five commonly consumed leafy greens, including romaine lettuce, green-leaf lettuce, baby spinach, kale, and collard, were selected by their outbreak frequencies. The behavior of E. coli O157:H7 87-23 on intact leaf surfaces and in their lysates was investigated. Bacterial attachment was positively correlated with leaf surface roughness and affected by the epicuticular wa...
Source: Food Microbiology - January 15, 2024 Category: Food Science Authors: Mengyi Dong Maxwell J Holle Michael J Miller Pratik Banerjee Hao Feng Source Type: research

Fates of attached E. coli o157:h7 on intact leaf surfaces revealed leafy green susceptibility
Food Microbiol. 2024 May;119:104432. doi: 10.1016/j.fm.2023.104432. Epub 2023 Nov 28.ABSTRACTLeafy greens, especially lettuce, are repeatedly linked to foodborne outbreaks. This paper studied the susceptibility of different leafy greens to human pathogens. Five commonly consumed leafy greens, including romaine lettuce, green-leaf lettuce, baby spinach, kale, and collard, were selected by their outbreak frequencies. The behavior of E. coli O157:H7 87-23 on intact leaf surfaces and in their lysates was investigated. Bacterial attachment was positively correlated with leaf surface roughness and affected by the epicuticular wa...
Source: Food Microbiology - January 15, 2024 Category: Food Science Authors: Mengyi Dong Maxwell J Holle Michael J Miller Pratik Banerjee Hao Feng Source Type: research